Composition Of Human Milk Research Articles

Influence of Diabetes during Pregnancy on Human Milk Composition.

Human milk (HM) is a unique nourishment believed to contain biological factors contributing to both short and long-term benefits. Considering that a mother’s own milk is often considered the first choice for nutrition of neonates, an aspect of increased interest is the possible effect of diabetes on the mammary gland and therefore on breast milk composition. This article aims to review the published literature on this topic, and to offer additional insights on the role of this disease on the composition of HM. This review was performed by searching the MEDLINE, EMBASE, CINHAL and Cochrane Library databases. A total of 50 articles were selected, focused specifically on one of the two types of diabetes: gestational diabetes mellitus (21 studies) and insulin-dependent diabetes mellitus (8 studies). Overall, the findings from the literature suggest that diabetes can alter the composition of HM. Nevertheless, the studies in this field are scarce, and the related protocols present some limitations, e.g., evaluating the variability of just a few specific milk biochemical markers in association with this syndrome.

Sexually Dimorphic Associations between Maternal Factors and Human Milk Hormonal Concentrations

While human milk composition is characterised by marked dynamicity, we are far from having a clear picture of what factors drive this variation. Hormones in human milk are known to vary according to specific maternal phenotypes, but limited evidence shows the infant also has a role in determining milk composition. The present study aimed to investigate the interplay between maternal and infant characteristics in relation to human milk hormonal profile. In total, 501 human milk samples from mothers recruited in the Finnish STEPS cohort study (Steps to the healthy development) were analysed. Pre-pregnancy and pregnancy maternal data, socioeconomic status and infant characteristics at birth were collated. Leptin, adiponectin, insulin-like growth factor-1 and cyclic Glycine-Proline in milk were measured. Multivariate analysis of variance (MANOVA) and linear regression were utilised for statistical analysis. Sex-specific interactions with maternal factors were observed, as the infant sex mediated associations between gestational diabetes and milk adiponectin (p = 0.031), birth-mode and total protein (p = 0.003), maternal education and insulin-like growth factor-1: cyclic Glycine-Proline ratio (p = 0.035). Our results suggest that changes in human milk composition are associated with interactions between maternal and infant characteristics and pathophysiological factors. Future work should expand on these findings and further explore the link between hormonal profiles in human milk and infant outcomes.

Microbiota Innovative Management Modalities in Mastering a Healthy Gut … Feeding Update …!

Introduction: Our mini review aims to state the evidence-based effect of the implementation of newborn infant nutrition with prebiotics and probiotics has been demonstrated in changing microflora composition toward the desired breast-feeding pattern and stimulating immune response Considerable efforts have been made to mimic the composition of human milk by the addition to formula feeding of living bacteria (probiotics), non-digestible fibers, nucleotides and oligosaccharides (prebiotics), and bovine lactoferrin in order to induce a breast-fed-similar microbiota colonization in formula-fed infants, with the final aim to stimulate the maturation and proper function of the immune system Several studies performed in the past decades have clearly demonstrated the complexity of gut microbiota composition and the modulatory effect played by several endogenous and exogenous factors on it. Type of feeding in the first months of life appears as one of the most important determinants of the child and adult well-being, and its protective action seems to rely mainly on its ability to modulate intestinal microflora composition at early stages of life. Diet has a dominant role over other possible variables such ethnicity, sanitation, hygiene, geography, and climate, in shaping the gut microbiota. In recent years, the implementation of milk formula with prebiotics, probiotics, and lactoferrin has been demonstrated to change newborns’ microflora composition toward breast-feeding pattern and stimulate immune response Conclusions: The aim of this Mini Review is to elucidate the specific immunologic role of the human milk-associated microbiota and its impact on the newborn’s health and life, highlighting the importance to properly study the biological interactions in a bacterial population and between the microbiota and the host. This mini review discusses the composition of human milk and its biological benefit for infants. Additionally, we also discuss how these beneficial effects can be mimicked if breastfeeding is not possible. And to highlight the specific and fundamental role of human milk-associated bacteria in modulating and influencing the newborns’ immune system during their life.

ОХРАНА И ПОДДЕРЖКА ГРУДНОГО ВСКАРМЛИВАНИЯ ДЛЯ ДОСТИЖЕНИЯ ЦЕЛЕЙ УСТОЙЧИВОГО РАЗВИТИЯ. АНАЛИТИЧЕСКИЙ ОБЗОР

Significance. World Health Organization correlates breastfeeding with at least eight out of the 17 UN Sustainable Development Goals. However, in the Russian Federation breastfeeding issues are not reflected in any of the Federal programs of the national projects “Demography” and “Health Care”. Purpose of the study: to scientifically substantiate the need for including measures to encourage and maintain breastfeeding in the Russian national programs related to the achievement of the UN Sustainable Development Goals, based on the analysis of modern literature. Material and methods. The study included sources available from the English-language database PubMed. A total of 57 sources were selected out of 317 findings by keywords “human milk composition and bioactivity”, “breastfeeding and Sustainable Development Goals”, “breastfeeding short-term and long-term benefits” and “breastfeeding and potential lives saved”. Results. The study revealed unique biologically active components of the human milk and mechanisms of their impact on prevention of infectious and non-communicable diseases as well as programming of health in adult life. Breastfeeding has a positive impact on mothers’ and children’ health. The increased rate of breastfeeding could globally prevent 823,000 or 13.8% of deaths among children under 2 yearly and 22216 deaths from breast cancer among women. Lack of breastfeeding is associated with lower intelligence and higher economic costs adding up to about $ 302 billion annually, or 0.49% of the world's gross national income. There are unfavorable trends in prevalence and duration of breastfeeding worldwide. The healthcare has a leading role to play in implementing measures to encourage and maintain breastfeeding. Conclusion. It is necessary to incorporate measures to encourage and maintain breastfeeding in the Russian national programs related to the achievement of the UN Sustainable Development Goals. Implementation and further development of the WHO/UNICEF “Baby-Friendly Hospital Initiative” in the Russian Federation can become the cornerstone in increasing the level of breastfeeding with the participation of the health sector. Scope of application. Executive and health authorities at all levels, mother and child health facilities, national programs

Мальтодекстрин – ингредиент для производства продуктов детского питания. Исследование состава и свойств

The main principles of development of adapted infant milk formulas are approximation to the composition and properties of human milk and correspondence to the specific features of infantile digestion and metabolism. Of no least significance is adjustment of the carbohydrate component. Partial replacement of lactose by maltodextrin allows to decrease the osmolality of milk formulas. Maltodextrin is well utilized in the intestines, has a positive effect on its microbiota. Therefore, in modern infant adapted formulas lactose is partly replaced by maltodextrin. Infant formulas are manufactured using maltodextrin with the dextrose equivalent from 18 to 22. The study was aimed at investigating the possibility of using maltodextrin manufactured in Russia in specialised food products for infant nutrition. Three samples of maltodextrin were selected for the study: 2 were manufactured in Russia and 1 was imported. The samples were tested in the following order: study of comparative characteristics of maltodextrins; experimental pre-production of adapted milk formulas with maltodextrin samples, and examination of the quality and safety characteristics and the technological benefits of its use. The research work and its results confirmed the possibility of using maltodextrin made in Russia for manufacture of adapted products for child nutrition to optimise the carbohydrate composition of the final product. Key words: dextrose equivalent, child nutrition, maltodextrin, solubility

Nutritional composition of donor human milk according to lactation period

Introduction: when own mother´s breast milk is unavailable, donor human milk is the best option to guarantee the nutritional requirements of preterm or ill newborns. Objectives: to analyze the macronutrient composition of donor breast milk and its evolution over lactation periods. Material and methods: we retrospectively analyzed the nutritional composition of 822.423 L of donor breast milk from 160 donors at the human milk bank of a third-level hospital between February 1, 2017 and December 31, 2019. Results: lipid and carbohydrate composition remains constant throughout lactation. However, protein content decreases initially to increase again starting in the second year of lactation. Conclusions: donor human milk banks should be aware of the variations in macronutrient composition donor human milk shows in order to optimize nutrition for preterm newborns.

The Effect of Holder Pasteurization and Storage on Macronutrients in Donor Human Milk

Objective: Milk delivered to human milk banks should be pasteurized and stored at -20°C in order to inactivate any microbial agents that may be present. We aimed to quantify the changes in the macronutrient composition of donor human milk (DHM) that underwent Holder pasteurization (HoP) and subsequent storage. Methods: A total of 54 breast milk samples from 26 healthy lactating mothers were collected at different time points after delivery, at intervals ranging from 1-6 months. We measured the carbohydrate, protein, fat, and energy content before and after HoP. After HoP, DHM was stored at -20°C, and the nutrients were measured at 1, 2, 4, 8, 12, 16, and 20 weeks after storage commenced. Results: The difference in time between expression of milk and pasteurization did not affect the concentration of macronutrients. However, the protein, fat, and energy contents decreased significantly during HoP. The mean values of the protein, fat, and energy contents in DHM pre-HoP were 1.2 g/dL, 3.7 g/dL, and 72.1 kcal/dL, compared with post-HoP values of 1.0 g/dL, 3.1 g/dL, and 65.2 kcal/dL, respectively. Although HoP reduced the protein, fat, and energy contents of donor milk by 16.7%, 16.2%, and 9.6%, respectively, the carbohydrate content was not reduced. Moreover, there was a significant decrease in the content of all the analyzed macronutrients following storage for up to 20 weeks. Conclusion: The post-HoP storage period affected nutrients, with several human milk components decreasing in content after HoP. As such, DHM after HoP may need fortification to ensure normal infant growth.

Perspective: Human Milk Oligosaccharides: Fuel for Childhood Obesity Prevention?

Obesity begins early but has lifelong consequences for health and well-being. Breastfeeding is thought to be preventive against obesity, but the extent and cause of this association are not well understood. Human milk oligosaccharides (HMOs) are abundant in human milk and not present in commercially available infant formula. These complex sugars are thought to contribute to the development of the infant gut microbiome and immune system. Recently, they have been investigated as a potential link between breastfeeding and lower obesity risk. So far, only a few human studies have examined HMO composition of human milk in association with the infant's concurrent anthropometry or subsequent growth in infancy, with conflicting results. However, HMOs have been shown to modulate the gut microbiome profile by selectively promoting the growth of specific bacteria, such as bifidobacteria. Moreover, there are differences in the gut microbiome of lean and obese humans, and there is some evidence that the early composition of the gut microbiome can predict later obesity. Although it seems that HMOs might have a role in infant growth and adiposity, there is not enough consistent evidence to understand their potential role in obesity prevention. More data, particularly from large or longitudinal studies, are needed to clarify the functions of HMOs and other breast-milk components in determining long-term health.

The safety study of the fat component in adapted infant formula

The growth and harmonious development of an infant depends on nutrition provided by natural or artificial feeding. Accordingly the adaptation of the composition of adapted infant formula to the composition of human milk is carried out with all major nutrients. The fat component is one of the key components of an infant’s diet and its’ important aspects are biological effectiveness and safety which changes during processing, storage, transportation and subsequent storage after opening the package. Data on pathological changes in the body with consumption of oxidized fats are known. The aim of the paper is to study some indicators of safety and quality of the fat component of adapted infant formula provided by various manufacturers. As the objects of study we selected one of the most popular adapted infant formula in Russia (in various price categories) under the code names: “IS” (Denmark), “IM”, “IN”, “IL” (Russian Federation), “IX “(Germany). It was found that the quality composition of the fat component of dry infant formula corresponds to the one indicated on the package. However, no one of the test samples according to the averaged composition of the prevailing fatty acids, is fully identical to human milk. The normative documentation of the Customs Union (TR TS 021/2011, TP TS 024/2011, TP TS 033/2013) only regulates organoleptic analysis of the quality indicators of adapted infant formula and from the safety parameters, only finding the peroxide value, which characterizes the accumulation of primary fat oxidation products. It was found that the peroxide numbers of the studied infant formula do not exceed the regulated values. At the same time almost all milk nutrition made from dry infant formula has unsatisfactory organoleptic characteristics. Defects of taste and aroma can be associated with the accumulation of a significant amount of secondary products of fat oxidation, aldehydes particularly in the adapted infant formula. These conclusions are confirmed by the results of determination of the anisidine number in the adapted infant formula, as well as by the accumulation of 0.3-1.0% of highly polar compounds insoluble in petroleum ether (CIPE) and 25-3 mmol/kg of epoxides which lead to decrease of white blood cells and a change in blood formula at a biological animal test. The data obtained indicate the need for assessment of the technology for the production, packaging and storage of adapted infant formula as well as the feasibility of amending the regulatory documents of the Russian Federation and in the future, the regulatory documents of the Customs Union with the aim of further monitoring the safety of the fat component of adapted infant formula.

Subclinical Mastitis in a European Multicenter Cohort: Prevalence, Impact on Human Milk (HM) Composition, and Association with Infant HM Intake and Growth.

Background: Subclinical mastitis (SCM) is an inflammatory condition of the mammary gland. We examined the effects of SCM on human milk (HM) composition, infant growth, and HM intake in a mother–infant cohort from seven European countries. Methods: HM samples were obtained from 305 mothers at 2, 17, 30, 60, 90, and 120 days postpartum. SCM status was assessed using HM Sodium (Na): Potassium (K) ratio >0.6. Levels of different macro- and micronutrients were analyzed in HM. Results: SCM prevalence in the first month of lactation was 35.4%. Mean gestational age at delivery was lower and birth by C-section higher in SCM mothers (p ≤ 0.001). HM concentrations of lactose, DHA, linolenic acid, calcium, and phosphorous (p < 0.05 for all) was lower, while total protein, alpha-lactalbumin, lactoferrin, albumin, arachidonic acid to DHA ratio, n-6 to n-3 ratio and minerals (iron, selenium, manganese, zinc, and copper) were higher (p < 0.001 for all) in mothers with SCM. There were no differences in infant growth and HM intake between non-SCM and SCM groups. Conclusion: We document, for the first time, in a large European standardized and longitudinal study, a high prevalence of SCM in early lactation and demonstrate that SCM is associated with significant changes in the macro- and micronutrient composition of HM. Future studies exploring the relation of SCM with breastfeeding behaviors and developmental outcomes are warranted.

Temporal Changes of Phospholipids Fatty Acids and Cholesterol in Breast Milk and Relationship with Diet

AbstractPhospholipids (PLs) and cholesterol in human milk (HM) are affected by lactation, and differential lipids are closely related to maternal diet. The contents of PLs and cholesterol in Chinese HM are quantified by gas chromatography and high performance liquid chromatography, respectively, and the relationship between differential lipids and the maternal diet is obtained by Pearson. The result shows that SFA, MUFA, and polyunsaturated fatty acid (PUFA) are not affected by lactation and geography for total fatty acids, but almost all sn‐2 fatty acids are influenced by geography and remain unchanged during lactation. Most SFAs show absolute sn‐2 selectivity and the majority of MUFAs and PUFAs are esterified at the sn‐1 position. Cholesterol (13.8–22.6 mg per 100 g milk) and 25‐hydroxycholesterol (0.45–1.01 mg per 100 g milk) increase significantly and remain constant during lactation, respectively, and they are affected by regions. In addition, the differential lipids (22:1n‐9, C9:0, trans‐PUFA, 22:4n‐6, etc.) of PLs are closely related to the maternal diet. PLs and cholesterol content differ from western research and infant formula, which will help to design an infant formula that is more suitable for Chinese babies in the future.Practical Application: Compared with PLs and cholesterol in western countries and infant formula, the specificity of Chinese HM can more accurately target the development of formulas suitable for the growth of Chinese infants. At the same time, according to the influence of the motherʼs diet on the composition of HM, it is more reasonable to guide the diet of the mother.

A Review of the Immunomodulating Components of Maternal Breast Milk and Protection Against Necrotizing Enterocolitis.

Breast milk contains immunomodulating components that are beneficial to newborns during maturation of their immune system. Human breast milk composition is influenced by an infant’s gestational and chronological age, lactation stage, and the mother and infant’s health status. Major immunologic components in human milk, such as secretory immunoglobulin A (IgA) and growth factors, have a known role in regulating gut barrier integrity and microbial colonization, which therefore protect against the development of a life-threatening gastrointestinal illness affecting newborn infants called necrotizing enterocolitis (NEC). Breast milk is a known protective factor in the prevention of NEC when compared with feeding with commercial formula. Breast milk supplements infants with human milk oligosaccharides, leukocytes, cytokines, nitric oxide, and growth factors that attenuate inflammatory responses and provide immunological defenses to reduce the incidence of NEC. This article aims to review the variety of immunomodulating components in breast milk that protect the infant from the development of NEC.

Human Milk Omega-3 Fatty Acid Composition is Associated with Infant Temperament.

There is growing evidence that omega-3 (n-3) polyunsaturated fatty-acids (PUFAs) are important for the brain development in childhood and are necessary for an optimal health in adults. However, there have been no studies examining how the n-3 PUFA composition of human milk influences infant behavior or temperament. To fill this knowledge gap, 52 breastfeeding mothers provided milk samples at 3 months postpartum and completed the Infant Behavior Questionnaire (IBQ-R), a widely used parent-report measure of infant temperament. Milk was assessed for n-3 PUFAs and omega-6 (n-6) PUFAs using gas-liquid chromatography. The total fat and the ratio of n-6/n-3 fatty acids in milk were also examined. Linear regression models revealed that infants whose mothers’ milk was richer in n-3 PUFAs had lower scores on the negative affectivity domain of the IBQ-R, a component of temperament associated with a risk for internalizing disorders later in life. These associations remained statistically significant after considering covariates, including maternal age, marital status, and infant birth weight. The n-6 PUFAs, n-6/n-3 ratio, and total fat of milk were not associated with infant temperament. These results suggest that mothers may have the ability to shape the behavior of their offspring by adjusting the n-3 PUFA composition of their milk.

Putting the "M" in Breastfeeding Medicine.

Putting the "M" in Breastfeeding Medicine.

Comparative lipidomics analysis of human, bovine and caprine milk by UHPLC-Q-TOF-MS

Although infant formula represents an important human milk substitute, it has been shown to contain many different lipid structures compared to human milk. In this study, the lipidome and fatty acid composition of human, bovine and caprine milk were analyzed and compared. A total of 13 lipid classes (including TG, DG, SM, PC, Cer, HexCer, Hex2Cer, PE, PG, PS, PI, PA and CL) were analyzed. Human milk was richer in TG containing LA, SM containing ULCFA and PLs containing ARA, DHA and DGLA. Moreover, there were no differential DG and SM species between human and caprine milk. A total of 215 and 147 lipids were identified as potential biomarkers that could be used to further analyze differences in the biological properties of human, bovine and caprine milk. This comprehensive analysis will hopefully help in the design of infant formula more suitable for Chinese babies.

Lipidomics of Brain Tissues in Rats Fed Human Milk from Chinese Mothers or Commercial Infant Formula.

Holistic benefits of human milk to infants, particularly brain development and cognitive behavior, have stipulated that infant formula be tailored in composition like human milk. However, the composition of human milk, especially lipids, and their effects on brain development is complex and not fully elucidated. We evaluated brain lipidome profiles in weanling rats fed human milk or infant formula using non-targeted UHPLC-MS techniques. We also compared the lipid composition of human milk and infant formula using conventional GC-FID and HPLC-ELSD techniques. The sphingomyelin class of lipids was significantly higher in brains of rats fed human milk. Lipid species mainly comprising saturated or mono-unsaturated C18 fatty acids contributed significantly higher percentages to their respective classes in human milk compared to infant formula fed samples. In contrast, PUFAs contributed significantly higher percentages in brains of formula fed samples. Differences between human milk and formula lipids included minor fatty acids such as C8:0 and C12:0, which were higher in formula, and C16:1 and C18:1 n11, which were higher in human milk. Formula also contained higher levels of low- to medium-carbon triacylglycerols, whereas human milk had higher levels of high-carbon triacylglycerols. All phospholipid classes, and ceramides, were higher in formula. We show that brain lipid composition differs in weanling rats fed human milk or infant formula, but dietary lipid compositions do not necessarily manifest in the brain lipidome.

Effect of lactation stages and dietary intake on the fatty acid composition of human milk (A study in northeast China)

The correlation among lactation stages (until 30 days), lactation dietary intake and fatty acid (FA) composition in human milk (n = 202) was evaluated. The total saturated fatty acids (SFAs) and total monounsaturated fatty acids (MUFAs) remained stable over lactation progression, but medium-chain SFAs increased during the lactation stages. The n-6 polyunsaturated fatty acids were in highest concentrations in mature milk owing to the highest levels of C18:3n-6 and C18:2n-6 (0.18% and 46.06% respectively). Linoleic acid and α-linolenic acid (ALA) intakes were within the recommended levels. Linear regression analysis indicated that the intake of soybean oil and dietary ALA intake affected the n-6 PUFA content in colostrum and transitional milk positively. The effect of dietary intake on FA composition in human milk decreased during the first 30 days and nutritional regulation in the early stage of lactation should be highlighted.

Selected elements and fatty acid composition in human milk as indicators of seafood dietary habits

The maternal diet and living environment can affect levels of chemical elements and fatty acid (FA) composition and their stable isotopes (δ13CFA) in human milk. Information obtained from questionnaires is frequently imprecise, thus limiting proper associations between external and internal exposures as well as health effects. In this study, we focused on seafood as a source of potentially toxic and essential elements and nutritional FAs. Concentrations of selected elements in human milk (As, Cd, Cu, Mn, Pb, Se and Zn) were determined using inductively coupled plasma mass spectrometry (ICP-MS) and Hg using cold vapour atomic-absorption spectrometry (CV-AAS). The identification and quantification of FAs in maternal milk were performed by an in-situ trans-esterification method (FAMEs), and the characterization of FAMEs was performed by gas chromatography with a flame ionisation detector (GC-FID). δ13CFA was determined by gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). Seventy-four lactating Slovenian women from the coastal area of Koper (KP), with more frequent consumption of seafood, and the inland area of Pomurje (MS), with less frequent seafood consumption, were included in this study. Along with basic statistical analyses, data mining approaches (classification and clustering) were applied to investigate whether FA composition and δ13CFA could improve the information regarding dietary sources of potentially toxic elements. As and Hg levels in milk were found to be statistically higher in populations from KP than in those from MS, and 71% of individual FAs and 30% of individual δ13CFA values in milk differed statistically between the studied areas. In 19 cases, the levels of FAs in milk were higher in KP than in MS; these FAs include C20:5ω3 and C22:6ω3/C24:1ω9, which are typically contained in fish. In 16 cases, the mean percentage of FAs was higher in MS than in KP; these FAs include the PUFAs C18:2ω6, C18:3ω3, and C20:4ω6 which are important for human and infant growth. The difference in δ13C levels of C10:0, C12:0, C14:0, C16:1, C16:0, C18:1ω9c, C22:6ω3, and δ13C 18:0–16:0 in the study groups was statistically significant. In all seven cases where δ13C of FA significantly differed between KP and MS, δ13C was higher in KP, indicating a higher proportion of a marine-based diet. The data mining approaches confirmed that the percentage of selected FAs (iC17:0, C4:0, C18:2ω6t, aC17:0, CLA, and C22:4ω6) and δ13CFA of C18:1ω9c in human milk could be used to distinguish between high and low frequency of fresh seafood consumption.

Variations of Human Milk Compositions as per Prakriti (Psychosomatic Constitution): A Pilot Study

Introduction: Mother’s milk is considered to be the supreme food for neonates, infants and toddler. It is also regarded as the finest gift form a mother to her baby. The individual characteristic of a person enumerating external and internal body features is known as Prakriti. It is the sense of personality or constitution or temperament of an individual. The aim of the present study was to assess “variations of human milk composition as per Prakriti”. Materials and Methods: Total 64 lactating mothers of 20-35 years age group, not suffering from any acute or chronic illness and who were on similar diet regardless of their mode of delivery were registered and included in the study while lactating mothers not falling in this age group or who were taking any specific drug for the illness or suffering from any disease including- moderate (9.97-7 g/dl) to severe (6.94-4 g/dl) anemia were excluded from the study. Result: On applying One Way ANOVA, the mean values of fat percentage in milk varied significantly as per Prakriti (p = 0.0287). On applying Post Hoc Test the significant pairs were for Vata vs. Pitta (<0.05) and Vata vs Kapha (<0.05) Prakriti . In all other parameters of milk, no significant variation was found. Conclusion: We can conclude that the composition of mother’s milk varies as per Prakriti .

Hypoallergenic and Low-Protein Ready-to-Feed (RTF) Infant Formula by High Pressure Pasteurization: A Novel Product.

Infant milk formula (IMF) is designed to mimic the composition of human milk (9–11 g protein/L); however, the standard protein content of IMF (15 g/L) is still a matter of controversy. In contrast to breastfed infants, excessive protein in IMF is associated with overweight and symptoms of metabolic syndrome in formula-fed infants. Moreover, the beta-lactoglobulin (β-Lg) content in cow milk is 3–4 g/L, whereas it is not present in human milk. It is considered to be a major reason for cow milk allergy in infants. In this respect, to modify protein composition, increasing the ratio of alpha-lactalbumin (α-Lac) to β-Lg would be a pragmatic approach to develop a hypoallergenic IMF with low protein content. Such a formula would ensure the necessary balance of essential amino acids, as 123 and 162 amino acid residues are available in α-Lac and β-Lg, respectively. Hence, in this study, a pasteurized form of hypoallergenic and low-protein ready-to-feed (RTF) formula, a new product, is developed to retain heat-sensitive bioactives and other components. Therefore, the effects of high pressure processing (HPP) under 300–600 MPa at approximately 20–40 °C and HTST pasteurization (72 °C for 15 and 30 s) were investigated and compared. The highest ratio of α-Lac to β-Lg was achieved after HPP (600 MPa for 5 min applied at 40.4 °C), which potentially explains the synergistic effect of HPP and heat on substantial denaturation of β-Lg, with significant retention of α-Lac in reconstituted IMF. Industrial relevance: This investigation showed the potential production of a pasteurized RTF formula, a niche product, with a reduced amount of allergenic β-Lg.