Comparison Of Data Research Articles

Evolutionary trends in antifungal resistance: a meta-analysis.

A kingdom-wide the largest set of published wild-type antifungal data comparison were analyzed. Trends in resistance in taxonomic groups (monophyletic clades) can be compared with the phylogeny of the fungal kingdom, eventual relationships between fungus-drug interaction and evolution can be described.

Surgical tumour resection deregulates Hallmarks of Cancer in resected tissue and the surrounding microenvironment.

Surgery exposes tumor tissue to severe hypoxia and mechanical stress leading to rapid gene expression changes in the tumor and its microenvironment, which remain poorly characterized. We biopsied tumor and adjacent normal tissue from breast (BRC) (n=81) and head/neck squamous cancer (HNSC) patients (n=10) at the beginning (A), during (B) and end of surgery (C). Tumor/normal RNA from 46/81 breast cancer patients was subjected to mRNA-Seq using Illumina short-read technology, and from nine HNSC patients to whole transcriptome microarray with Illumina BeadArray. Pathways and genes involved in 7 of 10 known cancer hallmarks, namely, tumour promoting inflammation (TNF-A, NFK-B, IL-18 pathways), activation of invasion & migration [(various Extracellular Matrix (ECM) related pathways, cell migration)], sustained proliferative signaling (K-Ras Signaling), evasion of growth suppressors (P53 signaling, regulation of cell death), deregulating cellular energetics (response to lipid, secreted factors, adipogenesis), inducing angiogenesis (hypoxia signaling, myogenesis), and avoiding immune destruction (CTLA4 and PDL1) were significantly deregulated during surgical resection (time-points A vs B vs C). These findings were validated using NanoString assays in independent pre/intra/post-operative breast cancer samples from 48 patients. In a comparison of gene expression data from biopsy (analogous to time-point A) with surgical resection samples (analogous to time-point C) from The Cancer Genome Atlas (TCGA) study, the top deregulated genes were the same as identified in our analysis, in five of the seven studied cancer types. This study suggests that surgical extirpation deregulates the hallmarks of cancer in primary tumors and adjacent normal tissue across different cancers. Implications: Surgery deregulates hallmarks of cancer in human tissue.

FEATURES OF THE USE OF DENTAL SPLINTS FOR COMPLETE DISLOCATION OF TEETH

Among dental injuries, complete dislocation of a tooth has a prevalence of 1.2 to 14.8% in the permanent dentition, and is defined as a complete displacement of the tooth from the socket with rupture of the fibers of the periodontal ligament. Among the teeth, the upper central incisors are most often affected. Among children aged seven to eleven, boys are more susceptible to this type of injury than girls. This is mostly associated with industrial and auto traumatism and sports activities, protrusion of front teeth, open bite, etc. In such cases, the severity of the damage is higher, the more pronounced the tooth protrusion.
 The purpose of the study: comparison and analysis of clinical data on the use of rigid and semi-rigid dental splints after tooth replantation under the conditions of its complete dislocation and immobilization time.
 We conducted a retrospective study of the medical records of 65 outpatient dental patients with complete dislocation of a tooth on the basis of the Poltava Regional Center of Stomatology Dental Clinical Polyclinic.
 In 26% of cases, a semi-rigid splint was used for 7-10 days, which was fixed with composite resin and a steel wire with a diameter of 0.02 cm. With rigid fixation, a higher degree of bone tissue regeneration in the periodontal space was observed in 57.5% of cases. According to our data, it was established that in relation to dental splints, semi-rigid nylon splints were used in 62% of cases, steel wire in 9%, and restorative material in 18%. According to the received data on the processing and preparation of the tooth for replantation and immobilization, physiological solution is the most suitable medium for storage (62.4%), the ideal extraalveolar time period was 25-27 minutes (58.5%), while the teeth were in a dry place within 10 minutes after storage in physiological solution. Due to the presence of serious inflammatory root resorption, which demonstrates a connection with the periodontal tissue associated with increased tooth mobility, in 27.6% of cases an intervention regarding extraction, use of a temporary prosthesis, followed by prosthetics with adhesive materials is chosen. Autotransplantation is used in 12.9% of cases and is positioned as an alternative for replacing missing incisors. However, this method has limitations, as the root of the donor tooth must be 2/3 formed, in addition to anatomical problems, where approximately 60% of autografted teeth are dissimilar in appearance in terms of asymmetric gingival width or color mismatch.
 When evaluating pulp and periodontal tissue regeneration for permanent teeth, the best results were observed with the use of platelet-rich plasma, especially for incisors, with separation after more than 8 hours of extraoral storage of the tooth and delayed replantation. The obtained data allow us to state that several factors can influence the success of replantation: the duration of the injury, the extra-alveolar period of permanence, means of preservation, contamination, manipulation and condition of the extracted tooth, the type of splint used and the time of application.
 Conclusion. Based on a retrospective analysis of medical records and literature data, it can be stated that after replantation, the use of a splint is mandatory to ensure immobilization of the teeth in the initial period, which is necessary for the restoration of the periodontal ligament.

Evaluating Hymenoptera Venom Allergy Severity: A Data-Centric Comparison of Grading Instruments.

While a consensus seems to have been reached with regard to the definition of anaphylaxis, there is no universal instrument for scoring allergic reaction severity despite more than 30 having been proposed by the time of writing. This severely hampers comparison of data between studies. While scales have been compared with regard to their utility in grading food-related reactions, no such comparisons have been made for Hymenoptera venom-associated reactions. The study conducted a retrospective analysis to compare the severity of Hymenoptera venom allergy reactions in 104 participants with suspected Hymenoptera venom allergy. The study applied six grading instruments to each reaction, also evaluating them against the NIAID/FAAN anaphylaxis criteria. Sensitivity, specificity, and receiver operating characteristic area under the curve (AUC) for identifying anaphylaxis were calculated. Severity scales were simplified into "mild," "moderate," and "severe" categories. The most common severity grade across the five scales was determined using a custom function to establish a consensus severity grade. The most common culprit insects were honeybees (49.0%). Among the 88 participants with generalized reactions, the highest proportion had involvement of four organ systems. The scales showed high specificity for detecting anaphylaxis, especially when using higher grades of the Mueller, WAO, and Dribin scales. The diagnostic yields (AUC) varied, with the WAO scale having the highest AUC (0.94) for grades 3, 4, and 5. Spearman correlation analysis showed the strongest correlations seen between the Brown and Dribin, Ring and Messmer and Dribin, and Ring and Messmer and Reisman scales. The lowest correlations were observed with the Mueller scale when paired with the WAO, Reisman, and Dribin scales. An inter-rater reliability analysis showed substantial agreement between scales with the same number of grading levels. The agreement was highest for the Brown and Dribin scales, indicating a strong consistency in reaction severity classification across different instruments. While all instruments were effective in stratifying reactions, they showed limitations in differentiating milder phenotypes. The Brown and Dribin scales stood out for their high agreement with the consensus score and sensitivity in identifying anaphylaxis. Our findings suggest that adopting either of these scales could significantly unify the reporting of allergic reactions. We believe the format of an instrument should be tailored to its intended purpose, with clinical decision aids being simpler and research tools being more detailed.

Does erectile dysfunction predict cardiovascular risk? A cross-sectional study of clinical characteristics in patients with erectile dysfunction combined with coronary heart disease.

Erectile Dysfunction (ED) is a common sexual dysfunction in men who are unable to consistently obtain and maintain sufficient penile erection to accomplish a satisfactory sexual life. ED is currently considered to be a predictor of cardiovascular disease (CVD), but few studies have observed the association between ED and clinical features of coronary heart disease (CHD). An investigation of the association between ED and clinical characteristics of CHD was carried out using a cross-sectional study design. This cross-sectional single-center study was conducted in the Department of Cardiology and included 248 patients. Associations between patients' general information, underlying disease information, coronary heart disease information, and ED severity were statistically and analytically analyzed using SPSS 26.0 software. Patients with comparable clinical characteristics were grouped together using K-means clustering. Finally, ordered logistic regression analysis was performed for general and underlying disease information. In the comparison of general data, age, education, and weekly exercise were associated with the distribution of ED severity. In the comparison of underlying disease information, the number of underlying diseases, hypertension, diabetes, hyperlipidemia, anxiety state, and depressive state were associated with the distribution of ED severity. In the comparison of CHD information, the degree of ED severity was associated with CHD subtypes, lesion sites, number of stenoses, degree of stenosis, and interventional interventions. The time from ED to CHD onset was associated with the subtypes of CHD and the number of stenoses. We clustered the main characteristics of low-risk and high-risk patients and ordered logistic regression analysis found that BMI, smoking, alcoholism, number of underlying diseases, diabetes, anxiety state, and depression state were all risk factors for CHD severity (P < 0.05); the higher the value of the above factors, the more severe the degree of CHD. Age was a protective factor for CHD severity; the younger the patient, the lower the likelihood of myocardial infarction. ED severity and the time from ED to CHD onset may be predictive of coronary heart disease severity. Reducing smoking and alcohol consumption, maintaining a healthy body weight, and regular physical activity are important in preventing CVD in ED patients.

DYNAMICS OF PARENTS’ CONSCIOUSNESS THROUGH INVOLVEMENTIN PARTNERSHIP WITH THE SCHOOL

The article presents the research results of the pedagogical conditions that form conscious parenthood in the family’s partnership with the school. The change in the consciousness of parenthood has been proven by pedagogical means. The study involved parents with one to three school-age children. Data collection and analysis was carried out using interactive programs such as Google Forms, Excel, Stat Tech v. 3.1.6 and classical methods such as data verification, comparison, interpretation, generalization and others. The results obtained prove the dependence of parental consciousness on involvement in the educational process. This fact determines the novelty of the research.

A Comparison of Multiple DEMs and Satellite Altimetric Data in Lake Volume Monitoring

Lake volume variation is closely related to climate change and human activities, which can be monitored by multi-source remote-sensing data from space. Although there are usually two routine ways to construct the lake volume by the digital elevation model (DEM) or satellite altimetric data combined with the lake area, rarely has a comparison been made between the two methods. Therefore, we conducted a comparison between the two methods in Texas for 14 lakes with abundant validation data. First, we constructed the lake hypsometric curve by five commonly applied DEMs (SRTM, ASTER, ALOS, GMTED2010, and NED) or satellite altimetric products combined with the gauge lake area. Second, the lake volume was estimated by combining the hypsometric curve with the gauge lake area time series. Finally, the estimation error has been quantitatively calculated. The results show that the relative lake volume estimation error (rVSD) of the altimetric data (4%) is only 10–18% of that of the DEMs (22–41%), and the DEM with the highest resolution (NED) has the least rVSD with an average of 22%. Therefore, for large-scale lake monitoring, we suggest the application of satellite altimetric data with the lake area to estimate the lake volume of large lakes, and the application of high-resolution DEM with the lake area to calculate the lake volume of small lakes that are gapped by satellite altimetric data.

Comparison of clinical and laboratory data of adult patients with cutaneous IgA-vasculitis and non-IgA vasculitis.

Immune complex vasculitides may be subdivided into adult IgA small vessel vasculitis (aIgA-SVV, adult Henoch-Schönlein purpura) and non-IgA-SVV (hypersensitivity vasculitis, etc.). We aimed to evaluate clinical and laboratory parameters of inpatients fulfilling the diagnostic criteria for aIgA-SVV and non-IgA-SVV. 29 adult patients (≥ 20years) with aIgA-SVV according to the EULAR/PRINTO/PRES criteria and 53 adult patients with non-IgA-SVV according to the 2012 revised International Chapel Hill Consensus Conference Nomenclature of Vasculitides were compared with respect to a variety of clinical and laboratory parameters using uni- and multivariable statistics. Compared to aIgA-SVV patients, the platelet-to-lymphocyte ratio was significantly higher in non-IgA-SVV patients. Serum C3 levels and mean corpuscular haemoglobin concentration observed in non-IgA-SVV patients were significantly lower compared to aIgA-SVV patients. Laboratory-based evidence for proteinuria and haematuria was significantly more common in patients with aIgA SVV. Only in patients with aIgA-SVV, proteinuria and haematuria significantly correlated with systemic immune-inflammation biomarkers. In patients with aIgA-SVV, higher LDH and CRP were strong independent predictors for the presence of proteinuria and proteinuria. In patients with non-IgA-SVV, female sex was a protective factor for proteinuria, while skin lesions on the upper extremities proved to be a significant independent predictor of haematuria. We detected several clinical and laboratory differences between patients with aIgA-SVV and non-IgA-SVV. In both groups, distinct predictors for renal involvement could be observed indicating that aIgA-SVV and non-IgA-SVV are very similar conditions but do not appear to present the same entity.

(081) Enhancing Treatment for Patients with Medically Unresolved Persistent Sexual Dysfunction: What can be Learnt from Mixed Methodology Evaluation-Research?

Abstract Introduction Introduction: In adult healthcare delivery worldwide, medically unresolved persistent physical symptoms (uPPS) are of significant concern (Bermingham et al., 2010; Sumathipala, 2007), with patients often requiring an enhanced therapeutic approach (Nimnuan et al., 2001; Dwamena, et al., 2009). When routine medical care is not achieving desired patient outcomes for Unresolved Persistent Sexual Symptoms (uPSS) (erectile dysfunction (ED), rapid ejaculation (RE), genital pain and diminished sexual desire), the evidence for the best practice is limited. More research is needed in this area and whilst RCTs remain the gold standard in evidence-based medicine, there are limitations. At present there is no international consensus on the use of mixed method research parameters in studies involving patients with uPPS. Sexual Medicine teams are ideally placed to develop an enhanced approach to their own uPSS sufferers. We share our experience of an evaluation-research process and report its use, adaptations, and important findings. Objective Objectives: To present our experience of Realist Evaluation methodology in developing a cross-modality consultation model: Engaging the adult patient with uPSS. Methods Methods: Following NHS ethical approval using RAMESES standards (Wong et al., 2013), we conducted mixed method data comparisons by 3 stages: 1. A wide scoping of the literature, 1990–2014, 2. A Realist Literature Synthesis (2001–2014) of efficacy or effectiveness of Short-term Psychodynamic Psychotherapy (STPP), compared through common themes of engagement with Cognitive Behavioural Therapy (CBT) for uPPS. 3. Demographics, referral sources, length of history and clinical intervention outcomes were extracted from an established NHS psychosexual service. The comparative literature findings were used to develop a common-factor thematic analysis of 24 full transcripts from four adult prospective consecutive cases. Validated measures of wellbeing and symptoms severity with goal attainment measures supported further. Results Main Results: A synthesis of the literature from stages 1. and 2., supported by clinical experience, led to a succinct definition for the majority of adult uPPS sufferers within which the uPSS cohort is embraced. A Realist equation, ‘Context plus Mechanism = Outcomes’, developed across the case data showed the simple means of discovering the unique precipitating, predisposing, and perpetuating factors of the individual’s uPSS (an approach potentially transferrable to other uPPS). Further, the extraction of common factors perpetuating the uPSS over time plus an accessible set of collaborative principles of engagement for disabling these, substantiated the consultation model. Of the measures, personal goal attainment improvements over time in therapy were the most aligned to the transcript analyses showing improved subjective outcomes and personal satisfaction. Conclusions Conclusions: The literature synthesis revealed the paucity of high-quality mixed method research into uPPS talking therapy interventions and none for heterogenous uPSS. This was the first use of an enhanced evaluation-research methodology in this context. Due to the data emerging from the embedded cases it was possible to construct the foundational cross-modality consultation model. At the time of the study, the lack of available clinical service comparator limits the generalisation of our findings to uPPS. Our experience shows from the systematically developed outcome data that realist evaluation can be used appropriately, requiring further research. Disclosure No.

Three-dimensional deviation analysis and digital visualization of shape change before and after conservation treatment of historic kiln site

The Gangjin Celadon Kiln, after its excavation in 1982, was relocated and restored in 1987 and subjected to primary conservation treatment in 2007. However, many problems such as soil disintegration and cavitation occurred in the kiln until recently. In this study, the shape changes due to the conservation treatment in 2020, which was performed to maintain the original shape of the kiln site, were recorded via three-dimensional (3D) scanning, and numerical analysis was conducted to ensure continuous monitoring and preventive conservation. From the results of this study, the locations and ranges of shape changes before and after the conservation treatment of the kiln site were identified through root-mean-square (RMS) deviation analysis and visualization, and the ranges of reinforcement and soil mulch removal were quantified through the deviations at different points. In particular, the most noticeable shape changes occurring from the conservation treatment on the kiln site with 11.2 m long and 16.7° slope were around 15 mm, and many relative changes of 40 mm or more were also observed. In addition, a reinforcement of approximately 40 mm thickness at the least and a flattening were prominently evident on the floor of the working space; the inside of the combustion chamber was visualized with a reinforcement of at least about 50 mm. Damage caused by natural or artificial factors is expected because two extensive conservation treatments were applied in 2007 and 2020 to the kiln sites. Therefore, short-term monitoring using periodic 3D scanning and time-series data comparisons is necessary for the identification of the point of shape change and the determination of major damaged areas so that a mid- to long-term monitoring plan can be established based on the findings of such observations. In addition, predictive modeling research is mandated to detect areas in the entire kiln site that exhibit a greater probability of deterioration based on the available shape change data.

(235) False Negative Penile Duplex Sonography: Results of Cavernovenous Leakage Surgery in 41 Patients with Normal End-Diastolic Velocity. The Need for Venous Leak Hemodynamic Criteria Re-Evaluation?

Abstract Introduction In cavernovenous leakage (CVL), blood entering corpora cavernosa escapes too early due to abnormal venous network, for erections to develop or maintain. CVL diagnosis relies on persisting blood flow velocity in cavernosal arteries at the end of diastole (EDV), with a consensus 5 cm/s cut-off value at pharmacologically-challenged penile duplex sonography (PC-PDS). Objective We present clinical, hemodynamic characteristics, and response to surgery of 41 patients with severe ED resistant to PDE5Is, with EDV5 cm/s at PC-PDS, considered non pathologic, but with CVLs identified by gold standard pharmacologically-challenged penile CT-cavernography. Methods Prospectively collected data from 41 consecutive patients with ED resistant to PDE5Is, with cavernosal artery EDV5 cm/s (Group A), were compared to data from 130 patients with EDV&amp;gt;5 cm/s (Group B). CVLs in Group A were detected by PC-PDS on deep dorsal vein or penile superficial veins after intracavenosal injection of Prostaglandin E1 plus Papaverine. All patients had refused a penile implant and underwent CVL intervention consisting in open surgery and embolization during the same procedure. Inter-group comparison used a T-test of means, pre- and post-operative data comparison a paired t-test. Results Patients in Group A were younger than in Group B (mean age 38.2±14.0 and 45.9±11.6 years, respectively, p&amp;lt;.0022). Pharmacologic Erection Hardness Score (EHS) was higher (2.9±.7 vs. 2.4±.6, p&amp;lt;.0001), EDV velocity was less (1.6±2.2 vs. 15.1±2.2 cm/s, p&amp;lt;.0001) in Group A than B, respectively. Pre-operative IIEF5 score was higher in Group A than in B (13.3±5.3 and 9.4±4.8, respectively, p&amp;lt;.0181). Other pre-operative erection parameters were otherwise similar in the two groups (morning erection EHS and frequency, masturbation and sexual intercourse EHS, percentage of successful penetration (p&amp;lt;.498, .543, .682, .510, .289, respectively). Patients in Group A and B responded similarly to surgery, with a similar pharmacologic EHS increase at systematic three-month post-operative PC-PDS (0.77±.94 vs. .81±.67, p&amp;lt;.80). Mixing Group A and B 171 patients, at the end of the 29.8±20.4-month follow-up, a significant improvement on all clinical parameters was observed: IIEF-5 score had increased from 9.7±5.1 to 15.5±6.0 (p&amp;lt;.0001), clinical EHS during sexual intercourse from 2.0±.7 to 3.3±.7 (p&amp;lt;.0001), penetration success rate from 22.3±31.7 to 70.1±37.2% (p&amp;lt;.0001), mean EHS during masturbation from 2.11±.67 to 3.04±.75 (p&amp;lt;.0001), morning erection EHS from .95±1.24 to 2.12±1.37 (p&amp;lt;.0001), before and after surgery, respectively. There was no statistically significant difference between Group A and B regarding all these parameters, demonstrating similar clinical responses to surgery. Conclusions Patients with EDV5 cm/s may have CVL evidenced by PC-PDS flow analysis directly on veins, and gold standard PC-PCTC. Their erection disability is severe. They respond well to combined open and embolization surgery, a confirmation that they suffer CVL. EDV criterium misses CVL in as much as one patient out of four with actual CVL in our series. Since false negative diagnosis has severe, life-long, patient and partner implications, hemodynamic criteria for CVL diagnosis should be debated in the field of sexual medicine. Disclosure No.

CILS4NEPS – Unlocking Research Potential Through More Participants, More Schools and International Comparison: Harmonized Data for Research on Education, School-to-work Transition and Integration Processes for Adolescents in Germany, the Netherlands, Sweden and England

Abstract The CILS4NEPS project combined and harmonized panel data from the Children of Immigrants Longitudinal Survey in Four European Countries (CILS4EU) and Starting Cohort 4 of the German National Educational Panel Study (NEPS SC4). This unlocks additional research potential beyond the scope of both individual datasets by increasing sample sizes and enabling international comparisons of the NEPS data. Both, the combined dataset as well as additional material to reproduce and expand the harmonization are available to users. In this article, we will first introduce the individual datasets and their comparability and describe the steps of the harmonization process. Furthermore, we will present the comparable content between both surveys, the structure of the harmonized dataset, and weighting issues. Subsequently, we provide descriptive statistics, the analytical potential, and information on data access. Lastly, we will finish with an outlook on the continuing harmonization of CILS4EU and NEPS SC4.

Investigation of the Effect of Aerosol Deposition by Applying Electrostatic Fields

Particulate matter (PM) constitutes a significant risk factor for environmental health regarding ambient air quality. An epidemiological investigation has determined that inadequate air quality is associated with the development of lung and cardiovascular diseases, chronic ailments, respiratory infections, and a significant number of fatalities on a global scale. According to the World Health Organization (WHO), the annual mortality rate due to air pollution is around 7 million individuals. The necessity for researching air quality, climate change, and the challenges posed by particulate matter (PM) is widely recognized. While reducing ambient air quality, it is essential to consider the limitations of current technical control methods. This article focuses on developing and implementing advanced technology to mitigate particle pollution in urban environments. The comparison of empirical data and computational simulations has demonstrated the efficacy of utilizing pulsed radio waves to reduce particulate matter. The control technique exhibits a threefold increase in the rate of PM concentration reduction compared to gravity settling. The approach's efficacy was evaluated through controlled trials conducted in controlled chambers and urban environments, demonstrating up to 50% reductions. The validation of the implemented test case results of the control technology was performed using historical data while considering the existence of radio waves. The coagulation process demonstrated and verified the effectiveness of reducing particle matter. The employed methodology has been shown to encourage outcomes concerning mitigating particulate matter contamination within urban and industrial environments.

Correlation analysis between Pirani score and talo-navicular angle,calcaneo-cuboid angle and tibio-calcaneall angle of infant clubfoot under ultrasound

Objective: To explore the evaluation effect of ultrasonography and Pirani score on tarsal deformity, treatment effect and pseudo-correction of congenital clubfoot in infants and young children, and the correlation between the two methods. Methods: This is a retrospective case series study. The clinical data of 26 children (40 feet) with congenital clubfoot who were evaluated by ultrasonography in the Third Affiliated Hospital of Zhengzhou University from January 2020 to January 2023 were retrospectively collected. There were 16 males and 10 females. The age at the first ultrasound examination was (M(IQR)) 9.0 (18.0) days (range: 1 to 46 days). All patients were treated with Ponseti method by the same physician. The Pirani scores before and after treatment and at the last examination, and the talonavicular angle, calcaneocuboid angle and tibiocalcaneal angle measured by ultrasound were collected, and the treatment and follow-up were recorded. Paired sample t test, repeated measures analysis of variance or Kruskal-Wallis test were used for data comparison, and Spearman correlation analysis was used for correlation analysis. The receiver operating characteristic curve was used to calculate the efficacy of ultrasound in evaluating different Pirani scores. Results: The number of plaster fixation in 26 children was 4.0 (1.0) times (range: 2 to 8 times). The medial talonavicular angle and posterior tibiocalcaneal angle were significantly improved after treatment and at the last follow-up compared with those before treatment, and the differences were statistically significant (all P<0.01). There was no difference in lateral calcaneocuboid angle before and after treatment and at the last follow-up (F=1.971, P>0.05). Pseudo-correction occurred in 2 cases (2 feet) during the treatment, with an incidence of 5%. Correlation analysis showed that there was a moderate positive correlation between talonavicular angle and Pirani midfoot score (r=0.480, P<0.01). There was no correlation between calcaneocuboid angle and Pirani midfoot score (r=0.114, P=0.105). There was a moderate negative correlation between tibial heel angle and Pirani hindfoot score (r=-0.566, P<0.01). The cut-off point of Pirani midfoot score of 1.5 was 38.78°, the sensitivity was 0.90, the specificity was 0.56, and the area under the curve was 0.75. The cut-off value of angle was 27.51 °, the sensitivity was 0.16, the specificity was 0.92, and the area under the curve was 0.44.The cut-off points of Pirani midfoot score of 3.0 were 45.08°and 9.96°, the sensitivity was 0.94 and 0.91, the specificity was 0.37 and 0.42, and the area under the curve was 0.59 and 0.62, respectively. The cut-off values of Pirani hindfoot score of 2.0 and 3.0 were 167.46° and 160.15°, respectively. The sensitivity was 0.75 and 0.67, the specificity was 0.81 and 0.83, and the area under the curve was 0.78 and 0.71, respectively. Conclusion: Ultrasound can complement with Pirani score, visually and dynamically observe the morphology and position changes of talonavicular joint, calcaneocuboid joint and tibiotalocalcaneal joint, monitor the recovery and pseudo-correction of tarsal bones, and better evaluate the therapeutic effect.

Finite Element Model of Canine-Specific Vertebrae Incorporating Biomechanical Tissue Nonlinearity

As dogs are considered valuable members of many families, ensuring their health and well-being is essential. This study introduces a numerical nonlinear model that explores the complexities of canine vertebrae, with a specific focus on their experimentally observed mechanical properties. The model underwent rigorous testing, and its results were compared with actual data on the compression of canine lumbar vertebrae. The numerical results and experimental data comparison had a 12% RRMSE. This research enhances our understanding of canine bone health and lays the groundwork for future initiatives aimed at treating and mitigating bone-related diseases in dogs.

MRI Diagnosis of Coronary Artery Lesions in Children With Kawasaki Disease and Their Correlation With Inflammatory Factors.

Ultrasonography (US), as a routine examination for evaluating coronary artery lesions (CAL) in children with Kawasaki disease (KD), has strong subjectivity and limitations. Non-contrast enhanced coronary magnetic resonance angiography (NCE-CMRA) is sensitive and reliable in displaying the segments of coronary arteries (CA). To evaluate the CA using NCE-CMRA, to compare NCE-CMRA with US, and to assess the correlation between KD-related inflammatory factors and the occurrence of CAL. Retrospective. 61 children with KD who had undergone NCE-CMRA. Ultimately, 52 cases were included (32 males and 20 females), with an average of 5.9 ± 0.3 years old. 3-T, 3D balanced turbo field echo sequence. NCE-CMRA and US coronary visualization rates were compared in 41 children who were imaged with both techniques. Inflammatory factors were compared between CAL and normal coronary artery (NCA) subgroups. In the CAL group, correlations of these inflammatory factors with CAL parameters were investigated. Comparison between groups was performed by the two independent samples t-test; the comparison of enumeration data between groups was performed by chi-square test. Receiver operating characteristic (ROC) curve analysis was performed to determine the sensitivity of inflammatory factors for detecting CAL. The correlation between CAL and inflammatory indexes was analyzed by multiple linear regression. A P value <0.05 was considered statistically significant. NCE-CMRA visualized significantly more segments than US (76% vs. 46%). There were significant differences in PLT, CRP, ESR, and D-dimer between the CAL and NCA groups. ROC curve analysis showed that the sensitivities of these four indicators in diagnosing CAL were 39%, 44%, 72%, and 61%, respectively, at cut-off points of 562.5 × 109 /L, 48.93 mg/L, 45.5 mm/h, and 0.5 mg/L, respectively. The combination of NCE-CMRA and inflammatory factors is helpful for the early diagnosis and disease severity of CAL in children with KD. 3 TECHNICAL EFFICACY: Stage 2.

Cross-jurisdictional disaster preparedness: A Nigeria-USA data-analytical approach

Disasters pose significant challenges globally, demanding effective preparedness strategies to mitigate their impact. This study proposes a novel approach to disaster preparedness through cross-jurisdictional collaboration between Nigeria and the United States, leveraging data analytics for enhanced readiness. Nigeria, with its vulnerability to various disasters, and the United States, known for its advanced disaster management systems, offer a unique opportunity for mutual learning and collaboration. This research aims to bridge the gap in disaster preparedness between these two countries by employing a data-driven analytical framework. Through the collection, analysis, and comparison of disaster-related data from both nations, this approach seeks to identify commonalities, differences, and best practices in disaster preparedness strategies. By leveraging the strengths of both countries, such as Nigeria's community resilience and the USA's technological advancements, this approach aims to develop comprehensive disaster preparedness models tailored to the specific needs and contexts of each jurisdiction. Key components of this approach include data collection from various sources, including historical disaster records, socio-economic indicators, infrastructure data, and community resilience metrics. Advanced analytical techniques, such as machine learning algorithms and spatial analysis, will be employed to identify patterns, assess vulnerabilities, and predict potential disaster scenarios. The findings will inform the development of targeted preparedness strategies, including early warning systems, evacuation plans, resource allocation, and capacity building initiatives. Furthermore, this research emphasizes the importance of multi-stakeholder collaboration and knowledge exchange in disaster preparedness. By involving government agencies, non-governmental organizations, academic institutions, and local communities from both Nigeria and the USA, this approach aims to foster a holistic and inclusive preparedness framework. Ultimately, this study seeks to contribute to the global discourse on disaster risk reduction by demonstrating the effectiveness of cross-jurisdictional collaboration and data-driven approaches in enhancing disaster preparedness. By leveraging the strengths and experiences of Nigeria and the USA, this research aims to develop scalable and adaptable models that can be applied in diverse contexts to build resilience and mitigate the impact of disasters.

Dynamics of Chromatin Opening across Larval Development in the Urochordate Ascidian Ciona savignyi.

Ascidian larvae undergo tail elongation and notochord lumenogenesis, making them an ideal model for investigating tissue morphogenesis in embryogenesis. The cellular and mechanical mechanisms of these processes have been studied; however, the underlying molecular regulatory mechanism remains to be elucidated. In this study, assays for transposase-accessible chromatin using sequencing (ATAC-seq) and RNA sequencing (RNA-seq) were applied to investigate potential regulators of the development of ascidian Ciona savignyi larvae. Our results revealed 351 and 138 differentially accessible region genes through comparisons of ATAC-seq data between stages 21 and 24 and between stages 24 and 25, respectively. A joint analysis of RNA-seq and ATAC-seq data revealed a correlation between chromatin accessibility and gene transcription. We further verified the tissue expression patterns of 12 different genes. Among them, Cs-matrix metalloproteinase 24 (MMP24) and Cs-krüppel-like factor 5 (KLF5) were highly expressed in notochord cells. Functional assay results demonstrated that both genes are necessary for notochord lumen formation and expansion. Finally, we performed motif enrichment analysis of the differentially accessible regions in different tailbud stages and summarized the potential roles of these motif-bearing transcription factors in larval development. Overall, our study found a correlation between gene expression and chromatin accessibility and provided a vital resource for understanding the mechanisms of the development of ascidian embryos.

Correlation between Serum Levels of Progranulin and Spirometric Readings in Patients with Chronic Obstructive Pulmonary Disease

Back ground Chronic Obstructive Pulmonary Disease (COPD) is a chronic Inflammatory disease of the airways and/ or parenchyma usually characterized by progressive irreversible airflow with accompanying respiratory symptoms like dyspnea, cough, production of sputum, and/or exacerbations. The pathophysiology Of COPD involves bronchitis and /or emphysema, the effect of inflammation occurs in the lung and also have systemic effect, most common causes is tobacco smoking, occupational pollution, indoor pollution. Progranulin ( PGRN ) is defined as precursor of pleiotropic glycosylated protein ,it have significant role in process of inflammation ,angiogenesis, neoplasia ,cell development ,cell cycle , embryogenesis, wound healing ,modification of autoimmune process, highly found in several type of cells like respiratory epithelial cells. Objective: The aim is to study relationship between serum level of PGRN and spirometric readings in COPD patients. Patients and method: A case control study composed of 40 patients with COPD and 45 controls, demographic characteristic, pulmonary function test and plasma PGRN were measured and made comparison of data in cases with controls, data collected were statically analysis by SPSS. Result: This study found that serum PGRN was elevated in cases of COPD more than controls (230.52±189.01 VS 114.19±68.41 ng/ml) there is significant association between PGRN and COPD ( P value 0.006 ) ,and found that PGRN negatively associated with FEV1 % ( P value 0.04 ) Conclusions: It was concluded that PGRN may be used as blood marker that indicate severity of airflow obstruction in COPD.

On the dependence of electrical mobility on temperature, humidity and structure of alkylammonium ions

Insights into the effect of temperature (T) and relative humidity (RH) as well as structure and polarisation on ion mobility help the comparison and interpretation of mobility and mass-based data. We measured alkylammonium ions in air under different T (14 °C, 24 °C, 34 °C and 41 °C) and RH (0 %, 20 %, 40 %) conditions using two individual setups (in both cases a planar differential mobility analyser coupled with a time-of-flight mass spectrometer) and the results are in excellent agreement. Mobility increases with rising T and decreases with water vapour loading. When separating the measurement mobility by structures, clear mass dependence was observed. The measured mobilities exhibited large deviations from theoretically calculated results in dry conditions, which are possibly caused by adduct formation on the monomer ions via clustering (or reactions). This phenomenon seems to be unavoidably associated with light ions under atmospheric pressures, which is worth further exploration and bearing in mind when comparing measurements to calculations. Both methanol and oxygen (occasionally nitrogen or alkyl chain elongation) are possible candidates of the adduct. Under spherical assumption, we used the modified Mason–Schamp's approximation to link the measured mobility to the mobility equivalent diameter. The drag enhancement factor ξ and the effective gas-molecule collision diameter dg derived from our measurement data are comparable to literature values. Our data also exposed a non-linear dependence on the polarisation parameter ε*. Polarisation, ξ and dg were parameterised using linear models against ion structures, T, and RH for primary, secondary and tertiary alkylammonium ions with identical alkyl groups. Our model parametrisations predict mobilities within ±10 % deviation from the measured data. The model also has satisfying predicting power for alkylammonium ions with unidentical alkyl structures.