Publisher Summary This chapter discusses that study on genetic recombination in phage T4 shows the participating DNA molecules are broken and rejoined during the process. It appears, likely, that breakage and rejoining may account for all recombination, as the extent of breakage, as measured by physical methods, is in rather good agreement with the extent of exchange, as measured by genetic methods. In genetic crosses, there are on the average 20 exchanges per genome and these are apparently clustered to some extent. Isotope transfer experiments indicate that there is a bimodal distribution of parental DNA; progeny particles, which receive any parental label, receive on the average 5–10%, or less than 1% of a chromosomal equivalent. The chapter explores that the joining of parental fragments occurs in several steps, which are mediated by specific enzymes. In intermediate structures, complementary sections of different parental origin are held together by hydrogen bonds involved in base pairing. Subsequently, the parental fragments become covalently linked. Physically hybrid regions in base-paired structures may be genetically heterozygous. They become homozygous only after the hybrid DNA is replicated or repaired. Average recombination frequencies per unit length DNA are higher in T4 crosses than in most other systems. One map unit corresponds to 100–200 nucleotide pairs, both according to physical and genetic measurements. However, the average recombination frequency per unit length DNA is not equal over the entire T4 genome.
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