Commercial Milk Research Articles

Construction of surface molecularly imprinted photonic hydrogel sensors with high sensitivity

In comparison with molecularly imprinted photonic hydrogel (MIPH) with specific molecular recognition sites inside matrix, surface molecularly imprinted photonic hydrogel (S-MIPH) is more competitive as a molecular sensor due to presence of molecular recognition sites distributed on surface of hydrogel wall. Nevertheless, the construction of S-MIPH sensors is still challenging owing to the absence of interaction between traditional colloids and imprinted molecules. Here we report the construction of S-MIPH sensors by using poly (styrene-acrylic acid) colloids with abundant carboxyl group that are capable of binding imprinted molecules readily through hydrogen interaction. Benefit from this novel motif, S-MIPH sensors can be readily constructed by self-assembly of poly (styrene-acrylic acid) colloids binding imprinted molecules, the polymerization of precursor solution, as well as finally removal of colloids and imprinted molecules. The self-assembly of poly (styrene-acrylic acid) colloids binding imprinted molecules was investigated in detail. The as-prepared sensor delivers high sensitivity (as low as 10−9 mol L−1), rapid response speed (< 60 s), and good selectivity owing to the 3D macroporous structure and specific molecular recognition sites that are well distributed on surface of hydrogel wall. Additionally, the developed sensors show good sensing performance even in commercial milk, showing great potential for real application.

Black soldier fly as feed ingredient for ruminants.

This paper is a review of some experiments using black soldier fly (BSF) and its by-product to explore their nutritional value, production potential in Indonesia and its application in the ration of ruminants. Evaluation on the effect of milk replacer, creep feed containing BSF, BSF frass and the possibility to use lactic acid bacteria from BSF as probiotics are presented. Utilization of BSF larvae in milk replacer as skim and cream milk substitute showed that there were similarity on physiological, hematological status and performance of goat kids compared to those offered goat milk or commercial milk replacer. In addition, BSF larvae can be used to substitute soybean meal in the creep feed for post weaning goat kids without any differences in weight gain and blood profiles. However, utilization of BSF frass in the fattening goat ration resulted lower digestibility of dry matter and organic matter due to the chitin content in the frass. Black soldier fly larvae grown on chicken manure harbour lactic acid bacteria (LAB) which have potential as probiotics for ruminants. In general, BSF larvae has potential as ingredient for milk replacer, creep feed, fattening ration, and source of LAB for probiotics.

The endocrine and epigenetic impact of persistent cow milk consumption on prostate carcinogenesis

This review analyzes the potential impact of milk-induced signal transduction on the pathogenesis of prostate cancer (PCa). Articles in PubMed until November 2021 reporting on milk intake and PCa were reviewed. Epidemiological studies identified commercial cow milk consumption as a potential risk factor of PCa. The potential impact of cow milk consumption on the pathogenesis of PCa may already begin during fetal and pubertal prostate growth, critical windows with increased vulnerability. Milk is a promotor of growth and anabolism via activating insulin-like growth factor-1 (IGF-1)/phosphatidylinositol-3 kinase (PI3K)/AKT/mechanistic target of rapamycin complex 1 (mTORC1) signaling. Estrogens, major steroid hormone components of commercial milk of persistently pregnant dairy cows, activate IGF-1 and mTORC1. Milk-derived signaling synergizes with common driver mutations of the PI3K/AKT/mTORC1 signaling pathway that intersect with androgen receptor, MFG-E8, MAPK, RUNX2, MDM4, TP53, and WNT signaling, respectively. Potential exogenously induced drivers of PCa are milk-induced elevations of growth hormone, IGF-1, MFG-E8, estrogens, phytanic acid, and aflatoxins, as well as milk exosome-derived oncogenic microRNAs including miR-148a, miR-21, and miR-29b. Commercial cow milk intake, especially the consumption of pasteurized milk, which represents the closest replica of native milk, activates PI3K-AKT-mTORC1 signaling via cow milk’s endocrine and epigenetic modes of action. Vulnerable periods for adverse nutrigenomic impacts on prostate health appear to be the fetal and pubertal growth periods, potentially priming the initiation of PCa. Cow milk-mediated overactivation of PI3K-AKT-mTORC1 signaling synergizes with the most common genetic deviations in PCa, promoting PCa initiation, progression, and early recurrence.

Short-term serum and urinary changes in sex hormones of healthy pre-pubertal children after the consumption of commercially available whole milk powder: a randomized, two-level, controlled-intervention trial in China.

Currently, commercial milk may contain abundant pregnancy-related hormones, the regular consumption of which puts children at a risk of precocious puberty and sex-hormone-associated tumors in adulthood. In this intervention trial, 51 healthy prepubescent children were randomly assigned to the intervention or control arms at a ratio of 3 : 1 to receive 250 or 600 mL m-2 (body surface area) of milk intervention or matching equienergetic sugar water as the control. On testing cow's milk, progesterone was detected, while estrone, estradiol (E2), and testosterone (T2) were not. Cow's milk ingestion did not significantly influence the serum FSH, E2, PRL, LH, and T2 levels (P > 0.05) of pre-pubertal children 3 h after the intervention, while it increased their serum progesterone levels (P < 0.05) when compared with that in the control arm. Regarding the urinary hormone levels, cow's milk ingestion increased the urinary pregnanediol level within 4 h (P < 0.05), but not significantly when compared with that of the control (P > 0.05). The level of pregnanediol and E2 in the morning urine for three consecutive days showed no significant difference between the two arms (P > 0.05). Drinking commercial milk with progesterone influenced the progesterone levels of pre-pubertal children in hours but not days and did not affect other sex hormone levels of pre-pubertal children.

Rework Practices Used During Milk Processing: An Industry Survey

Rework is a common practice in the dairy industry for processors to minimize waste while recovering costs from products that are unsaleable. Regulations related to reworking fluid dairy products are focused on product safety; however, rework in the fluid milk industry and its implications for product quality have not been previously investigated. Our objectives were to characterize current industry practices for reworking fluid dairy products and identify scenarios that could contribute to reduced product quality, particularly microbial spoilage. Seven commercial fluid milk processors from the Pacific Northwest were interviewed regarding their rework handling practices. Processors used various terms (rework, reclaim, and rerun) to describe specific product recovery, storage, and reprocessing procedures. Processors reported nine typical rework motivations, with reclaim and packaging problems the most common; however, rework also played an important role in handling special circumstances. Milk products were reworked as soon as 3 days after production up to the code date (21 days) at dilution rates of ≤20% rework to ≥80% fresh product. Rework conditions with the potential to influence product quality or shelf life of milk products were identified.

THE IMPACT OF DIFFERENT PREPARATION TECHNIQUES FOR CALVING HEIFERS ON THEIR FUTURE DAIRY PRODUCTION

The article presents the results of research on the influence of duration of kneading of udder of springer heifers on further development of mammary gland and future productivity of cows. An important chain of effective dairy farming is the accurate organisation of herd reproduction, preparation of springer heifers for calving and lactation and milking of first calves. It has been determined that during the formation of productivity of dairy animals the first pregnancy has a decisive importance. The specific feature of this physiological state is that complex morphological and functional changes in the reproductive organs happen for the first time in the body, and pregnancy, calving and lactation proceed in the further formation of the animal organism. One of the most significant stressors on the first calving heifer's body is the first birth in combination with machine milking. The inability to overcome the influence of the first milking stress factor is the reason why some first calves are already being eliminated at this phase of the technological chain in commercial milk production. One of the techniques that can reduce the impact of the above mentioned stress factors is udder massage of springer heifers. To study effectiveness of manual massage of udder of springer heifers its duration (from 1 to 4 min.) was tested. There was also studied the effect of different durations of manual massage on the performance of heifers and their morphological traits. The experiment was carried out in SE "Ukrainka Slobodskaya" by IAS NAAS on animals of Ukrainian black-motley dairy breed. For that, five groups were formed with different duration of manual massage (I - 1min, II - 2min, III - 3min, IV - 4min, V - without massage). Massage was started in the 6th month of pregnancy. It was found that kneading of udder of springer heifers compared to no massage increased udder of first calving heifers according to the following measurements: udder girth by 17,4 - 18,7 % (P≤0,05), width 17,9 - 23,7 (P≤0,05), length 15,9 - 20,7 (P≤0,05), depth 16,1 - 20,8 (P≤0,05). It should be noted that using different time of hand massage (groups I-IV) positively shortens the time of litter secretion and increases milk production by 11.7 - 12.3%.

Evaluation of 2'-Fucosyllactose and Bifidobacterium longum Subspecies infantis on Growth, Organ Weights, and Intestinal Development of Piglets.

Human milk is rich in oligosaccharides that influence intestinal development and serve as prebiotics for the infant gut microbiota. Probiotics and 2’-fucosyllactose (2’-FL) added individually to infant formula have been shown to influence infant development, but less is known about the effects of their synbiotic administration. Herein, the impact of formula supplementation with 2’-fucosyllactose (2’-FL) and Bifidobacterium longum subsp. infantis Bi-26 (Bi-26), or 2’-FL + Bi-26 on weight gain, organ weights, and intestinal development in piglets was investigated. Two-day-old piglets (n = 53) were randomized in a 2 × 2 design to be fed a commercial milk replacer ad libitum without (CON) or with 1.0 g/L 2’-FL. Piglets in each diet were further randomized to receive either glycerol stock alone or Bi-26 (109 CFU) orally once daily. Body weights and food intake were monitored from postnatal day (PND) 2 to 33/34. On PND 34/35, animals were euthanized and intestine, liver and brain weights were assessed. Intestinal samples were collected for morphological analyses and measurement of disaccharidase activity. Dry matter of cecum and colon contents and Bifidobacterium longum subsp. infantis abundance by RT-PCR were also measured. All diets were well tolerated, and formula intake did not differ among the treatment groups. Daily body weights were affected by 2’-FL, Bi-26, and day, but no interaction was observed. There was a trend (p = 0.075) for greater total body weight gain in CON versus all other groups. Jejunal and ascending colon histomorphology were unaffected by treatment; however, there were main effects of 2’-FL to increase (p = 0.040) and Bi-26 to decrease (p = 0.001) ileal crypt depth. The addition of 2’-FL and/or Bi-26 to milk replacer supported piglet growth with no detrimental effects on body and organ weights, or intestinal structure and function.

Total plate count of commercial pasteurized milk sold by street vendors in Mulyorejo sub-district Surabaya

Food and nutritional quality of society is one of mine focuses of the government. Milk have complete nutrients but also can be the source food borne disease. The Veterinary Public Health itself has the responsibility to provide guarantees of food safety from animal resources which is called ASUH (safe, healthy, wholesome and halal) to be consumed by society. This study aimed to know the Total Plate Count of commercial pasteurized milk sold by street vendors in Mulyorejo Sub-district Surabaya. The study was conducted from January until February 2018 at the Laboratory of Veterinary Public Health Department, Faculty of Veterinary Medicine, Universitas Airlangga. Thirty samples of commercial pasteurized milk were bought from three street vendors then examined using Pour Plate Method of Total Plate Count. Total Plate Count result showed that the number of bacteria in commercial pasteurized milk sold by street vendors in Mulyorejo Sub-district Surabaya was 1.0 x 104 CFU/ml, that met the National Standard of Indonesia (SNI) 01-3951-1995.

Rumen Development of Artificially-Reared Lambs Exposed to Three Different Rearing Regimens.

Simple SummaryYoung ruminants possess an undeveloped rumen when first born. Encouraging good rumen development in early life is vital for young ruminants as they transition to a solid feed diet, to ensure optimum growth post-weaning. This study aimed to investigate the effect of three different rearing regimens on rumen development in lambs reared artificially. Weaning of lambs at 42 d of age improved rumen fluid n-butyric content and also resulted in a thicker rumen dorsal wall. Feeding lambs pellets with low fibre and weaning them early increased rumen fluid n-valeric content. Papillae width, density, and rumen wall muscle thickness were affected by rearing treatment. Empty rumen weight, rumen volume, papillae development, and rumen fluid iso-butyric and iso-valeric content had positive associations with dry matter intake and nutrient intakes from solid feed. The majority of volatile fatty acids in the rumen fluid had a positive association with papillae height on the rumen dorsal wall. These results suggest that lamb diet and age at weaning influenced rumen function and physical development. Further studies examining how rumen microbial composition and rumen gene expression are influenced by rearing regimens are required.The objective of this study was to examine the effect of three different rearing regimens on rumen development in lambs reared artificially. Romney ram lambs were randomly allocated to one of three treatments: commercial milk replacer fed to 57 d of age and high fibre concentrate pellets (HFP57); commercial milk replacer, high fibre concentrate pellets, and early weaning from milk replacer at 42 d of age (HFP42); high protein milk replacer from 2–16 d of age followed by commercial milk replacer, low fibre concentrate pellets, and early weaning from milk replacer at 42 d of age (LFP42). Lambs were slaughtered at 57 d of age. Volatile fatty acid content in rumen fluid at slaughter was analysed and rumen tissue samples were collected for histological examination. The rumen n-butyric content was greater (p < 0.05) in both LFP42 and HFP42 treatment lambs compared to HFP57 lambs. The n-valeric content was greater (p < 0.05) in LFP42 lambs compared to both HFP57 and HFP42 treatment lambs. Thickness of the rumen dorsal wall determined by ultrasound scanning at 49 d was greater (p < 0.05) in both HFP42 and LFP42 lambs compared to HFP57 lambs. There was an interaction (p < 0.05) between treatment and site of rumen tissue sampling on papillae width, density, and rumen muscular layer thickness. Collectively, early weaning and the provision of a low fibre pellet leads to improved rumen function and physical development.

Paths for improvements of smallholder dairies: Case-study on local food security in arid regions of China

The trend today leans towards urbanization and large farming enterprises. However, in 2019–2020, with the impact of the COVID-19 pandemic, the supply of commercial milk was disrupted, and the local smallholder dairy farmers played an important role in stabilizing food and nutritional security in rural, arid areas of China. The aim of this study was to describe and understand the operation of smallholder dairy farms and suggest what improvements are needed. We interviewed 45 smallholder dairy farmers from Gansu Province. A questionnaire was used to collect information on the dairy operation, including, number of cows, cow breeds, feed offered, milk yield and on management and open-ended discussions on the needs of the farmers to improve their dairies. Feed samples were collected at each farm to determine composition and milk samples were collected to analyze for composition and fatty acid content, and to compare the quality with commercial milk. Smallholder farmers generally fed dairy cows local, low-quality forages, mainly wheat straw and corn stover (CS/WS), and the milk yield was low, however, milk had a high content of beneficial unsaturated and polyunsaturated fatty acids. Milk from smallholder farms had lower concentrations of undesirable saturated fatty acids, higher concentrations of beneficial unsaturated and polyunsaturated fatty acids, and a lower atherogenic index than commercial milk. Proper sanitation measures and veterinary care were often lacking in these remote areas, leading to high somatic cell counts (SCC). A modified version of grounded theory was used to construct categories of what the farmers perceived was needed to improve their dairies. Four categories emerged in the following order: 1) equipment and management, 2) government support, 3) better cows, and 4) better feed. In a follow-up survey in 2020 of 18 farmers, conditions generally remained the same, including feed offered the animals, which would indicate that the milk composition remained the same. It was concluded that smallholder dairy farmers can provide food and nutritional security, especially for local residents in remote, rural, arid areas, but improvements in facilities, management, feed, cow breeds and sanitation are needed. Government subsidies should be implemented to help the smallholder dairy farmers. Results from this study provide information for local governments in their decision-making policies to stabilize smallholder dairies.

Presence of Aflatoxin M1 in Commercial Milk in Paraguay

The presence of aflatoxin M1 (AFM1) in milk is a public health concern because milk is a significant part of human diets worldwide. In economies where AFM1 monitoring is low or nonexistent, the possibility of AFM1 contamination might be increased. Our study was conducted to detect and quantify AFM1 in fluid milk and milk drinks of various brands, fat concentrations, packages, and heat treatments sold in the Metropolitan Area of Asunción, Paraguay. Eighty samples were collected from supermarkets in the Metropolitan Area of Asunción following a nonprobability sampling method. An enzyme-linked immunosorbent assay for AFM1 (25 to 500 ppt) was used to quantify the toxin, and results were analyzed with nonparametric methods. All samples were positive values for AFM1 (above the detection limit of 25 ng/kg); 85% of the samples had 30 to 50 ng/kg, and 15% had >500 ng/kg. No significant difference in AFM1 concentration was found based on fat concentration, heat treatment, or type of packaging of these milk products; however, significant differences were found between brands.

Dairy productivity of Kazakh horse mares

The work aimed to establish the influence of udder parameters of Kazakh mares on their formation of dairy productivity and the growth rate of foals during the milk period. The cup-shaped udder is characterized by a large base (the udder circumference at the base is 70.3 cm), the symmetrical arrangement of both udder lobes, with well-developed cylindrical nipples, the nipples are widely spaced between the two lobes. The round shaped udder is instead characterized by conical and short nipples and a smaller udder volume. For four months of lactation, 1051 liters of commercial milk were produced from mares with cup-shaped udder, while 823.6 liters- with round-shaped udder.The observation results showed that foals from mares with the cup-shaped and round - shapedudders on the first day of life were 39.8 and 39.6 kg of live weight, respectively, then after a month, it doubles, reaching 82.5 and 79.5 kg. The highest indicators of absolute gain - 42.7 kg, average daily gain - 1.42 kg, and relative gain -107.3% were observed in offspring with a cup-shaped udder. In foals delivered from mares with a round-shaped udder, the gain rates (39.9; 1.33 kg and 100.7%, respectively) were slightly lower.

Examining the Environmental Impacts of the Dairy and Baby Food Industries: Are First-Food Systems a Crucial Missing Part of the Healthy and Sustainable Food Systems Agenda Now Underway?

Food systems are increasingly being understood as driving various health and ecological crises and their transformation is recognised as a key opportunity for planetary health. First-food systems represent an underexplored aspect of this transformation. Despite breastfeeding representing the optimal source of infant nutrition, use of commercial milk formula (CMF) is high and growing rapidly. In this review, we examine the impact of CMF use on planetary health, considering in particular its effects on climate change, water use and pollution and the consequences of these effects for human health. Milk is the main ingredient in the production of CMF, making the role of the dairy sector a key area of attention. We find that CMF use has twice the carbon footprint of breastfeeding, while 1 kg of CMF has a blue water footprint of 699 L; CMF has a significant and harmful environmental impact. Facilitation and protection of breastfeeding represents a key part of developing sustainable first-food systems and has huge potential benefits for maternal and child health.

Growth and Body Composition of Artificially-Reared Lambs Exposed to Three Different Rearing Regimens.

Simple SummaryOptimum lamb growth and cost-effective artificial lamb rearing are important to maximise farm profit. In addition to milk feeding, supplementation of a solid feed with balanced nutrients and an adequate level of fibre will aid rumen development, allowing for a smooth transition from a liquid to a solely solid diet. This study aimed to determine the effect of pellet fibre level, milk replacer composition and early milk weaning at 42 days of age, on the growth and body composition of lambs reared artificially to 57 days of age. Results demonstrate that lamb growth rate to 57 days of age was not affected by early weaning or pellet fibre level. Early milk weaning increased pellet intake and empty rumen weight. Early weaning resulted in leaner carcasses due to reduced fat deposition and lower total feed costs compared to lambs offered milk to 57 days of age. Overall, feeding lambs either low or high fibre pellets and weaning early (42 days of age) was shown to be beneficial, as growth was similar to unweaned lambs, but leaner carcasses and reduced feed costs were observed.This study was designed to investigate the influence of pellet fibre level, milk replacer composition and age at weaning on growth and body composition of lambs reared artificially. Romney ram lambs were randomly allocated to one of three rearing treatments; HFP57: commercial milk replacer to 57 days of age, and high fibre concentrate pellets; HFP42: commercial milk replacer with early weaning at 42 days of age, and high fibre concentrate pellets; LFP42: high protein milk replacer from 2–16 days of age followed by commercial milk replacer with early weaning at 42 days of age, and low fibre concentrate pellets. Lambs were slaughtered at 57 days of age. Overall average daily liveweight gain of lambs did not differ (p > 0.05) between treatments. Dressing out percentage, carcass weight, empty small intestine and omental fat were higher (p < 0.05) in HFP57 than in both HFP42 and LFP42 lambs. HFP42 and LFP42 lambs had heavier (p < 0.05) empty rumen weights. Whole body protein content was higher (p < 0.05) in HFP42 lambs compared to both HFP57 and LFP42 lambs. Fat content and daily fat deposition were greater (p < 0.05) in HFP57 lambs than HFP42 and LFP42 lambs. Weaning lambs at 42 days of age with provision of either low or high fibre concentrate pellets, resulted in similar growth rates, reduced whole body fat deposition and was a more cost-effective rearing regimen.

Beliefs and Norms Associated with the Use of Ultra-Processed Commercial Milk Formulas for Pregnant Women in Vietnam.

Commercial milk formula for pregnant women (CMF-PW) is an expensive, ultra-processed food with a high concentration of sugar, the consumption of which may be linked to negative health outcomes. However, CMF-PWs are promoted as beneficial for pregnant women and lactating mothers as well as their children. To date, little is known about the factors associated with the use of CMF-PW among pregnant women. We performed this analysis to examine the association between the use of CMF-PW and related beliefs and norms among pregnant women in Vietnam. We interviewed 268 pregnant women in their second and third trimesters from two provinces and one municipality representing diverse communities in Vietnam. Multinomial (polytomous) logistic regression, structural equation modeling (SEM), and propensity score matching (PSM) analysis were used to examine associations between beliefs and social norms related to CMF-PW and reported consumption, characterized as occasional, recent, and never during the current pregnancy. Overall, 64.6% of pregnant women reported using CMF-PW during the current pregnancy and 34.7% consumed CMF-PW on the day prior to the interview. Strong beliefs that CMF-PW will make a child smart and healthy (53.7%) and the perception that use of CMF-PW is common (70.9%) were associated with increased use on the previous day (beliefs: aOR: 3.56; 95% Confidence Interval (95% CI): 1.65, 7.71; p < 0.01 and social norms aOR: 2.29; 95% CI: 1.13, 4.66; p < 0.05). SEM and PSM analyses confirmed these findings for both occasional and regular CMF-PW use. Results are consistent with observations of CMF-PW product labels and marketing tactics in Vietnam. The prevalent use of CMF-PW in Vietnam is associated with the belief that these products make children smart and healthy and the perceived social norm that most mothers use these products, which mirrors marketing messages and approaches employed by the CMF industry.

Effect of TBC of raw milk and thermal treatment intensity on endotoxin contents of milk products

Endotoxin in dairy products may pose risks to consumers’ health, but the effects of raw-milk total bacterial count (TBC) and thermal treatment temperature on endotoxin content of dairy products are not clear. In this study, TBC of raw milk and processed milk with different thermal treatment temperatures were detected by pilot processing experiment. We then verified it by detecting the endotoxin content of commercial dairy products. TBC was determined by conventional culture method, and the changes in alkaline phosphatase (ALP) and lactoperoxidase (LPO) activities in bovine milk before and after heat treatment were determined. The levels of fatty acids in bovine milk were detected by gas chromatography using a flame ionization detector (GC-FID). In the pilot experiment, raw milk was treated at 72, 75, 80, 85, 90, 95, 100, 105, 110, 115, and 120 °C for 15 s. After that, the raw milk was stored at 20 °C for 24 h and 48 h to greatly increase the TBC, and treated at 75 °C for 15 s and 90 °C for 15 s. The results show that the endotoxin content of sterilized milk increased with the increase in TBC and thermal treatment temperature. With the increase in thermal treatment temperature, the activities of ALP and LPO with endotoxin inhibition decreased. In addition, a total of 226 dairy samples were tested. The endotoxin content of 37 commercial PM milk samples were 6 EU/ml to 231 EU/ml, with 31 EU/ml to 1437 EU/ml for 40 commercial UHT milk samples, and 6 EU/ml to 9080 EU/ml for 149 reconstituted IFM samples. There was a significant difference in the endotoxin content of different dairy products (P < 0.05), and there was no significant difference between domestic and imported IFM (P > 0.05). This study found that TBC and thermal treatment temperature of raw milk could affect the endotoxin content of dairy products.

Antibacterial characterization of erythorbyl laurate against Geobacillus stearothermophilus spores

We evaluated the antibacterial activity of erythorbyl laurate (EL) against Geobacillus stearothermophilus and its spore in aqueous medium and in oil-in-water (O/W) emulsion. The effective concentrations of EL required to kill vegetative cells and inhibit spore germination were determined. EL exerted bactericidal effects on the vegetative cells at concentrations of 200 and 1500 ppm in the aqueous and O/W emulsion systems, respectively. In addition, EL inhibited spore germination at 200 and 2000 ppm in the aqueous and O/W emulsion systems, respectively, and treatment with concentrations exceeding 7500 ppm killed spores in the emulsion. The germination inhibition activity of EL was verified by determining the dipicolinic acid release from the spores. The antibacterial activity of EL in canned milk coffee indicated that the viable number of spores decreased with increasing EL concentration. Furthermore, the sporicidal activity of EL was greatly enhanced when the spores were exposed to heat treatment at 121 °C in the commercial milk coffee environment, which implies that EL can be used to control contamination of emulsion-based foods.

Explore Gastric Lipolysis and Lipid Oxidation of Conventional versus Pasture-Based Milk by a Semi-dynamic In Vitro Digestion Model.

Research on gastric lipolysis of commercial cow's milk with different fatty acid (FA) compositions is scarce. Gastric lipase exhibits specificity for the sn-3 chain position of triacylglycerols, whose structure is influenced by milk FA composition. Therefore, during gastric digestion of conventional (C) vs pasture-based (P) milk, differences may occur on lipolysis, which has impact on free FA available, influencing their absorption/metabolism rate and physiological hormonal responses. Those two milk types were subjected to the INFOGEST semi-dynamic digestion model. Five gastric emptying points were analyzed for oxidative degradation of polyunsaturated fatty acids (PUFA) and individual free FA. The relative release of medium-chain FA (C8:0-C12:0) was higher than that of longer-chain FA (C14:0-C18:0), and a linear increase in markers of PUFA oxidative degradation occurred along gastric digestion. Quantitatively, C8:0, C18:2n-6, C18:3n-3, and CLAc9t11 were higher (P < 0.001) in P milk when compared with C milk.

Discovery of Natural Veterinary Herbal Medicine Products in Commercial Cow Milk by Using Nontargeted Profiling on LC–ESI–TOF Platform

Discovery of Natural Veterinary Herbal Medicine Products in Commercial Cow Milk by Using Nontargeted Profiling on LC–ESI–TOF Platform

Stability of bioactive compounds in liposomes after pasteurisation and storage of functional chocolate milk

SummaryIn the present study, liposomes with bioactive compounds (BC), vitamin E (VE) and vitamin C (VC), were characterised after pasteurisation and storage in chocolate milk. Liposomes were obtained using soy phosphatidylcholine (SPC), SPC with stearic acid (SA) or calcium stearate (CaS). Oxidative stability, size, shape and membrane packing were studied after pasteurisation and storage for 28 days at 4 °C. Oxidative stability was also studied after pasteurisation in extreme conditions: 6 months, 25 °C and light. The oxidative level determined in liposomes is much under the permitted limits, generating good quality food. SPC maintained stability in size, shape and membrane packing for 28 days at 4 °C. SPC:SA and SPC:CaS evidenced a decrease in liposomal aggregation and a higher membrane packing for 28 days at 4 °C, which favoured stability. These results were optimum because commercial functional milk must have a shelf life of 7 days at 4 °C. Pasteurised liposomes were incorporated in chocolate milk to study the sensory (after storage for 7 days at 4 °C) and microbiological stability. None of the liposomes contributed to the microbial load. SPC:SA presented high sensorial stability in the milk, being a potentially suitable medium to incorporate BC generating functional food.