Abstract
Plant biotechnology enables the production of biomass under controlled conditions, providing the synthesis of raw material in a continuous and homogeneous way. The aim of the present study was to establish Hovenia dulcis callus cultures and to evaluate their antioxidant potential in comparison with wild grown and in vitro plants. The best results for compact calli were obtained with the supplementation of 1-naphthaleneacetic acid (NAA) at 2.5 mg L-1 and the use of benzylaminopurine (BAP) enhanced callus growth. The medium supplemented with 2.5 mg L-1 NAA + 0.65 mg L-1 BAP produced 115.3±28.2 mg of dry weight. The auxin NAA was responsible for the production of light-green compact callus, while picloram and 2,4-D promoted mixed (friable and compact) calli. Total polyphenols and total flavonoids were found in higher concentrations in wild grown plants, whereas the reduction capacity and DPPH radical scavenging assays recorded higher antioxidant activity in calluses extracts. The protocols established here represent a viable and effective way for producing substances with medicinal interest. Key words: Tissue culture, total phenolics, total flavonoids, 2,2-diphenyl-1-picrylhydrazyl (DPPH), medicinal plant.
Highlights
Plant-derived natural products have been widely investigated for the discovery and development of new pharmaceuticals
Secondary metabolites are often presented in low amounts, which justify the search for alternative production methods
The purpose of this study was to evaluate the effects of plant growth regulators and explant source on the induction and establishment of H. dulcis callus cultures
Summary
Plant-derived natural products have been widely investigated for the discovery and development of new pharmaceuticals. Plant tissue culture technology provides an attractive alternative for secondary metabolite production, offering the possibility of obtaining medicinal compounds and ensuring sustainable conservation and rational use of biodiversity (Coste et al, 2011; Coppede et al, 2014). Such techniques allow controlled cultivation, providing continuous and homogeneous synthesis of raw material, regardless of environmental and seasonal factors (Praveen and Murthy, 2011). Secondary metabolites are often presented in low amounts, which justify the search for alternative production methods. Callus cultures offer a useful system for in vitro production of secondary metabolites
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