Comparative Approach Research Articles

Plasma Proteomics Identifies B2M as a Regulator of Pulmonary Hypertension in Heart Failure With Preserved Ejection Fraction.

Pulmonary hypertension (PH) represents an important phenotype in heart failure with preserved ejection fraction (HFpEF). However, management of PH-HFpEF is challenging because mechanisms involved in the regulation of PH-HFpEF remain unclear. We used a mass spectrometry-based comparative plasma proteomics approach as a sensitive and comprehensive hypothesis-generating discovery technique to profile proteins in patients with PH-HFpEF and control subjects. We then validated and investigated the role of one of the identified proteins using in vitro cell cultures, in vivo animal models, and independent cohort of human samples. Plasma proteomics identified high protein abundance levels of B2M (β2-microglobulin) in patients with PH-HFpEF. Interestingly, both circulating and skeletal muscle levels of B2M were increased in mice with skeletal muscle SIRT3 (sirtuin-3) deficiency or high-fat diet-induced PH-HFpEF. Plasma and muscle biopsies from a validation cohort of PH-HFpEF patients were found to have increased B2M levels, which positively correlated with disease severity, especially pulmonary capillary wedge pressure and right atrial pressure at rest. Not only did the administration of exogenous B2M promote migration/proliferation in pulmonary arterial vascular endothelial cells but it also increased PCNA (proliferating cell nuclear antigen) expression and cell proliferation in pulmonary arterial vascular smooth muscle cells. Finally, B2m deletion improved glucose intolerance, reduced pulmonary vascular remodeling, lowered PH, and attenuated RV hypertrophy in mice with high-fat diet-induced PH-HFpEF. Patients with PH-HFpEF display higher circulating and skeletal muscle expression levels of B2M, the magnitude of which correlates with disease severity. Our findings also reveal a previously unknown pathogenic role of B2M in the regulation of pulmonary vascular proliferative remodeling and PH-HFpEF. These data suggest that circulating and skeletal muscle B2M can be promising targets for the management of PH-HFpEF.

Partial-Cost Analysis and Economic Impact of Ambulatory Coronary Angioplasty in a Private Hospital in the Caribbean: Análisis Parcial de Costos e Impacto Económico de la Angioplastia Coronaria Ambulatoria en un Hospital Privado del Caribe

ObjectivesThis study aimed to assess direct costs of percutaneous coronary intervention (PCI) without hospital admission versus PCI with hospital admission longer than 24 hours in a private hospital-institutional perspective in the Dominican Republic in 2022. MethodsThis study has a comparative approach based on a prospective cross-sectional partial-cost analysis. We evaluated the direct costs of 10 patients from PCI without hospital admission approach and 10 patients from a hospital admission longer than 24 hours as a control group. We used a “first-come-first-served” approach from December 2021 to March 2022. The analysis used the electronic invoice generated for each patient. ResultsPCI without hospital admission approach represents $472.56 in patient savings, equivalent to a cost reduction of 12.5%. The subcosts analysis showed the pharmacy section as the main driver of the overall cost difference. ConclusionsPCI without hospital admission was economically cost-saving compared with the control approach in direct costs in the Dominican perspective. The economic benefit is substantial and compliments the ease of use. This analysis may lead to improvements in institutional management of resources and can potentially be adapted to other health systems in the region.

Where has all the knowledge gone? migrations from industrial to knowledge economy in textile firms

ABSTRACT Adopting a comparative international case study approach, we explore the relationship between crafts and knowledge management, focusing specifically on the transformation of knowledge in firms based in declining clusters. By analysing long-standing textile firms based in Veneto and Dundee, we investigate the question “where has all the (craft) knowledge gone” and how knowledge management practices have evolved within firms and in the overall cluster. The thematic analysis of our qualitative data shows a migration of knowledge from the industrial to the contemporary knowledge economy, identifying diverse trajectories, respectively oriented to the past and to the future. The paper proposes a framework of knowledge transformation approaches based on key differences between the clusters analysed. Building on this, we also provide practical suggestions for firms still operating in the sector and broader policy implications.

Data and Methods in Corpus Linguistics: Comparative Approaches, edited by Ole Schützler & Julia Schlüter

Data and Methods in Corpus Linguistics: Comparative Approaches, edited by Ole Schützler & Julia Schlüter

The Impact of Problem-Based Learning (PBL) Vs Conventional Teaching Method on Undergraduate Students' Attitude towards Schizophrenia: A Comparative Approach

Attitudes towards Schizophrenia is a major concern and determinant of quality of mental health care. Given that nursing students are future health care providers, it is pivotal to improve their attitudes towards individuals with Schizophrenia prior to their transition into clinical practice. Problem Based Learning (PBL) is an instructional method for active learning for nursing students to understand the concept, improve the critical thinking, develop problem solving skills and shape their attitude towards Schizophrenia. This study was aimed to compare the impact of Problem Based Learning (PBL) Vs Conventional Teaching Method (CTM) on Undergraduates Nursing Students’ Attitude towards Schizophrenia. Chennai.

Factors of Sustainable Development in the Formation of the Value of Manufacturing Companies

The relevance of the research is justified by the need to search for new factors of business value formation, which are taken into account by stakeholders when making decisions. The purpose of the article is to analyze the influence of S- and G- factors on the value formation of Russian public companies in the manufacturing sector and to substantiate the importance of disclosing the indicators of sustainable development of companies as a factor of business growth. The study uses methods and tools of correlation, causal and comparative analysis. It was found that the position occupied in the RAEX ranking by S- and G- factors and the amount of capitalization are significantly correlated for companies in the extractive and manufacturing industries. There was found no statistically significant relationship between the considered indicators in relation to the companies of the agro-industrial complex. It is confirmed that the influence of sustainable development components on the formation of the companies’ value is different depending on their industry affiliation. The necessity to take into account ESG factors in the methods of comparative and income approaches is substantiated. The directions of ESG factors accounting in the methods of comparative and income approaches are highlighted. The scientific novelty of the research is determined by the substantiation of the necessity of separate accounting of the sustainable development components in the formation and assessment of business value. The practical significance of the study is that the results obtained by the author can be used by investors, owners and practicing appraisers in making managerial decisions and assessing and determining business value.

Comparative Study of Pretreatments on Coconut Fiber for Efficient Isolation of Lignocellulosic Fractions

Pretreatment is an essential step for breaking the recalcitrant structure of lignocellulosic biomass and allowing conversion to high-value-added chemicals. In this study, coconut fiber was subjected to three pretreatment methods to compare their impacts on the biomass’s structural characteristics and their efficiency in fractionating the biomass. This comparative approach was conducted to identify mild biomass pretreatment conditions that efficiently extract lignin and recover cellulose-rich pulp for the production of bioproducts. To this end, autohydrolysis, alkaline, and organosolv pretreatments were performed under different experimental conditions, and the physicochemical properties of the samples were evaluated using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), and chemical characterization of the cellulose, hemicellulose, and lignin fractions. Therefore, efficient experimental conditions were identified to pretreat coconut fibers with an extended understanding of the methods to process lignocellulose. Great delignification efficiency and pulp yield were obtained with organosolv > alkaline extraction > autohydrolysis under the selected conditions of 2 h at 185 °C in the presence of a catalyst, namely, 0.5 M NaOH, for 2 h at 55 °C and 20 min at 195 °C, respectively. FT-IR revealed a predominance of hydroxyl groups in fibers obtained from alkaline and organosolv pretreatment, showing higher lignin degradation and cellulose concentration in these samples. TGA revealed mass loss curves with similar behaviors but different patterns and intensities, and MVE analysis showed differences on the surfaces of each sample. The comparison of experimental parameters allowed the identification of suitable conditions for each extraction method, and structural analyses identified the specific characteristics of the fibers that could be obtained according to the method used. Therefore, the results are of great importance for developing sustainable and effective industrial processes.

Regulatory histories of recently withdrawn ovarian cancer treatment indications of 3 PARP inhibitors in the US and Europe: lessons for the accelerated approval pathway.

Withdrawals of drug indications may reveal potential inadequacies in the regulatory approval processes of new drugs. Understanding potential weaknesses of the regulatory approval process is paramount given the increasing use of expedited pathways. In this paper, we focus on three poly-ADP-ribose polymerase inhibitors (olaparib, rucaparib and niraparib) for the treatment of women with heavily pretreated, recurrent ovarian cancer, which were eventually withdrawn. We use a comparative case study approach to evaluate the regulatory histories of these drug indications in the US and Europe. Two drug indications benefited from the FDA's accelerated approval pathway, which explicitly lowers the bar for evidence of efficacy at the time of approval. Following accelerated approval, manufacturers are mandated to conduct post-marketing studies to confirm clinical benefit. The FDA granted accelerated approval to olaparib and rucaparib based on data on surrogate endpoints and converted the approval to regular approval after the submission of additional data on surrogate endpoints from one of two required confirmatory trials, that is, without data on clinical benefit. Niraparib directly received regular approval based only on data on a surrogate endpoint. By contrast, the EMA granted conditional marketing authorisation to rucaparib and was quicker to restrict usage than the FDA. The regulatory histories of these drug indications highlight the need to reform the accelerated approval pathway by ensuring that post-marketing requirements are followed, and that regular approval is only based on evidence of clinical benefit.

Genomic and epidemiological evidence for the emergence of a L. infantum/L. donovani hybrid with unusual epidemiology in northern Italy.

Leishmania (L.) infantum is one of the main causative agents of animal and human leishmaniasis across many endemic areas in South America, Europe, North Africa, and Asia. Despite its clinical significance, little is known about the genetic diversity of L. infantum circulating in a given endemic area. Here, we investigate this important open question by applying a comparative genomics approach to seven L. infantum isolates from different hosts and Italian regions, including the northern part of the country (Emilia-Romagna, RER), Sicily, and Sardinia, as an initial attempt to explore the breadth of parasite genetic heterogeneity in Italy. Additionally, microsatellite analysis was carried out to compare the isolates from RER with other 70 L. infantum strains from the same region as well as 65 strains belonging to the L. donovani complex from other countries. We revealed important karyotypic instability and identified strain-specific changes in gene dosage, which affected important virulence factors such as amastins and surface antigen-like proteins. Single nucleotide polymorphism-based clustering analysis of these genomes together with over 80 publicly available L. infantum and L. donovani genomes placed the Italian isolates into three geographically distinct clusters within the Mediterranean basin and uncovered three isolates clustering with putative L. infantum/L. donovani hybrids isolated in Cyprus. As judged by microsatellite profiling, these hybrid isolates are representative of a sub-population of parasites circulating in northern Italy that preferentially infect humans but not dogs. Our results place Italy at the crossroads of L. infantum infection in the Mediterranean and call attention to the public health risk represented by the introduction of non-European Leishmania species.IMPORTANCEThis study closes important knowledge gaps with respect to Leishmania (L.) infantum genetic heterogeneity in a given endemic country, as exemplified here for Italy, and reveals genetic hybridization as a main cause for re-emerging human leishmaniasis in northern Italy. The observed high diversity of Leishmania parasites on the Italian peninsula suggests different geographical origins, with genomic adaptation to various ecologies affecting both pathogenicity and transmission potential. This is documented by the discovery of a putative L. infantum/L. donovani hybrid strain, which has been shown to preferentially infect humans but not dogs. Our results provide important information to health authorities, which need to consider the public health risk represented by the introduction of new Leishmania species into EU countries due to population displacement or travel from countries where exotic/allochthonous parasite species are endemic.

Assessment of realization of the social component of sustainable development: Experience of Russia and China

Aim. To analyze the implementation of the social pillar of sustainable development (SD) with a special focus on Russia and China, and to examine the progress made by both countries on the Sustainable Development Goals (SDGs) defined by the United Nations (UN).Objectives. To assess progress in achieving the SDGs in such areas as poverty and hunger eradication, health and education development, gender equality, reduction of inequality both domestically and globally; to compare approaches and results achieved by Russia and China in the context of the SDGs; to identify common achievements and unique challenges faced by both countries in the process of realizing the social component of SD.Methods. The analysis is based on the comparison of official data, publications and reports on Russia's and China's successes in implementing SDGs. Qualitative and quantitative research methods were used to assess the degree of goal fulfillment in both countries, and comparative and analytical approaches were applied to identify and interpret the key factors influencing successes and obstacles to achieving sustainable social development.Results. Russia and China have made significant progress in realizing some of the SDGs, including poverty reduction, improving access to quality education and health care, and promoting gender equality. However, both countries face a number of challenges. Among them are inequality and internal regional differences. The article traces the differences in the approaches and strategies of Russia and China in realizing the social component of SD.Conclusions. Russia and China have made significant progress in realizing SDGs, but they still face a number of challenges. The comparative analysis reveals both common and unique strategies and problems in the realization of the social component of SD. Further progress on the SD path will require both countries to adapt their approaches to the emerging challenges and international cooperation within the global SD framework.

Chromosome-level genome assemblies of 2 hemichordates provide new insights into deuterostome origin and chromosome evolution.

Deuterostomes are a monophyletic group of animals that includes Hemichordata, Echinodermata (together called Ambulacraria), and Chordata. The diversity of deuterostome body plans has made it challenging to reconstruct their ancestral condition and to decipher the genetic changes that drove the diversification of deuterostome lineages. Here, we generate chromosome-level genome assemblies of 2 hemichordate species, Ptychodera flava and Schizocardium californicum, and use comparative genomic approaches to infer the chromosomal architecture of the deuterostome common ancestor and delineate lineage-specific chromosomal modifications. We show that hemichordate chromosomes (1N = 23) exhibit remarkable chromosome-scale macrosynteny when compared to other deuterostomes and can be derived from 24 deuterostome ancestral linkage groups (ALGs). These deuterostome ALGs in turn match previously inferred bilaterian ALGs, consistent with a relatively short transition from the last common bilaterian ancestor to the origin of deuterostomes. Based on this deuterostome ALG complement, we deduced chromosomal rearrangement events that occurred in different lineages. For example, a fusion-with-mixing event produced an Ambulacraria-specific ALG that subsequently split into 2 chromosomes in extant hemichordates, while this homologous ALG further fused with another chromosome in sea urchins. Orthologous genes distributed in these rearranged chromosomes are enriched for functions in various developmental processes. We found that the deeply conserved Hox clusters are located in highly rearranged chromosomes and that maintenance of the clusters are likely due to lower densities of transposable elements within the clusters. We also provide evidence that the deuterostome-specific pharyngeal gene cluster was established via the combination of 3 pre-assembled microsyntenic blocks. We suggest that since chromosomal rearrangement events and formation of new gene clusters may change the regulatory controls of developmental genes, these events may have contributed to the evolution of diverse body plans among deuterostomes.

A comparative plastome approach enhances the assessment of genetic variation in the Melilotus genus

BackgroundMelilotus, a member of the Fabaceae family, is a pivotal forage crop that is extensively cultivated in livestock regions globally due to its notable productivity and ability to withstand abiotic stress. However, the genetic attributes of the chloroplast genome and the evolutionary connections among different Melilotus species remain unresolved.ResultsIn this study, we compiled the chloroplast genomes of 18 Melilotus species and performed a comprehensive comparative analysis. Through the examination of protein-coding genes, we successfully established a robust phylogenetic tree for these species. This conclusion is further supported by the phylogeny derived from single-nucleotide polymorphisms (SNPs) across the entire chloroplast genome. Notably, our findings revealed that M. infestus, M. siculus, M. sulcatus, and M. speciosus formed a distinct subgroup within the phylogenetic tree. Additionally, the chloroplast genomes of these four species exhibit two shared inversions. Moreover, inverted repeats were observed to have reemerged in six species within the IRLC. The distribution patterns of single-nucleotide polymorphisms (SNPs) and insertions/deletions (InDels) within protein-coding genes indicated that ycf1 and ycf2 accumulated nonconservative alterations during evolutionary development. Furthermore, an examination of the evolutionary rate of protein-coding genes revealed that rps18, rps7, and rpl16 underwent positive selection specifically in Melilotus.ConclusionsWe present a comparative analysis of the complete chloroplast genomes of Melilotus species. This study represents the most thorough and detailed exploration of the evolution and variability within the genus Melilotus to date. Our study provides valuable chloroplast genomic information for improving phylogenetic reconstructions and making biogeographic inferences about Melilotus and other Papilionoideae species.

IB World Schools in the lusophone world: an exploratory field study in São Paulo, Lisbon and Maputo

ABSTRACT The article provides a global comparison of IB World Schools in the lusophone space, including countries in three different world regions, in response to a lack of research on international education in Portuguese-speaking countries. Following an introduction to the literature on this topic, focusing on a widely known typology of international schools, we present the first results of three case studies of the IB school field in the metropolitan regions of São Paulo, Lisbon and Maputo based on an analysis of school websites, databases and public documents. The study is based on a global comparative approach that enables an analysis of the complex interrelation between global and local actors in education, primarily addressing the school level. We aim to provide some insights on the implementation and development of the IB World Schools in Portuguese-speaking countries, in the light of existing studies on international schools.

Utilizing a comparative approach to assess genome evolution during diploidization in Artemisia tridentata, a keystone species of western North America.

Polyploidization is often followed by diploidization. Diploidization is generally studied using synthetic polyploid lines and/or crop plants, but rarely using extant diploids or nonmodel plants such as Artemisia tridentata. This threatened western North American keystone species has a large genome compared to congeneric Artemisia species; dominated by diploid and tetraploid cytotypes, with multiple origins of tetraploids with genome size reduction. The genome of an A. tridentata sample was resequenced to study genome evolution and compared to that of A. annua, a diploid congener. Three diploid genomes of A. tridentata were compared to test for multiple diploidization events. The A. tridentata genome had many chromosomal rearrangements relative to that of A. annua, while large-scale synteny of A. tridentata chromosome 3 and A. annua chromosome 4 was conserved. The three A. tridentata genomes had similar sizes (4.19-4.2 Gbp), heterozygosity (2.24-2.25%), and sequence (98.73-99.15% similarity) across scaffolds, and in k-mer analyses, similar patterns of diploid heterozygous k-mers (AB = 41%, 47%, and 47%), triploid heterozygous k-mers (AAB = 18-21%), and tetraploid k-mers (AABB = 13-17%). Biallelic SNPs were evenly distributed across scaffolds for all individuals. Comparisons of transposable element (TE) content revealed differential enrichment of TE clades. Our findings suggest population-level TE differentiation after a shared polyploidization-to-diploidization event(s) and exemplify the complex processes of genome evolution. This research approached provides new resources for exploration of abiotic stress response, especially the roles of TEs in response pathways.

Local environment shapes milk microbiomes while evolutionary history constrains milk macronutrients in captive cercopithecine primates.

Milk is a complex biochemical fluid that includes macronutrients and microbiota, which, together, are known to facilitate infant growth, mediate the colonization of infant microbiomes, and promote immune development. Examining factors that shape milk microbiomes and milk-nutrient interplay across host taxa is critical to resolving the evolution of the milk environment. Using a comparative approach across four cercopithecine primate species housed at three facilities under similar management conditions, we test for the respective influences of the local environment (housing facility) and host species on milk (a) macronutrients (fat, sugar, and protein), (b) microbiomes (16S rRNA), and (c) predicted microbial functions. We found that milk macronutrients were structured according to host species, while milk microbiomes and predicted function were strongly shaped by the local environment and, to a lesser extent, host species. The milk microbiomes of rhesus macaques (Macaca mulatta) at two different facilities more closely resembled those of heterospecific facility-mates compared to conspecifics at a different facility. We found similar, facility-driven patterns of microbial functions linked to physiology and immune modulation, suggesting that milk microbiomes may influence infant health and development. These results provide novel insight into the complexity of milk and its potential impact on infants across species and environments.

Enforceability of agreements to mediate and mediated settlement agreements in Albania

The article aims to examine the provision of the Albanian Mediation Law that provides mediation as a condition for the admissibility of the lawsuit in court when the parties have provided for it in the contract as a preliminary condition before addressing the court. This legal provision is addressed considering the right of access to court and aims to discuss how these agreements to mediate are enforced. In addition, since enforcement of mediated settlement agreements is also a guarantee for the effectiveness and success of mediation, this article also focuses on the analysis of the legal framework and the problems related to their enforcement, which differ depending on how the parties have resorted to mediation (voluntarily, due to the contract, or by referral from the court), and on the type of disputes, where mainly problems arise with conflicts of ownership over immovable property. Besides the analysis of the Albanian Mediation Law, following a comparative approach, the article also addresses the mediation laws of some foreign countries.

Constructing the Concept of Commissioner Judge in Enforcing the Exclusionary Rules Principle in Indonesia

Pretrial aims to protect human rights from misuse of authority by law enforcement officials. The concept of commissioner judges, which aims to be a substitute for the current pretrial procedure. It is expected to achieve the objectives of criminal procedural law that respects human rights by upholding the exclusionary rules principle. This study aims to understand the role of the commissioner judge in upholding the principle of exclusionary rules to achieve a criminal legal process that respects human rights. Further, a normative approach used statutory, conceptual, and comparative legal approaches. The author tries to discover the concept of a commissioner judge upholding the exclusionary rules principle to assess the validity of evidence obtained by law enforcement officials. It is hoped that the objectives of criminal procedure law that protect and guarantee the interests of human rights can be achieved by the concept of commissioner judge.

The Community’s Understanding and Responses Regarding Land Subsidence, Waterlogging, and the Link Between the Two in Rural South-western Coastal Bangladesh

Land subsidence has triggered severe waterlogging problems in the southwestern coastal zone of Bangladesh, which affects the biodiversity as well as people’s lives, and livelihoods. The members of the affected community, however, appear to have a poor understanding of the nature of land subsidence and its consequences. This study, therefore, aims to assess the community members’ understanding of as well as the actions that they take to solve problems of this nature in the rural, southwestern coastal zone of Bangladesh. A qualitative comparative case study approach was employed in this study, where two rural communities from the southwestern coastal districts were selected as the cases. By subjecting the data to thematic analysis, the study found that, although a few civil society members and activists are, to some extent, aware of the occurrence of land subsidence and its linkage with waterlogging, however, most of the members of the communities lack adequate knowledge or an understanding about the nature, causes and consequences of the land subsidence issue and its connection to the waterlogging problem in their locality. Thus, it was found that they take no or very few measures to cope with the risk of land subsidence. The study’s findings imply that the policymakers should formulate a strategy for making the members of the community aware of the nature, causes, and consequences of land subsidence issues.

Impact of Matrix Gel Variations on Primary Culture of Microvascular Endothelial Cell Function.

The endothelium regulates crucial aspects of vascular function, including hemostasis, vasomotor tone, proliferation, immune cell adhesion, and microvascular permeability. Endothelial cells (ECs), especially in arterioles, are pivotal for flow distribution and peripheral resistance regulation. Investigating vascular endothelium physiology, particularly in microvascular ECs, demands precise isolation and culturing techniques. Freshly isolated ECs are vital for examining protein expression, ion channel behavior, and calcium dynamics. Establishing primary endothelial cell cultures is crucial for unraveling vascular functions and understanding intact microvessel endothelium roles. Despite the significance, detailed protocols and comparisons with intact vessels are scarce in microvascular research. We developed a reproducible method to isolate microvascular ECs, assessing substrate influence by cultivating cells on fibronectin and gelatin matrix gels. This comparative approach enhances our understanding of microvascular endothelial cell biology. Microvascular mesenteric ECs expressed key markers (VE-cadherin and eNOS) in both matrix gels, confirming cell culture purity. Under uncoated conditions, ECs were undetected, whereas proteins linked to smooth muscle cells and fibroblasts were evident. Examining endothelial cell (EC) physiological dynamics on distinct matrix substrates revealed comparable cell length, shape, and Ca2+ elevations in both male and female ECs on gelatin and fibronectin matrix gels. Gelatin-cultured ECs exhibited analogous membrane potential responses to acetylcholine (ACh) or adenosine triphosphate (ATP), contrasting with their fibronectin-cultured counterparts. In the absence of stimulation, fibronectin-cultured ECs displayed a more depolarized resting membrane potential than gelatin-cultured ECs. Gelatin-cultured ECs demonstrated electrical behaviors akin to intact endothelium from mouse mesenteric arteries, thus advancing our understanding of endothelial cell behavior within diverse microenvironments.

Structural gendered racism as conceptualized by immigrant women in the United States

RationaleImmigrants represent a rapidly growing proportion of the population, yet the many ways in which structural inequities, including racism, xenophobia, and sexism, influence their health remains largely understudied. Perspectives from immigrant women can highlight intersectional dimensions of structural gendered racism and the ways in which racial and gender-based systems of structural oppression interact. ObjectiveThis study aims to show the multilevel manifestations of structural gendered racism in the health experiences of immigrant women living in New York City. MethodSemi-structured, in-depth interviews were conducted in 2020 and 2021 with 44 cisgender immigrant women from different national origins in New York City to explore how immigrant women experienced structural gendered racism and its pathways to their health. Interviews were thematically analyzed using a constant comparative approach. ResultsParticipants expressed intersectional dimensions of structural gendered racism and the anti-immigrant climate through restrictive immigration policy and issues related to citizenship status, disproportionate immigration enforcement and criminalization, economic exploitation, and gendered interpersonal racism experienced across a range of systems and contexts. Participants weighed their concerns for safety and facing racism as part of their life course and health decisions for themselves and their families. ConclusionsThe perspectives and experiences of immigrant women are key to identifying multilevel solutions for the burdens of structural gendered racism, particularly among individuals and communities of non-U.S. national origin. Understanding how racism, sexism, xenophobia, and intersecting systems of oppression impact immigrant women is critical for advancing health equity.