Computer related Ocular Syndrome (CROS) is a group of visual symptoms experienced in relation to the use of computers. Early identification of risk factors of CROS will be helpful in preventing the occurrence of CROS among computer users. The study aimed at assessing the Prevalence and Knowledge of computer related ocular syndrome among Bank employees. The objectives of the study were to estimate the prevalence and knowledge of computer related ocular syndrome, to assess the practice regarding prevention of CROS, and to find out the association between CROS and selected Socio demographic variables. The research approach adopted for the study was quantitative and data was collected from 220 participants. The research design was Cross- sectional design. The setting of the study was Kerala Banks of Thiruvananthapuram Corporation. Participants were selected through cluster sampling. The tool used for assessing socio demographic variables, prevalence of CROS and knowledge regarding CROS was interview schedule. Check-list was used to assess the practice for the prevention of CROS. Data analysis was carried out using both descriptive and inferential statistics. The prevalence of Computer related Ocular syndrome among Bank employees were, eye strain 67.30%, headache 60.50%, redness 29.10%, watering eye 27.7% and blurred vision 25.0%. Results showed the knowledge regarding computer related ocular syndrome, 41.4% of participants had good knowledge and 31.4% had poor knowledge. Practice regarding prevention of CROS, 41.4% had good practice and 31.4% had poor practice. The findings revealed that knowledge and visual problems are significantly associated with CROS.