Combined Detection Of Carbohydrate Antigen 125 Research Articles

Value of TCT combined with HPV and CA125 in early cervical cancer screening in a medical examination population.

This research aimed to explore the clinical value of thin prep cytologic test (TCT) combined with human papillomavirus (HPV) and carbohydrate antigen 125 (CA125) in early cervical cancer screening in the physical examination population. For this purpose, a total of 3587 females who received gynecological physical examination in the outpatient department of Ganzhou people's Hospital from January 2018 to March 2022 were included and underwent TCT, HPV and carbohydrate antigen 125 upon admission. Colposcopy biopsy was performed on patients who tested positive for any of the three indicators. Then using pathological diagnosis as the gold standard, the three methods applied alone or in combination were evaluated in terms of sensitivity, specificity, diagnostic yield and Youden index. Results showed that Among the 3587 females, 476 (13.27%) were HPV positive, 364 (10.14%) CA125 positive, and 314 (8.75%) TCT positive. Furthermore, 738 tested positive for any of the three indicators and underwent cervical biopsy. Among the 738 cases, 280 (39.94%) developed chronic cervicitis, 268 (36.31%) low-level cervical intraepithelial neoplasia (CIN), 173 (23.44%) high-level CIN, and 17 (2.30%) cervical cancer. HPV+TCT+CA125 combined screening showed higher sensitivity (94.54%), specificity (83.92%), diagnostic coincidence rate (87.46%) and Youden index (0.760) than single-indicator examinations. Also, it had the largest area under the receiver operating characteristic (ROC) curve, 0.673 (0.647, 0.699), compared to any other screening method. In conclusion, The combined detection of CA125, HPV and TCT is of clinical significance due to its higher sensitivity and accuracy in early screening of cervical cancer in the physical examination population.

Diagnostic value of serum tumor biomarkers for endometrial cancer in elderly women with intrauterine abnormalities on transvaginal ultrasound

Objective To investigate age-related changes in serum tumor markers carbohydrate antigen 125(CA125), CA153 and CA199, and the clinical significance of separate and combined detection of these markers for screening endometrial carcinoma(EC)in elderly women with intrauterine abnormalities on transvaginal ultrasound(TVS). Methods Cross-sectional data of 420 elderly women suspected of having an intrauterine abnormality by TVS and undergone hysteroscopy with dilation and curettage from January 2010 to December 2017 were retrospectively analyzed.Patients were divided into a 60-64 years-old group and a 65-83 years-old group.Aging-related changes in positive rates of CA125, CA153 and CA199 were compared between the two groups.Differences in positive rates of tumor markers were compared between different pathological types.Using postoperative pathological diagnosis as the gold standard for EC, the diagnostic sensitivity, specificity and accuracy of serum CA125, CA153 and CA199 alone or in combination for EC were calculated. Results The positive rates of CA125, CA153 and CA199 were 3.9%(16/412), 0%(0/172)and 5.0%(20/404), respectively.The positive rate of CA125 was higher in the 65-83 years-old group(6.5%, 16/246)than in the 60-64 years-old group(0.0%, 0/166)(P=0.001). The positive rates of CA125 and CA199 in patients with EC were 14.3%(4/28)and 42.9%(12/28), which were higher than those in patients with other pathological types.However, the sensitivity of single and combined detection of CA125 and CA199 was too low for the diagnosis of EC(14%、43%). Conclusions The single and combined detection of CA125, CA153 and CA199 for screening EC is of limited value in elderly women.New tumor markers need to be identified and used in combination with TVS for screening EC in elderly women. Key words: Endometrial neoplasms; Transvaginal sonography; Tumor biomarker

Diagnostic value of combined detection of CA125, HE-4 and D-Dimer in ovarian cancer

Objective To investigate the separate and combined detection value of serum carbohydrate antigen 125 (CA125), human epididymis protein 4 (HE-4) and D dimer (D-dimer) in the diagnosis of ovarian cancer. Methods One hundred and twenty ovarian cancer patients were selected as the observation group, one hundred and twenty patients with ovarian benign tumor as the benign group, eighty women with healthy physical examination results as the control group, then the chemiluminescence immunoassay was used to detect the expression of serum CA125 and HE-4 of the three groups, and the immune turbidimetric method was applied to examine the expression of D-dimer.At last, the diagnosis efficiency of CA125, HE-4 and D-dimer in separate and combined detection was calculated. Results CA125 in the observation group was ( 623.07±274.18) U/ml, HE-4 was (594.22±329.068) ng/ml and D-dimer was (418.57±276.75) ng/L, CA125 in the benign group was (45.09±32.58) U/ml, HE- 4 was (97.92±57.52) ng/ml and D-dimer was (204.52±80.07) ng/L; CA125 in the control group was (40.23±28.16) U/ ml, HE-4 was (85.65±37.27) ng/ml and D-dimer was (187.57±65.74) ng/L, the differences between the three groups were statistically significant (F=122.82, 89.91, 54.46; P<0.05). The level of CA125 in the serum of patients with advanced stage (stage III-IV) was (586.10±278.33) U/ml, HE-4 was (437.49±238.06) ng/ml, D-dimer was (493.78±274.45) ng/L, in the early stage (stage I-II), the level of CA125 in the serum of patients with ovarian cancer was (372.12±265.31) U/ml, HE-4 was (673.64±301.68) ng/ml, D-dimer was (364.84±267.54) ng/L, and the difference was statistically significant ( t=4.244, 4.78, 2.560, P<0.05). The detection sensitivity and specificity of CA-125 were 71.67% and 62.50% respectively.The sensitivity of HE-4 was 74.17% and the specificity was 75.00%.The sensitivity of D-dimer was 62.5% and the specificity was 60.00%.The combined detection sensitivity of the three was 80.00% and the specificity was 77.5%. Conclusion The sensitivity and specificity of the three tumor markers in combined detection were higher than those of separate detections, so the combined detection of CA125, HE-4 and D-dimer can further improve the diagnosis level of ovarian cancer. Key words: Carbohydrate antigen 125; Human epididymal protein 4; D-dimer; Ovarian cancer

Expression of epidermal growth factor receptor, carbohydrateantigen 125, and carbohydrateantigen 199 in postoperative non-small cell lung cancer patients with bone metastasis

Objective To investigate the expression of epidermal growth factor receptor (EGFR) in the tissue of non-small cell lung cancer (NSCLC), and the expression of carbohydrateantigen 125 (CA125), and carbohydrateantigen 199 (CA199) in postoperative NSCLC patients with bone metastasis and without bone metastasis, and discuss the roles of EGFR, CA125 and CA199 in postoperative NSCLC patients with bone metastasis. Methods Sixteen cases of postoperative NSCLC with bone metastasis served as the experimental group, and 16 cases of postoperative NSCLC without bone metastasis as the control group. The expression of EGFR in the lung cancer, and the serum levels of CA125 and CA199 were determined. Results The strong positive rate of EGFR was 81.2% in the experimental group. The CA125 and CA199 positive rate in the experimental group was 56.3% and 43.8% respectively. Combined detection positive rate was 81.3%. The strong positive rate of EGFR was 6.2% in the control group. The CA125 and CA199 positive rate in the control group was 0. Combined detection positive rate was 0. The serum levels of CA125 and CA199 in the experimental group were (138.05±141.06) Ku/L and (78.06±112.86) U/ml, and those in the control group were (9.94±6.50) Ku/L and (9.40±4.66) U/ml respectively. Conclusion With the high expression of EGFR, combined detection of CA125 and CA199 has certain significance in the early diagnosis of bone metastasis in postoperative NSCLC patients. Key words: Non-small cell lung cancer; Bone metastasis; Epidermal growth factor receptor; Carbohydrateantigen 125; Carbohydrateantigen 199