Groundwater Communities Research Articles

Response of Microbial Community Stability to Chemical Oxidation Remediation Process in a Petroleum Hydrocarbon Contaminated Groundwater Site

The stability of the microbial community is a vital indicator of microbial ecosystems. However, the mechanism of microbial community stability during in situ chemical oxidation in petroleum-hydrocarbon-polluted groundwater is unclear. This study analyzed the biomass, diversity, co-occurrence network feature and negative cohesion of microbial community at different stages to identify the changes in microbial community stability under chemical oxidation. In addition, microbial module compositions and crucial functions were analyzed to further explore the reason for the change in community stability at the module level. Multiple regression analysis was conducted to explore the microbial module explanatory degree to microbial community stability changes. The results indicated that the microbial community stability was destroyed by chemical oxidation. The carbon source effect was the main reason in the early oxidation stage, while the oxidation stress effect was the main reason in the late oxidation stage. Most microbial modules were transformed from K-strategists to r-strategists, and modular keystones were transformed to stress-tolerant species in the oxidation stage. This study suggested that microbial clusters were essential indicators of the microbial community in petroleum hydrocarbon groundwater during the chemical oxidation period.

Leachate leakage enhances the microbial diversity and richness but decreases Proteobacteria and weakens stable microbial ecosystem in landfill groundwater.

Sanitary landfill is the most prevalent and economic method for municipal solid waste disposal, and the resultant groundwater pollution has become an environmental problem due to leachate leakage. The pollution characteristics in groundwater near landfill sites have been extensively investigated, although the succession characteristics and driving mechanisms of microbial communities in leachate-contaminated groundwater and the sensitive microbial indicators for leachate leakage identification remain poorly studied. Herein, results showed that leachate leakage enhanced the microbial diversity and richness and transferred endemic bacteria from landfills into groundwater, producing an average decrease of 17.73% in the relative abundance of Proteobacteria. The key environmental factor driving the evolution of microbial communities in groundwater due to leachate pollution was organic matter, which can explain 16.13% of the changes in microbial community composition. The |βNTI| values of the bacterial communities in all six landfills were <2, and the assembly process of microbial communities was primarily dominated using stochastic processes. Leachate pollution changed the assembly mechanism, transforming the community assembly process from an undominated process to a dispersal limitation process. Leachate pollution reduced the efficiency and stability of microbial communities in groundwater, increasing the vulnerability of the stable microbial ecosystems in groundwater. Notably, microbial indicators are more sensitive to leachate leakage and could accurately identify landfills where leachate leakage occurred and other extraneous pollutants. The phylum Proteobacteria and mcrA could act as appropriate indicators for the identification of leachate leakage. These results provide a novel insight into the monitoring, identification of groundwater pollution and the scientific guidance for appropriate remediation strategies for leachate-contaminated groundwater.

Linking groundwater microbiome and functional ecological clusters to geogenic high hexavalent chromium from deep aquifers in a loess plateau

Geogenic high hexavalent chromium [Cr(Ⅵ)] in groundwater is a global environmental problem. However, the groundwater microbiome and its linkage to geogenic high Cr(Ⅵ) from deep aquifers still need to be elucidated. Here, we evaluated geogenic Cr(Ⅵ), groundwater microbiome with featured functional ecological clusters and their interactive responses in groundwater from a deep aquifer in a loess plateau of Northern Shaanxi, China. We found that the compositions and structures of microbial communities in groundwater from the deep aquifer were significantly different between low Cr(Ⅵ) (LCG, < 50 μg/L) and high Cr(Ⅵ) groundwater (HCG, > 50 μg/L), with higher microbial diversity and richness in HCG (p < 0.05). Functional “specialists” related to Cr biotransformation, including Cr(Ⅵ) reducing bacteria (CRB) Rhodococcus, Nocardioides, Novosphingobium, and Acidovorax and Mn-oxidizing bacteria (MnOB) Sphingobium, and Ralstonia were positively correlated to total Cr and Cr(VI) concentrations in groundwater. Moreover, these CRB and MnOB were dominant in high Cr(VI) groundwater and associated by strong interspecific relation in an ecological cluster (p < 0.05), suggesting their indicator roles for high Cr(Ⅵ) and the contribution of MnOB mediated Cr(III) oxidation to Cr(VI) enrichment. RDA and path analysis further revealed that the geogenic Cr(Ⅵ) directly promoted the key Cr-related functional cluster with the groundwater depth, dissolved oxygen, and total dissolved solids as the cofactors indirectly influencing Cr(Ⅵ) and the functional clusters (p < 0.05). Collectively, our results highlight the significant roles of microbial ecological clusters especially functional “specialists” MnOB and CRB in groundwater Cr(Ⅵ) from deep aquifers in the loess plateau and provide a basis for sustainable management of high Cr(Ⅵ) groundwater.

Landscape of antibiotic resistance genes and bacterial communities in groundwater on the Tibetan Plateau, and distinguishing their difference with low-altitude counterparts

Groundwater is a vital source of drinking water for Tibetans. Antibiotic resistance genes (ARGs) and bacterial communities in groundwater on the Tibetan Plateau remain unclear. Furthermore, the characterization of their differences between high-altitude and low-altitude groundwater is still unrevealed. Herein, 32 groundwater samples were collected on the plateau, and intra- and extracellular ARGs (iARGs and eARGs), and bacterial communities were characterised through qPCR assays to 19 ARGs and 16S rRNA sequencing. It showed top four abundant intra- and extracellular last-resort ARGs (LARGs) were blaOXA-48, mcr-1, vanA, and vanB, whereas dominant common ARGs (CARGs) were tetA and ermB, respectively. CARGs had higher abundances than LARGs, and iARGs were more frequently detected than eARGs. Proteobacteria, an invasive resident phylum, and Firmicutes dominated eDNA release. Network analysis revealed all observed LARGs co-occurred with pathogenic and non-pathogenic bacteria. Community diversity was significantly associated with longitude and elevation, while nitrate correlated with ARGs. Comparative analysis demonstrated eARG frequencies and abundances were higher at high altitudes than at low altitudes. Additionally, Acinetobacter and Pseudomonas specifically dominated at high altitudes. This study reveals the widespread prevalence of ARGs, particularly LARGs, in groundwater on the less-disturbed Tibetan Plateau and underlines the potential risks associated with the LARG-carrying bacteria. Environmental implicationAntibiotic resistance genes (ARGs), which are defined as emerging environmental contaminants, are becoming a global concern due to their ability to confer antibiotic resistance to pathogens. Our findings highlight the prevalence of ARGs, particularly LARGs, in groundwater on the Tibetan Plateau, and the possibility that naturally-occurring pathogenic and non-pathogenic bacteria carry multiple LARGs. In addition, we further reveal differences in the distribution of ARGs and bacterial community between high-altitude and low-altitude groundwater. Collectively, our findings offer an important insight into the potential public risks related to groundwater on the Tibetan Plateau.

Metabolic versatility enables sulfur-oxidizers to dominate primary production in groundwater

High rates of CO2 fixation and the genetic potential of various groundwater microbes for autotrophic activity have shown that primary production is an important source of organic C in groundwater ecosystems. However, the contribution of specific chemolithoautotrophic groups such as S-oxidizing bacteria (SOB) to groundwater primary production and their adaptation strategies remain largely unknown. Here, we stimulated anoxic groundwater microcosms with reduced S and sampled the microbial community after 1, 3 and 6 weeks. Genome-resolved metaproteomics was combined with 50at-% 13CO2 stable isotope probing to follow the C flux through the microbial food web and infer traits expressed by active SOB in the groundwater microcosms. Already after 7 days, 90% of the total microbial biomass C in the microcosms was replaced by CO2-derived C, increasing to 97% at the end of incubation. Stable Isotope Cluster Analysis revealed active autotrophs, characterized by a uniform 13C-incorporation of 45% in their peptides, to dominate the microbial community throughout incubation. Mixo- and heterotrophs, characterized by 10 to 40% 13C-incorporation, utilized the primarily produced organic C. Interestingly, obligate autotrophs affiliated with Sulfuricella and Sulfuritalea contained traits enabling the storage of elemental S in globules to maintain primary production under energy limitation. Others related to Sulfurimonas seemed to rapidly utilize substrates for fast proliferation, and most autotrophs further maximized their energy yield via efficient denitrification and the potential for H2 oxidation. Mixotrophic SOB, belonging to Curvibacter or Polaromonas, enhanced metabolic flexibility by using organic compounds to satisfy their C requirements. Time series data spanning eight years further revealed that key taxa of our microcosms composed up to 15% of the microbial groundwater community, demonstrating their in-situ importance. This showed that SOB, by using different metabolic strategies, are able to account for high rates of primary production in groundwater, especially at sites limited to geogenic nutrient sources. The widespread presence of SOB with traits such as S storage, H2 oxidation, and organic C utilization in many aquatic habitats further suggested that metabolic versatility governs S-fueled primary production in the environment.

May microbial ecological baseline exist in continental groundwater?

BackgroundMicrobes constitute almost the entire biological community in subsurface groundwater and play an important role in ecological evolution and global biogeochemical cycles. Ecological baseline as a fundamental reference with less human interference has been investigated in surface ecosystems such as soils, rivers, and ocean, but the existence of groundwater microbial ecological baseline (GMEB) is still an open question so far.ResultsBased on high-throughput sequencing information derived from national monitoring of 733 newly constructed wells, we find that bacterial communities in pristine groundwater exhibit a significant lateral diversity gradient and gradually approach the topsoil microbial latitudinal diversity gradient with decreasing burial depth of phreatic water. Among 74 phyla dominated by Proteobacteria in groundwater, Patescibacteria act as keystone taxa that harmonize microbes in shallower aquifers and accelerate decline in bacterial diversity with increasing well-depth. Decreasing habitat niche breadth with increasing well-depth suggests a general change in the relationship among key microbes from closer cooperation in shallow to stronger competition in deep groundwater. Unlike surface-water microbes, microbial communities in pristine groundwater are predominantly shaped by deterministic processes, potentially associated with nutrient sequestration under dark and anoxic environments in aquifers.ConclusionsBy unveiling the biogeographic patterns and mechanisms controlling the community assembly of microbes in pristine groundwater throughout China, we firstly confirm the existence of GMEB in shallower aquifers and propose Groundwater Microbial Community Index (GMCI) to evaluate anthropogenic impact, which highlights the importance of GMEB in groundwater water security and health diagnosis.ALYCGLN5KzhFnoJfDo9dQRVideo

Groundwater flow system and microbial dynamics of groundwater in a headwater catchment

Besides surface water systems, groundwater also provides a habitat for prokaryotes. The prokaryotes inhabiting groundwater are affected by the environment that they grow in. Owing to the environmental specificity, some prokaryotic community constituents might thus provide information on local subsurface growth conditions as well as advective transport processes in case of rapid dislocation. In this study, we investigated the spatiotemporal distribution of prokaryotic communities in groundwater and spring water to examine the relationship between the groundwater flow system and prokaryotic dynamics. We sampled and monitored the water in a mountainous forested catchment in Kawamata Town, Fukushima Prefecture, Japan. Seven piezometers and a spring located across a ridge, hillslope, and valley were monitored. Prokaryotic information, such as density estimated by total direct counting, community constituents and diversity using the next-generation sequencing methods, physicochemical parameters, general water quality, and groundwater mean residence time using sulfur hexafluoride (SF6) were analyzed. Our results showed that the mean residence time of groundwater and spring water ranged from less than 1 year to 30 years. The prokaryotic density ranged from 5.37 × 103cells/mL to 2.01 × 106cells/mL (n = 60). As the mean residence time increased, the density of prokaryotes in the shallow subsurface also increased, whereas that of the prokaryotes in the deep subsurface decreased. The contribution of deep groundwater to the examined groundwater is inferred using the groundwater residence time and prokaryotic information, including density and specific constituents. The stable water isotopes did not suggest the deep groundwater contribution to the groundwater at the ridge in the high-rainfall period; however, the SiO2 concentration indicated that. Elucidating the prokaryotic information along with existing hydrological tracers may enable a better understanding of groundwater flow processes.

Interaction between the hydrochemical environment, dissolved organic matter, and microbial communities in groundwater: A case study of a vegetable cultivation area in Huaibei Plain, China

Intensive vegetable planting has a profound impact on the surrounding aquatic environment. The self-purification ability of groundwater is poor, and it is difficult to return groundwater to its original state once polluted. Therefore, it is necessary to clarify the impact of intensive vegetable planting on groundwater. This study selected the groundwater of a typical intensive vegetable planting base in the Huaibei Plain of China as the research object. This work analyzed the content of major ions, the dissolved organic matter (DOM) composition, and the bacterial community structure in groundwater. Redundancy analysis was used to explore the interactions between the major ions, the DOM composition, and the microbial community. The results showed that under the influence of intensive vegetable planting, the F− and NO3−-N contents in groundwater were significantly increased; the excitation–emission matrix combined with parallel factor analysis identified four fluorescent components (C1 and C2 were humus-like components, while C3 and C4 were protein-like components), which mainly consisted of protein-like components. Proteobacteria was the dominant phylum (mean = 69.27 %), followed by Actinobacteriota (mean = 7.25 %) and Firmicutes (mean = 4.02 %), which together explained over 80 % of the total abundance; and TDS, pH, K+, and C3 were the main influencing factors affecting the microbial community structure. This study provides a better understanding of the impact of intensive vegetable cultivation on groundwater.

Next Generation Computers Warrant Next Generation Groundwater Models.

Modern hydrologic models have extraordinary capabilities for representing complex process in surface-subsurface systems. These capabilities have revolutionized the way we conceptualize flow systems, but how to represent uncertainty in simulated flow systems is not as well developed. Currently, characterizing model uncertainty can be computationally expensive, in part, because the techniques are appended to the numerical methods rather than seamlessly integrated. The next generation of computers, however, presents opportunities to reformulate the modeling problem so that the uncertainty components are handled more directly within the flow system simulation. Misconceptions about quantum computing abound and they will not be a "silver bullet" for solving all complex problems, but they might be leveraged for certain kinds of highly uncertain problems, such as groundwater (GW). The point of this issue paper is that the GW community could try to revise the foundations of our models so that the governing equations being solved are tailored specifically for quantum computers. The goal moving forward should not just be to accelerate the models we have, but also to address their deficiencies. Embedding uncertainty into the models by evolving distribution functions will make predictive GW modeling more complicated, but doing so places the problem into a complexity class that is highly efficient on quantum computing hardware. Next generation GW models could put uncertainty into the problem at the very beginning of a simulation and leave it there throughout, providing a completely new way of simulating subsurface flows.

Effects of a long-term operation wetland for wastewater treatment on the spatial pattern and function of microbial communities in groundwater

Constructed wetlands have been used globally for wastewater treatment owing to low energy inputs and operating costs. However, the impact of their long-term operation on groundwater microbial communities is still unclear. This study aims to investigate the effects and further reveal the linkage between a large-scale surface flow constructed wetland (in operation for 14 years) and groundwater. Changes in the characteristics of groundwater microbial communities and their potential influencing factors were studied based on hydrochemical analysis, Illumina MiSeq sequencing, and multivariate statistical analysis methods. Results show that the long-term operation wetland significantly elevated groundwater nutrient levels and increased the risk of ammonia nitrogen pollution compared to background values. An apparent heterogeneity of microbial communities exhibited in the vertical direction and a similarity in the horizontal direction. Wetland operations substantially altered the structure of microbial communities at 3, 5, and 12 m depths, particularly a reduced abundance of denitrifying and chemoheterotrophic functional genera. The formation and evolution of groundwater microbial community structure mainly subjected to the contributions of dissolved oxygen (33.70%), total nitrogen (21.40%), dissolved organic carbon (11.09%), and pH (10.60%) variations resulted from the wetland operation and largely differed in depths. A combined effect of these factors on the groundwater should be concerned for such a long-term running wetland system. This study provides a new insight into the responses of groundwater microbial community structure driving by wetland operation and a better understanding of corresponding variation of microbial-based geochemical processes.

From the Mountain to the Valley: Drivers of Groundwater Prokaryotic Communities along an Alpine River Corridor.

Rivers are the "tip of the iceberg", with the underlying groundwater being the unseen freshwater majority. Microbial community composition and the dynamics of shallow groundwater ecosystems are thus crucial, due to their potential impact on ecosystem processes and functioning. In early summer and late autumn, samples of river water from 14 stations and groundwater from 45 wells were analyzed along a 300 km transect of the Mur River valley, from the Austrian alps to the flats at the Slovenian border. The active and total prokaryotic communities were characterized using high-throughput gene amplicon sequencing. Key physico-chemical parameters and stress indicators were recorded. The dataset was used to challenge ecological concepts and assembly processes in shallow aquifers. The groundwater microbiome is analyzed regarding its composition, change with land use, and difference to the river. Community composition and species turnover differed significantly. At high altitudes, dispersal limitation was the main driver of groundwater community assembly, whereas in the lowland, homogeneous selection explained the larger share. Land use was a key determinant of the groundwater microbiome composition. The alpine region was more diverse and richer in prokaryotic taxa, with some early diverging archaeal lineages being highly abundant. This dataset shows a longitudinal change in prokaryotic communities that is dependent on regional differences affected by geomorphology and land use.

Occurrence of OCPs & PCBs and their effects on multitrophic biological communities in riparian groundwater of the Beiluo River, China

Persistent Organic Pollutants (POPs) may exert adverse effects on human and ecosystem health. However, as an ecologically fragile zone with strong interaction between river and groundwater, the POPs pollution in the riparian zone has received little attention. The goal of this research is to examine the concentrations, spatial distribution, potential ecological risks, and biological effects of organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) in the riparian groundwater of the Beiluo River, China. The results showed that the pollution level and ecological risk of OCPs in riparian groundwater of the Beiluo River were higher than PCBs. The presence of PCBs (Penta-CBs, Hexa-CBs) and CHLs, respectively, may have reduced the richness of bacteria (Firmicutes) and fungi (Ascomycota). Furthermore, the richness and Shannon’s diversity index of algae (Chrysophyceae and Bacillariophyta) decreased, which could be linked to the presence of OCPs (DDTs, CHLs, DRINs), and PCBs (Penta-CBs, Hepta-CBs), while for metazoans (Arthropoda) the tendency was reversed, presumably as a result of SULPHs pollution. In the network analysis, core species belonging to bacteria (Proteobacteria), fungi (Ascomycota), and algae (Bacillariophyta) played essential roles in maintaining community function. Burkholderiaceae and Bradyrhizobium can be considered biological indicators of PCBs pollution in the Beiluo River. Note that the core species of interaction network, playing a fundamental role in community interactions, are strongly affected by POPs pollutants. This work provides insights into the functions of multitrophic biological communities in maintaining the stability of riparian ecosystems through the response of core species to riparian groundwater POPs contamination.

Ultra-small bacteria and archaea exhibit genetic flexibility towards groundwater oxygen content, and adaptations for attached or planktonic lifestyles

Aquifers are populated by highly diverse microbial communities, including unusually small bacteria and archaea. The recently described Patescibacteria (or Candidate Phyla Radiation) and DPANN radiation are characterized by ultra-small cell and genomes sizes, resulting in limited metabolic capacities and probable dependency on other organisms to survive. We applied a multi-omics approach to characterize the ultra-small microbial communities over a wide range of aquifer groundwater chemistries. Results expand the known global range of these unusual organisms, demonstrate the wide geographical range of over 11,000 subsurface-adapted Patescibacteria, Dependentiae and DPANN archaea, and indicate that prokaryotes with ultra-small genomes and minimalistic metabolism are a characteristic feature of the terrestrial subsurface. Community composition and metabolic activities were largely shaped by water oxygen content, while highly site-specific relative abundance profiles were driven by a combination of groundwater physicochemistries (pH, nitrate-N, dissolved organic carbon). We provide insights into the activity of ultra-small prokaryotes with evidence that they are major contributors to groundwater community transcriptional activity. Ultra-small prokaryotes exhibited genetic flexibility with respect to groundwater oxygen content, and transcriptionally distinct responses, including proportionally greater transcription invested into amino acid and lipid metabolism and signal transduction in oxic groundwater, along with differences in taxa transcriptionally active. Those associated with sediments differed from planktonic counterparts in species composition and transcriptional activity, and exhibited metabolic adaptations reflecting a surface-associated lifestyle. Finally, results showed that groups of phylogenetically diverse ultra-small organisms co-occurred strongly across sites, indicating shared preferences for groundwater conditions.

The ecology of bacterial communities in groundwater of industrial areas: Diversity, composition, network, and assembly

Groundwater provides freshwater resources necessary for mankind, but its quality is significantly impacted by anthropologic activities. The unique characteristics of groundwater provide a special niche for bacterial colonization. To maintain the sustainability of groundwater ecosystem, a good understanding of the influencing factors and assembly mechanisms for bacterial communities is necessary. Here, we investigated the bacterial communities of groundwater from two industrial zones in Shanghai, a highly industrialized city, during the wet and dry seasons using the high-throughput sequencing technology. Our study uncovered the significant effects of season, geographical location, and industrial type on the diversity and composition of groundwater bacterial communities, particularly, we found that season was the most dominant factor with much stronger influences (give the explanation 17.7%) in comparison with geographical location (8.8%) and industrial type (7.5%). Co-occurrence networks revealed that geographical location explained more variations of bacterial ecological network than season did. Both distance-decay of similarities and variation partitioning analyses indicated that the assembly of groundwater bacterial communities was more governed by environmental filtering compared to spatial-related dispersal. Finally, null model analysis suggested the role of stochastic processes, including dispersal and drift, in shaping the groundwater bacterial communities cannot be ignored. These findings would benefit to improve our understanding of the bacterial communities in groundwater ecosystem and provide a theoretical foundation for groundwater health management.

Making the Invisible Audible: A Simple Sound-Emitting Water Level Meter.

Making the Invisible Audible: A Simple Sound-Emitting Water Level Meter.

From Recharge, to Groundwater, to Discharge Areas in Aquifer Systems in Quebec (Canada): Shaping of Microbial Diversity and Community Structure by Environmental Factors.

Groundwater recharge and discharge rates and zones are important hydrogeological characteristics of aquifer systems, yet their impact on the formation of both subterranean and surface microbiomes remains largely unknown. In this study, we used 16S rRNA gene sequencing to characterize and compare the microbial community of seven different aquifers, including the recharge and discharge areas of each system. The connectivity between subsurface and surface microbiomes was evaluated at each site, and the temporal succession of groundwater microbial communities was further assessed at one of the sites. Bacterial and archaeal community composition varied between the different sites, reflecting different geological characteristics, with communities from unconsolidated aquifers being distinct from those of consolidated aquifers. Our results also revealed very little to no contribution of surface recharge microbial communities to groundwater communities as well as little to no contribution of groundwater microbial communities to surface discharge communities. Temporal succession suggests seasonal shifts in composition for both bacterial and archaeal communities. This study demonstrates the highly diverse communities of prokaryotes living in aquifer systems, including zones of groundwater recharge and discharge, and highlights the need for further temporal studies with higher resolution to better understand the connectivity between surface and subsurface microbiomes.

Spatial distributions of microbial diversity in the contaminated deep groundwater: A case study of the Huaibei coalfield

The impact of coal mining activities on the structure of groundwater microbial communities in coal mining areas has gradually received academic attention. In this study, hydrochemical analysis and sequencing of the V4 region of the 16S rRNA gene were carried out using groundwater samples from the fourth aquifer in the loose layer of Cenozoic, the sandstone fissure aquifer in the coal measure strata of Permian, the Carboniferous Taiyuan Formation limestone aquifer, and the Ordovician limestone aquifer, at depths of 250 m, 600 m, 750 m, and 1000 m in monitoring wells. Results showed that the operational taxonomy units (OTUs) in the deep groundwater ecosystem were clustered distinguishably between the contaminated and the uncontaminated aquifers. The microbial community alpha-diversity of groundwater was significantly correlated with depth, and the richness of microbial community composition decreased with increasing depth. Proteobacteria (34.41%–97.41%), was found to be the dominant phylum, Gammaproteobacteria (10.05%–92.06%) was the dominant class and “Unassigned” (4.12%–64.72%) was dominant at the genus level. The number of endemic bacteria in the four aquifers was 1, 33, 99 and 11, respectively. It was also found that F−, oxidation-reduction potential (ORP), and TOC were the main environmental variables affecting the groundwater all OTUs, abundant OTUs, and rare OTUs, respectively. These results indicate that the activity of rare OTU subcommunities increases gradually with increasing aquifer depth and that mining significantly enriched Thiovirga in deep groundwater. In addition, it was found that with the increase of depth, the effect of ORP on microbial community abundance decreased. This study deepens our understanding of the evolution characteristics of microbial communities in deep groundwater in coal mining areas. The unique characteristics of microbial communities characteristics of four aquifers with different depths provide a microbial perspective for understanding the characteristics of deep aquifers.

Comparing the indigenous microorganism system in typical petroleum-contaminated groundwater

The environmental conditions at a contaminated site will impact on the indigenous microbial communities, with implications for the removal of pollutants. An analysis of the characteristics of microbial communities in petroleum-contaminated groundwater can give insights into the relationships between microbial community and environmental factors, and provide guidance about how microbes can be used to remediate and regulate petroleum-contaminated groundwater. This study focuses on two petroleum-contaminated sites in northeast China, the physico-chemical-biological changes in petroleum-contaminated groundwater were analyzed, the response relationship between hydro-chemical indicators and microbial communities was characterized, and the bioindicator that can reflect the petroleum contamination status were established for environmental monitoring and management. The results showed that Proteobacteria was the dominant bacteria in petroleum-contaminated groundwater, with a relative abundance of 42.45%–91.19%. pH, TDS, DO, NO3−, NO2−, SO42−, NH4+, Al, and Mn have significant effects on microbial community. The effect of petroleum pollutants on microbial communities is not only related to the concentration and composition of the pollutants themselves, but also could indirectly affect microbial communities by changing the content of inorganic electron acceptor components such as iron, manganese, sulfate and nitrate in groundwater, and this indirect effect is significantly greater than the direct impact of pollutants on microbial communities. In petroleum-contaminated groundwater, the dominant genera (Polaromonas, Caulobacter) and microbial metabolic functions (methanol oxidation, methylotrophy, ureolysis, and reductive biosynthesis) of the indigenous microbial community can be used as bioindicators to indicate petroleum contamination status. The higher abundance of these bioindicators in petroleum-contaminated groundwater, the more serious petroleum pollution in groundwater.

Seasonal context of Bristly Cave Crayfish Cambarus setosus habitat use and life history

Cave crayfishes are important members of groundwater communities, but many cave crayfishes are threatened or endangered. Unfortunately, we lack basic life history and ecological data that are needed for developing conservation plans for most cave crayfishes, especially the role of seasonal and annual fluctuations in structuring populations. Therefore, we determined the seasonal life history and habitat use of Cambarus setosus in Smallin Civil War Cave, Christian County, Missouri, United States. We conducted visual crayfish surveys over a 400 m section of the cave from 2006 to 2019. We used multinomial logit, multiple linear regression, and logistic regression models to estimate crayfish substrate, water depth, and water velocity use, respectively. All models included sex, carapace length, season, distance into the cave, and interactions between all variables and sex as predictor terms. We also used t-tests to assess morphometric differences between male and female crayfish. Six mark-recapture events (2010 to 2019) were used to estimate population sizes using a nil-recapture model. We attempted to age eight individuals using gastric mill bands, but annual bands were not discernable. We found reproductively active males during all seasons. We captured one ovigerous female during the spring, though ovigerous females were observed during show cave tours during spring, summer, and autumn. Male C. setosus were more likely to use homogenous and heterogeneous rock substrates and shallower and calmer water when compared to females; however, these relationships varied based on distance into the cave and season. Females sampled were significantly larger than males, and males regenerated chelae more often. Minimum population size estimates ranged from 9 to 159 individuals and indicated the population was relatively stable. Our data provide both a baseline population estimate for comparison with future studies and valuable trait information that is often lacking but useful for developing conservation efforts.

&lt;i&gt;Corrigendum to&lt;/i&gt;: Cryptic diversity down under: defining species in the subterranean amphipod genus &lt;i&gt;Nedsia&lt;/i&gt; Barnard &amp; Williams, 1995 (Hadzioidea: Eriopisidae) from the Pilbara, Western Australia

Amphipod crustaceans comprise a significant and enigmatic component of Australian groundwater ecosystems, particularly in the Pilbara region of Western Australia. Many amphipod species in the Pilbara, including species in the genus &lt;i&gt;Nedsia&lt;/i&gt; &lt;a href="#R1"&gt;Barnard &amp; Williams, 1995&lt;/a&gt;, are considered short range endemics, poorly or contentiously defined by taxonomic treatments based on morphology alone and have uncertain distributions as a consequence of this taxonomy. A modern systematic revision of &lt;i&gt;Nedsia&lt;/i&gt; is presented here, utilising both molecular and morphological analyses alongside distributional data to delineate species. We describe 13 new species of &lt;i&gt;Nedsia&lt;/i&gt;, confirm three existing species and synonymise eight previously described species. &lt;i&gt;Nedsia&lt;/i&gt; species are confirmed to be functionally morphologically cryptic, with &lt;i&gt;COI&lt;/i&gt; divergences at the 5&amp;#x2013;20% level. We present comparatively reduced taxonomic descriptions for these cryptic amphipod species in an effort to provide an accelerated pathway for future taxonomic work. The research provides the basis for future environmental impact assessments involving &lt;i&gt;Nedsia&lt;/i&gt; species and ongoing monitoring of the groundwater communities these form part of in the resource-rich Pilbara region.