To convert biomass into biofuel, pretreatment is the first stage required to de-structure lignocellulose – twin-screw extrusion is one of the viable pretreatment technologies. The enzymatic hydrolysis of corncobs pretreated with twin-screw extrusion to obtain sugar was evaluated. Corncob extrusion (115–130 °C; 14 rpm) was enhanced through the employment of additives (water and glycerol, 25:25, % w/w). By reproducing the response surface methodology (RSM) technique, the maximized glucose productivity (0.69 g L −1 h −1 ) and conversion of cellulose to glucose (90.4 % w/w), as well as hemicellulose to xylose and arabinose (44.0 % w/w) were established with the dosage of the commercial enzymatic complex Cellic Ctec2 (32 FPU/g dry lignocellulosic material ) and solids loading (17.8 % w/w). Total sugar yield was of 471 kg (glucose 323 kg; xylose and arabinose 148 kg) for a dried corncob ton. Kinetic constants of the Michaelis-Menten model, V max and K m , for converting cellulose to glucose were of 6.00 % (w/w)/h and 22.59 g cellulose /L solution , respectively. A residue-free and effective corncob extrusion pretreatment enhanced high solids loading enzymatic hydrolysis to achieve a glucose-rich solution. • Corncob clean extrusion pretreatment favored enzymatic hydrolysis for sugar release. • Corncob extrusion was optimized using additives (water and glycerol, 25:25, % w/w). • Glycerol as a corncob extrusion additive did not interfere in the enzymatic hydrolysis • Higher extruded corncob loading favored high cellulose-to-glucose conversion (> 90 %). • Kinetic study showed 40.6 %-time improvement for extruded corncob enzymatic hydrolysis.