Zinc Compounds Research Articles

In‐Silico Screening, Molecular Dynamics, and DFT Analysis of ZINC and ChEMBL Library Compounds for SARS‐CoV‐2 Main Protease Inhibition

In‐Silico Screening, Molecular Dynamics, and DFT Analysis of ZINC and ChEMBL Library Compounds for SARS‐CoV‐2 Main Protease Inhibition

A family of zinc compounds of anthracene-appended new multifunctional organic scaffold as potent chemotherapeutics against cervical cancer

A family of zinc compounds of anthracene-appended new multifunctional organic scaffold as potent chemotherapeutics against cervical cancer

Factors of mild and moderate course of COVID-19 in non-immunized persons

Severity of COVID-19 is known to be related to various factors. The aim of the study was to perform comparative analysis of the features associated with mild and moderate course of infection caused by the SARS-CoV-2 virus in the absence of immunization against a new coronavirus infection, and looking for opportunities of binary classification. We performed the analysis of clinical and laboratory data of 59 medical staff members who had a mild (group No. 1), or moderate (group No. 2) forms of COVID-19. The results were analyzed using STATISTICA and GraphPad Prism programs. We have found that the patients with moderate course of the disease were 12% older, which numerically corresponded to a difference of 5 years (p = 0.019). Males were 1.5 times more likely to have a moderate course of the disease (p = 0.000). A part of respondents used non-specific immunoprophylactic agents. It was found that the intake of cholecalciferol, riamilovir/triazaverin did not affect the severity of disease, and the usage of human recombinant interferon alpha-2b in order to prevent SARS-CoV-2 virus infection was associated with subsequent mild course of COVID-19 (p = 0.000). More severe clinical course of COVID-19 was associated with simultaneous usage of several nonspecific immunoprophylactic agents, in particular, ascorbic acid, zinc compounds, umifenovir hydrochloride monohydrate (p = 0.000). Higher body mass index was also associated with a severe course of the disease (p = 0.034). The analysis showed that the issue of binary classification for the patients with mild and moderate COVID-19 in the absence of vaccination may be resolved (recognition accuracy is 90.9%, p 0.0001). The risk of moderate COVID-19 in non-immunized individuals is influenced by advanced age, gender factor, increased body mass index, presence of hypertension (70% hazard increase), as well as simultaneous use of several non-specific immunoprophylactic agents to prevent infection. Presence of allergic reactions and frequency of seasonal acute respiratory viral infections do not affect the severity of COVID-19. Prophylactic usage of human recombinant IFNa-2b is associated with milder course of infection caused by the SARS-CoV-2 virus.

A Review on Understanding Halitosis: Causes, Types, and the Role of Mouth Fresheners

Bad breath, or halitosis, is a common condition with significant social and psychological implications. Mouth fresheners, available in various forms such as sprays, chewing gums, lozenges, and rinses, offer a quick solution for managing this issue. This review provides a comprehensive analysis of the types, mechanisms, and effectiveness of mouth fresheners in tackling bad breath. It explores the role of natural and synthetic ingredients, including essential oils, antibacterial agents, and zinc compounds, in neutralizing odor-causing compounds and improving oral hygiene. The review also delves into traditional practices, emerging trends like probiotic and eco-friendly products, and the impact of mouth fresheners on the oral microbiome. While mouth fresheners provide immediate relief, their efficacy in addressing the root causes of halitosis remains limited, necessitating a holistic approach to oral care. Keywords- Halitosis, Bad Breath, Mouth Fresheners

Productive Potential of Bacillus and Pseudomonas Strains Grown in the Presence of Iron, Zinc and Copper Salts

Identifying and obtaining biotechnological solutions for agriculture, such as the development of antimicrobial agents with highly effective biological properties and plant productivity stimulants, are sustainable solutions, friendly to the environment and harmless to humans. Due to the importance of iron and zinc for plant growth and development, as well as copper used to protect plants against a wide range of fungal and bacterial diseases, studies have been initiated to identify and select new strains of microorganisms with the potential to synthesize bioactive substances when grown in the presence of iron, zinc and copper-based compounds. Thus, the effect of iron, zinc and copper compounds on the productivity of three strains of Bacillus sp. bacteria and three strains of Pseudomonas sp. with antimicrobial action on phytopathogens was tested.

Discovery of novel pyrrolo[2,3-d]pyrimidine derivatives as anticancer agents: virtual screening and molecular dynamic studies

ABSTRACT CDK/Cyclins are dysregulated in several human cancers. Recent studies showed inhibition of CDK4/6 was responsible for controlling cell cycle progression and cancer cell growth. In the present study, atom-based and field-based 3D-QSAR, virtual screening, molecular docking and molecular dynamics studies were done for the development of novel pyrrolo[2,3-d]pyrimidine (P2P) derivatives as anticancer agents. The developed models showed good Q 2 and r 2 values for atom-based 3D-QSAR, which were equal to 0.7327 and 0.8939, whereas for field-based 3D-QSAR the values were 0.8552 and 0.6255, respectively. Molecular docking study showed good-binding interactions with amino acid residues such as VAL-101, HIE-100, ASP-104, ILE-19, LYS-147 and GLU-99, important for CDK4/6 inhibitory activity by using PDB ID: 5L2S. Pharmacophore hypothesis (HHHRR_1) was used in the screening of ZINC database. The top scored ZINC compound ZINC91325512 showed binding interactions with amino acid residues VAL-101, ILE-19, and LYS-147. Enumeration study revealed that the screened compound R1 showed binding interactions with VAL 101 and GLN 149 residues. Furthermore, the Molecular dynamic study showed compound R1, ZINC91325512 and ZINC04000264 having RMSD values of 1.649, 1.733 and 1.610 Å, respectively. These ZINC and enumerated compounds may be used for the development of novel pyrrolo[2,3-d]pyrimidine derivatives as anticancer agent.

Effect of Solution Concentration on the Electrochemical Properties of Electrospun Zinc Phosphate Carbon Nanofibers As Anode Materials for Lithium-Ion Batteries

Zinc (Zn) is notable for its abundance and environmental friendliness, offering various compounds like zinc oxide, sulfide, selenide, phosphides, and phosphates [1]. While zinc oxide and chalcogenides are well-researched for their use in lithium-ion battery anodes, other zinc compounds, including phosphides and phosphates, remain largely unexplored [2]. Despite limited research on zinc phosphate as an anode material, its unique properties have been recognized across different applications [3,4]. Exploring the synergy between phosphide and phosphate presents an exciting opportunity for advancements.This work investigates carbon composite nanofibers of zinc phosphates synthesized using the facile electrospinning method directly from polyvinylpyrrolidone (PVP)-containing precursors. The impact of the electrospinning solution on the composition and morphology of the prepared materials was studied. Furthermore, the potential of zinc phosphate and its coupled forms as lithium-ion battery anode materials was elucidated.Electrospinning solutions were prepared by varying concentrations of zinc nitrate, PVP and phosphoric acid to determine the maximum spinnable concentration with the best physical and electrochemical properties of final samples and electrospun at specified electrospinning parameters. Obtained fibers were dried, pre-oxidized, annealed for 1 h at 750°C in N2 + H2 (4%), and denoted as ZnPO/C samples.Formation of the desired materials was confirmed by X-Ray diffraction (XRD, Miniflex, Rigaku) and scanning electron microscopy (SEM, Crossbeam500, Zeiss).The fibrous morphology of the ZnPO/C nanofibers confirmed from SEM images in Figure (a).It also shows homogeneity in fiber shape and size across all concentrations. XRD patterns of ZnPO/C samples of varying concentrations in Figure (b) have shown formation of Zn2P2O7 with some ⍺-Zn3(PO4)2 impurities.The intensities showed an increase corresponding to the concentration of the samples, along with some degree of peak broadening. It might be due to the amorphous carbon regions present in higher concentrations.Further details of the impact of the concentration on physical and electrochemical properties of the prepared materials will be presented at the conference. Acknowledgements This research was funded by the projects AP19675260 “Development of nanofibrous electrode materials for next-generation lithium-ion batteries” and AP13068219 “Development of multifunctional free-standing carbon composite nanofiber mats” from the Ministry of Education and Science of the Republic of Kazakhstan.[1] Y. Zhang, J. Wang, M. Alfred, P. Lv, F. Huang, Y. Cai, H. Qiao, Q. Wei,Energy Storage Materials 51 (2022) 2405-8297[2] W. Li, L. Gan, K. Guo, L. Ke, Y. Wei, H. Li, G.Shen, T. Zhai, Nanoscale 8 (2016) 8666-8672[3]Y. Guo, Y. Liu, Y. Li, S. Hou, T. Liu, L. Zhao, Self-assembly of ZnO nanoparticles attached to 3D pleated Zn3(PO4)2/C from cola for enhanced lithium storage, Appl Surf Sci 508 (2020) 145288.[4]Y. Li, Z.T. Wang, G. Liu, J. Wang, J. Wang, Boosting the electrochemical performance of LiNi0.8Co0.1Mn0.1O2 cathode materials with Zn3(PO4)2 surface coating, Advanced Powder Technology 32 (2021) 4651–4657. Figure 1

Electrochemical Characteristics of Rechargeable MnO x /Zn Battery

The charge/discharge characteristics of several types of manganese oxides with different structures as positive electrodes for zinc negative electrode batteries were evaluated. It is said that MnOOH is formed in the discharge reaction of γ-MnO2, but when the charge/discharge reaction was performed using β-MnO2 as the positive electrode, the formation of Mn2O3 and Mn3O4 instead of MnOOH was observed in the first discharge reaction. After the second cycle, no reversible reformation of β-MnO2 due to charging was observed. Furthermore, due to the charge/discharge reaction, zinc was dissolved from the zinc negative electrode, and basic zinc compounds were observed as a by-product in both the positive and negative electrodes. After the second cycle, the amount of Mn2O3 and Mn3O4 produced increased and decreased each time the discharge and charge were repeated. The discharge capacity showed a stable discharge capacity of ca.200 Ahkg-1 until about 30 cycles, but after that it gradually decreased to 105 Ahkg-1 at 100 cycles.

Therapeutic Dose of Zinc Aspartate and Zinc Citrate Attenuates Disease Activity Indices in Rheumatoid Arthritis.

Zinc aspartate and zinc citrate have been used as zinc supplements in different health conditions. Taking into consideration their anti-inflammatory, immunomodulatory, anti-oxidant and antimicrobial properties, the present study has been designed to analyse the effect of zinc aspartate and zinc citrate treatment at therapeutic dose level on disease severity index, haematological, serological, antimicrobial and radiological markers of rheumatoid arthritis in Wistar rats. Bactericidal potential of the two organic zinc compounds was analysed in vitro in clinically isolated Escherichia coli. Arthritis was induced in male Wistar rats by intradermal injection of an emulsion containing collagen type II and Complete Freund's Adjuvant (CFA) containing 1mgmL-1 Mycobacterium tuberculosis H37Ra. Zinc aspartate and zinc citrate were orally administered after the onset of the disease for 4weeks. Ameliorative effect of zinc aspartate and zinc citrate was evaluated by analysing indices of severity and disease activity markers of rheumatoid arthritis. The liver and kidney function tests were performed to evaluate any possible adverse effect of compounds. Antimicrobial activity of the zinc compounds was assessed in clinically isolated E. coli by MTT assay. Zinc aspartate and zinc citrate equivalent to a therapeutic dose of 50mg/day of elemental zinc attenuated the clinical characteristic of rheumatoid arthritis in the animal model of arthritis, collagen-induced arthritis (CIA). Both zinc salts also exhibited antimicrobial effects against E. coli. The selected dose of zinc aspartate and zinc citrate showed no adverse effects in treated rats. This study highlights the potentiality of zinc compounds as antiarthritic agents and also point to its preventive effects on microbial growth that has been observed in rheumatoid arthritis patients due to their increased sensitivity for bacterial infection.

Evaluation of foliar fungicides for Cercospora leaf spot management in table beet in New York

Cercospora leaf spot (CLS), caused by the fungus, Cercospora beticola, is a major constraint to table beet production in New York (NY). The disease causes defoliation, negatively impacting harvests using top-pulling machinery and rendering plants unsuitable for fresh market sales. High genetic diversity within C. beticola populations can result in the rapid development of resistance to single-site mode of action fungicides. Therefore, products with multiple modes of action and/or differential site-specific modes of action are highly desirable to improve the resiliency of CLS management. In 2021 and 2022, small plot replicated trials were conducted to evaluate the efficacy of selected fungicides for CLS management in table beet at Geneva, NY. Propiconazole, pydiflumetofen + fludioxonil, and copper hydroxide were the most efficacious for CLS management and not significantly different between each other. Across these treatments, CLS severity was reduced by 72.8% and 78.6% compared to nontreated plots in 2021 and 2022, respectively. Trifloxystrobin + fluopyram, pyrimethanil + fluopyram and pyraclostrobin + fluxapyroxad provided moderate disease control with an average reduction of 45.4% and 56.7% in CLS severity in 2021 and 2022, respectively. Trifloxystrobin and a proprietary copper + zinc (Cu/Zn) compound at two rates (0.25% and 0.5% v/v) also significantly reduced CLS severity by 20.1% in 2021 but not in 2022. Improvements in foliar health through reduced CLS severity had no significant effect on root shape, size, color, and sugar content. This information underpins best management practices for foliar health leading to economic and sustainable outcomes for table beet producers.

Coordination compounds of zinc carboxylates with an unusual benzohydrazide binding mode

Two new coordination compounds [Zn(Bhz)4(p-OB)2] (1) and [Zn(Bhz)3][Zn(Bhz)2(Fum)2].2H2O (2) (Bhz – benzohydrazide, p-OB - p-hydroxybenzoate, Fum - fumarate anions) have been synthesized and characterized by elemental analysis, IR spectroscopy, mass spectrometry and single-crystal X-ray diffraction.The results obtained show that the nature of the anion is very important factor in the complexes construction and strongly defines the coordination mode of hydrazide and acid ligands. Coordination compounds of zinc with monodentate benzohydrazide were synthesized for the first time.

Effect of Nanoclay Modification on Properties of Natural Rubber Nanocomposites Using Zinc Compound

This study investigates the effect of nanoclay modification on the properties of natural rubber (NR) nanocomposites using zinc stearate (ZS) and di(hydrogenated tallow) dimethylammonium chloride (DHDT). The aim is to enhance the compatibility of modified nanoclay (NC) and NR molecules for determining cure characteristics, mechanical properties, and morphologies. The effect of unmodified and modified NC as the secondary filler was studied using the 10 phr NC regarding the received properties of the silica-based composites. Results indicated that the NR composites with modified NC exhibited a significant improvement of 32.06% estimated crosslink density, 16.6% tensile strength, 30.1% Payne effect together with the production time of 3.9 min relative to the composites with unmodified NC. This related to the dispersion and distribution degree of the NC and it suggests that the modification of nanoclay with ZS and DHDT can positively impact to the NR nanocomposites, enhancing their mechanical performance and processing characteristics. Keywords: Modified nanoclay, Natural rubber, Nanocomposites

Оценка степени высвобождения и токсичности соединений цинка, используемых в фармацевтических и косметических средствах

The degree of release from ointments and the toxicity of three zinc compounds were assessed to establish their minimum sufficient concentration in antibacterial pharmaceutical and cosmetic products. The properties of zinc oxide (ZnO), zinc pyrrolidone carboxylate (ZnРСА) and zinc salicylate (Zn(Sal)2·2H2О) were compared. The release rate of active substances from petrolatum-based ointments (the mass fraction of zinc compounds is 1%) was determined by the Kruvchinsky method. A cellophane film for dialysis served as a semipermeable membrane, which was fixed on a glass tube (length 15 cm, cross section 20 mm2). A sample of ointment (2.00 g) was applied to the inner surface of the membrane. The dialysis tube was inserted into a beaker with dialysis medium and immersed to a depth of no more than 3 mm. The toxicity of aqueous solutions (distilled water) of zinc compounds was determined in a bioluminescent biotest using “Ecolume” bacterial preparation based on a non-pathogenic strain of Escherichia coli. It was found that a high level of toxicity of is achieved at 10-5 mol/dm3 zinc salts in a solution, zinc oxide – 10-4 mol/dm3. A similar amount of Zn2+ diffuses from ointments through a semipermeable cellophane membrane used for the study of dialysis into a 50% aqueous alcohol solution. Zinc ion release from ointment with ZnO was only 0.31%, as well as from ointments with ZnPCA and Zn(Sal)2·2H2О – 1.59% and 1.87% respectively. Thus, the tested approach is economically and environmentally effective, as it allows you to determine the lowest effective concentration of active substances, reducing the burden on the human microbiome and the environment.

Iron, Zinc and Total Phenolic Content in Cooked Biofortified NUA 45 and Gloria Sugar Beans: The Case of Zimbabwe

Biofortification involves breeding of staple crops that are micronutrient-dense and high yielding for example iron-biofortified beans. The biofortification of crops has been found to be cost effective and feasible to fight micronutrient deficiencies. The aim of the study was to determine the iron, zinc and total phenolic composition for the biofortified NUA 45 and Gloria sugar beans in raw as well as cooked samples with and without previous soaking. Determination of iron and zinc content in the raw, cooked bean grain samples with and without soaking was carried out by Inductively Coupled Plasma (ICP) Optical Emission Spectrometry whereas the determination of Total Phenolic Content was done using the Folin-Ciocalteau colorimetric method. The highest zinc concentration (0.295ppm) was found in raw Gloria sugar beans whilst the raw NUA 45 sugar beans had the highest iron content (0.755ppm) and total phenolic composition (352.8mg/100g). For the cooked bean samples Gloria sugar beans had the highest zinc concentration (0.247ppm) cooked after soaking. NUA 45 sugar beans had no significant difference on the zinc content after being cooked with and without soaking. Amongst the cooked samples the biofortified NUA 45 sugar beans cooked after soaking had the highest iron content (0.373ppm) as well as the highest total phenolic content in the beans cooked without soaking (200.5mg/100g). Biofortified NUA 45 and Gloria sugar beans proved to be good sources of iron, zinc and phenolic compounds. However, cooking with and without soaking diminished the iron, zinc and the total phenolic composition of the cooked NUA 45 and Gloria sugar beans.

Structure, Adsorptive and Photocatalytic Properties of Porous ZnO Nanopowders Modified by Oxide Compounds of Manganese

Porous nanocomposites based on oxide compounds of zinc and manganese are synthesized and their structure, morphology, spectral and photocatalytic properties are studied. It is shown that the resulting porous oxide composites have photocatalytic properties and consist of ZnO, Mn3O4 and ZnMn2O4 nanocrystals with a size of 20–40 nm. The introduction of Mn2+ ions into the crystal lattice of ZnO causes a increase in the size of the unit cell of crystals. The band gap of the composites is 3.26 eV. The kinetics of photocatalytic decomposition in a Chicago Blue Sky dye solution is described by a pseudo-first order equation. In the presence of porous nanocomposites, the processes of oxidation of organic compounds proceed both on the surface of photocatalysts and in solution. The synthesized nanocomposites are promising for use in photocatalytic systems for water purification from organic contaminants.

In-silico identification of 3,4-Diarylpyrazoles-based small molecules as potential Hsp90 inhibitors

The ATP-dependent molecular chaperone, Hsp90, is crucial for the activity of hundreds of client proteins. Several of Hsp90 clients are involved in cancer cell growth and proliferation. Thus, numerous Hsp90 inhibitors have been developed to target Hsp90′s ATPase activity in cancer cells. CCT018159 is a pyrazole-based compound that successfully inhibited Hsp90 oncogenic activity by binding deeply to the ATP binding pocket in the Hsp90 N-terminal domain (N-Hsp90). In the present study, we utilized the CCT018159 structure to identify new potential Hsp90 inhibitors using a set of in-silico approaches. The ZINC database was virtually screened for CCT018159 analogs using the SwissSimilarity webpage. Ten molecules were selected for further investigation based on their drug-likeness, pharmacokinetics, and physiochemical properties. The molecular docking simulation analysis revealed that these ZINC compounds have Ligand Binding Energies (LBEs) ranging from −8.61 to −9.73 Kcal/mol, which reflects a high binding affinity compared to CCT018159 (LBE of −6.31 Kcal/mol). These compounds exhibited diverse interactions, including critical hydrogen bonds and hydrophobic contacts with crucial residues like Asn51, Ala55, and Leu107. Further optimization targeting unique interactions (e.g., ZINC000008973382 with Asp93, Met98, and Thr184) and exploiting the versatile Lys58 could lead to novel, potent anticancer agents targeting Hsp90.

Hydrochemical features of the Tuzlov River as an indicator of the consequences of industrial and agricultural activities in Eastern Donbass (Rostov-on-Don Region, Russia)

The spatial and temporal variability of solute transport, chemical composition and water quality in the Tuzlov River basin for the period 2003–2019 has been considered. The purpose of this study was to consider the Tuzlov basin as an indicator of the consequences of economic activity in the territory of Eastern Donbass. The results obtained on the transport of dissolved substances in the upper part of the basin showed an increase in the transport of major ions, on the contrary, a decreasing trend in the transport of copper, zinc and phosphorus phosphate compounds. Further, the variability of water chemistry in the middle section of the basin, as well as at the observation sites above and below Novocherkassk was considered. In the middle reaches, the increase in the content of major ions continued, and a sharp increase in the concentration of phosphorus phosphate was recorded. The tendency to reduction of copper and zinc compounds remained the same. Below Novocherkassk — at the closing site of hydrochemical observations — the content of phosphorus phosphates, copper and zinc compounds increased to the greatest extent. Water quality during the period under consideration remained at the level of class 4 (“dirty”), its spatial and temporal variability was insignificant. Based on the results obtained, conclusions were made about the leading role of the consequences of coal mining in the increase in the content of major ions (primarily due to sulfate ions) and total iron compounds in water. At the same time, agricultural activities in the catchment lead to a decrease in the content of copper, zinc and phosphorus phosphate compounds in water. However, at the most urbanized Tuzlov River site in the city of Novocherkassk, the content of these components in water increased significantly. The results of the study may be useful in planning conservation measures in the studied area.

Antibacterial coating for orthodontic materials

ABSTRACT In recent years, there has been a growing interest in the development of antibacterial coatings to prevent biofilm formation. Innovative surface modification techniques such as silica coating have been shown to effectively reduce the toxicity of nanoparticles while maintaining their antimicrobial effects. This has significant implications for the biocompatibility of antimicrobial nanoparticles in various applications, including antimicrobial surfaces and additives in food products. Traditional materials such as titanium oxide, silver and silver compounds, zinc and zinc compounds, zirconium and zirconium compounds, and gold and gold compounds with continuous advancements in antibacterial coating technologies, it is imperative to achieve long-term activity to ensure the efficacy of these coatings in clinical practice. This necessitates addressing specific material characteristics, coating architecture, and surface coverage to improve the long-term activity of antibacterial coatings.

Exposure to zinc and dialkyldimethyl ammonium compound alters bacterial community structure and resistance gene levels in partial sulfur autotrophic denitrification coupled with the Anammox process

Dialkyldimethyl ammonium compound (DADMAC) is widely used in daily life as a typical disinfectant and often co-exists with the heavy metal zinc in sewage environments. This study investigated the effects of co-exposure to zinc (1 mg/L) and DADMAC (0.2–5 mg/L) on the performance, bacterial community, and resistance genes (RGs) in a partial sulfur autotrophic denitrification coupled with anaerobic ammonium oxidation (PSAD-Anammox) system in a sequencing batch moving bed biofilm reactor for 150 days. Co-exposure to zinc and low concentration (0.2 mg/L) DADMAC did not affect the nitrogen removal ability of the PASD-Anammox system, but increased the abundance and transmission risk of free RGs in water. Co-exposure to zinc and medium-to-high (2–5 mg/L) DADMAC led to fluctuations in and inhibition of nitrogen removal, which might be related to the enrichment of heterotrophic denitrifying bacteria dominated by Denitratisoma. Co-exposure to zinc and high concentration DADMAC (5 mg/L) stimulated the secretion of extracellular polymeric substances and increased the proliferation risk of intracellular RGs in sludge. This study provided insights into the application of PSAD-Anammox system and the ecological risks of wastewater containing zinc and DADMAC.

A zinc phosphate layered biodegradable Zn-0.8Mg-0.2Sr alloy: Characterization and mechanism of hopeite formation

The objective of this study is to comprehensively investigate the formation mechanism of hopeite on a promising biodegradable Zn-0.8Mg-0.2Sr alloy during its self-corrosion process and its subsequent influence on the interaction of the material with cells and bacteria. Notably, the self-corrosion process of Zn-based alloys inherently generates Zn2+ ions, eliminating the necessity for additional zinc compounds in the treatment. The investigation focused on the chemical composition of the applied baths, exposure time, and temperature, in order to analyze the structure, quality, distribution, phase composition, thickness, and roughness of the resulting layers bringing range of key pros and cons for suggested materials. While the thin layers are considered more beneficial in a wide range of applications, the resulting thinnest layer was observed after exposure to the bath containing additives at 50 °C for 24 h, measuring approximately 192 ± 21 pm in thickness. In addition, the layer possessed the most homogeneous distribution under these conditions. Generally, the layers formed in the bath without additives exhibited higher thickness and rougher surfaces compared to those in the bath containing additives. Such results had a direct impact on dissolution rate, cytotoxicity, and antibacterial properties. The highest dissolution rate (40.7 mg·cm−2·day−1) indicating a limited lifetime of the layer was obtained for material processed under the following coating condition: 50 °C, 24 h, and bath without additives. The general presence of surface layers manifested in increased cell viability under direct cytotoxicity test, while smoother surface conditions also supported the antibacterial properties, both making suggested treatments as interesting variations for biodegradable zinc-based materials.