BackgroundAdolescents undergo a period of motor incoordination during puberty characterized by high movement variability. It is unknown if differences in running kinematics variability exist among adolescent long-distance runners. Research questionIs kinematic variability different among male and female adolescent long-distance runners of different stages of physical maturation? MethodsWe enrolled 114 adolescent long-distance runners (ages 8–19, F = 55, M = 59) in this secondary analysis of a larger cross-sectional study. Participants completed a three-dimensional overground running analysis at a comfortable self-selected speed. Peak frontal, sagittal, and transverse plane hip, knee, and ankle/shoe joint angles from the right leg were identified during stance phase for at least five trials. Variability in running kinematics was quantified as the standard deviation of the peak joint angles among the running trials for each participant. Participants were stratified by sex and stage of physical maturation (pre-, mid-, post-pubertal) and two-way ANOVAs compared between-subjects variability among groups (p ≤ .05). ResultsSignificant sex by maturation interactions were observed for hip external rotation and ankle external rotation variability. Sex differences were observed for hip internal rotation, with males demonstrating greater variability, and ankle internal rotation, with females demonstrating greater variability. Pre-pubertal runners demonstrated significantly greater variability than mid-pubertal runners for hip flexion, and greater variability than post-pubertal runners for hip flexion, hip adduction, hip internal rotation, and knee flexion. SignificancePre-pubertal adolescent long-distance runners demonstrate greater stance phase variability in running kinematics than post-pubertal adolescent long-distance runners, while adolescent males and females demonstrate similar variability. Anthropometric and neuromuscular changes that occur during puberty likely influence running patterns and may contribute to more consistent kinematic patterns for post-pubertal runners.