Comparison Of Hematological Parameters Research Articles

Comparison of hematologic parameters as predictors of severity in COVID-19: A single-center cross-sectional study

COVID-19 disease was a global pandemic that marred humanity globally from 2019 end till early 2023. The diseases caused by the novel SARS cov2 virus had a wide array of presentations, from asymptomatic to mild illness to severe acute respiratory illness, with patients presenting with ‘happy hypoxia and sudden deterioration of clinical condition to multiorgan involvement and proven to be fatal in many cases. There was an effort to identify a point-of-care test to screen patients so as to predict the disease course; therefore, this study was designed to compare hematologic parameters to predict the severity of the disease. This study showed that TLC,dNLR, and NLR can be effectively used in diagnosing severe COVID-19.

The effect of indicators of CALLY index on survival in glioblastoma.

Glioblastoma is the most common primary brain tumor in adults. Recently, research has been published on the potential prognostic indicators associated with different types of cancer. Due to the limited availability of data investigating the relationship between the CALLY index and glioblastoma patients, we aimed to conduct this study. Between January 2017 and December 2023, we conducted a study on patients diagnosed with glioblastoma. We collected demographic data and routine laboratory tests at the time of admission. To calculate the CALLY index, we used the formula (albumin value × lymphocyte count) / CRP value × 104. Parameters were compared for in-hospital mortality across different groups. The study analyzed 202 patients who met the inclusion criteria. Of these, 165 (81.7%) were classified as "survivors" and 37 (18.3%) as "deceased." A comparison of hematologic parameters between the two groups showed a significantly lower CALLY index in the "deceased" group (3.05 (4.92)) compared to the "survivor" group (10.13 (13.69)) (p < 0.001). The study compared the parameters between groups with regard to in-hospital mortality. Based on the results of the study, we conclude that the CALLY index can be considered an easily applicable indicator for the mortality of glioblastoma patients.

Relationship Between Abdominal Aortic Aneurysm and Inflammatory Markers

Objective: In this study, we aimed to investigate the relationship between abdominal aortic aneurysm and systemic immune inflammation index (SII), neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR) and other inflammatory markers, especially those derived from complete blood count. &#x0D; Methods: Retrospectively, 72 consecutive patients admitted to our clinic for Abdominal Endovascular Aneurysm Repair (EVAR) for abdominal aortic aneurysm (AAA) between January 2019 and January 2022 were included in the study. Routine blood samples were taken before EVAR operation. NLR, PLR, SII of the patients were calculated and, RDW, Mean Platelet Volume (MPV), C-reactive protein values and other laboratory tests were recorded. They were compared with an age-matched control group.&#x0D; Results: The age of the patients included in the study was 67.7 ± 10.6 years and the majority were male. The frequency of hypertension and coronary artery disease was also higher. In the comparison of hematologic parameters with the control group, MPV was 10.4(9.6-11.2) versus 9.5(8.6-10.1), p

Association of methionine synthase reductase (MTRR A66G) polymorphism with susceptibility to acute lymphoblastic leukemia

Background and Objectives. The enzyme methionine synthase reductase is involved in cellular methylation reactions, DNA synthesis, and epigenetic processes. It is encoded by the MTRR gene, which garnered a lot of attention in current medical genetics research. This study was conducted to study the association between MTRR (A66G) polymorphism and the risk of developing acute lymphoblastic leukemia among Sudanese patients. Materials and Methods. This is a case-control study in which 150 patients with acute lymphoblastic leukemia (ALL) and 150 healthy participants as a control group were enrolled. DNA was extracted and analyzed for the MTRR (A66G) polymorphism using the real-time polymerase chain reaction. Results. Based on flow cytometry results, B-ALL was more common (79%) than T-ALL (21%). The comparison of hematological parameters in acute lymphoblastic leukemia subtypes showed a statistically significant high mean total white blood count (P=0.000) and mean blast percentage (P=0.050) in patients with T-ALL. The molecular analysis showed that the incidence of the MTRR homozygous genotypes AA and GG were higher in the patients (44% and 9.3%, respectively) compared to the control group (40% and 6.7%, respectively). In comparison, the heterozygous genotype AG was lower in the patients (46.7%) than in the control group (53.3%). However, the association between the polymorphism and acute lymphoblastic leukemia risk was not statistically significant (OR: 1.179, 95% CI 0.7459-1.865, P=0.445). Conclusions. This study concluded that MTRR A66G polymorphism was not associated with the risk of acute lymphoblastic leukemia among the Sudanese population.

Molecular analysis and clinical significance of hemoglobin Quong Sze in Huizhou city, Southern China.

Hemoglobin Quong Sze (Hb QS) is one of the most common non-deletional α-thalassemia (α-thal), which is prevalent in the Southern Chinese population. However, there are still few comprehensive researches on the molecular characterization of Hb QS. So it is important to find out appropriate diagnosis and characterization of Hb QS carrier for genetic counseling. A hematological screening including hematological indices and hemoglobin analysis was performed in 113,400 individuals from Huizhou city, Southern China. Then, suspected thalassemia carriers were detected by a suspension-array system and DNA sequencing for α- and β-thal. In our study, we identified 521 subjects who were Hb QS carriers, including fourteen different genotypes. Among them, 445 Hb QS heterozygotes showed a decrease in the mean corpuscular hemoglobin (MCH), 16 compound heterozygotes for Hb QS/α+-thal presented mild thalassemia, 28 Hb QS in combination with --SEA/αα manifested as Hb H disease, varying clinical symptoms from only moderate anemia to severe anemia and requiring blood transfusion, and 29 double heterozygotes for Hb QS and β-thal behaved as β-thal trait. The mean corpuscular volume (MCV) and MCH were significantly reduced and no Hb H peak could be detected in one patient with Hb H-Hb QS and β-thal. Meanwhile, we identified two homozygous Hb QS carriers, who showed mild to moderate anemia and increased Hb A2 level but negative results from a sequencing analysis for the first time. Additionally, Comparison of hematological parameters among the major four genotype groups showed significant differences in most box-whisker plots. People who originated from Huizhou city showed many genotypes and diversity in the clinical manifestations of Hb QS carriers. This study enlarges the mutation spectrum of α-thal and emphasizes that reliable detection of the gene mutations is important for genetic counseling. It also strengthens the prevention and control of thalassemia.

Comparison of Hematological Parameters in Ectopic Pregnancies Receiving Surgical and Medical Treatment

Comparison of Hematological Parameters in Ectopic Pregnancies Receiving Surgical and Medical Treatment

Comparison of Hematological Parameters between Diagnostic Radiation Workers and Non-Radiation Workers

Purpose: Radiation workers are exposed to chronic effects due to long-term exposure to low levels of ionizing radiation. One of the biological indicators to evaluate these chronic radiation effects is the study of the hematopoietic system. The purpose of this study is to compare the changes in blood parameters of radiology staff and staff of other wards of the hospital.&#x0D; Materials and Methods: In this study, 47 radiology staff working in the radiology department of the hospital as the study group and 94 personnel in other departments as the control group were included. Demographic data including age, gender, and work experience were obtained. Hematological parameters were extracted from the complete blood count tests of participants.&#x0D; Results: The results of this study showed that the Mean Corpuscular Hemoglobin (MCH) and Mean Corpuscular Hemoglobin Concentration (MCHC) parameters significantly decreased in the male study group, whereas no significant differences were observed in the blood parameters of the female study group in comparison to those of the control group. There was a significant positive correlation between the parameters of Hemoglobin and age. There was a significant negative correlation between work experience and white blood cells, while there was a significant positive correlation between work experience and Hemoglobin.&#x0D; Conclusion: Correlation between collective absorbed dose and changes in blood parameters is suggested for future studies. Based on the results of this study and other studies, it can be concluded that long-term exposure to low levels of radiation may change blood parameters. Therefore, regular and periodic complete blood count tests along with absorbed dose monitoring of radiation workers are recommended.

Comparison of hematological parameters between type 2 diabetes mellitus patients and healthy controls at Dessie comprehensive specialized hospital, Northeast Ethiopia: Comparative cross-sectional study.

Diabetes mellitus (DM) is a chronic condition associated with raised levels of blood glucose due to the body cannot produce any or enough insulin hormone or cannot be effectively utilized the produced insulin by the body. Patients with poorly controlled diabetes show a significant alteration in various parameters including metabolic, cellular, immunological, and hematological disturbances that leads to vascular complications. Thus, the main aim of this study is to compare hematological parameters between type 2 diabetes mellitus (T2DM) patients and healthy controls. A comparative cross-sectional study was conducted in Dessie comprehensive specialized hospital from January to June 2021. A total of 240 study participants consisting of 120 T2DM patients and 120 healthy controls were recruited using a systematic random sampling technique. Hematological parameters were determined using the DIRUI BF6500 automated hematology analyzer. Independent T-test was used to compare the mean of hematological parameters between T2DM patients and healthy controls. Pearson correlation test was used to determine the correlation between FBG, BMI, SBP, DBP, and hematological parameters in T2DM patients. Multivariate logistic regression was used to assess the association between socio-demographic and clinical variables with anemia. The result was expressed in mean and standard deviation and presented in texts and tables. P-value < 0.05 was considered to be statistically significant. The mean and standard deviation of monocyte count, basophil count, monocyte %, basophil %, RBC count, hematocrit, MCV, MCH, RDW-SD, MPV, PDW, PLC-R, and plateletcrit showed a significant difference between T2DM patients and healthy control group. Pearson correlation coefficient showed that the total WBC count, neutrophil count, monocyte count, basophil count, RDW-CV, PDW, MPV, PLC-R, and plateletcrit were statistically positively correlated with FBG whereas RBC count, Hgb, hematocrit, MCV, MCH, and RDW-SD were statistically negatively correlated with FBG in T2DM patients. Moreover, total WBC count, neutrophil count, monocyte count, basophil count, Hgb, and plateletcrit were statistically positively correlated with BMI while RBC count, Hgb, hematocrit, MCV, MCH, and RDW-SD were statistically negatively correlated with BMI in T2DM patients. On the other hand, DBP was significantly positively correlated with platelet count and RDW-CV whereas SBP also significantly positively correlated with total WBC count, neutrophil count, basophil count, and PDW. Besides, DBP and SBP showed statistically significant negative correlations with RBC count, Hgb level, and Hct value in T2DM patients. The overall prevalence of anemia was 25.8% in T2DM patients with a higher prevalence of anemia (16.7%) in female patients. Multivariate logistic regression revealed that being non-employee worker (AOR: 3.6, 95% CI, 1.4-46.0, P = 0.002), presence of neuropathy (AOR: 13.40, 95% CI, 6.83-26.28, P = 0.00), and duration of the disease ≥ 5 years (AOR = 3.2, 95% CI, 1.2-15.3, P = 0.03) have had statistically significant association with anemia inT2DM patients. Patients with T2DM may have significant alterations in various hematological parameters. Hematological parameters should be regularly tested for early diagnosis and proper management of diabetes-related complications.

Clinical parameters of hypervirulent Klebsiella pneumoniae disease and ivermectin treatment in New Zealand sea lion (Phocarctos hookeri) pups.

Hypervirulent Klebsiella pneumoniae infection causes significant mortality of endangered New Zealand sea lion pups at Enderby Island, Auckland Islands. Gross necropsy and histopathology findings are well reported, but little is known about the clinical course of disease in affected pups. To determine factors feasible as clinical screening tools for hypervirulent K. pneumoniae in live pups, 150 pups over two field seasons (2016–18) were recruited shortly after birth for a prospective cohort study. A randomised controlled clinical treatment trial with the anthelmintic ivermectin was conducted concurrently and risk factor data and biological samples were collected approximately fortnightly. Treatment with ivermectin has been demonstrated to reduce the risk of hypervirulent K. pneumoniae mortality in pups, so effects on clinical parameters between the treated and control cohorts were also investigated. A broader sample of pups were monitored for clinical signs to investigate the course of disease in affected pups. Clinical signs, haematology and oral and rectal swabs to detect gastrointestinal carriage of hypervirulent K. pneumoniae were not useful for detection of disease prior to death. Of those pups that died due to hypervirulent K. pneumoniae, only 26.1% (18/69) had any clinical signs prior, likely a reflection of the peracute course of disease. On comparison of haematological parameters between ivermectin-treated and control pups, significantly lower total plasma protein and higher eosinophil counts were seen in control versus treated pups, however standard length as a surrogate for age was a more important influence on parameters overall than ivermectin treatment. This study also highlighted a cohort of pups with severe clinical signs suggestive of hypervirulent K. pneumoniae infection were lost to follow up at the end of the monitored season, which could be contributing to cryptic juvenile mortality.

A Comparison of Haematological Parameters of Obese Individual and Non-Obese at Omisanjana Area of Ado Ekiti, Ekiti State, Nigeria

Background: The global prevalence of obesity has been on the increase over the years constituting a major public health crisis.&#x0D; Aim: The study was done to evaluate the haematological parameters of obese and non obese individuals at Omisanjana area of Ado Ekiti, Ekiti State. Nigeria.&#x0D; Materials and Methods: The study is a hospital based cross-sectional study among obese individual and non-obese individuals. This study was carried out at Omisanjana area of Ado Ekiti, Ekiti State. Fifty (50) obese individuals and fifty (50) apparently non-obese individuals were recruited as controls and enrolled in this study.&#x0D; Results: The results showed significant difference of MXD (p=0.017), haemoglobin (p=0.000), MCV (p=0.006), MCH (p=0.006), MCHC (p=0.008) and no significant difference in PCV (p=0.064), WBC (p=0.896), LYM (p=0.069), GRAN (p=0.488), RBC (p=0.820), HCT (p=0.061), PLT (p=0.819),&#x0D; when compared between obese individuals and non-obese individuals respectively.&#x0D; Conclusion: Subjects with obesity had lower mean haematological parameters such as haemoglobin mean cell volume, mean cell haemoglobin, mean cell haemoglobin concentration, neutrophils values compared to non-obese subjects. While a great majority of the parameters were of normal range for the subjects some were decreased e.g. total WBC count, Platelet and PCV.

Comparison of hematological parameters between single and twin pregnancies in Dorper ewes during gestation, lambing, and postpartum

ABSTRACT: The hematopoietic system changes during the pregnancy to carry fetal development and maternal needs. This study compared the hematological parameters between ewes with single and twin pregnancies during gestation, delivery, and postpartum. The experiment was conducted on 60 healthy pregnant Dorper ewes that were divided into two experimental groups: Group 1 (G1), with single pregnancies (n=30), and Group 2 (G2), with twin pregnancies (n=30). Blood samples were collected from all ewes at different times: immediately before fixed-time artificial insemination (AI); on day 30, 90, 120, 130, and 140 of pregnancy; immediately after delivery; and at 24h and 48h postpartum. Statistical analysis compared the two groups at different times (P&lt;0.05). Mild, normocytic, and hypochromic anemia was detected in all ewes from AI time and throughout pregnancy from both groups, but did not prove to be of clinical relevance. In the peripartum stage (from the 140th day of pregnancy to 48h postpartum), the ewes with twin pregnancies (G2) exhibited higher erythrogram values and neutrophil:lymphocyte ratio than did ewes with single pregnancies (G1). This indicated a greater hematopoietic adaptation in the body during the development of two fetuses. Except for the eosinophil numbers, all leukogram parameters were influenced by pregnancy in a similar way in both groups, and was characterized mainly by leukocytosis with neutrophilia during peripartum due to the high presence of endogenous cortisol at delivery. Thus, these findings showed that pregnancy was a stressful physiological event that increased the leukocyte count with a slight alteration in the erythrogram of Dorper ewes.

Comparison of hematological parameters in mild and severe COVID-19 infected patients: A retrospective observational study

Introduction and Aim: With the coronavirus disease 2019 (COVID-19) pandemic raging on, there is a need to identify clinical and laboratory predictors which predict progression towards severe and fatal forms of this illness. Our study aims to evaluate the ability of hematologic and biochemical biomarkers to discriminate between patients with and without severe or fatal forms of COVID-19. Materials and Methods: A retrospective study was conducted on 200 Covid positive patients;100 with mild disease and 100 with severe disease. Medical records were reviewed to collect demographic data and results of the following blood investigations were noted at admission: Hb, Platelet count, Total and Differential leukocyte count, CRP, AST, ALT, LDH, Ferritin and D-Dimer. Comparative analysis was performed between the 2 groups. Results: A significant difference in the basophil count (mean 2.35 and 5.92) among those with mild and severe disease respectively was noted as also with the eosinophil count (mean 6.88 and 1.79). The levels of CRP were higher in those with severe disease as compared to the mild disease group (mean 276.29 and 65.23). Ferritin levels were markedly increased severe disease patients (mean 1275.66 and 533.94). D-dimer was markedly increased in COVID-19 patients with severe disease (mean 3813.91 ng/ml) compared to those with mild disease group (mean 521.78 ng/ml). Conclusion: Hematological and biochemical markers positively correlate to the severity of covid infection, thus highlighting their role in the early diagnosis of the disease and can act as independent markers in predicting severity and prognosis of disease.

Reference Intervals in Malaysia: A Performance Evaluation and Comparison of Haematological Parameters between Sysmex Xe-5000 and Xn-3000

The Sysmex XN-3000 is a new automated haematology analyser designed to improve the accuracy of cell counts and the specificity of the flagging events of unusual parameters. By comparing the previous full blood count (FBC) reference intervals in Malaysia for Sysmex XE-5000, we determined a reference interval for all parameters measured by the Sysmex XN-3000 for the Malaysian population.

Pertussis and pertussis-like syndrome in young children

The most common infectious diseases with a risk of severe, non-smooth course and unfavorable outcome in young children, including the first year of life, are pertussis and respiratory syncytial viral infection (RSV infection), often occurring with pertussis-like syndrome.Objective: to establish clinical and laboratory differential diagnostic criteria for pertussis and RSV infection in children of the first year of life.Materials and methods: A retrospective comparative study was conducted, which included patients of the first year of life (from 1 month to 11 months and 29 days), regardless of the duration of hospitalization and duration of the disease: 48 patients with pertussis and 26 with acute RSV infection.Results: The compared groups of patients were comparable in age, the number of severe forms (4.6 ± 0.5 months, 17% for pertussis and 4.0 ± 0.2 months, 16% for RS infection). The disease in both cases began subacute, however, with RSV infection 92% of children were hospitalized in the first 5 days from the onset of the disease, with pertussis — in the first week of the period of convulsive cough — 47.9%, in the second week — 41.7%, in the third week — 10.4%. 52% of patients with RSV infection had no fever or (in 32%) did not exceed 1—3 days. In 41.3% of patients it was subfebrile (37—38 °C), in 21.1% it was febrile (38—39 °C). In patients with pertussis the onset of the disease took place against the background of normal body temperature. In patients with RSV infection, bronchiolitis was recorded in 24% of cases, obstructive bronchitis — in 60%. Patients with pertussis, 89.6% of whom were not vaccinated and 10.4% did not complete the initial course of vaccination, had a typical paroxysmal cough with reprises. Complications in the form of respiratory rhythm disturbances were recorded in 14.6% of cases, pneumonia — in 6.3%. Comparison of hematological parameters revealed highly significant differences in the levels of leukocytosis and relative lymphocytosis (p &lt; 0.001), as well as thrombocytosis (p &lt; 0.01), with the predominance of all indicators in patients with pertussis.Conclusion. Differential diagnostic criteria for the similarity of the clinical picture may be established hematological differences: pronounced leukocytosis due to lymphocytosis, increasing in dynamics, and thrombocytosis in pertussis and normocytosis with moderate lymphocytosis, a possible tendency to thrombocytosis in severe RSV infection.

Comparison of hematological parameters in congenital hypothyroidism in neonates: A case controls study

Thyroid hormones play an important role in human metabolism. Red blood cells abnormalities are mostly associated with thyroid hormones. However, they are very rarely investigated and associated to the permanent and transient congenital hypothyroidism in Tamil Nadu’s Government Territory care centre in Chennai. In this study, an attempt was made to study the haematological status in permanent and transient hypothyroidism neonates at birth.This retrospective study included 135 subjects, among which were permanent hypothyroidism (n=9), transient hypothyroidism (n=18), and euthyroid neonates (108). This study was carried out at department of biochemistry, Regenix super speciality laboratories and sample collection were done at Government Institute of Child’s health and hospital for children and Government institute of obstetrics and Gynaecology and hospital for Women under Madras Medical college, Chennai. The haematological parameters and thyroid profile of the subjects were assessed by sysmex heamatology analyser, Roche Cobas e411 ECLIA and Neonatal screening by BIORAD QUANTASE ELISA. Mean and standard deviation, analysis of variance (two way anova) with p&amp;#60;0.05 considered as statistically significant.In this study group, we compared the homological status in these groups, permanent, transient, and euthyroid as controls neonates. We found that haematological status like Hb, RBC, MCV, and RDW were significantly increased at birth in both permanent and transient hypothyroidism were compared with euthyroid neonates, results are significant. The results reported in these are Mean ± S.D., were statistically tested by ANOVA. In permanent Congenital Hypothyroidism (CH), MCV (79.97-87.58 fl) and RDW (13.98-16.6%), whereas in Transient Congenital Hypothyroidism, MCV (72.52-87.05 fl) and RDW (13.41-15.68%), and controls MCV (78.69-82.12 fl) and RDW (12.4 – 14.3%), suggesting that these patients were at risk of anemia and other RBS abnormalities. Mean corpuscular volume is an important to view the change in RBCs destruction, production, loss and morphology.The thyroid dyshormonogenesis is frequently associated with mothers’ complications like anemia, hypo/hyperthyroid mother, siblings developmental delay, and goitre. Permanent CH is associated with serious complication with erythrocyte abnormalities. The risk of congenital hypothyroidism cloud be getting into developmental delay and mentally retarded children, Such conditions should be detect early and corrected.

To correlate clinical profile &amp; laboratory parameters with final outcome in Plasmodium vivax (Pv) and Plasmodium falciparum (Pf) malaria

A total of 230 confirmed cases of malaria were taken up for the study from the admitted patients in MGM Medical College &amp; M. Y. Hospital, of which 141 were falciparum positive, 69 were vivax positive &amp; 20 patients were positive for both Pf &amp; Pv.Comparison of duration of stay in Plasmodium falciparum and Plasmodium vivax malariaP value &amp;#60; 0.001 highly significant; &amp;#60;0.05 significant; &amp;#62;0.05 not significant, Comparison of hematological parameters in Plasmodium falciparum and Plasmodium vivax malaria P value &amp;#60; 0.001 highly significant; &amp;#60; 0.05 significant; &amp;#62; 0.05 not significant. Cerebral malaria is the most lethal entity of severe malaria and children are more prone than other susceptible groups. Encephalopathy, shock and renal failure at the time of presentation were poor prognostic factors, while anemia and thrombocytopenia were not found to be associated with adverse outcome.Thrombocytopenia is a key indicator of malaria infebrile patients. Nature of thrombocytopenia in malaria is benign, mostly recovering with antimalarials without platelet transfusions. In our study, mortality rate of malaria was found to be 4.7%. Pf was associated with higher mortality rate as compared to Pv. Complications were more common with Pf group, though they were also seen in Pv group.

Hematological parameters in children with severe acute respiratory syndrome coronavirus 2 infection

Introduction. Recently, there has been a need to use more readily available parameters to assess the severe acute respiratory syndrome coronavirus 2 infection in children. Material and Methods. A single-center retrospective study included 30 children with severe acute respiratory syndrome coronavirus 2 infection who were admitted to the Isolation Department of the Institute for Child and Youth Health Care of Vojvodina from April to September 2020. A complete blood count with differential was performed. Additionally, systemic inflammatory index, neutrophil-lymphocyte ratio and plateletlymphocyte ratio were calculated. For comparison, age- and sex-matched 30 children negative for severe acute respiratory syndrome coronavirus 2 were included in the study. Results. In the period from April to September 2020, 30 laboratory-confirmed cases of coronavirus-19, aged 0 - 17 years, were admitted to the Institute for Child and Youth Health Care of Vojvodina. The age was not a risk factor for the development of coronavirus-19 (p = 0.707; OR: 1.018; 95% CI: 0.927 - 1.119). The comparison of hematological parameters of severe acute respiratory syndrome coronavirus 2 status showed that hemoglobin concentration (p &lt; 0.01) and hematocrit (p &lt; 0.01) were lower, and the percentage of neutrophil granulocytes (p &lt; 0.05) was higher among severe acute respiratory syndrome coronavirus 2 positive children. Furthermore, it was found that some severe acute respiratory syndrome coronavirus 2 positive children had a higher (p &lt; 0.01), while others had a lower (p &lt; 0.01) percentage of lymphocytes. The systemic inflammatory index, the ratio of neutrophils to lymphocytes, and the ratio of platelets to lymphocytes, were not found to be statistically significantly different (p &gt; 0.05). Conclusion. Low hemoglobin and hematocrit levels, a high percentage of neutrophil granulocytes, and a non-physiological percentage of lymphocytes (both, low and high) may have a diagnostic significance in children with severe acute respiratory syndrome coronavirus 2 infection.

Comparison of hematological parameters among newborns with meconium stained amniotic fluid and clear amniotic fluid

Background: Meconium stained amniotic fluid is an indirect marker of fetal hypoxia. Fetal hypoxia affects bone marrow and affects production of cell lines. Indirect marker of erythropoietin activity is nucleated red blood cells count. Aim of the study is to compare hematological parameter in newborn born with meconium stained liquor and clear amniotic fluid. Also, to determine cut off levels of nucleated RBC as marker of fetal hypoxia in meconium stained amniotic fluid(MSAF).Methods: The study was conducted over a period of 2 years from January 2012 to January 2014 in Department of Pediatrics, Sri Aurobindo medical college and hospital, Indore. One hundred newborns with meconium stained amniotic fluid (study group) and one hundred newborns with clear amniotic fluids (control group) were studied in this period. Cord blood samples were collected and hematological parameters were compared.Results: Hemoglobin % and WBC counts were seen in study group in comparison to control group, which is statistically significant (P&lt;0.05). The reported NRBC/100 WBC between the two groups was statistically analyzed. The mean NRBC count in study group were 8.09±6.09 which was statistically higher than the corresponding values in control group 2.07±2.25 (P&lt;0.05).Conclusions: Study concluded that the hemoglobin levels , WBC and NRBC counts in babies with meconium stained amniotic fluid is higher than those babies delivered with clear amniotic fluid and NRBC can be taken as a surrogate marker of hypoxia in a resource limited setting.

A comprehensive comparison of hematological parameters among 39 common diseases

Hematological parameters have been shown to be associated with morbidity and/or mortality of various disorders such as inflammatory diseases and cancer. In this study, we performed a comprehensive comparison of hematological parameters among 39 diseases, including cancer, inflammatory, autoimmune, allergic and infectious diseases as well as some further common disorders. In total, 19,038 patients and 23,610 matched healthy controls were recruited and evaluated. Our results revealed distinct hematological profiles among disease groups in which erythrocyte-related parameters were specifically associated with cancer, neutrophil- and lymphocyte-related parameters were associated with inflammatory diseases, viral infectious diseases, cancer, autoimmune and allergic diseases and platelets-related parameters were associated with viral infectious diseases. Furthermore, both neutrophil-to-lymphocyte and platelet-to-lymphocyte ratios were significantly higher in patients with inflammatory diseases, cancer, autoimmune and allergic diseases than corresponding controls. In addition, receiver operating characteristic curve analysis showed that several hematological parameters showed good diagnostic values for cancer, inflammatory diseases, and viral infectious diseases. Therefore, our results provide a valuable resource of hematological abnormalities in common diseases.

Cellular immune responses in peripheral blood lymphocytes of Giardia infected squirrel monkey (Saimiri boliviensis boliviensis) treated with Fenbendazole.

Cellular immune responses were tested to determine the effect of fenbendazole on the function of lymphocytes from Bolivian squirrel monkeys (Samiri boliviensis boliviensis). Giardia-infected squirrel monkeys were treated with commercially available fenbendazole (FBZ)-medicated monkey chow. Immune responses were compared between historical controls (Giardia naïve, untreated with FBZ (control animals)) and Giardia-infected, FBZ-treated squirrel monkeys (study animals). Peripheral blood lymphocytes from study monkeys had significantly lower stimulation indices compared to control animals when cultured in vitro with concanavalin A (Con A) (p<0.0001), phytohaemagglutinin (PHA) (p<0.0001) and lipopolysaccharide (LPS) (p<0.0001). PBMCs were also analyzed for IFN-γ producing cells in response to stimulation with Con A, PHA, PWM, and LPS by the cytokine ELISPOT assay. Significantly higher responses to Con A- (p<0.0001), and PHA- (p<0.001) stimulated cultures from Giardia-infected and fenbendazole treated compared to controls. Flow cytometric analysis for expression of cell surface markers revealed a significant increase in B- and NKT-lymphocytes and significant decrease in CD14+CD16+ monocytes after FBZ treatment. Also, circulating plasma cytokines IFN-γ, TNF-α, IL-12p40, IL-1β, IL-10, IL-13, IL-1ra, IL-6 and IL-4 were significantly decreased after FBZ treatment. Comparison of hematologic parameters between controls and FBZ-treated squirrel monkeys revealed significantly lower numbers of total leukocytes, neutrophils, monocytes, and eosinophils compared to controls. However, erythrocyte indices (red cell count, hemoglobin and hematocrit were significantly higher in FBZ-treated monkeys. Our findings suggest that fenbendazole treatment may alter sensitive immune and molecular measures of inflammation. Postponing the experimental use of squirrel monkeys until at least 6 weeks after FBZ treatment should be considered.