Copper(II) complexes of [Cu(II)(1-amidino-O-2-methoxyethylurea)2]Cl2 (1) and [Cu(II)(1-amidino-O-2-ethoxyethylurea)2]Cl2 (2) have been synthesized and characterized by elemental analysis, magnetic moment, conductance, thermal analysis, IR, UV, powdered XRD and EPR spectral studies. The trend in g values g|| > g⊥ > 2.0023 in the EPR spectra of the complexes suggest that the unpaired electron on copper(II) ion has \({d_{x}}^2 - {{}_{y}}^2\) character and the complexes have square planar structure which is also supported by electronic absorption spectral studies. The bonding parameters of these complexes in DMF have been calculated based on the EPR and electronic spectral data. The complexes (1) and (2) exhibit K|| > K⊥ which indicates the presence of significant out-of-plane π bonding. Powdered XRD spectral patterns show that complexes crystallize in a monoclinic system. The binding of the complexes with calf thymus DNA was investigated by absorption and emission spectroscopy, viscosity measurements, DNA melting and cyclic voltammetric studies. DNA interaction studies show that the complexes bind to calf thymus DNA in a non-intercalative mode. The two complexes exhibit growth inhibitory activity against pathogenic bacteria, viz., Escherichia coli, Klebsiella pneumoniae sub sp. Pneumoniae, Proteusmirabilis.