Complete Life Cycle Research Articles

Morphological Characterization of Cannabis sativa L. Throughout Its Complete Life Cycle.

This study extensively characterizes the morphological characteristics, including the leaf morphology, plant structure, flower development, and trichome features throughout the entire life cycle of Cannabis sativa L. cv. White Widow. The developmental responses to photoperiodic variations were investigated from germination to mature plant senescence. The leaf morphology showed a progression of complexity, beginning with serrations in the 1st true leaves, until the emergence of nine leaflets in the 6th true leaves, followed by a distinct shift to eight, then seven leaflets with the 14th and 15th true leaves, respectively. Thereafter, the leaf complexity decreased, culminating in the emergence of a single leaflet from the 25th node. The leaf area peaked with the 12th leaves, which coincided with a change from opposite to alternate phyllotaxy. The stipule development at nodes 5 and 6 signified the vegetative phase, followed by bract and solitary flower development emerging in nodes 7-12, signifying the reproductive phase. The subsequent induction of short-day photoperiod triggered the formation of apical inflorescence. Mature flowers displayed abundant glandular trichomes on perigonal bracts, with stigma color changing from whitish-yellow to reddish-brown. A pronounced increase in trichome density was evident, particularly on the abaxial bract surface, following the onset of flowering. The trichomes exhibited simultaneous growth in stalk length and glandular head diameter and pronounced shifts in color. Hermaphroditism occurred well after the general harvest date. This comprehensive study documents the intricate photoperiod-driven morphological changes throughout the complete lifecycle of Cannabis sativa L. cv. White Widow. The developmental responses characterized provide valuable insights for industrial and research applications.

An application-oriented digital twin framework and the multi-model fusion mechanism

ABSTRACT The digital twin (DT) technology facilitates the complete lifecycle management of equipment by integrating physical and virtual spaces through data mapping. Many narrative DT frameworks and modeling methods have been proposed. However, the heterogeneous processes and methods of applying these DT frameworks in different objects and different scenarios of manufacturing restricts the function and promotion of DT. Given that there are some existing discussions on narrative DT frameworks, this paper proposes an application-oriented DT framework that integrates information models, principle models, and field models. Then, the unified DT application process is discussed. The mechanism of how to fuse the multi models for typical applications in evaluation, prediction, and optimization are elaborated in detail respectively. Finally, the proposed framework and application process are validated through two DT models: vehicle wheel polygonal diagnosis digital twin and bogie frame manufacturing optimization digital twin. The correctness and feasibility of the proposed approach is demonstrated through these case studies.

Rebound effects undermine carbon footprint reduction potential of autonomous electric vehicles

Autonomous vehicles offer greater passenger convenience and improved fuel efficiency. However, they are likely to increase road transport activity and life cycle greenhouse emissions, due to several rebound effects. In this study, we investigate tradeoffs between improved fuel economy and rebound effects from a life-cycle perspective. Our results show that autonomy introduces an average 21.2% decrease in operation phase emissions due to improved fuel economy while manufacturing phase emissions can surge up to 40%. Recycling efforts can offset this increase, cutting emissions by 6.65 tons of Carbon dioxide equivalent per vehicle. However, when examining the entire life cycle, autonomous electric vehicles might emit 8% more greenhouse gas emissions on average compared to nonautonomous electric vehicles. To address this, we suggest; (1) cleaner and more efficient manufacturing technologies, (2) ongoing fuel efficiency improvements in autonomous driving; (3) renewable energy adoption for charging, and (4) circular economy initiatives targeting the complete life cycle.

The biphasic life cycle of the non-spinose planktonic foraminifera is characterised by an aberrant coiling signature

The complete lifecycle of the planktonic foraminifera has continued to remain obscure. Benthic foraminifera exhibit an obligate alternation of generations between haploid gamonts and diploid agamonts, yet despite years of observation, the fusion of gametes to form a zygote, gamont or agamont has never been observed in the planktonic groups. Recent evidence from Neogloboquadrina pachyderma culture confirms that they do have a biphasic reproductive cycle and the agamonts may exhibit dimorphism in coiling direction. In this study we examine the morphology of normal and aberrant coiling tests of both N. pachyderma and N. incompta from three Arctic and North Atlantic water masses, to explore whether aberrant coiling is an indicator of a biphasic life cycle in the non-spinose planktonic foraminifera group as a whole. Using a Nano-Computed Tomography scanning approach, we morphologically compared the proloculus and growth trajectories of aberrant and normal coiling tests. Aberrant coiling tests were found to have a slightly larger proloculus and test size, consistent with a left coiling N. pachyderma agamont in the literature. This degree of dimorphism is insufficient to discriminate agamonts in the wild population and coiling direction remains their only distinguishable feature without further morphological investigation. The constant low-level aberrant coiling signature in a range of non-spinose planktonic foraminiferal genera implies that a biphasic life cycle is the principal mode of reproduction in the non-spinose planktonic group.

Seasonal Dynamics and Physiological Age of Ixodid Ticks Collected from Dogs.

In order to reproduce and complete life cycles, ticks have to feed on different hosts, thus participating as vectors and reservoirs in the maintenance and circulation of different pathogens. Since dogs can serve as suitable hosts for numerous tick species, the aims of this study were to determine tick species and their seasonal occurrence on pet dogs and to compare the accuracy of three indices frequently used to calculate engorged female physiological age. Ticks were collected from dogs brought to veterinary clinics. Three indices were analyzed: scutal index, alloscutal/scutal index ratio, and physiological age index. Four tick species were identified: Ixodes ricinus, Dermacentor marginatus, D. reticulatus, and Rhipicephalus sanguineus group, and the last was the most abundant. The highest number of collected ticks was in April, but two species were continuously active throughout the year. The statistical analyses distinguished the physiological age index as more precise because of lower variability. Dog owners usually ignore regular dog anti-tick treatments throughout the year, as they are not aware that ticks could be active during the winter months. Tick surveillance is unquestionably important in order to monitor and prevent the distribution of these vectors and also the diseases they transmit.

The Role of In‐Cloud Wet Removal in Simulating Aerosol Vertical Profiles and Cloud Radiative Forcing

AbstractAmong the physical processes controlling aerosol vertical profiles, in‐cloud wet removal is of utmost importance while its representation in global climate models (GCMs) is crude. In this study, we implement into the Community Atmosphere Model version 6 (CAM6) a physically‐based aerosol wet removal parameterization scheme that explicitly treats aerosol activation, removal and resuspension. Evaluation against in‐situ observations shows that the default scheme substantially overestimates the upper tropospheric black carbon (BC) and sea salt mass. Our physically‐based scheme reduces BC and sea salt mass by a factor of 10 and 1,000, respectively, in better agreement with observations. Also, the new scheme slightly increases number of aerosol particles between 12 nm and 4.8 μm in diameter, thereby mitigating the aerosol number underestimation in the default scheme. Our new scheme reduces the overestimation of coarse‐mode aerosol (0.5–4.8 μm) number. Overall, the aerosol property changes (mass decrease and number increase) reduce the cloud condensation nuclei (CCN) concentration at low supersaturation (i.e., 0.02% and 0.1%), and increase CCN at high supersaturations (i.e., 0.5% and 1%). Consequently, the global annual mean cloud liquid water path increases by 1.89 g m−2 and the ice water path increases by 0.51 g m−2. The global annual mean shortwave, longwave, and net cloud radiative forcing change by −1.06, 0.57, and −0.48 W m−2, respectively. Further improvement is needed to reflect the real physics that the removal efficiencies for aerosol mass and number are disproportionate and to advect cloud‐borne (activated) aerosols for a complete aerosol lifecycle.

A Dynamic Bayesian Network Probability Framework for Typhoon Risk Analysis in the Land-sea Regions

The life cycle of a typhoon is mainly divided into three stages: far away from the coast, offshore, and on land. The disaster bodies and typhoons affected are different at every stage. The traditional assessment methods could not consider the risk from the complete typhoon life cycle to cover the effects of both land and sea. This paper proposes a Bayesian network risk assessment model to evaluate the navigational ship risk and damage risk of coastal structures during the different typhoon life cycles. The disaster memory database of the typhoon is also constructed in this paper, which deconstructs the typhoon risk factors and forms a unified description semantics.

TOWARDS BIM-GIS INTEGRATION FOR ROAD INTELLIGENT MANAGEMENT SYSTEM

Roads and highways are often managed by using Geographic Information Systems (GIS). However, these systems lack the level of detail that Building Information Modelling (BIM) can bring to an infrastructure management system. BMGIS integration allows the management of information from both infrastructure and environmental points of view. This provides an overview of the infrastructure, facilitating decision-making process throughout its complete life cycle. This article shows a semi-automated process to generate the hybrid BIM-GIS model of Madrid Calle30. The model together with an external database was uploaded to an intelligent management platform that allows visualising the available documentation, assisting management, and bringing the ring-road closer to a digital twin of the infrastructure.

ESCAPE simplified approach designed to evaluate materials sustainability: The case of new adsorbent materials for activated carbon substitution

This paper discusses a novel simplified approach for assessing the sustainability of new material, specifically focusing on the synthesis technology used. The material can be obtained through emerging technologies, even if they are in the early laboratory development stage. The evaluation is conducted by comparing it to a reference material, which could be a natural resource, for instance. This approach can be applied in situations where complete life cycle assessment (LCA) data is unavailable or a comprehensive LCA evaluation is not required. ESCAPE (standing for Evaluation of Sustainability of material substitution using CArbon footPrint by a simplifiEd approach) involves the calculation of two parameters, the embodied energy, and the CO2 footprint, for all steps of the material synthesis procedure. The manuscript discusses in detail for the first time the approach's applicability and potentialities with some examples, devoted to new proposed adsorbents, derived from wastes. For this aim, 50 literature papers are considered and analysed. Surprisingly, the results show that only 28% of the 50 selected materials are in accord with the introduced sustainability index, showing that most of the new proposed adsorbents appear to be not environmentally sustainable.This study's findings highlight the ESCAPE approach's potential in facilitating materials eco-design by identifying the most resource-intensive steps in synthesis procedures. Notably, this approach provides the flexibility to incorporate the energy mixes of different Countries where the new processes may be deployed, enabling sustainability evaluations based on specific locations. This capability opens possibilities for a more comprehensive and context-specific assessment of sustainability in materials development. In essence, ESCAPE serves as a preliminary screening method before conducting an LCA, enabling rapid evaluations. It serves as a valuable tool for researchers and industries aiming to develop sustainable materials, thereby expediting the transition toward a circular economy.

English

Crop mapping and its health monitoring are of key importance for a country like Pakistan where a considerable share of the GDP originates from agricultural production. Currently, extensive capital and human resource are utilized to record the crop types and monitor crop health due to variations in crop pattern and intercropping practices being observed in many parts of the country. Satellite Remote Sensing, however, can be utilized very efficiently to identify crop types and estimate acreage by using unique phenological cycles derived from NDVI thresholds. In this research, we proposed a semi-automated, efficient and cost-effective state-of-the-art technique for crop classification using satellite remote sensing datasets and GIS techniques to replace outdated conventional survey-based crop estimations procedures. This research was conducted in Sahiwal district, Pakistan to categorise several crop types using two major inputs: satellite (Sentinel-2, 10m) imagery and local crop calendar. The research adopted a semi-automatic approach to find the main crops of Rabi (winter) and Kharif (summer) using orthorectified and atmospherically corrected Level-1C images to extract phenological information based on Normalized Difference Vegetation Index (NDVI). The complete phenological life cycle of crops including sowing, growing, and harvesting stages were deeply analyzed with the help of crop calendar and NDVI profiles. Then this information passed through iterative selforganizing (ISO Data Model) of unsupervised classification technique in the form of temporal stacked images. The NDVI profiles of each crop were synchronized with the crop calendar of the study area to develop the crop categorization procedure. The results were validated with the statistics of the agriculture department Lahore and field samples gave about 85% and 88% accuracy for Rabi and Kharif cropping seasons respectively. It is concluded from the research that the tested methodology is capable of providing a fast, cost-efficient, and reliable solution for crop mapping instead of conventional survey-based crop area assessment techniques (conventional Girdawri) which requires a vast number of resources and time, and it can be replicated in different areas with minor changes

Studies on Biology, Morphometrics and Substrate Preference of the Burrowing Cockroach <i>Pycnoscelus surinamensis</i> (L.)

Biology of the burrowing cockroach Pycnoscelus surinamensis (L.) revealed that there are four instars and took 134.8±4.94 days to complete lifecycle. Morphometric studies showed increase in antennal body length, head width, pronotum length and width from first to fourth instar with peak at adult and other developmental stages well fit into Brooks-Dyar rule. All body parameters showed hypoallometry except pronotum length which showed hyperallometry. In substrate preference studies, the survival was lowest for the treatment without substrate and against the treatments with substrates like leaf litter, cocopeat and vermiculite. The mean number of offspring produced and survived was found to be lower in the treatments without substrate and with leaf litter against the treatments with cocopeat and vermiculite.

Sustainable management in the slow fashion industry: carbon footprint of an Italian brand

PurposeEnvironmental impacts associated with the fashion industry concern society and require commitment to sustainable development goals from leading companies. The role of the luxury sector in setting trends and negotiating power within the supply chain can lead this industry towards sustainability. This study constructs a comprehensive operational flux inventory attributed to an Italian luxury garment brand, aiming to investigate and propose feasible strategies to reduce potential impacts coupled with their products.MethodsUnder the operational control criteria, a whole year of activities was tracked using mainly primary data from its management system. According to ISO 14064–1:2019, potential greenhouse gas emissions were classified, organized, and processed into six categories. The analysis, at the company level, covered the product’s complete life cycle, i.e., from cradle to the grave. The ecoinvent database considered preferentially local geography, and the cut-off system approach, therefore assigning emissions to the primary user.Results and discussionResults showed that the only unit in central Italy where the headquarter is located (excluding retail stores), producing 485,193 women’s clothing in a year, emitted 9804 t CO2 eq. Most of these impacts (69% or 6752 t CO2 eq) can be associated with indirect emissions related to raw products and materials, and about 93% of this amount results from the high-quality products used by the company. Transportation represents 14% of the total emissions, while the use phase accounts for about 13%. As a final step, six different mitigation scenarios were proposed and analyzed by focusing on non-core production activities, i.e., upstream, and downstream operations, and consumers’ habits. Once combined, these strategies can potentially reduce by about 25% the study case company overall emissions.ConclusionsAs a conclusion, exploring possible alternatives through environmental assessment tools can support strategies for achieving impact reduction. While aggressive changes can be done in non-core activities with excellent results, changes perceived by the customers can also be well desired to mark innovation and advances in the business mindset.

Cellular Factors Involved in the Hepatitis D Virus Life Cycle.

Hepatitis D virus (HDV) is a defective RNA virus with a negative-strand RNA genome encompassing less than 1700 nucleotides. The HDV genome encodes only for one protein, the hepatitis delta antigen (HDAg), which exists in two forms acting as nucleoproteins. HDV depends on the envelope proteins of the hepatitis B virus as a helper virus for packaging its ribonucleoprotein complex (RNP). HDV is considered the causative agent for the most severe form of viral hepatitis leading to liver fibrosis/cirrhosis and hepatocellular carcinoma. Many steps of the life cycle of HDV are still enigmatic. This review gives an overview of the complete life cycle of HDV and identifies gaps in knowledge. The focus is on the description of cellular factors being involved in the life cycle of HDV and the deregulation of cellular pathways by HDV with respect to their relevance for viral replication, morphogenesis and HDV-associated pathogenesis. Moreover, recent progress in antiviral strategies targeting cellular structures is summarized in this article.

Genomes and transcriptomes help unravel the complex life cycle of the blastoclad fungus, Coelomomyces lativittatus, an obligate parasite of mosquitoes and microcrustaceans

ABSTRACT Species of the phylum Blastocladiomycota, early-diverging zoosporic (flagellated) lineages of fungi, are vastly understudied. This phylum includes the genus Coelomomyces, which consists of more than 80 fungal species that are obligate parasites of arthropods. Known Coelomomyces species lack a complete asexual life cycle, instead surviving through an obligate heterecious alternation of generations life cycle. Despite their global distribution and interesting life cycle, little is known about the genomics of any Coelomomyces species. To address this, we generated three draft-level genomes and annotations for C. lativittatus representing its haploid meiospore, orange gamete, and amber gamete life stages. These draft genome assemblies ranged in size from 5002 to 5799 contigs, with a total length of 19.8–22.8 Mb and a mean of 7416 protein-coding genes. We then demonstrated the utility of these genomes by combining the draft annotations as a reference for analysis of C. lativittatus transcriptomes. We analyzed transcriptomes from across host-associated life stages, including infected larvae and excised mature sporangia from the mosquito Anopheles quadrimaculatus. We identified differentially expressed genes and enriched GO terms both across and within life stages and used these to make hypotheses about C. lativittatus biology. Generally, we found the C. lativittatus transcriptome to be a complex and dynamic expression landscape; GO terms related to metabolism and transport processes were enriched during infection and terms related to dispersal were enriched during sporulation. We further identified five high mobility group (HMG)-box genes in C. lativittatus, three belonging to clades with mating type (MAT) loci from other fungi, as well as four ortholog expansions in C. lativittatus compared with other fungi. The C. lativittatus genomes and transcriptomes reported here are a valuable resource and may be leveraged toward furthering understanding of the biology of these and other early-diverging fungal lineages.

Insight into the biochemical and cell biological function of an intrinsically unstructured heat shock protein, Hsp12 of Ustilago maydis.

Small heat shock proteins (sHsps) play diverse roles in the stress response and maintenance of cellular functions. The Ustilago maydis genome codes for few sHsps. Among these, Hsp12 has previously been demonstrated to be involved in the pathogenesis of the fungus by our group. In the present study we further investigated the biological function of the protein in the pathogenic development of U. maydis. Analysis of the primary amino acid sequence of Hsp12 in combination with spectroscopic methods to analyse secondary protein structures revealed an intrinsically disordered nature of the protein. We also carried out detailed analysis on the protein aggregation prevention activity associated with Hsp12. Our data suggest Hsp12 has trehalose-dependent protein aggregation prevention activity. Through assaying the interaction of Hsp12 with lipid membranes in vitro we also showed the ability of U. maydis Hsp12 to induce stability in lipid vesicles. U. maydis hsp12 deletion mutants exhibited defects in the endocytosis process and delayed completion of the pathogenic life cycle. Therefore, U. maydis Hsp12 contributes to the pathogenic development of the fungus through its ability to relieve proteotoxic stress during infection as well as its membrane-stabilizing function.

Cell Culture Systems for Studying Hepatitis B and Hepatitis D Virus Infections.

The hepatitis B virus (HBV) and hepatitis D virus (HDV) infections cause liver disease, including hepatitis, cirrhosis, and hepatocellular carcinoma (HCC). HBV infection remains a major global health problem. In 2019, 296 million people were living with chronic hepatitis B and about 5% of them were co-infected with HDV. In vitro cell culture systems are instrumental in the development of therapeutic targets. Cell culture systems contribute to identifying molecular mechanisms for HBV and HDV propagation, finding drug targets for antiviral therapies, and testing antiviral agents. Current HBV therapeutics, such as nucleoside analogs, effectively suppress viral replication but are not curative. Additionally, no effective treatment for HDV infection is currently available. Therefore, there is an urgent need to develop therapies to treat both viral infections. A robust in vitro cell culture system supporting HBV and HDV infections (HBV/HDV) is a critical prerequisite to studying HBV/HDV pathogenesis, the complete life cycle of HBV/HDV infections, and consequently identifying new therapeutics. However, the lack of an efficient cell culture system hampers the development of novel antiviral strategies for HBV/HDV infections. In vitro cell culture models have evolved with significant improvements over several decades. Recently, the development of the HepG2-NTCP sec+ cell line, expressing the sodium taurocholate co-transporting polypeptide receptor (NTCP) and self-assembling co-cultured primary human hepatocytes (SACC-PHHs) has opened new perspectives for a better understanding of HBV and HDV lifecycles and the development of specific antiviral drug targets against HBV/HDV infections. We address various cell culture systems along with different cell lines and how these cell culture systems can be used to provide better tools for HBV and HDV studies.

SvTPM: SGX-Based Virtual Trusted Platform Modules for Cloud Computing

Virtual Trusted Platform Modules (vTPMs) are widely used in commercial cloud platforms (e.g., VMware Cloud, Google Cloud, and Microsoft Azure) to provide virtual root-of-trust and security services for virtual machines. Unfortunately, current state-of-the-art vTPM implementations for cloud computing cannot provide strong protection for vTPMs at run-time and suffer from poor performance under binding vTPMs to a physical TPM. In this paper, we propose SvTPM, an SGX-based virtual trusted platform module, which provides complete life cycle protection of vTPMs in the cloud and does not rely on the physical TPM. SvTPM provides strong isolation protection so malicious cloud tenants or even cloud administrators cannot access vTPM's private keys or any other sensitive data. In this paper, we implement a prototype of SvTPM, which identifies and solves a couple of critical security challenges for vTPM protection with SGX, such as NVRAM rollback attacks, NVRAM binding attacks, and vTPM rollback attacks. SvTPM also shows how to establish trust between vTPM and SGX Platform. Our performance evaluation shows that the NVRAM launch time of SvTPM is <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$1700\times$</tex-math></inline-formula> faster than vTPM built upon hardware TPM. In TPM standard command evaluation, we find that SvTPM incurs negligible performance overhead while providing strong isolation and protection. To our knowledge, SvTPM is the first practical work to solve the critical security challenges of securing vTPM using SGX.

Numerical study of the evolution of bubbles during nucleation and droplets during condensation on a surface of variable wettability using the pseudopotential MRT-LBM method

In this study, the behavior of bubbles and droplets during boiling and condensation processes on a wettable horizontal plate with variable density ( ) is investigated using pseudopotential lattice Boltzmann model for multiphase flow. The analysis focuses on the complete life cycle of bubbles and droplets, from their appearance to their disappearance under the effect of gravity. The main objectives of this study are to analyze the propagation of bubbles or droplets on the horizontal surface (l*) and to study their evolution as a function of time, characterized by h*, for different values of the wetting surface density. To ensure the reliability of our model in simulating these phenomena, the hydrodynamic effect and Laplace’s law were. This combination of approaches demonstrated a good compromise between our results and the references, confirming the model’s reliability. Furthermore, we present results that showcase the density behaviors for different wetting surfaces, allowing us to analyze droplet behavior on the horizontal plate. It was observed that for large values of significant droplet spreading occurred, and this process took time to subside. Conversely, for small values of spreading was minimal, enabling rapid droplet detachment. Interestingly, in the case of boiling, the observed behaviors were reversed compared to condensation. Moreover, the heat flux analysis for the single-density case provided insights into the temperature behavior around bubbles during the nucleation process and droplets during the droplet condensation process, while maintaining a fixed density value on average. These findings contribute to a better understanding of the thermal dynamics associated with boiling and condensation phenomena.

Peptidyl-prolyl cis/trans isomerase Pin1 interacts with hepatitis B virus core particle, but not with HBc protein, to promote HBV replication.

Here, we demonstrate that the peptidyl-prolyl cis/trans isomerase Pin1 interacts noncovalently with the hepatitis B virus (HBV) core particle through phosphorylated serine/threonine-proline (pS/TP) motifs in the carboxyl-terminal domain (CTD) but not with particle-defective, dimer-positive mutants of HBc. This suggests that neither dimers nor monomers of HBc are Pin1-binding partners. The 162TP, 164SP, and 172SP motifs within the HBc CTD are important for the Pin1/core particle interaction. Although Pin1 dissociated from core particle upon heat treatment, it was detected as an opened-up core particle, demonstrating that Pin1 binds both to the outside and the inside of the core particle. Although the amino-terminal domain S/TP motifs of HBc are not involved in the interaction, 49SP contributes to core particle stability, and 128TP might be involved in core particle assembly, as shown by the decreased core particle level of S49A mutant through repeated freeze and thaw and low-level assembly of the T128A mutant, respectively. Overexpression of Pin1 increased core particle stability through their interactions, HBV DNA synthesis, and virion secretion without concomitant increases in HBV RNA levels, indicating that Pin1 may be involved in core particle assembly and maturation, thereby promoting the later stages of the HBV life cycle. By contrast, parvulin inhibitors and PIN1 knockdown reduced HBV replication. Since more Pin1 proteins bound to immature core particles than to mature core particles, the interaction appears to depend on the stage of virus replication. Taken together, the data suggest that physical association between Pin1 and phosphorylated core particles may induce structural alterations through isomerization by Pin1, induce dephosphorylation by unidentified host phosphatases, and promote completion of virus life cycle.

Complete life cycle of Parorchis trophoni sp. nov. (Digenea: Philophthalmidae) from the Southwestern Atlantic coast, Argentina, revealed by morphological and molecular data

Complete life cycle of Parorchis trophoni sp. nov. (Digenea: Philophthalmidae) from the Southwestern Atlantic coast, Argentina, revealed by morphological and molecular data