Commercial Production Systems Research Articles

Crop-livestock integration provides opportunities to mitigate environmental trade-offs in transitioning smallholder agricultural systems of the Greater Mekong Subregion

CONTEXTThe Greater Mekong Subregion has been undergoing rapid agricultural transformation over the last decades, as traditional diverse subsistence-oriented agriculture is evolving towards intensified commercial production systems. Negative environmental impacts often include deforestation, nutrient pollution, and greenhouse gas (GHG) emissions. OBJECTIVEThis study aims to explore the potential of crop-livestock integration to mitigate trade-offs between economic and environmental impacts of smallholder farming systems at different stages of agricultural transition and degrees of agricultural diversity across the Greater Mekong Subregion. METHODSWe chose a ‘middle ground’ between detailed modeling of few, representative farming systems and modeling of large household populations. 24 low and high diversity farms were selected in Laos (Xieng Khouang province), Cambodia (Ratanakiri province) and Vietnam (Central Highlands) from a survey dataset of 1300 households. These farming systems were simulated with the whole-farm bio-economic and multi-objective optimization model FarmDESIGN, calculating operating profit, GHG emissions and nitrogen (N) balance. Two optimizations (‘business as usual’ vs. ‘crop-livestock integration’) were performed, generating ‘solution spaces’ or alternative configurations aiming to maximize profitability, keep farm N balanced and minimize GHG emissions. RESULTS AND CONCLUSIONSAgricultural systems across the sites differed in their production orientation and management practices, representing various stages of agricultural transition. Nitrogen balances varied between sites, being negative in Ratanakiri (average − 20.5 kg N ha−1 y−1) and Xieng Khouang (−36.5 kg N ha−1 y−1) and positive in the Central Highlands (73 kg N ha−1 y−1). Negative balances point to unsustainable mining of nutrients due to sale of cash crops without sufficient inputs, and positive balances to the risk of environmental contamination. Total GHG emissions ranged from 0.52–8.12 t CO2e ha−1 and were not significantly impacted by stage of agricultural transformation or agricultural diversity. GHG sources in Ratanakiri and Xieng Khouang were determined by crop residue burning while in Central Highlands fertilizer and livestock were main emitters. High diversity farms obtained higher operating profits (10,379 USD y−1) than low diversity farms (4584 USD y−1). Crop-livestock integration, a combination of measures including introduction of improved forages grasses, manure recycling and residue feeding, and reduction of residue burning, resulted in larger ‘solution spaces’, thus providing farmers with more options to mitigate agro-environmental trade-offs. SIGNIFICANCEThese findings underline the potential of crop-livestock integration to support sustainable intensification pathways in the Greater Mekong region. Public and private investment in further research and extension is needed to develop and scale context-specific crop-livestock integration practices.

EFFECT OF OUTDOOR ACCESS ON ETHOLOGICAL BEHAVIOR, HEALTH AND PERFORMANCE OF BROILERS IN THE TROPICAL MEXICAN CONDITIONS

<p><strong>Background. </strong>There is a concern among poultry meat consumers due to broiler conditions during rearing period in commercial production systems. Consumers trust the organic and free-range poultry production systems because they suppose are more suitable and natural, giving high nutritive value and low-fat content to the meat, improving also the chicken welfare.<strong> Objetive.</strong> Two studies evaluated the effect of an outdoor access on ethological behavior, health indicators and productive performance of Hubbard broilers in the rearing period. <strong>Methodology.</strong> The first experiment evaluated outdoor access system in spring (april to may), and the second one was implemented in summer (june to august). In both studies included two groups: a) chickens with outdoot access (OA) and b) chickens at indoor rearing only (WOA). In the first experiment, ethological behaviors (EB) were described; forage intake (Fo), feed intake (FI), live weight gain (LWG) and carcass characteristics (Cc) were measured, blood components (BC), total immunoglobulins (Ig) and parasite eggs counts in excreta (Pc) were also determined, besides microscopic gut lesions in chickens (ML) were evaluated. In the second experiment, live weight gain (LWG), feed intake (FI) and forage intake (Fo) were measured, also carcass characteristics (Cc) were determinated. Analysis of variance by one way ANOVA was performed. <strong>Results.</strong> In first experiment, it was found that OA and WOA chickens stayed the most time resting. However, inside and outside, moving and foraging behaviors were observed in OA, while in WOA treatment foraging of chickens was the less frequent conduct. WOA showed higher final live weight and FI, but there were no differences in LWG and feed conversion in comparison with OA. Gizzard and caeca weights were heavier in OA treatment. No differences in both carcass yield and abdominal fat were found. At the last two weeks of age the forage intake amount per bird was 1.93 <span style="text-decoration: underline;">+</span> 0.97 g and 2.06 <span style="text-decoration: underline;">+</span> 0.87 g of DM/d of <em>Leucaena leucocephala</em> and<em> Pennisetum purpureum</em>,<em> </em>respectively. OA chickens had fewer leukocytes number (lymphocytes, eosinophils) and total inmmunoglobulins, but more heterophils and blood hemoglobulin. No differences between treatments in <em>Eimeria</em> oocysts in excreta were found. However, higher distribution and severity in microscopic gut lesions in birds WAO treatment were found. While in second experiment it was found that both OA and WAO broilers had similar final weight, feed intake, carcass yield and abdominal fat, but OA chickens had a trend to be higher in both weight gain and better feed convertion. Likewise, AO and WAO broilers had similar tibial ash content. Also, AO broilers consumed 0.50 <span style="text-decoration: underline;">+</span> 0.36; 0.49 <span style="text-decoration: underline;">+</span> 0.50 and 0.60 <span style="text-decoration: underline;">+</span> 0.32 g of DM/d of <em>Leucaena leucocephala</em>, <em>Brosimum alicastrum</em> and <em>Moringa oleifera</em>,<em> </em>respectivily. <strong>Implications.</strong> It is basic to have the knowledge regarding poultry production with outdoor access in tropical conditions. <strong>Conclusions.</strong> Outdoor access stimulated natural behaviours expression and did not affect productive performance. Also, AO did not produce hematological changes or severe microscopic lesions.</p>

Whole Genome Sequencing of Extended-Spectrum- and AmpC- β-Lactamase-Positive Enterobacterales Isolated From Spinach Production in Gauteng Province, South Africa.

The increasing occurrence of multidrug-resistant (MDR) extended-spectrum β-lactamase- (ESBL) and/or AmpC β-lactamase- (AmpC) producing Enterobacterales in irrigation water and associated irrigated fresh produce represents risks related to the environment, food safety, and public health. In South Africa, information about the presence of ESBL/AmpC-producing Enterobacterales from non-clinical sources is limited, particularly in the water–plant-food interface. This study aimed to characterize 19 selected MDR ESBL/AmpC-producing Escherichia coli (n=3), Klebsiella pneumoniae (n=5), Serratia fonticola (n=10), and Salmonella enterica (n=1) isolates from spinach and associated irrigation water samples from two commercial spinach production systems within South Africa, using whole genome sequencing (WGS). Antibiotic resistance genes potentially encoding resistance to eight different classes were present, with blaCTX-M-15 being the dominant ESBL encoding gene and blaACT-types being the dominant AmpC encoding gene detected. A greater number of resistance genes across more antibiotic classes were seen in all the K. pneumoniae strains, compared to the other genera tested. From one farm, blaCTX-M-15-positive K. pneumoniae strains of the same sequence type 985 (ST 985) were present in spinach at harvest and retail samples after processing, suggesting successful persistence of these MDR strains. In addition, ESBL-producing K. pneumoniae ST15, an emerging high-risk clone causing nosocomical outbreaks worldwide, was isolated from irrigation water. Known resistance plasmid replicon types of Enterobacterales including IncFIB, IncFIA, IncFII, IncB/O, and IncHI1B were observed in all strains following analysis with PlasmidFinder. However, blaCTX-M-15 was the only β-lactamase resistance gene associated with plasmids (IncFII and IncFIB) in K. pneumoniae (n=4) strains. In one E. coli and five K. pneumoniae strains, integron In191 was observed. Relevant similarities to human pathogens were predicted with PathogenFinder for all 19 strains, with a confidence of 0.635–0.721 in S. fonticola, 0.852–0.931 in E. coli, 0.796–0.899 in K. pneumoniae, and 0.939 in the S. enterica strain. The presence of MDR ESBL/AmpC-producing E. coli, K. pneumoniae, S. fonticola, and S. enterica with similarities to human pathogens in the agricultural production systems reflects environmental and food contamination mediated by anthropogenic activities, contributing to the spread of antibiotic resistance genes.

Can the Caper (Capparis spinosa L.) Still Be Considered a Difficult-to-Propagate Crop?

As a perennial xerophytic shrub, characterized by plesiomorphic features, the caper (Capparis spinosa L.) is naturally spread throughout the Mediterranean basin and occupies an important ecological role, as well as an economic one, in traditional and specialized systems for commercial production. This species, in spite of its wide diffusion, is currently considered at risk of genetic erosion, mainly due to overgrazing and overharvesting for domestic uses and for trade. This situation is made more serious because of the lack of efficient propagation techniques, determining the caper as a “difficult-to-propagate species”. In this review, we report the main available sexual and vegetative propagation techniques with the aim of assessing whether, and to what extent, this criticality is still true for caper as a horticultural crop. In terms of seed propagation, germination rates have generally been considered quite low or unsatisfactory, and are also affected by hybridization phenomena that are likely to occur among both the wild and cultivated forms. The seeds show a physiological dormancy that can be lowered by adopting hormonal treatments, but in situ germination remains a critical phase. Vegetative propagation appears quite effective, mostly as related to in vitro techniques that allow caper cultivation that is no longer affected by propagation for an economic dissemination of the species in more intensive orchards. The research needs for Caper spinosa L. as a horticultural crop, especially in the field of genetic improvement and breeding, are also underlined.

Operating efficiency assessment of commercial banks with Cooperative-Stackelberg hybrid two-stage DEA

The two-stage Data Envelopment Analysis (DEA) is widely applied to assess the efficiency of commercial banks in recent years. Even though this approach well simulates the sequence of banks production process, the independent operations within sub-stages are generally ignored, and the cooperative or non-cooperative relations between sub-stages are usually investigated separately.Please check whether short title on odd pages have been set correctly. Commercial banking production system, however, has complex internal structure within which parallel and series structure can co-exist, and cooperative relations may concurrently occur with non-cooperative ones. In this paper, we develop a hybrid two-stage DEA to consider simultaneously the series-parallel internal structure and the cooperative-Stackelberg relations between sub-stages. The data of 19 Chinese listed commercial banks are used to show the abilities of the proposed models. This approach represents a powerful and flexible efficiency measurement implement that can be applied when the system in question has a complex internal structure in terms of both sub-systems features and sub-systems relations.

Keel bone fractures in Danish laying hens: Prevalence and risk factors.

Keel bone fractures (KBF) in commercial poultry production systems are a major welfare problem with possible economic consequences for the poultry industry. Recent investigations suggest that the overall situation may be worsening. Depending on the housing system, fracture prevalences exceeding 80% have been reported from different countries. No specific causes have yet been identified and this has consequently hampered risk factor identification. The objective of the current study was to investigate the prevalence of KBF in Danish layer hens and to identify risk factors in relation to KBF in all major productions systems, including parent stock production. For risk factor identification, production data from the included flocks was used. In total, 4794 birds from 40 flocks were investigated at end-of-lay. All birds were euthanized on farm and underwent inspection and palpation followed by necropsy. All observations were recorded and subsequently analysed using the SAS statistical software package. In flocks from non-caged systems, fracture prevalence in the range 53%-100%, was observed whereas the prevalence in flocks from enriched cages ranged between 50–98%. Furthermore, often multiple fractures (≥4) were observed in individual birds (range 5–81% of the birds with fractures) depending on the flock. The localization of the fractures at the distal end of the keel bone is highly consistent in all flocks (>96%). Macroscopically the fractures varied morphologically from an appearance with an almost total absence of callus, most frequently observed in caged birds, to large callus formations in and around the fracture lines, which was a typical finding in non-caged birds. Despite being housed under cage-free conditions, parent birds had significantly fewer fractures (all flocks were 60 weeks old) per bird, than other birds from cage-free systems. The body weight at end-of-lay had an effect on the risk of having fractures, heavy hens have significantly fewer fractures at end-of-lay. The older the hens were at onset of lay, the lower was the flock prevalence at end-of-lay. Additionally, the daily egg size at onset of lay was of importance for the risk of developing fractures, the production of heavier eggs initially, resulted in higher fracture prevalence at depopulation. The odds ratio of body weight, (+100 g) was 0.97, age at onset of lay (+1 week) was 0.87 and daily egg weight at onset (+1 gram) was 1.03. In conclusion, the study demonstrated a very high prevalence of KBF in hens from all production systems and identified hen size, age at onset of lay and daily egg weight at onset of lay to be major risk factors for development of KBF in the modern laying hen. Further research regarding this is warranted to strengthen the longevity and enhance the welfare of laying hens.

Integrative analysis of transcriptomic and metabolomic profiles reveal the complex molecular regulatory network of meat quality in Enshi black pigs

Improving meat quality is a crucial purpose of commercial production and breeding systems. In this study, multiomics techniques were used to investigate the molecular mechanisms that impact the excessive diversity of meat quality in Enshi black pigs. The results suggest that 120 differentially expressed genes (DEGs) and 171 significantly changed metabolites (SCMs) contribute to the content of intramuscular fat (IMF) through the processes of fat accumulation and regulation of lipolysis. A total of 141 DEGs and 47 SCMs may regulate meat color through the processes of nicotinate and nicotinamide metabolism. Herein, we found some candidate genes associated with IMF and meat color. We also presented a series of metabolites that are potentially available biological indicators to measure meat quality. This research provides further insight into the detection of intramuscular fat accumulation and meat color variation and provides a reference for molecular mechanisms in the regulation of IMF and meat color.

Comparison of UAV and fixed-wing aerial application for alfalfa insect pest control: evaluating efficacy, residues, and spray quality.

Integrating unmanned aerial vehicles (UAV) as a new method of pesticide application into existing commercial crop protection systems requires extensive research and comparison to conventional, proven application technology. Pest control expressed as efficacy against target pests, and spray quality expressed as coverage and chemical residue are three key criteria. We investigated and compared these quantitative parameters between a multi-rotor UAV and conventional piloted airplanes in two commercial alfalfa production systems. Effective and equivalent control of leaf-feeding insect pests was achieved by both methods of aerial application when delivering chlorantraniliprole at the same labeled use rate in different spray volumes (46.8 and 93.5L ha-1 ) on commercially grown alfalfa in California. Residue levels and spray coverage were also comparable and consistent between the UAV and airplane applications across three sampling techniques, specifically residue levels on alfalfa, insecticide recovery from filter paper, and spray coverage on water sensitive cards. Differences in droplet size and deposit characteristics were more variable for the UAV than airplanes based on analysis of deposition images. The results of this study provide confidence supporting the use of small-scale multi-rotor UAVs for pesticide application on agricultural crops. According to the parameters tested, UAV application quality and crop protection performance were comparable to that of the conventional fixed wing airplane application. However, the droplet spectrum and the short-term fate of droplets from unmanned aerial spray system require further optimization for effective and efficient crop protection with minimal risk to the environment.

First report of the mussel Mytella strigata (Hanley, 1843) in the Venezuelan Caribbean from an invasion in a shrimp farm

Individuals of mussels were collected in ponds from a commercial shrimp farm in the Unare region, Anzoátegui State, Venezuela. Identification was carried out using dichotomous keys and corroborated via analysis of similarities between the sequences of partial mitochondrial DNA of the cytochrome oxidase gene and Mytella strigata (Hanley, 1843) reported in GenBank with 99-100% similarity. Morphological analyses further supported the identification of the specimen. The first report of M. strigata in the Venezuelan Caribbean coast suggests that it could be a transplanted species. Possible negative effects of M. strigata on commercial shrimp production systems are discussed.

Medical Management of Milk Fever in a Crossbred Cow

Background: The livestock sector contributes nearly 17.1% of the value of output at current prices in Agriculture, Fishery and Forestry sector. The overall contribution of Livestock Sector in total GDP was nearly 5.1% during 2019-20. Dairying in India is witnessing transformation from traditional production system to either semi-commercial or commercial production system, which requires high producing dairy animals. Losses associated with milk fever are due to deaths, reduction in the productive lifespan of affected cow and reduction in milk production following each milk fever episode, as well as costs of prevention and treatment. It has been reported that about one in 20 affected cows, dies due to milk fever. Further, milk fever can contribute to dystocia, uterine prolapse, retained fetal membranes, metritis, abomasal displacement and mastitis leading to serious economic loss. Methods: A Jersey crossbred cow on its sixth parity was presented with the history indicated that the animal had calved 45 days ago and was semi-intensively managed. The daily production of milk was abou 15 liters. Physical examination revealed the temperature and pulse rate were within the normal range, but there was increased in respiratory rate and a dehydration status of 5%. The dairy cow was diagnosed with milk fever and treatment was done by slowly infusing 400 ml of Calcium borogluconate into the jugular vein over a period of 10-20 minutes. Intravenous infusion of 1000 ml 0.9% NaCl and 2000 ml of 25% glucose were instituted in order to restore blood glucose level and to correct the dehydration. Result: The animal recovered after the treatment, thus the prognosis was good and supportive therapy was continued for three days and animal had an uneventful recovery.

Review on Poultry Production Status and Economic Contribution of the Sector in Ethiopia

This review focused on the poultry production status, breed/ecotype characterization, genetic improvement trends and economic value of the poultry sector in Ethiopia. The poultry production system in Ethiopia can be classified into four categories, large scale commercial, medium scale commercial, small scale commercial and village or backyard commercial production system. Chickens play an important role in the human economy, supplying food, creating wealth through job creation for the booming population. About 78.85% of the Ethiopian poultry population consists of indigenous chickens, while the remaining 21.14% consists of exotic and hybrid breeds of chickens. The indigenous chickens of Ethiopia have various names and characterized based on their ecological or main habitat, depend on qualitative characteristics. The molecular characterizations of some Ethiopian indigenous chicken ecotype were conducted. The improvements of the productivity of indigenous chicken ecotype are mainly focused on the introduction of high yielding exotic chickens. Exotic chickens were first introduced into Ethiopia in 1953. The genetic improvement through introduction of exotic breeds was failed due to less adapted to local environment conditions, poor management; lack of input and output markets; and shortage of quality feeds, vaccines and veterinary inputs. Chickens are the backbone economy base of poor resource households in Ethiopia. The economic contribution of the poultry production sector is not still proportional to the huge chicken numbers, due to the presence of many productions, reproduction and infrastructural constraints. To use full potential of poultry sector, it requires the development of proper poultry breeding program and strategy. Keywords: breed, chicken, economy, exotic, genetic improvement, indigenous chicken, phenotypic characterization DOI: 10.7176/FSQM/109-01 Publication date: June 30 th 2021

Compact shoot architecture of Osteospermum fruticosum transformed with Rhizobium rhizogenes.

Improved compact shoot architecture of Osteospermum fruticosum Ri lines obtained through Rhizobium rhizogenes transformation reduces the need for chemical growth retardants. Compactness is for many ornamental crops an important commercial trait that is usually obtained through the application of growth retardants. Here, we have adopted a genetic strategy to introduce compactness in the perennial shrub Cape daisy (Osteospermum fruticosum Norl.). To this end, O. fruticosum was transformed using six different wild type Rhizobium rhizogenes strains. The most effective R. rhizogenes strains Arqua1 and ATCC15834 were used to create hairy root cultures from six Cape daisy genotypes. These root cultures were regenerated to produce transgenic Ri lines, which were analyzed for compactness. Ri lines displayed the characteristic Ri phenotype, i.e., reduced plant height, increased branching, shortened internodes, shortened peduncles, and smaller flowers. Evaluation of the Ri lines under commercial production conditions showed that similar compactness was obtained as the original Cape daisy genotypes treated with growth retardant. The results suggest that the use of chemical growth retardants may be omitted or reduced in commercial production systems of Cape daisy through implementation of Ri lines in future breeding programs.

Exploiting digital droplet PCR and Next Generation Sequencing technologies to determine the relative abundance of individual Eimeria species in a DNA sample

DNA-based diagnostic assays for detecting infections with Eimeria species have been limited to providing identification and presence/absence data for samples containing oocysts. Modern technologies that generate quantitative data, such as droplet digital PCR (ddPCR) and Next Generation Sequencing (NGS), utilize a relatively short amplicon size containing sufficient species-specific variation for reliable species level identification. Targeting the cytochrome c oxidase subunit III gene in the mitochondrial genome, we established protocols using these technologies to determine the relative abundance of the number of copies/μL of Eimeria species in a sample. Samples from chickens of known and unknown Eimeria species composition were analyzed to determine the suitability of these technologies as diagnostic assays. All technologies demonstrated robust capability of identifying and quantifying the Eimeria species in samples. The new quantitative assays described herein will produce invaluable detail of Eimeria species infections for an array of situations in commercial chicken production systems, enabling further characterization of the disease profile and allowing for the development or enhancement of new intervention strategies.

Longevity and lifetime reproductive trait genetic parameter estimates from Thai Landrace and Large White pig populations.

The objective of this study was to estimate genetic parameters for age at first farrowing (AFF), length of productive life (LPL), lifetime number of piglets born alive (LNBA), lifetime number of piglets weaned (LNW), lifetime wean to first service interval (LW2S), and lifetime pig efficiency (LTP365) from commercial Thai Landrace and Large White populations. Data from 12,843 Landrace (LR) and 14,757 Large White (LW) sows in a Thai commercial pork production system were utilized in this study. The genetic parameters for each trait were estimated using the average information restricted maximum likelihood algorithm where a multivariate animal model was implemented. Heritability estimates for AFF and LPL were low for LR (0.11 and 0.02, respectively) and LW (0.19 and 0.07, respectively). Heritability estimates for lifetime traits ranged from 0.01 to 0.05 for LR and 0.04 to 0.09 for LW. The genetic correlation between LPL with LNBA, LNW, LW2S and LTP365 were positive for LR (0.39 to 0.90) and LW (0.59 to 0.94). Favourable genetic correlations were observed between AFF and LPL for LR (- 0.14) and LW (- 0.14). The phenotypic correlations between LPL and lifetime traits ranged from 0.64 to 0.92 for LR and 0.61 to 0.91 for LW. Favourable phenotypic correlation between AFF and lifetime production traits was observed in the present study. The estimated heritability for the length of productive life and lifetime traits was relatively lowly heritable; length of productive life is genetically associated with lifetime traits. These findings indicate that sow lifetime productivity could be improved using selection on LPL and AFF without antagonism. Thus, improving sow lifetime productivity in the commercial pork production system, AFF and LPL should be included in a maternal line genetic evaluation programme.

Antimicrobial Use, Residues, Resistance and Governance in the Food and Agriculture Sectors, Tanzania.

All infections are potentially curable as long as the etiological agents are susceptible to antimicrobials. The increased rate at which antimicrobials are becoming ineffective is a global health risk of increasing concern that threatens withdrawal of beneficial antimicrobials for disease control. The increased demand for food of animal origin, in particular eggs, meat and milk has led to intensification and commercial production systems where excessive use and misuse of antimicrobials may prevail. Antimicrobials, handled and used by farmers and animal attendants with no formal education, may be predisposed to incorrect dosages, misuse, incorrect applications and non-adherence to withdrawal periods. This study was conducted to assess the regulatory roles and governance of antimicrobials, establish the pattern and extent of their use, evaluate the antimicrobial residues and resistance in the food animals and crop agriculture value chains, and relate these findings to existing strategies in place for combating the emergence of antimicrobial resistance in Tanzania. A multimethod approach (desk review, field study and interviews) was used. Relevant establishments were also visited. High levels of resistance to penicillin G, chloramphenicol, streptomycin and oxytetracycline have been reported, especially for Actinobacter pyogenes, Staphylococcus hyicus, Staphylococcus intermedius and Staphylococcus aureus from dairy cattle with mastitis and in humans. Similar trends were found in poultry where eggs and meat are contaminated with Escherichia coli strains resistant to amoxicillin + clavulanate, sulphamethoxazole and neomycin. An increasing trend of emerging multidrug resistant E. coli, Klebsiella pneumoniae, Staphylococcus aureus and Salmonella was also found in food animals. An increase in methicillin resistant Staphlococcus aureus (MRSA) and extended-spectrum beta-lactamase (ESBL) in the livestock sector in Tanzania have been reported. The pathogens isolated in animals were resistant to ampicillin, augmentin, gentamicin, co-trimoxazole, tetracycline, amoxicillin, streptomycin, nalidixic acid, azithromycin, chloramphenicol, tylosin, erythromycin, cefuroxime, norfloxacin and ciprofloxacin. An increased usage of antimicrobials for prophylaxis, and therapeutics against pathogens and for growth promotion in livestock, aquaculture and crop production were observed. A One Health strategic approach is advocated to combat antimicrobial resistance (AMR) in the food and agriculture sectors in Tanzania. Practical recommendations include (a) legislation review and implementation; (b) antimicrobial use (AMU), AMR and antimicrobial residue (AR) awareness and advocacy among stakeholders along the value chain; (c) strengthening of surveillance and monitoring programs for AMU, AMR and AR; (d) enhanced development and use of rapid and innovative diagnostic tests and the promotion of biosecurity principles; and (e) good husbandry practices. The utilization of this information to improve public health policies and reduce the burden of AMR will be beneficial.

Leaf Nutrient Concentrations and Dry Biomass of Fig Plants as Modified by the Application of NPK: A Preliminary Study

Aims: The effect of a complete NPK matrix on leaf nutrient concentrations and dry biomass of ‘Black Mission’ fig plant organs was tested under an intensive culture system and protected environment.
 Study Design: A randomized complete block design with four blocks was employed.
 Place and Duration of Study: The experiment was conducted from April to November 2016 at the Campo Experimental La Laguna, located in Matamoros, Coahuila, Mexico. This research station belongs to the Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP) of Mexico. The experiment was set up under a macro tunnel equipped with a shade mesh with 50% sunlight attenuation.
 Methodology: Two-year-old fig plants (cv. ‘Black Mission’) previously propagated from stem cuttings were used. There were three application rates each for N (0, 80, and 160 kg ha-1), P (0, 40 and 80 kg ha-1), and K (0, 80, and 160 kg ha-1) arranged in a balanced factorial matrix of 27 treatments. After harvest, leaf samples were collected to determine nutrient concentrations and they were split into roots, shoots, leaves, and fruit
 Results: The greatest total dry biomass was produced by the interaction of 80 kg ha-1 N and 40 kg ha-1 P and yielded the following leaf nutrient concentrations (mean ± SD): N 2.9 ± 0.3%, P 0.11 ± 0.01%, K 2.1 ± 0.4%, Ca 1.4 ± 0.7%, Mg 0.34 ± 0.03%, Fe 166.4 ± 49.5 mg kg-1, Cu 6.3 ± 1.7 mg kg-1, Mn 83.3 ± 20.9 mg kg-1, and Zn 22. 6 ± 3.8 mg kg-1. Application of 80 kg ha-1 N and 40 kg ha-1 P could be suggested for commercial fig production.
 Conclusion: Application of 80 kg ha-1 N and 40 kg ha-1 P could be tested under similar commercial production systems; however, the addition of supplemental K deserves further study.

Root rot alters the root-associated microbiome of field pea in commercial crop production systems

Pulses are important crops that have been used to diversify cropping rotations in the Canadian prairies. With increasing cultivation intensity, pulses are becoming more susceptible to root rots. Understanding how root-associated microbiomes are affected by plant health is vital for developing sustainable crop production systems. In this study, we investigated the effect of root rot on rhizosphere and root microbiomes of healthy and root-rot infected field pea (Pisum sativum L.) plants from nine commercial fields in Saskatchewan using amplicon metagenomic sequencing. Bacterial and oomycete α-diversity was higher in diseased root and rhizosphere samples than their healthy counterparts, while fungal diversity was higher in diseased rhizosphere samples. The community structure of root and rhizosphere microbiomes were also affected by the health status of the plants, with root bacterial communities exhibiting the strongest differences. Healthy samples were associated with a higher relative abundance of Rhizobium, Olpidium and Mortierella, and lower abundance of Fusarium and Pythium. Diseased samples had a higher number of indicator genera (42 in diseased vs. 11 in healthy) and more taxa exclusively detected in the diseased core microbiome. Results from this study revealed that root rot generally increased the diversity and altered the community structure and relative abundance of key taxa of the pea root-associated microbiome. This knowledge may aid in the development of microbiome-based disease management strategies, such as identification of key microbial taxa that could help commercial pea growers assess root rot risk.

Seroprevalence of brucellosis in small ruminants and related risk behaviours among humans in different husbandry systems in Mali

Mali has a high pastoral potential with diverse coexisting production systems ranging from traditional (nomadic, transhumant, sedentary) to commercial (fattening and dairy production) production systems. Each of those systems is characterised by close interactions between animals and humans, increasing the potential risk of transmission of zoonotic diseases. The nature of contact network suggests that the risks may vary according to species, production systems and behaviors. However, the study of the link between small ruminants and zoonotic diseases has received limited attention in Mali. The objective of this study was to assess brucellosis seroprevalence and determine how the husbandry systems and human behaviour expose animal and human to infection risk. A cross-sectional study using cluster sampling was conducted in three regions in Mali. Blood was collected from 860 small ruminants. The sera obtained were analysed using both Rose Bengal and cELISA tests. In addition, 119 farmers were interviewed using a structured questionnaire in order to identify the characteristics of farms as well as the risk behaviors of respondents. Husbandry systems were dominated by agro-pastoral systems followed by pastoral systems. The commercial farms (peri-urban and urban) represent a small proportion. Small ruminant individual seroprevalence was 4.1% [2.8-5.6% (95% CI)]. Herd seroprevalence was estimated at 25.2% [17.7-33.9% (95% CI)]. Peri-urban farming system was more affected with seroprevalence of 38.1% [18.1-61.5 (95% CI)], followed by pastoral farming system (24.3% [11.7-41.2 (95% CI)]). Identified risk behaviors of brucellosis transmission to animals were: exchange of reproductive males (30.2%); improper disposal of placentas in the farms (31.1%); and keeping aborted females in the herd (69.7%). For humans, risk factors were: close and prolonged contact with animals (51.2%); consumption of unpasteurized dairy products (26.9%); and assisting female animals during delivery without any protection (40.3%). This study observed a high seroprevalence of brucellosis in small ruminants and also identified risky practices that allow cross transmission between the two populations. This calls for control strategy using a multi-sectoral and multidimensional approach.

Detection of ESBL/AmpC-Producing and Fosfomycin-Resistant Escherichia coli From Different Sources in Poultry Production in Southern Brazil.

This study discussed the use of antimicrobials in the commercial chicken production system and the possible factors influencing the presence of Extended-spectrum β-lactamase (ESBL)/AmpC producers strains in the broiler production chain. The aim of this study was to perform longitudinal monitoring of ESBL-producing and fosfomycin-resistant Escherichia coli from poultry farms in southern Brazil (Paraná and Rio Grande do Sul states) and determine the possible critical points that may be reservoirs for these strains. Samples of poultry litter, cloacal swabs, poultry feed, water, and beetles (Alphitobius sp.) were collected during three distinct samplings. Phenotypic and genotypic tests were performed for characterization of antimicrobial resistant strains. A total of 117 strains were isolated and 78 (66%) were positive for ESBL production. The poultry litter presented ESBL positive strains in all three sampled periods, whereas the cloacal swab presented positive strains only from the second period. The poultry litter represents a significant risk factor mainly at the beginning poultry production (odds ratio 6.43, 95% confidence interval 1–41.21, p < 0.05). All beetles presented ESBL positive strains. The predominant gene was blaCTX–M group 2, which occurred in approximately 55% of the ESBL-producing E. coli. The cit gene was found in approximately 13% of the ESBL-producing E. coli as AmpC type determinants. A total of 19 out of 26 fosfomycin-resistant strains showed the fosA3 gene, all of which produced ESBL. The correlation between fosA3 and blaCTX–M group 1 (blaCTX–M55) genes was significant among ESBL-producing E. coli isolated from Paraná (OR 3.66, 95% CI 1.9–9.68) and these genetic determinants can be transmitted by conjugation to broiler chicken microbiota strains. Our data revealed that poultry litter and beetles were critical points during poultry production and the presence of fosfomycin-resistant strains indicate the possibility of risks associated with the use of this antimicrobial during production. Furthermore, the genetic determinants encoding CTX-M and fosA3 enzymes can be transferred to E. coli strains from broiler chicken microbiota, thereby creating a risk to public health.

Antimicrobial Use in Commercial Poultry Production Systems in Kiambu County, Kenya: A Cross-Sectional Survey on Knowledge, Attitudes and Practices

Background: Antibiotic resistance is putting the commercial poultry production systems across the globe at risk of losses due to the failure of treatments for animals and human health which has been associated with overuse and misuse of antibiotics in poultry farming. One possible approach to the resistance problem is ensuring adequate knowledge, attitudes, and practices on antimicrobial use by farmers on commercial production systems across the globe are applied. Therefore, the current study was carried out to determine factors that could promote antimicrobial use in commercial poultry production systems of Kiambu County, Kenya. Materials and Methods: A descriptive cross-sectional survey was conducted on commercial poultry production systems of Kiambu County to determine the level of knowledge, attitudes, and practices on antimicrobial use. One hundred and fifty-six (n = 156) semi-structured questionnaires imprinted in a mobile Open data kit were administered to capture the level of knowledge, attitudes, and practices on antimicrobial usage in commercial poultry production systems of Kiambu County. Results: Out of the 156 commercial poultry farmers who participated in this study, females accounted for (64%) with 51% of them keeping layers between 50 - 500 birds as medium scale farmers. In the study, most of the farmers were 45 years and above (61%) and they had obtained a secondary level of education (34%). We further noted that 67% of the farmers obtain antibiotics through self-prescription/personal experience. Additional lack of veterinary officers (77%) and consultation services (72%) was also mentioned as key challenges in poultry production. The majority of the respondents (63%), indicated Tetracycline as the most commonly used antibiotic in their farms, with the most preferred subtypes of tetracycline and Amoxicillin being Oxytetracyclines (85%) and Amoxi (88%) respectively. Nearness to veterinary services significantly (P Conclusion: Generally, the level of knowledge and practice on antibiotic use among commercial poultry farmers in Kiambu County is still lacking. This was witnessed with continued use of antibiotics without a prescription from veterinary/livestock extension offices, which is inconsistent with the general recommendation on antimicrobial stewardship. Therefore, the current research calls for intervention measures such as extensive education to farmers on the rational use of antimicrobial agents in commercial poultry disease management, observance of withdrawal periods, and the impacts of residues on public health.