The article presents the results of the genealogical analysis of Ukrainian population of horses Orlov’s Trotter breed. The total number of the breed at the time of the survey was 653 horses, including 267 mares. The largest number of horses is in Zaporizhzhya stud no. 86 (29.6% mares). Analysis of the genealogical structure of the breed’s reproductive composition showed the advantage of stallions of three lines: Barchuk (branch of the Zapad, 28.1%), Pilot (18.8%), and Voin (15.6%). The mother composition mainly refers to the lines of Barchuk (branch of the Zapad, 30.7%), Pion (22.1%), Pilot (15.7%). The breeding stock of the Dibrivka stud is represented mainly by mares of the Pion line (41.2%), the Zaporozhsky stud — Barchuk line (a branch of the Zapad, 29.1%), Ispolnitielnyi (22.8%), and Pion (20.3%). In Lymarivka stud, most mares belong to the lines of Barchuk (branch of the Zapad, 35.5%), Pilot, Pion (25.8% each). Lozova stud is equipped with mares mainly of the Barchuk line (branch of the Zapad, 38.2%), Pilot (23.5%) and Veter (20.6%). The largest breeding reproducer of the “Komyshanske” private agrarian enterprise is equipped mainly with mares of the Barchuk line (branch of the Zapad, 51.2%), Pion (22.0%) and Pilot (17.1%). Analysis of the Ukrainian part of the Orlov Trotter breed population genealogical lines showed that in the presence of breeding and repair stallions, the greatest development was received by the Barchuk genealogical line, from which a new, more branched branch of the Zapad is separated, which 28.1% of stallions and 30.7% of mares belong to. The breeding core of this genealogical line is concentrated in all stud farms for breeding the Orlov Trotter breed (basic farms), as well as in breeding reproducers and individual horse owners. Thus, there is a prerequisite for testing the genealogical line of the West in the Ukrainian part of the population of the Orlov Trotter horse breed.