In order to investigate the impact of fatigue damage on the ultimate load capacity of criticality cells in steel–concrete composite segments and to address the complex design challenges associated with bridge steel–concrete composite segments in practical engineering, this study designs two scaled criticality cell specimens with a scale ratio of 1:2 and performs ultimate load capacity tests after fatigue cyclic loading. By analyzing the stress distribution of each component and the force transmission ratio and combining the results from finite element model calculations, this study introduces the degree of structural fatigue damage and proposes a predictive model for the ultimate load capacity of steel–concrete composite segment criticality cells that is easy to apply. This model is compared with the finite element calculation results and experimental values, and the results are found to be in good agreement. Additionally, the number of shear connection members in the model is optimized based on the calculation results. The research findings indicate that the main failure mode of criticality cells is inclined compression failure. The strength of each part decreases in the following order: steel–concrete composite segment, steel structure segment, and concrete segment. Furthermore, fatigue damage has a significant impact on criticality cells. The optimized model exhibits similar stress performance and force transmission ratio to the original model and provides a reference for the design of practical engineering.
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