Interaction between the hydrochemical environment, dissolved organic matter, and microbial communities in groundwater: A case study of a vegetable cultivation area in Huaibei Plain, China

Abstract

Intensive vegetable planting has a profound impact on the surrounding aquatic environment. The self-purification ability of groundwater is poor, and it is difficult to return groundwater to its original state once polluted. Therefore, it is necessary to clarify the impact of intensive vegetable planting on groundwater. This study selected the groundwater of a typical intensive vegetable planting base in the Huaibei Plain of China as the research object. This work analyzed the content of major ions, the dissolved organic matter (DOM) composition, and the bacterial community structure in groundwater. Redundancy analysis was used to explore the interactions between the major ions, the DOM composition, and the microbial community. The results showed that under the influence of intensive vegetable planting, the F− and NO3−-N contents in groundwater were significantly increased; the excitation–emission matrix combined with parallel factor analysis identified four fluorescent components (C1 and C2 were humus-like components, while C3 and C4 were protein-like components), which mainly consisted of protein-like components. Proteobacteria was the dominant phylum (mean = 69.27 %), followed by Actinobacteriota (mean = 7.25 %) and Firmicutes (mean = 4.02 %), which together explained over 80 % of the total abundance; and TDS, pH, K+, and C3 were the main influencing factors affecting the microbial community structure. This study provides a better understanding of the impact of intensive vegetable cultivation on groundwater.