It takes 5–6 weeks to complete callus formation over callus surface in peach. During the course of investigations effect of explants collection season, type and length of explants for callusing in peach were standardized. Among various combinations best response in terms of maximum callus induction (97.78%) was noted in spring x internodal segments x 0.5 cm length of explants closely followed by (95.55%) in spring x leaf disc x 0.5 cm length of explants however maximum callus culture survival (100%) was noted in spring x leaf disc as well as in internodal segments x 0.5 cm length of explants. Observations on callus morphology indicate that the leaf disc explants collected during winter season formed white, watery type callus with poor callus growth while no callus formation was noticed in internodal segments in winter season. However, yellowish green, friable type callus with excellent growth was observed during spring season in leaf disc and green colour callus along with compact callus type were observed with internodal segment. During summer season brown colour callus with friable type were noticed in leaf disc explants with good callus growth and whitish green colour callus with semi-compact type were observed in internodal segment explants along with excellent callus growth.