Complex Therapy Of Diseases Research Articles

Ethylmethylhydroxypyridine Succinate Is an Inhibitor but Not a Substrate of ABCB1 and SLCO1B1.

2-Ethyl-6-methyl-3-hydroxypyridine succinate (EMHPS, Mexidol) is an original antioxidant and an anti-ischemic drug with the possibility of wide applications in the complex therapy of diseases, accompanied by the development of oxidative stress and ischemia; for example, ischemic stroke, chronic cerebral ischemia, and chronic heart failure. The use of EMHPS in the complex therapy of the above diseases may cause the development of drug-drug interactions, particularly pharmacokinetic interactions at the level of transporter proteins. In the present study, we evaluated the interaction of EMHPS with ABCB1 and SLCO1B1. In Caco-2 cells, it was shown that EMHPS is not a substrate of ABCB1 and that it does not affect its expression, but at the same time, it inhibits the activity of this transporter. Its inhibitory activity was inferior to verapamil-a classic inhibitor of ABCB1. In HEK293 and HEK293-SLCO1B1 cells, it was shown that EMHPS is not a substrate of SLCO1B1 either, but that it inhibited the activity of the transporter. However, its inhibitory activity was inferior to the classic inhibitor of SLCO1B1-rifampicin. Furthermore, it was found out that EMHPS does not affect SLCO1B1 expression in HepG2 cells. The approach proposed by the FDA (2020) and the International Transporter Consortium (2010) was used to assess the clinical significance of the study results. The effect of EMHPS on SLCO1B1 and the systemic inhibition of ABCB1 by EMPHS are not clinically significant, but ABCB1 inhibition by EMHPS in the gastrointestinal tract should be tested in vivo through clinical trials.

Development of Technology for the production of finished forms of Medicinal probiotics

The study aimed to develop a technology for the production of a finished form of medicinal probiotic agents against human intestinal infections based on active strains of lactic acid and propionic acid bacteria with a wide range of biological activity and resistance to antibiotics. From the laboratory collection of lactic acid and propionic acid bacteria isolated from the intestines of healthy people, two associations of bacteria were compiled with antagonism against test cultures of Staphylococcus aureus, Salmonella gallinarum, Mycobacterium B5, Candida albicans, Pasteurella multocida, Bacillus subtilis, Escherichia coli 8739, Klebsiella pneumoniae ATCC 700603 and ATCC BAA 2524, Staphylococcus aureus 3316 and 9, Salmonella enteritidis 35382, and Pseudomonas aeruginosa 835, as well as the ability to produce hydrolytic enzymes amylase and proteinase, B vitamins, and essential amino acids. The resistance of the selected associations of lactic acid and propionic acid bacteria to the used antibiotics has been studied, which will allow for using them, if necessary, in the complex therapy of diseases. Technology for the production of probiotic medication from these associations has been developed. It was found that the most active preparation in terms of bacterial titer and antagonistic activity and the most stable one during storage for 6 months was the liquid preparation obtained by growing association No. 2 (L. plantarum 2v/A-6+L. brevis B-3/A-26+L. acidophilus 27w/60+P. shermanii 8) on nutrient medium No. 1 (De Man, Rogosa and Sharpe agar with CoCl2) using 7% sucrose and 1.5% gelatin as a protector. The liquid preparation from association No. 5 grown on medium No. 1 showed a more complete preservation of production-valuable signs during storage compared to the results of using nutrient medium No. 4, while the use of protector No. 1 was more optimal. To test the stability during the storage of dry preparation forms, an accelerated method was used by warming them up for 15 minutes at 60°C. It was found that after warming up, the best preservation of viable bacterial cells was observed in association No. 2 on nutrient media No. 1 and No. 4, in association No. 5 on medium No. 4 dried with protector No. 2 (7% sucrose and 1.5% gelatin + 7% skim milk powder), while the titer of bacteria was equal to 1.2×109, 3.5×108, and 2.0±0.2×108 colony-forming units/g, respectively. Antagonistic activity in these association variants was observed against all test cultures taken into the study with zones of suppression of their growth ranging from 10 to 24 mm.

Variety of effects of silicon dioxide colloid in the treatment of antibiotic-associated diarrhea in children

Antibiotic-associated diarrhea (AAD) is a key aspect of pediatrics concerning antibiotic therapy Purpose. The article aims at investigating the effects of colloidal silicon dioxide in the treatment of antibiotic-associated diarrhea in children. Materials and methods. Using parametric and nonparametric methods of analysis, 83 patients were examined (treatment group — 41 children who used colloidal silicon dioxide and the control group — 42 children) of both sexes (mean age (13.7 ± 2.6) years) with pathology of the lower respiratory tract (acute bronchitis or community-acquired pneumonia), who developed AAD against the background of ABT. Results. When using colloidal silicon dioxide in 75.6% of children, diarrhea decreased by the 2nd day and stopped by the 4th day (median — 0 points). Against the background of taking the drug throughout the treatment, there was a marked 3-fold decrease in all AAD values. The assessment of the efficacy and safety of the drug according to the data of medical researchers was 3.4 points. Analysis of subjective data on the tolerability of the drug showed «excellent» and «good». Against the background of the full course of treatment with colloidal silicon dioxide, a 2 times more pronounced decrease in the characteristics of AAD was noted. Conclusion. The presented data are intended to focus the attention of pediatricians on the rational use of ABT, timely diagnosis of an unwanted drug reaction in the form of AAD and its correction. The studied drug showed high efficacy and safety, which makes it possible to recommend it as a symptomatic treatment of diarrhea that developed against the background of ABT treatment in the complex therapy of diseases of the lower respiratory tract in children.

Acoustic assessment of pulmonary ventilation in children with bronchial asthma on the background of a single exposure to microwave radiation

Summary. Diseases of the respiratory organs of children, occurring with obstructive syndrome, represent one of the most serious problems in terms of transition to chronic forms. The chronic process is accompanied by a decrease in pulmonary ventilation, which leads to the search for effective methods of complex therapy of diseases.
 The purpose of this study was to evaluate the dynamics of acoustic indicators of pulmonary ventilation in children with bronchial asthma, against the background of non-invasive non-thermal microwave radiation.
 Materials and methods. 122 children with bronchial asthma aged from 3 to 17 years were examined. 113 patients underwent a single session of non-invasive microwave therapy with the Aster device. Comparison of acoustic characteristics of pulmonary ventilation was carried out taking into account the severity of the disease and the age of children with bronchial asthma. Acoustic assessment of ventilation function was performed using the computer bronchophonography complex Pattern-01 before the Aster procedure, after 5 and 15 minutes. In 20 (16.4%) patients with bronchial asthma, the dynamics of cough was evaluated against the background of Aster therapy for 7 days.
 Results. Improvement of bronchial patency was registered in all age groups with mild bronchial asthma. In the group of moderate asthma, exposure to electromagnetic radiation led to a significant reduction in ventilation disorders in children aged 711 years. As patients grow older, as well as with severe bronchial asthma, the smallest dynamics of the parameters of pulmonary ventilation was observed against the background of Aster therapy. The use of electromagnetic radiation in addition to traditional basic therapy for 7 days leads to a faster regression of cough in patients with bronchial asthma.
 Conclusion. The combination of traditional basic therapy with electromagnetic non-thermal radiation in patients with bronchial asthma reduces obstructive pulmonary ventilation disorders and promotes faster cough regression.

Фізична культура при серцево-судинних захворюваннях

The article is devoted to the study of the problems of diseases of the cardiovascular system and methods for their treatment and prevention using physical culture, as well as the problems of the influence of physical therapy on the development of adaptive reactions of the cardiovascular system in patients with coronary heart disease. The purpose of the article is to identify the complex of physical exercises and their influence on diseases of the cardiovascular system. Recently, in the medical field, the rehabilitation direction, the core of which is the step-by-step, restorative complex treatment of diseases and injuries, has been increasingly approved. In this process, an important role is played by therapeutic physical culture as a method of active, functional and pathogenetic therapy. It aims at improving the quality of treatment, preventing possible complications in various diseases and injuries, accelerating the recovery of organs and systems, training and quenching the body, restoring performance, reducing the likelihood of disability. Reducing the time of recovery, reducing material costs for inpatient and sanatorium-spa treatment are possible only with the correct selection of means and forms of physical therapy, which is the most important and effective part of the whole system of medical rehabilitation. Modern therapeutic physical culture is based on fundamental concepts and medical and biological substantiation of mechanisms of influence of physical exercises on systems of maintenance of homeostasis of an organism, their adaptation to new conditions of existence, or morpho-functional changes of an organism after traumas and transferred illnesses. The purpose of the article is to identify the complex of physical exercises and their influence on diseases of the cardiovascular system. The use of physical training and massage for healing purposes has a long history. For many centuries, complex therapy of diseases has been carried out with the help of physical exercises, massage, hardening, baths, hydroprocessing, metered walking, and the like. Despite the fact that medicine did not yet have accurate data on the adverse effects of hypodynamia, doctors at that time recommended the activation of the motor regime for certain diseases and abnormalities in health.

Possibility of complex rehabilitation of chronic pancreatitis at the stage of primary medical care (literature review)

Prevention of exacerbation and extension of remission of disease are crucial for the further rehabilitation of patients with chronic pancreatitis. Change of lifestyle is the first and the most important step in the prevention of repeated exacerbations of disease: stopping alcohol consumption and smoking, diet. In our time of the increasing number of diseases associated with the side effects of drugs and severe allergic reactions caused by them, physiotherapeutic methods of treatment can play key role in the complex therapy of diseases of internal organs: climate therapy, physiotherapy (yoga is effective in reducing the pain perception and increasing the body mass), hydrotherapy and treatment by heat (mineral water intake), peloidotherapy, methods of outfit physiotherapy. Phyto- and apitherapeutic agents, hirudotherapy, which are coming back to clinical practice, as well as reflexotherapeutic techniques, can be used for the treatment and rehabilitation of patients with chronic pancreatitis at the present stage of evolution. It should be taken into account that patients often have psychoemotional disorders, which can be considered upon choosing the differentiated rehabilitation programs, including psychotropic drugs and psychotherapy techniques in the traditional therapy of pancreatitis.