Composition Of Phenolic Compounds Research Articles

Effect of steam explosion pretreatment on the composition and bioactive characteristic of phenolic compounds in Chrysanthemum morifolium Ramat cv. Hangbaiju powder with various sieve fractions.

Steam explosion (SE) pretreatment is an efficient technique to promote the fiber degradation and disrupt materials' cell wall. In this study, the effect of SE pretreatment on the changes in phenolic profile, and the in vitro digestion property of a Chinese indigenous herb “Hangbaiju” (HBJ) powder with various sieve fractions (150‐, 180‐, 250‐, 425‐, and 850‐μm sieves) were studied. After SE pretreatment, the morphological structure, color attributes, and composition of phenolic compounds were altered significantly (p < .05). The composition and content of phenolic compounds were strongly correlated with particle sizes. The higher extraction yield of phenolic compounds was reached in the intermediate sieve fraction (ca. 250‐μm sieves). During in vitro digestion, the changes in phenolic compounds were significant due to the transition from an acidic to the alkaline environment (p < .05). Based on the multivariate statistical analysis, apigenin‐7‐O‐glucoside, luteolin‐7‐O‐glucoside, and linarin, were viewed as the characteristic compounds among various samples. The results highlighted that the phytochemical properties mainly including the composition of phenolic compounds, and in vitro digestion properties of HBJ powder with intermediate sieve fraction could be improved after SE pretreatment.

Phenolic compounds composition and in vitro antioxidant activity of Nigerian Amaranthus viridis seed as affected by autoclaving and germination

Due to the rise in several diseases and conditions, there has been the need for food that possesses supplemental health advantages asides its nutritional advantages. This study evaluates total phenolics, total flavonoids, total antioxidants, and antioxidant activity measured in DPPH radical scavenging activity (DPPH), ferric reducing antioxidant power (FRAP) and metal chelating activity (MCA) of methanol extract of Amaranthus viridis seed with the view of utilizing it as functional food or ingredient. There was also the identification and quantification of phenolic as well as flavonoid compounds with the use of high-pressure liquid chromatography (HPLC). The extracts of amaranth seed exhibited a total phenolic content of 107.89, 95.26 and 164.73 mg tannic acid equivalent/100 g extract for raw, autoclaved and germinated Amaranthus viridis seed respectively. A comparable trend was observed in the total flavonoid as well as the antioxidant content. The strongest radical scavenging activities (48.63 mg tannic acid equivalent (TE)/100 g extract), FRAP (mg Fe (II)/100 g extract) and metal chelating ability (mg ethylenediamine tetraacetic acid/g extract) was observed in germinated flour. An abundance of ferulic and para-hydroxybenzoic acid was observed among the phenolic compounds of the seed of the amaranth.The high efficacy of the seeds of Amaranthus viridis cultivated in Nigeria was demonstrated in this work and it recommends the functionality of the seeds as an important natural antioxidant source.

Effect of the Addition of Different Levels of Chard on the Dough Properties and Physicochemical and Sensory Characteristics of Pan Breads

Background. Chard is a valuable vegetable and is considered a beneficial functional food. Fortification of bread with chard could increase the nutraceutical and functional food consumption. Objective. In this study, we performed a chemical analysis of chard and performed rheological analyses and sensory attribute evaluations of pan breads fortified with 5% and 10% chard powder. Design. The gross chemical composition of chard, some minerals, vitamin C, and total phenolic and flavonoid compounds were estimated. The rheological properties of doughs fortified with 5% and 10% chard powder and the chemical composition and sensory attributes of control, 5% chard and 10% chard pan bread samples were determined. Results. Chard contains carbohydrate, protein, and ash in addition to essential minerals and antioxidants such as vitamin C, phenols, and flavonoids. The chemical composition of 5% chard pan bread was significantly higher in ash and fiber, while the chemical composition of 10% chard pan bread was significantly higher in protein, ash, fiber, and moisture and significantly lower in fat, carbohydrate, and energy level than that of control pan breads. Compared with the control pan bread, the pan bread with increased chard powder content (10%) had significantly increased water absorption percentage, arrival time, dough development, elasticity, and proportional number ratio but significantly decreased stability time, softening degree, and extensibility. Pan bread fortified with 10% chard had the lowest specific volume among the tested breads. Sensory attribute evaluation further showed that increasing the amount of chard to 10% in the bread dough formulation produced lower overall acceptability scores. Conclusions. Pan bread containing 5% chard had better rheological scores and sensory attributes than the other formulations, in addition to good nutritional quality values.

PHENOLIC COMPOUNDS IN LEAVES AND RACEMES HEDYSARUM ALPINIM L. AND H. FLAVESCENS REGEL ET SCHMALH., INTRODUCED INTO THE FOREST-STEPPE ZONE OF WESTERN SIBERIA

The change in the composition and content of phenolic compounds in the leaves and racemes of Hedysarum alpinim L. and H. flavescens Regel et Schmalh., introduced into the forest-steppe zone of Western Siberia were studied. On the example of H. alpinim, the changes in the content of phenolic compounds depending on the year of harvest and on the plant organ (leaves, racemes) were studied. The content of individual substances (gallic, chlorogenic acids, mangiferin, hyperoside, rutin, avicularin and 17 components of a phenolic nature) and the content of phenolic compounds by groups (hydroxycinnamic and hydroxybenzoic acids, xanthones and flavonols) were determined. Differences in the content of phenolic compounds by groups (in mg / 100 g) were found between the two species of the genus Hedysarum L.: xanthones and flavonols (up to 4614.9±63.7 and 1280.0±6.7, respectively) in leaves and xanthones (up to 847.9±29.8) in racemes in H. alpinim accumulated more than xanthones and flavonols (2911.6±79.0 and 976.2±0.5, respectively) in leaves and xanthones (76.2±2.5) in racemes H. flavescen. In the leaves of H. flavescens, in comparison with the leaves of H. alpinim, the content of hydroxybenzoic and hydroxycinnamic acids prevails. The leaves of the studied species accumulate the most hydroxycinnamic acids and xanthones, while the racemes accumulate the most flavonols. The content of hydroxybenzoic acids, xanthones, and flavonols H. alpinim varied significantly depending on the year of observation.

Extraction, Chemical Composition and Antioxidant Activity of Phenolic Compounds from Moroccan Satureja nepeta L.

Satureja nepeta is an aromatic medicinal plant, widely used by the Moroccan population. The objective of this study is to evaluate the influence of extraction methods on total phenolic compounds (TPC) and total flavonoid compounds (TFC). The phytochemical characteristics are subsequently analyzed to determine the components constitution as well as the evaluation of antioxidant activity. The extraction optimization and the extraction of total polyphenols was carried out using three methods (decoction, infusion and hydroethanolic). The antioxidant capacity was evaluated using the DPPH free radical trapping method, reducing power assay and the ABTS radical reduction test. Polyphenols were identified by the HPLCUV method. The optimal hydro-ethanol extraction conditions defined from the experimental design are: ethanol/water, 60/40 (v/v), 15 hours, 42.5 °C TPC and TFC of the infused extract were the highest. The evaluation of the antioxidant capacity showed that the infused extract had a good antioxidant efficacy. HPLC-UV and GC-MS has been used to identify some phenolic compounds in various extracts. In conclusion, optimizing the extraction of S. nepeta seems to be an efficient and quick method to establish the best extraction conditions. Also, Satureja. Key words: Moroccan Satureja nepeta, Antioxidant activity, Total polyphenol, Total flavonoids, Total tannins.

POSTHARVEST BIOCHEMICAL CHANGES IN APPLE SAMPLES

Apples are seasonal fruits, and it is relevant to prepare them adequately for storage and ensure proper storage conditions. In this study, we used ‘Rubin’ apple cultivar. We studied the qualitative and quantitative composition of phenolic and triterpenic compounds in the apple before placing them in the controlled atmosphere chambers and against at the end of the experiment, eight months later. Different concentrations of oxygen, carbon dioxide and nitrogen, constant temperature, relative humidity, and removal of endogenous ethylene were continually maintained. HPLC analysis showed that in the apple samples stored of variant II (5 % - O2, 1 % - CO2, 94 % - N2) conditions the total amount of triterpenes changed the least, dropped by 20 %. The study showed that the total content of phenolic compounds has increased in apple samples stored under controlled atmospheric conditions. The highest content of phenolic compounds increased in apple samples stored under IV (5 % - O2, 5 % - CO2, 90 % - N2) conditions. Evaluating the optimal storage parameters is useful to providing the consumers with apples that have a known and minimally altered phytochemical composition of phenolic compounds and triterpenes, which estimate the use of apples in the healthy food chain.

Phenolic compounds profile and antioxidant capacity of ‘Ataulfo’ mango pulp processed by ohmic heating at moderate electric field strength

Heat treatment during pasteurization of mango (Mangifera indica L.) pulp reduces the phenolic content and its potential health benefits. The bioactive content, phenolics profile, and antioxidant capacity of ‘Ataulfo’ mango pulp after ohmic heating (OH) treatment (15.0–20.0 V/cm), and conventional heating (CT, 72 °C) were evaluated. No significant differences were observed in the gallic acid and mangiferin content and its antioxidant capacity (ABTS). Mass spectrometry analysis (LC/MS-TOF) showed that all treatments produced the same profile of phenolic compounds, including 6 phenolic acids, 2 gallotannins, 1 benzophenone, 2 xanthones, and 3 flavonoids. PCA analysis confirmed that mangiferin and gallic acid were the main contributors to the ABTS antioxidant capacity. These results demonstrate that OH treatments can preserve the compositions of phenolic compounds mango pulp, thus maintaining its potential health benefits.

Papaver Plants: Current Insights on Phytochemical and Nutritional Composition Along with Biotechnological Applications.

The genus Papaver is highly esteemed in the pharmacy industry, in the culinary field, and as ornamental plants. These plants are also valued in traditional medicine. Among all Papaver species, Papaver somniferum L. (opium poppy) is the most important species in supplying phytochemicals for the formulation of drugs, mainly alkaloids like morphine, codeine, rhoeadine, thebaine, and papaverine. In addition, Papaver plants present other types of phytochemicals, which altogether are responsible for its biological activities. Therefore, this review covers the phytochemical composition of Papaver plants, including alkaloids, phenolic compounds, and essential oils. The traditional uses are reviewed along with their pharmacological activities. Moreover, safety aspects are reported to provide a deep overview of the pharmacology potential of this genus. An updated search was carried out in databases such as Google Scholar, ScienceDirect, and PubMed to retrieve the information. Overall, this genus is a rich source of alkaloids of different types and also contains interesting phenolic compounds, such as anthocyanins, flavonols, and the characteristic indole derivatives nudicaulins. Among other pharmacological properties, numerous preclinical studies have been published about the analgesic, anticancer, antimicrobial, antioxidant, and antidiabetic activities of Papaver plants. Although it highlights the significant impact of this genus for the treatment of a variety of diseases and conditions, as a future prospect, characterization works accompanying preclinical studies are required along with clinical and toxicology studies to establish a correlation between the scientific and traditional knowledge.

Biologically active compounds of berries and leaves of &lt;i&gt;Vaccinium corymbosum&lt;/i&gt; L.

Quantitative content of the sum of phenolic compounds and anthocyanins in their composition were study. The level of antiradical activity (ARA) in the berries and leaves ofVaccinium corymbosumL. was measured.The maximum content of the sum of phenolic compounds was observed in Blueray, Bluerose, Caroline Blue, Herbert, Jersey, Nelson cultivars. In the cultivars which have been studied the quantity of anthocyanins in the composition of phenolic compounds ranged from 0.01 to 0.8 % in green berries, and from 22 to 52 % in mature ones. Earlyblue, Duke, Bluecrop, Bluegold cultivars were characterized by the greatest quantity of phenolic compounds in leaves.The presence of a close positive correlation between the level of antiradical activity and the content of secondary metabolites of phenolic nature was determined. The differences in phenolic substances accumulation in blueberry cultivars obtained byin vivoandin vitromethods were revealed.It was shown that the proteomic profile of the highbush blueberry plants together with genetic passports can being used to determine the cultivar's identity ofVaccinium corymbosumL.

The regulating effect of light on the content of flavan-3-ols and derivatives of hydroxybenzoic acids in the callus culture of the tea plant, Camellia sinensis L

The objective was the study of the regulating effect of light on the composition and contents of phenolic compounds in the callus culture of the tea plant (Camellia sinensis) grown for 28 days in the light (16-h photoperiod) or in the dark. For the analyses of phenolic compounds, an ultra-performance liquid chromatography in combination with a photodiode array detector and a high-resolution Q Exactive Orbitrap mass spectrometry (UPLC-PDA-HRMS/MS) was used. Nineteen phenolic compounds were characterized. The main phenolic compounds were flavan-3-ols (mono- and oligomers of flavan-3-ols) and derivatives of hydroxybenzoic acids (mono- and digalloyl glucoses, hexosides of 2,5- and 2,3-dihydroxybenzoic and salicylic acids). It was shown that the growth of the tea callus in the light or in the dark does not affect the composition of phenolic compounds, but differently affects the content of flavan-3-ols and derivatives of hydroxybenzoic acids. In the callus grown in the light, the content of flavan-3-ols was about 2-fold higher than in the dark. On the contrary, the content of derivatives of hydroxybenzoic acids in the light was at average 3-fold lower. The regulating role of light in the biosynthesis of phenolic compounds, as well as light control of the distribution of carbon flux between the branches of the shikimate pathway, which are associated with the biosynthesis of flavan-3-ols and hydroxybenzoic acids, are discussed.

Antioxidant potential of two Brazilian seaweeds in response to temperature: Pyropia spiralis (red alga) and Sargassum stenophyllum (brown alga)

Natural antioxidants found in macroalgae represent a significant protective role against oxidative damage. The antioxidant capacity responses to abiotic stresses allow understanding the tolerance and sensitivity of the species. Pyropia and Sargassum inhabiting the intertidal zone exhibit several antioxidant mechanisms against oxidative stress stimulated by environmental factors, such as thermal stress. In this study, we evaluated the antioxidant capacity under different temperatures of the red macroalga Pyropia spiralis and the brown macroalga Sargassum stenophyllum, associated with the composition of mycosporine-like amino acids (MAAs) and phenolic compounds. Antioxidant capacity was determined by four antioxidant assays (ABTS: 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid); DPPH: 2,2-diphenyl-1-picryl-hydrazyl; FRAP: ferric reducing antioxidant power, and metal chelating). Phenolic compounds content was assessed using Folin-Ciocalteau reagent and HPLC, and MAAs using HPLC. In both species, the ABTS assay was the most sensitive antioxidant assay. At low temperature (15 °C), P. spiralis exhibited the lowest EC50 (half maximal effective concentration) in the ABTS assay, the highest total antioxidant capacity index, and high content of MAAs (porphyra-334, shinorine, and palythine). For S. stenophyllum, the lowest EC50 value for ABTS assay was registered at 15 °C, and the lowest total antioxidant capacity index at 30 °C with high content of phlorotannins, probably phloroethols. The information provided by this research may be useful for the study of thermal tolerance and sensitivity in marine macroalgae as well as a template for further investigations of designing phytochemicals in marine macroalgae.

Phenolic Content and Antioxidant Activity in Apples of the 'Galaval' Cultivar Grown on 17 Different Rootstocks.

Apple cultivars are one of the main factors setting the composition of bioactive compounds in apples and the quality of the fruit. However, research has been providing increasing amounts of data on the influence of rootstocks on the variations in the composition of bioactive compounds in apples. The aim of the study was to determine the influence of rootstocks on the changes in the qualitative and quantitative composition of phenolic compounds and their antioxidant activity in vitro in apple flesh and peel. HPLC analyses of phenolic compounds in apple samples were performed. The rootstock–scion combination had a significant effect on the composition and antioxidant activity of phenolic compounds in apple samples. Depending on the rootstock, the total content of phenolic compounds in apple flesh of the ‘Galaval’ cultivar could vary by 2.9 times, and in the peel by up to 90%. The genotype of the rootstock resulted in the highest variation in total flavan-3-ol content in apple flesh—by as much as 4.3 times—while the total content of flavonols varied by 2.1 times. In apple peel, on the contrary, the greatest variation was recorded for the total flavonol content (by 4.4 times), and the total flavan-3-ol content varied the least (by 1.8 times). A proper match of a cultivar and a rootstock can program a fruit tree to grow larger amounts of higher-quality, antioxidant-rich, and high-nutrition-value fruit.

Study of Phenolic Compounds of Prunus Domestica Presscake and Antimicrobial Activity of Its Extract

Prunus domestica - is one of the main fruit crops in Ukraine with a production of about 200 tons per year. In the world over then 2000 varieties of this plant are grown. In Ukraine, the following varieties of plums are zoned: Anna Shpet, Renklod Altana, Ugorka Stanley, Ugorka Italian, Ugorka Azhanska, and others. This plant is the valuable source of polysaccharides and phenolic compounds. That is why plum fruits are a part of many dietary supplements with laxative and anti-inflammatory activity in treating chronic constipation. In our previous research, we studied the chemical composition of alcohol extracts and polysaccharide complex of plum fruits. Following types of pharmacological activities: diuretic, membrane stabilizing, anticoagulant laxative, hepatoprotective, were confirmed in the analysed substances. In the article, we present the results of a study of the chemical composition of the plum presscake that remained after the plum juice was obtained. The composition of phenolic compounds was analyzed by using TLC, SF and HPLC methods. Antimicrobial activity of the alcohol extract of plum presscake was investigated by agar diffusion method. The results obtained indicate the possibility of complex processing of raw materials and obtaining new antimicrobial drugs from the presscake of plum juice production.

Detailed investigation of the composition and transformations of phenolic compounds in fresh and fermented Vaccinium floribundum berry extracts by high-resolution mass spectrometry and bioinformatics.

IntroductionBlueberries are known for their very high content of biologically active phenolic compounds; nonetheless, differently from the North American and European species of blueberries, Neotropical blueberries have not been extensively studied yet.ObjectivesIn the present paper, the phenolic composition of Vaccinium floribundum Kunth, which is endemic to the Andean regions and grows 1,600 to 4,500 meters above sea level, was investigated by ultra‐high‐performance liquid chromatography coupled to high‐resolution mass spectrometry (UHPLC‐HRMS). Native and fermented berries were compared in terms of phenolic composition as well as antioxidant activity, total phenolic content, and total anthocyanin content.Materials and Methods V. floribundum native and fermented berries were extracted and analyzed by UHPLC‐HRMS. The acquired datasets were processed by Compound Discoverer 3.1 using a dedicated data analysis workflow that was specifically set up for phenolic compound identification.ResultsIn total, 309 compounds were tentatively identified, including anthocyanins, flavonoids, phenolic acids, and proanthocyanidins. The molecular transformations of phenolic compounds during fermentation were comprehensively investigated for the first time, and by a customized data processing workflow, 13 quinones and quinone methides were tentatively identified in the fermented samples. Compared to other species of the genus Vaccinium, a peculiar phenolic profile is observed, with low abundance of highly methylated compounds.ConclusionAndean berries are a rich source of a wide variety of phenolic compounds. Untargeted MS analyses coupled to a dedicated data processing workflow allowed expanding the current knowledge on these berries, improving our understanding of the fate of phenolic compounds after fermentation.

A Simple Method for the Determination of Polyphenolic Compounds from Grapevine Leaves

Grapevine leaves are photosynthetically the most active green organs providing carbohydrates that are of utmost importance for the regular vine’s metabolism and growth. Moreover, leaves are the pioneers of fungal infections caused by B. cinerea, E. necator, and P. viticola. Plant response to these microorganisms mostly depends on the content and composition of phenolic compounds abundantly found in the leaf’s outer tissues (epidermis, cuticle, trichomes). In order to obtain a satisfactory quantity of phenolic compounds, an experiment was conducted towards optimizing a solid–liquid extraction method. Variables were as follows: the type of organic solvent, the sample weight, the extraction temperature, and the extraction time. The optimal conditions were obtained by applying the response surface methodology. Therefore, by using acetonitrile as the organic solvent, conducting a single-step extraction at the temperature of 48 °C during the time period of 2 h and 25 min with a solid-to-solvent ratio of 1:56 g mL−1 (178 mg of leaves powder and 10 mL of extraction solvent) the optimal content of phenolic compounds was obtained. This protocol is reliable, fast, and relatively easy to perform for the determination of the abovementioned compounds.

Impact of Storage Controlled Atmosphere on the Apple Phenolic Acids, Flavonoids, and Anthocyanins and Antioxidant Activity In Vitro.

Apples are seasonal fruits, and it is important to prepare them adequately for storage and ensure proper storage conditions. In this study, we used ten different apple cultivars: ‘Alva’, ‘Auksis’, ‘Connell Red’, ‘Cortland’, ‘Ligol’, ‘Lodel’, ‘Noris’, ‘Rubin’, ‘Sampion’, and ‘Spartan’. We studied the qualitative and quantitative composition of phenolic compounds in the apple and apple extracts antioxidants activity before placing them in the controlled atmosphere chambers and again at the end of the experiment, eight months later. Different concentrations of O2, CO2, and N2, constant temperature, relative humidity, and removal of endogenous ethylene were continually maintained. HPLC analysis showed that the highest amount of 2265.7 ± 152.5 µg/g of chlorogenic acid was found in apple samples of the ‘Auksis’ cultivar stored under variant IV conditions. Different concentrations of gas in the controlled atmosphere chambers caused changes in antioxidant activity in whole apple and apple peel extracts. In our study, we found that the antioxidant activity of apple extracts varied between samples of different apple cultivars and depended on the composition of the controlled atmosphere. Determining the optimal storage conditions is beneficial to providing the consumers with apples that have a known and minimally altered chemical composition of phenolic compounds and the strongest antioxidant activity, which determine the use of apples in the healthy food chain.

Development, Validation, and Application of the UPLC-DAD Methodology for the Evaluation of the Qualitative and Quantitative Composition of Phenolic Compounds in the Fruit of American Cranberry (Vaccinium macrocarpon Aiton).

Phenolic compounds in the fruit of American cranberry (Vaccinium macrocarpon Aiton) determine the antioxidant, anti-inflammatory, anticancer, and other biological effects. The berries are used in the production of medicinal preparations and food supplements, which highlights the importance of qualitative and quantitative analysis of phenolic compounds in cranberry fruit raw material. The aim of our study was to develop and validate an efficient, cost-effective, reproducible, and fast UPLC-DAD methodology for the evaluation of the qualitative and quantitative composition of phenolic compounds in raw material and preparations of American cranberry fruit. During the development of the methodology, chlorogenic acid and the following flavonols were identified in cranberry fruit samples: myricetin-3-galactoside, quercetin-3-galactoside, quercetin-3-glucoside, quercetin-3-α-L-arabinopyranoside, quercetin-3-α-L-arabinofuranoside, quercetin-3-rhamnoside, myricetin, and quercetin. The developed and optimized UPLC-DAD methodology was validated according to the guidelines of the International Council for Harmonization (ICH), evaluating the following parameters: range, specificity, linearity (R2 > 0.999), precision (%RSD < 2%), LOD (0.38–1.01 µg/mL), LOQ (0.54–3.06 µg/mL), and recovery (80–110%). The developed methodology was applied to evaluate the qualitative and quantitative composition of phenolic compounds in fruit samples of cranberry cultivars ‘Baifay’, ‘Bergman’, ‘Prolific’, and ‘Searles’, as well as ‘Bain-MC’ and ‘BL-12′ clones. In the tested samples, the majority (about 70%) of the identified flavonols were quercetin derivatives. The greatest amount of quercetin-3-galactoside (1035.35 ± 4.26 µg/g DW) was found in fruit samples of the ‘Searles’ cultivar, and the greatest amount of myricetin-3-galactoside (940.06 ± 24.91 µg/g DW) was detected in fruit samples of the ‘Woolman’ cultivar.

Berries and Leaves of Actinidia kolomikta (Rupr. & Maxim.) Maxim.: A Source of Phenolic Compounds.

Berries of Actinidia kolomikta (A. kolomikta) are known for high ascorbic acid content, but the diversity of phenolic compounds has been little studied. The present research aimed to investigate phenolic compounds and antioxidant activity in berries and leaves of twelve A. kolomikta cultivars. The UHPLC-ESI-MS/MS technique was used to determine differences among cultivars in the quantitative composition of individual phenolic compounds. Antioxidant activity was determined by DPPH• free radical scavenging and CUPRAC methods. In the present study, 13 phenolic compounds were detected in berries, whereas leaves contained 17 phenolic compounds. Flavonols were the primary class found in both berries and leaves; other identified phenolic compounds were flavan-3-ols, flavones and, phenolic acids; and dihydrochalcone phloridzin was identified in the leaves. The amount and variety of phenolic compounds in berries and leaves and antioxidant activity were found to be cultivar-dependent. The highest total content of phenolic compounds was found in the leaves of the cultivar ‘Aromatnaja’ and in the berries of the cultivar ‘VIR-2’. Results of this study have confirmed that berries and leaves of A. kolomikta could be a valuable raw material for both food and pharmaceutical industries.

Изменение спектра фенольных соединений циннии при избытке ионов меди в субстрате

Changes in the qualitative and quantitative composition of phenolic compounds in response to adverse environmental influences are actively studied, primarily in connection with the antioxidant function of these compounds. However, phenolic compounds such as phenylpropanoids are known to be involved in cell wall lignification. Lignin deposition is not only a natural process for the formation of secondary cell walls, but it is also often considered as a non-specific reaction of plants under stress conditions. However, changes in the content and composition of phenolic compounds during lignification in response to stressors have not been studied enough. Previously, we showed a decrease in the amount of phenolic compounds in response to the action of copper ions, but the question remained whether they are spent on the lignification process or on the quenching of reactive oxygen species. In this regard, the purpose of this work was to identify changes in the amount and composition of phenolic compounds of different polarity in the axial organs of zinnia when grown in a medium with an excess of copper ions. It was shown that under stress caused by copper ions (watering the substrate with a solution of 200 μM copper sulfate), in the roots of 40-day-old zinnia plants, the total content of phenolic compounds did not change significantly. At the same time, the content of fractions soluble in diethyl ether (hydroxycinnamic acids) and ethyl acetate (aglycones of flavonoids and coumarins) decreased, which may indicate the use of phenylpropanoids for additional synthesis of lignin, and flavonoids for antioxidant reactions under stress. In the hypocotyl, the content of hydroxycinnamic acids slightly increased, while in the first internode it did not change. The main part of phenolic compounds in all organs, both in control stressed plants, belonged to the fraction of glycosylated derivatives. At the same time, among this derivates the content of water-soluble phenols increased in the experimental plants in all organs. Thus, under the stress caused by copper ions, the composition and number of phenolic compounds changed.

Characterization of Feijoa sellowiana leaves based on volatile and phenolic compound compositions and antimicrobial properties

In the present research study, response surface methodology (RSM) was performed on the basis of total phenolic content of aqueous feijoa leaf extracts. The total phenolic content of experimental runs changed between 941.6 and 4347.5 mg GAE/L. HPLC-DAD analysis was performed to monitor the phenolic profile of feijoa leaf extract. Major phenolic compounds were determined as gallic acid, catechin, syringic acid, ellagic acid, chrysin, caffeic acid, p-coumaric acid, ferulic acid, and quercetin. Profile of volatile compounds was determined by using GC-MS technique and limonene, linalool and caryophyllene were detected as major volatile compounds. In addition, extracts from feijoa leaves showed antimicrobial activity against Gram-positive and Gram-negative bacteria and one yeast species. Taken together, all findings enhance our understanding of further use feijoa leaves as potential source for valuable bioactive compounds in various industrial products such as dietary supplements, food products (syrups, jams), and cosmetic products.