Soybean Components Research Articles

Current perspectives on the beneficial effects of soybean isoflavones and their metabolites on plants.

Soybeans have traditionally been a staple part of the human diet being highly rich in protein and lipid content. In an addition to the high nutritional components, soybeans have several functional components, like isoflavones, saponins, lecithin, and oligosaccharides. Soybeans emerge as a healthy functional food option. Isoflavones are most notable functional component of soybeans, exhibiting antioxidant activity while preventing plant-related diseases (e.g., antimicrobial and antiherbivore activities) and having positive effects on the life quality of plants. Isoflavones are thus sometimes referred to as phytochemicals. The latest research trends evince substantial interest in the biological efficacy of isoflavones in the human body as well as in plants and their related mechanisms. However, there is little information on the relationship between isoflavones and plants than beneficial human effects. This review discusses what is known about the physiological communication (transport and secretion) between isoflavones and plants, especially in soybeans.

Semantic segmentation-based mechanized harvesting soybean quality detection

Crushing rate and impurity rate are important quality indicators of mechanically harvested soybeans. Intelligent quality detection of mechanically harvested soybeans based on machine vision is of great significance to evaluate soybean quality accurately and rapidly. This study proposes an improved U-Net method for identifying intact soybean grains, crushing soybean grains, and impurities. Based on the accurate identification of soybean components and using the quantitative model of soybean crushing rate and impurity rate, the quality of soybean samples can be detected in real-time. To this end, a soybean quality inspection system is designed to realize the dynamic collection and detection of soybean samples. The test results show that the comprehensive evaluation index values of the improved U-Net segmentation algorithm in identifying intact soybean grains, crushing soybean grains, and impurities are 93.04%, 89.40%, and 96.49%, respectively. Compared with the traditional U-Net model, the performance of the indicators is improved by 3.23%, 0.17% and 0.72%, respectively. Compared with manual detection, the maximum absolute error of the crushing rate detection of the soybean quality detection system is 0.57%, and the maximum absolute error of the impurity rate detection is 0.69%. The proposed soybean quality inspection system can be used as an effective tool for real-time online inspection of soybean quality.

Soybean Varieties Respond to the Shade of Teak Trees

Background: Light is one of the environmental factors that greatly affect the growth and yield of crops in general, including soybean plants. The purpose of this study was to determine the effect of shade on yield and yield components of soybean varieties. Methods: The research method was a randomized complete block design (RCBD) with three replications. The first factor was the kind of variety which consisted of two levels, i.e., Dena I and Anjasmara. The second factor was the level of shading, which consisted of four levels, i.e., 0, 10-20, 20-30 and 30-40%. Result: The results showed that shade decreased the number of filled pods, dry pod weight per plant, 100 seeds weight and seed yield ha-1. As the shade increases, the lower the yield and component of soybean yields increases. The decrease in seed yield ha-1 of Dena I variety at 10-20, 20-30 and 30-40% shade was 22, 14 and 50%, respectively, while the Anjasmara variety was 9, 49 and 59%. Shade decreases yields and yield components of Dena I and Anjasmara varieties. In the higher level of shade, the yield reduction will increase.

Poultry manure effects on yield and some agronomic components of Soybean (Glycine max L.) under Khost agro-ecological conditions, Afghanistan

Soybean [Glycine max (L.) Merrill], which has the highest protein content of all food crops, is the world’s leading source of protein and oil. Soybeans are commonly consumed by humans in the form of soymilk, soy protein, tofu, infant formula, miso, natto, soy flour, and soy sauce. They are a popular protein-rich food source in most Asian countries. Worldwide, approximately 85% of soybean's produce has been processed into soy food. While in developing countries like Afghanistan, limited farmers used inorganic fertilizers in soybean fields through the high cost, marketing problems and poor economic conditions. If we have alternative resources for soil fertility like organic manure, this problem can somewhat be solved. The objective of this study was to test the effects of different levels of poultry manure on the yield and yield components of the soybean crop. The field experiment was conducted in 2019 in Almara Village of Nadar Shah Kot District of Khost Province. Soybean variety LD04-13265 was grown with four levels of manure (0, 1, 2, and 4 tons ha-1) using Randomized Complete Block Design with three replicates. Results revealed that manure invariably influenced most parameters under study. The highest grain yield (1212.95 kg ha-1) was obtained with 4 tons ha-1 (T3) followed by T2 and T1 (1145.16 and 1138.24, respectively). Poultry manure also had positive effects on the agronomic characters. Plant height, number of branches, pods plant-1, seed pod-1 were among the most affected. Correlation analysis revealed significant positive correlations among the grain yield and yield components but non correlation was found for 100 seed weight with other observed parameters. As per the results of this research, 4 tons of poultry manure is the optimum amount to be used for soybean production.

Herbicides in soybean development: productive aspects and physiological quality of seeds

The objective was to evaluate the production components of soybean and the physiological quality of seeds according to the application of subdoses of herbicides at the reproductive stage of healthy plants. The treatments consisted of the application of glyphosate (122.5 g acid equivalent [ae] ha-1), chlorimuron (1.75 g active ingredient [ai] ha-1), 2,4-D (134 g ae ha-1), fomesafen (60 g ai ha-1), glufosinate (55 g ai ha-1), paraquat (35 g ai ha-1), and control (no application). The chlorophyll index, agronomic performance, and physiological quality of seeds were evaluated. Glyphosate, chlorimuron, fomesafen, or glufosinate resulted in an increase in soybean plant height. Chlorimuron increased yield compared to the control. Paraquat or glufosinate, despite the injury, did not reduce yield, but the application of these herbicides is not recommended to stimulate plant development, given the potential for injury. The 2,4-D was the only one that caused a reduction in the physiological quality of soybean seeds.

Fungicide Spraying Programs Reducing Asian Soybean Rust Impact on Soybean Yield Components

Soybean is one of the leading agricultural commodities, and Brazil is currently the largest producer globally. Despite this, fungal diseases as Asian Soybean Rust (ASR) are among the primary limiters of high crop yields in Brazilian fields. Losses caused by the biotrophic fungus Phakopsora pachyrhizi can reach up to 90%, depending on weather conditions, and several components can be affected during soybean growth and reproduction. Here, we assessed fungicide spraying programs to ASR control, aiming to reduce the losses on soybean components. The experiments were performed under field conditions during the harvest season 2014/2015. The evaluated variables were soybean leaf area index, ASR severity, yield components, dry mass grain accumulation, protein, and oil content. The yield components assessed were the number of pods per plant, seeds per pod, seeds per plant, and thousand-grain weight. The disease severity gradient was generated using seven fungicide spraying programs, differing in time, number, and type of fungicides. Three fungicide programs that included applications in the soybean vegetative and reproductive stages were more efficient. These programs resulted in the lower area under the disease progress curve (AUDPC), greater leaf area duration (LAD), and health leaf area duration (HAD) than the untreated soybean. The ASR infection in soybean resulted in reduced LAD and HAD, and as a consequence, interfered negatively with dry matter accumulation, yield components, and grain yield.

Gene mining of 100‐grain weight and the number of four‐seed pods in soybean (Glycine max)

AbstractThe number of four‐seed pods and 100‐grain weight are important yield components in soybean. Typically, they are negatively correlated traits. Generally, soybean varieties with a high 100‐grain weight and a higher number of three‐ or four‐seed pods are more likely to obtain a high yield. It is difficult to select for double‐excellent traits that meet the needs of farmers and the market using conventional breeding methods. The purpose of this study was to mine genes associated with the number of four‐seed pods and 100‐grain weight in soybean. Whole‐transcriptome sequencing was performed on four specific chromosome segment substitution lines (HWMN, HWFN, LWMN and LWFN) combined with the distribution of blocks imported from wild soybean DNA fragments and gene annotation. The material was sampled in each of the eight development stages. Among them, globular embryo formation, heart embryo formation, and cotyledon primordium formation were used to mine differentially expressed genes (DEGs). A total of 3792 DEGs were identified, and 25 expression patterns were obtained by the K‐means rapid clustering method. GO enrichment analysis of DEGs was performed by Agrigo, and a total of 43 GO terms were enriched. Through annotation analysis, 126 DEGs associated with seed size and number were obtained. Combined with analysis of the introduced DNA fragments of wild soybean ZYD00006, 19 genes that eventually aligned on those blocks were obtained. Six representative genes were selected as candidate genes, namely, Glyma.07G218200, Glyma.05G162100, Glyma.19G240800, Glyma.04G180300, Glyma.05G214400 and Glyma.12G146700. They may regulate the formation of 100‐grain weight and the number of four‐seed pods, which will provide reference information for the genetic improvement of agronomic traits, such as seed number per pod and 100‐grain weight of soybean, in the future and will enrich the theoretical basis of high yield in soybean.

Results of the study of the weed-field flora of Primorsky Territory in 2016-2020

The results of the study of the weed component of soybean, early grain crops and maize agrocenoses in Primorsky Territory are presented. In total, 111 species of weeds belonging to 35 families were found during the period from 2016 to 2020. Compared with the results of crop surveys conducted in 2006-2015, the total number of species detected has increased by 23. The largest number of species belongs to the families Asteraceae (24), Poaceae (15), Polygonaceae (11), Fabaceae (9), Brassicaceae (8), Caryophyllaceae (7) and Lamiaceae (5). The remaining 26 families were represented by 1-3 species each. For the first time weeds-representatives of the families Scrophulariaceae, Violaceae, Lythraceae, Onagraceae, Asclepiadaceae and Boraginaceae have been discovered. The floristic composition of soybean agrocenoses was the highest with 108 weed species from 31 families. In cereal and maize crops, weed diversity was less significant, with 75 species in 22 families and 72 species in 25 families, respectively. Weed vegetation in all the above crops is represented by 62 species of 19 families. The main weed species that grew on 97-99% of the surveyed territory with a sufficiently high average density of standing (21-61pcs/m2) were Asian copperleaf, cockspur grass and common ragweed. Also, more than half of the surveyed crops of soybeans, cereals and corn were hairy cupgrass, perennial sow thistle, common couch-grass, common lamb’s quarters, wormwood species, field horsetail, yellow thistle, dayflower and yellow foxtail. Practical measures to protect against weeds on the Primorsky Territory should be primarily aimed at controlling these species.

Nutritional quality of different potassium efficiency types of vegetable soybean as affected by potassium nutrition

Abstract Pot experiments were conducted in 2017, 2019, and 2020 to examine the effects of potassium nutrition on the nutritional components of vegetable soybeans with different K efficiency at immature and mature stages. Two vegetable soybean varieties with higher K efficiency and two varieties with lower K efficiency were studied in the low available K soil under the conditions of no K and normal K fertilization. The results indicated that almost all nutritional components in vegetable soybean were affected by K, genotypes, interannual differences, and their interactions. In general, no K fertilization increased protein and amino acid concentrations but decreased oil, soluble sugar, sucrose, K, Mg, and Fe concentrations in immature and mature vegetable soybean. The sensitivity of nutritional components to K nutrition differed among varieties. For instance, K high-efficiency varieties generally exhibited higher protein and amino acid concentrations without K application. K high-efficiency vegetable soybeans are low-K tolerance varieties to isoflavones. The results of this study provide insights for high yield and quality vegetable soybean breeding against soil K deficiency.

Enlist volunteer corn affects the crop development and seed quality of Enlist soybean

EnlistTM technology is a new trait on soybean and corn conferring tolerance to auxinic-type (2,4-D choline) and graminicides (aryloxyphenoxypropionates – FOPs) herbicides. However, the occurrence of volunteer corn tolerant to 2,4-D and FOPs can become a significant weed on soybean cropping systems, affecting the crop yield and reducing seed quality. This research aimed to evaluate herbicides to EnlistTM volunteer corn control and their effects on plant development, crop yield, and physiological seed quality of EnlistTM soybean. Field experiment was performed in a complete randomized block experimental design with four replicates, using a density of 10 plants·m-2 of EnlistTM volunteer corn. Treatments consisted of herbicides applied at 3-4-leaves stage of volunteer corn with haloxyfop-P-methyl, clethodim, pinoxaden, cloransulam-methyl and imazethapyr regarding to a control with and without volunteer corn. The development and yield components of EnlistTM soybean were evaluated and physiological seed testing performed on soybean’s seeds. Clethodim controlled EnlistTM volunteer corn, whereas haloxyfop-P-methyl, pinoxaden, and cloransulam-methyl showed insufficient control levels (30-35%) at 30 days after spray. Imazethapyr exhibited 84% of control of EnlistTM volunteer corn and injured the EnlistTM soybean, affecting its seed viability. EnlistTM volunteer corn interferences negatively the soybean crop yield, yield components, and affects the plant development pattern.

Isoflavones in Soybean as a Daily Nutrient: The Mechanisms of Action and How They Alter the Pharmacokinetics of Drugs.

Soybeans [Glycine max (L.)] are a good source of isoflavones. The main isoflavone components of soybean are daidzein, genistein, and glycitein. World soybean production is very high. Because of its pharmacological activity, soy isoflavone intake over a long period of time may result in interactions with the drugs. This review summarizes soy isoflavone-drug interactions based on the pharmacokinetic parameters. Soy isoflavones have pharmacokinetic interactions with celecoxib, theophylline, paclitaxel, midazolam, imatinib, carbamazepine, valproic acid, repaglinide, omeprazole and danofloxacin. This is due to the changes in the area under the curve, maximum serum concentration, time that a drug is present at the maximum concentration in serum, clearance and half-life of the drugs when delivered together with soy isoflavones. The mechanisms of pharmacokinetic interactions occurs through the inhibition/induction of drug metabolizing cytochrome P450 (CYP450) enzymes such as CYP3A4, CYP2A1, and CYP2C9 or through the inhibition of drug transporters such as P-glycoprotein and breast cancer resistance protein. Thus, the consumption of soybean, soy isoflavones or soy products with drugs needs to be reconsidered.

Growth and yield response of local soybean in the giving of various organic fertilizer

Soybean is the basic material for any kind of industry, such as tofu and tempeh industries. Soybean cultivation will be better if use a sustainable agricultural system, such as using organic fertilizers. This research purpose was to find out the effect of organic fertilizer on the growth and yield of local soybean. The research used a complete randomized design with one factor which was a variety of organic fertilizers with 6 treatments. Various fertilizers used in this research consist of inorganic fertilizer, liquid organic, compost, cow manure, rabbit urine, chicken manure, and goat manure. Each test unit consists of 4 pots (plants) and each was repeated 4 times. The results showed that the provision of compost and goat manure increased the growth of local soybean plants, include plant height and the number of leaves at 42 DAP and leaf area at 28 DAP. The application of various organic fertilizers (liquid fertilizer, compost, cow manure, goat manure, and rabbit urine) is unable to increase the yield and yield components of local soybeans.

Slowing down of starch hydrolysis of glutinous rice and non-glutinous rice flours by black soybean extracts: Cooperation between cyanidin 3-O-glucoside and procyanidins

• Black soybean components inhibited starch hydrolysis by binding to rice flours. • The inhibition was greater in non-glutinous rice flour than glutinous rice flour. • In the components, cyanidin 3- O -glucoside and procyanidins were included. • The inhibition by cyanidin 3- O -glucoside and procyanidins were cooperative. • The cooperation took place in the digestion of amylopectin as well as amylose. Black soybean (BSB), which contains cyanidin 3- O -glucoside (C3G) and procyanidins, is an ordinary ingredient in Japan. The BSB extracts slowed down pancreatin-induced reducing sugar formation and starch hydrolysis more significantly in non-glutinous rice flour (joshin-ko) than glutinous rice flour (mochi-ko), suggesting that BSB components inhibited amylose digestion more effectively than amylopectin digestion. In the components, C3G, a procyanidin dimer (proB2), and procyanidin oligomers were included. C3G could cooperate with proB2 for the inhibition. Procyanidin oligomers inhibited the amylose hydrolysis significantly but slightly the reducing sugar formation in joshin-ko, and inhibited the both reactions slightly in mochi-ko, suggesting that the oligomers could effectively inhibit amylose digestion. C3G could also cooperate with the procyanidin oligomers to inhibit amylose digestion in joshin-ko. The above results suggest that the cooperation of C3G and procyanidins might also be included in the BSB extract-dependent inhibition of amylose digestion in joshin-ko.

QTL mapping of seed protein and oil traits in two recombinant inbred line soybean populations

ABSTRACT Seed oil and seed protein contents are commercially important components of soybean (Glycine max (L.) Merr.) that are inversely correlated. The objectives of this study were to identify novel quantitative trait loci (QTL) and validate existing QTL associated with seed oil, seed protein, and seed weight in soybean. Two mapping populations, Pop 201 and Pop 202, consisting of 180 and 170 recombinant inbred lines (RILs), respectively, were used in this study. The phenotypic data for each population were collected from four environments. The linkage maps of Pop 201 and Pop 202 consisted of 421 and 416 polymorphic single nucleotide polymorphism (SNP) markers, respectively. Multiple QTL Mapping (MQM) analyses identified a total of 13 QTL for seed oil, 7 QTL for seed protein, and 6 for seed weight (SDWT). QTL for seed oil content not co-located with protein QTL were found on chromosomes 17 and 18 in multiple environments in Pop 201 and Pop 202, respectively. These QTL can be useful in reducing the inverse correlation between seed protein and seed oil contents. Most QTL found in this study are in previously reported genomic regions, and thus provide additional evidence for the stability of those QTL across genetic and environmental backgrounds. The findings of this study provide additional insight into the genetic control of these traits and potentially enable breeders to utilize the QTL-linked SNPs in marker-assisted selection (MAS).

Overexpression of UDP-glycosyltransferase genes enhanced aluminum tolerance through disrupting cell wall polysaccharide components in soybean

Overexpression of UDP-glycosyltransferase genes enhanced aluminum tolerance through disrupting cell wall polysaccharide components in soybean

Effectiveness of the natural resistance management refuge for Bt-cotton is dominated by local abundance of soybean and maize

Genetically engineered crops expressing Bacillus thuringiensis (Bt) Cry toxins have transformed insect management in maize and cotton, reducing insecticide use and associated off-target effects. To mitigate the risk that pests evolve resistance to Bt crops, the US Environmental Protection Agency requires resistance management measures. The approved resistance management plan for Bt maize in cotton production regions requires a structured refuge of non-Bt maize equal to 20% of the maize planted; that for Bt cotton relies on the presence of an unstructured natural refuge comprising both non-Bt crop and non-crop hosts. We examined how abundance of Bt crops (cotton and maize) and an important non-Bt crop (soybean) component of the natural refuge affect resistance to Bt Cry1Ac toxin in local populations of Helicoverpa zea, an important lepidopteran pest impacted by Bt cotton and maize. We show refuge effectiveness is responsive to local abundances of maize and cotton and non-Bt soybean, and maize, in its role as a source of H. zea infesting cotton and non-Bt hosts, influences refuge effectiveness. These findings have important implications for commercial and regulatory decisions regarding deployment of Bt toxins targeting H. zea in maize, cotton, and other crops and for assumptions regarding efficacy of natural refuges.

Causation between Agronomic Traits in Homozygous Soybean Lines

Seed yield in soybean is associated with other yield components. The objective of the study was to investigate the pattern of relationship between seed yield and yield components in soybean. Agronomic characters and seed yield of 147 soybean homozygous lines (HL) and 3 check cultivars (Dega 1, Detap 1, and Anjasmoro) were evaluated in Banyuwangi (East Java, Indonesia) from April to July 2018. The field experiment was conducted in a randomized complete block design with 2 replications. After threshing, the HLs were grouped into large seed size (> 14.00 g/100 seeds) and medium seed size (10 - 14 g/100 seeds). The causation and inter-relationship between yield components and seed yield were quantified using path analysis. Based on the seed size, a total of 150 HLs was grouped into 97 lines and 53 lines of large and medium seed size, respectively. The correlation analysis showed a similar pattern between groups of large and medium seed size. The seed yield in both groups was highly determined by the characters of the number of filled pods, number of nodes, number of branches, plant height, and wet biomass. The results of path analysis revealed that plant age, particularly the length of the generative phase, was an important factor affecting seed yield in large seed soybean lines. The length of the generative phase determining the soybean yield in this study can be suggested to be used as selection criteria to obtain high yielding and large seed size variety. HIGHLIGHTS The investigation on the pattern of relationship between seed yield and yield components in soybean The path analysis to quantify the causation and inter-relationship between yield components and seed yield The soybean seed yield was highly determined by the characters of the number of filled pods, number of nodes, number of branches, plant height, and wet biomass The length of the generative phase could be used as selection criteria to obtain high yielding and large seed size soybean variety

Simultaneous and accurate screening of multiple genetically modified organism (GMO) components in food on the same test line of SERS-integrated lateral flow strip

Herein, a surface-enhanced Raman scattering (SERS)-integrated LFS platform was developed for rapid and simultaneous screening of multiple genetically modified organism (GMO) components (promoter, codon, and terminator) in soybean. Research demonstrated that, on the same test line (T line) of single LFS, three different GMP components can be well distinguished with the help of three SERS nano tags. Good linear correlations between SERS signal and concentration of each GMO component were also obtained for quantitative analysis. Of greater importance, whether these multiple analytes coexisted or not, varied in the same concentration trend or not, these multiple GMP components can be rapidly (15 min) and accurately screened with satisfied sensitivity and specificity by decoding the signals on the same T line. We envision that this decoding platform can further improve the potential of LFS and SERS for practical applications and provide a promising alternative for multiple screening of GMO identification in food.

Optimizing nitrogen supply promotes biomass, physiological characteristics and yield components of soybean (Glycine max L. Merr.).

Avoidable or inappropriate nitrogen (N) fertilizer rates harmfully affect the yield production and ecological value. Therefore, the aims of this study were to optimize the rate and timings of N fertilizer to maximize yield components and photosynthetic parameter of soybean. This field experiment consists of five fertilizer N rates: 0, 75, 150, 225 and 300 kg N ha−1 arranged in main plots and four N fertilization timings: V5 (trifoliate leaf), R2 (full flowering stage) and R4 (full poding stage), and R6 (full seeding stage) growth stages organized as subplots. Results revealed that 225 kg N ha−1 significantly enhanced grain yield components, total chlorophyll (Chl), photosynthetic rate (PN), and total dry biomass and N accumulation by 20%, 16%, 28%, 7% and 12% at R4 stage of soybean. However, stomatal conductance (gs), leaf area index (LAI), intercellular CO2 concentration (Ci) and transpiration rate (E) were increased by 12%, 88%, 10%, 18% at R6 stage under 225 kg N ha−1. Grain yield was significantly associated with photosynthetic characteristics of soybean. In conclusion, the amount of nitrogen 225 kg ha−1 at R4 and R6 stages effectively promoted the yield components and photosynthetic characteristics of soybean.

Effects of Alpha-Connexin Carboxyl-Terminal Peptide (aCT1) and Bowman-Birk Protease Inhibitor (BBI) on Canine Oral Mucosal Melanoma (OMM) Cells.

Oral mucosal melanomas (OMM) are aggressive cancers in dogs, and are good models for human OMM. Gap junctions are composed of connexin units, which may have altered expression patterns and/or subcellular localization in cancer cells. Cell-to-cell communication by gap junctions is often impaired in cancer cells, including in melanomas. Meanwhile, the upregulated expression of the gap junction protein connexin 43 (Cx43) inhibits melanoma progression. The α-connexin carboxyl-terminal (aCT1) peptide reportedly maintains Cx43 expression and cell-cell communication in human mammary cells and increases the communication activity through gap junctions in functional assays, therefore causing decreased cell proliferation. The Bowman-Birk protease inhibitor (BBI), a component of soybeans, induces Cx43 expression in several tumor cells as a trypsin–chymotrypsin inhibition function, with antineoplastic effects. This study investigated the effect of aCT1 peptide and BBI treatment, alone or in combination, on TLM1 canine melanoma cell viability. Cell viability after treatment with aCT1, the reverse sequence peptide (R-pep), and/or BBI for 5 days was analyzed by PrestoBlue assay. Immunofluorescence was used to observe Cx43 localization and expression. aCT1 (200 μM) alone did not significantly decrease cell viability in TLM1 cells, whereas BBI (400 μg/ml) alone significantly decreased the TLM1 viability. Combined treatment with both aCT1 (200 μM) and BBI (400 μg/ml) significantly decreased cell viability in TLM1 cells. Cx43 expression, as identified by immunostainings in TLM1 cells, was increased in the cell membrane after the combination treatment with BBI and aCT1. This dual treatment can be combined to achieve the anticancer activity, possibly by increasing Cx 43 expression and affecting Cx43 migration to the cell membrane. In conclusion, a treatment strategy targeting Cx43 with BBI and aCT1 may possibly lead to new effective therapies for canine OMM.