Combination Of Cryotherapy Research Articles

The feasibility of the deep hypothermal cryotherapy plus immune checkpoint inhibitor for treatment of severe malignant central airway obstruction.

The recurrence shortly after interventional therapy poses a great challenge in managing malignant central airway obstruction (MCAO). While cryotherapy has shown potential benefits when combined with immunotherapy in lung cancer, its effectiveness for improving local control of malignant central airway tumors is not well understood. This study aims to evaluate the clinical efficacy and safety of combining these strategies. A total of 12 patients with MCAO who had been treated with a combination of cryotherapy and immune checkpoint inhibitors (ICIs) were compiled for analysis in a tertiary hospital in Shanghai East Hospital, China. The primary endpoint is the duration of efficacy (DoE) for MACO after the combined therapy, which was defined as the time interval of the airway stayed patency without additional repeated interventional treatment. Secondary endpoints encompassed an assessment of objective response rate, overall survival and the adverse events. Twelve patients received a combination therapy between January 2021 and December 2023. Median age was 66 years, 91.7% male. Fifty percent were squamous carcinoma. 50.0% had medium to low programmed cell death-ligand 1 (PD-L1) levels. 41.7% patients' performance status scored higher than 2. 91.7% (11/12) of the patients objectively respond to the combined therapy, with a median DoE of 10.0 (95% confidence interval: 3.7-21.7) months. The 6-, 12- and 18-month survival rate was 75.0%, 50.0% and 41.8%, respectively. The most common adverse event was granulation. Other events included mild rash, pneumonitis, and hypothyroidism. No severe procedure-related adverse events occurred. In patients with endoluminal or mixed MCAO, our findings suggest that combining endobronchial cryotherapy with ICIs may help maintain airway patency, offering a promising preliminary signal for this therapeutic approach.

Experience in the use of cryotherapy in patients after total replacement

BACKGROUND: All over the world, total joint replacement is one of the most popular surgical technologies due to the widespread prevalence of joint diseases, as well as injuries. There is no doubt that rehabilitation is a necessary component of the complex treatment of the patient and plays an important role in the postoperative period. Physiotherapeutic methods of treatment occupy a special place in rehabilitation programs. Cryotherapy has been used for many years in the pathology of the musculoskeletal system and has proven its clinical effectiveness due to analgesic, anti-inflammatory and decongestant effects, which gives grounds for its use after surgical interventions.
 AIM: Based on the above, the purpose of this study was to study the effectiveness and rationale for the use of cryotherapy in combination with therapeutic gymnastics procedures in patients after total knee replacement for the third the rehabilitation stage.
 MATERIALS AND METHODS: The study included 60 patients after total knee replacement at the age of 57 to 79 years. All patients were admitted for outpatient treatment 3 months after surgery and were divided into 2 groups. The first group consisted of 30 patients who had therapeutic gymnastics classes with a physical therapy instructor (physical therapy), for a course of 10 daily procedures. The second group consisted of 30 patients who underwent a comprehensive rehabilitation program, including therapeutic gymnastics and air cryotherapy, for a course of 10 daily procedures.
 RESULTS: In all patients, before the start of the rehabilitation course, the intensity of the pain syndrome according to VAS averaged 4.5±0.6 points; according to the 10-meter walking test, a decrease in the level of mobility was detected, in most patients, the sum of the points on the two parts of the KSS scale averaged 62.8±4.5; according to the results of laser Doppler flowmetry, 44% of the subjects revealed spastic, and in 56% ― spastic-stagnant type of microcirculation. After a course of complex rehabilitation treatment, a significantly more significant reduction in pain syndrome (according to VAS) and microcirculatory disorders (according to laser Doppler flowmetry), improvement in the function of the operated joint (according to the KSS scale) was revealed, compared with the use of physical therapy as a monomethod, which contributed to an increase in the level of mobility of patients.
 CONCLUSION: The combined use of air cryotherapy and therapeutic gymnastics in patients after total knee replacement contributes to the formation of pronounced analgesic, anti-inflammatory and vasocorrecting effects, which allowed to increase the effectiveness of treatment at the 3rd stage of rehabilitation treatment.

The Influence of Interval Training Combined with Occlusion and Cooling on Selected Indicators of Blood, Muscle Metabolism and Oxidative Stress.

The study included 30 young, healthy and untrained people. The VASPER (Vascular Performance) training system was used-High-Intensity Interval Training with the simultaneous use of occlusion and local cryotherapy. Blood from the project participants was collected six times (2 weeks before the start of training, on the day of training, after the first training, after the 10th training, after the 20th training and two weeks after the end of training). The subjects were randomly divided into three groups: exercises only (controlled), with occlusion and with occlusion and local cryotherapy. Statistical analysis of changes in the average values of indicators in all study groups showed a significant change increase due to the time of testing IGF-1 (F = 2.37, p = 0.04), XOD (F = 14.26, p = 0.00), D-Dimer (F = 2.90, p = 0.02), and decrease in MDA (F = 7.14, p = 0.00), T-AOC (F = 11.17, p = 0.00), PT Quick (F = 26.37, p = 0.00), INR (F = 8.79, p = 0.00), TT (F = 3.81, p = 0.00). The most pronounced changes were observed in the occlusion and cooling group. Both interval training without and with the modifications used in the study influences coagulation and oxidative stress parameters and, to a small extent, muscle metabolism. It seems reasonable to use occlusion and local cryotherapy in combination with occlusion.

Cryotherapy and immune checkpoint inhibitor therapy for the treatment of metastatic non-small cell lung cancer

Lung cancer remains among the most frequently diagnosed and deadliest diseases in the world. While immune checkpoint inhibitors revolutionized the cancer treatment landscape, only a relatively small proportion of patients benefit from this treatment, likely due to the ability of cancer cells to evade or even exclude the immune system from tumors. Cryotherapy offers a possible way to counteract this process by inducing an inflammatory response and helping in cancer neoantigen presentation, increasing the effectiveness of immunotherapy. While preclinical studies seem to support this claim, further investigation is needed to conclude the benefits and risks of cryotherapy in combination with immune checkpoint inhibitors.

Efficacy and safety of microneedling, fractional CO2 laser, and cryotherapy combined with 5-aminolevulinic acid photodynamic therapy in the treatment of actinic keratosis: A multicenter prospective randomized controlled study

BackgroundPhotodynamic therapy (PDT) for actinic keratosis (AK) is limited by the depth of treatment. Microneedling or fractional CO2 laser can facilitate the penetration of photosensitizer, while cryotherapy can treat deeper tissues but is not suitable for field cancerization. ObjectiveTo investigate the efficacy of microneedling, fractional CO2 laser, and cryotherapy in combination with PDT for AK. MethodsPatients with AK were randomized into 4 groups, including group A with microneedling + PDT, group B with fractional CO2 laser + PDT, group C with cryotherapy + PDT, and group D with PDT. After 12 weeks, the clinical, dermoscopic, and reflectance confocal microscopy (RCM) outcomes were assessed. ResultsA total of 129 patients were included in this study, with 31, 30, 35, and 31 patients in each group, yielding clinical response rates of 90.3%, 93.3%, 97.1%, and 74.2%, respectively (P=0.026). The RCM response rates were 71.0%, 80.0%, 85.7%, and 54.8%, respectively (P=0.030). The dermoscopic response rates were 77.4%, 83.3%, 88.6%, and 60.0%, respectively (P=0.039). Group C showed the best efficacy in terms of clinical, dermoscopic, and RCM outcomes. ConclusionsAll three treatments improved the efficacy of PDT and were well tolerated, with cryotherapy + PDT showing the best efficacy.

Is the combination of cryotherapy and topical timolol solution superior to cryotherapy alone in the treatment of epidermal growth factor receptor inhibitor-induced paronychia? A double-blinded, left-to-right comparison, randomized controlled trial

Is the combination of cryotherapy and topical timolol solution superior to cryotherapy alone in the treatment of epidermal growth factor receptor inhibitor-induced paronychia? A double-blinded, left-to-right comparison, randomized controlled trial

TREATMENT OF KELOIDS AND HYPERTROPHIC SCARS BY COMBINED CRYOTHERAPY AND INTRALESIONAL TRIAMCINOLONE

Objectives: To evaluate the outcomes of combining cryotherapy and intralesional triamcinolone in the treatment of keloids and hypertrophic scars. Methods: 60 patients (31 males and 29 females) with 266 keloids and 34 hypertrophic scars were enrolled and divided equally into 2 groups. Group 1 were treated with cryotherapy in combination with intralesional triamcinolone 20 mg/ml (treated group) and group 2 (control group) were treated with intralesional triamcinolone alone 20mg/ml. The duration of treatment for both groups was 12 weeks with 4 sessions. The assessment of therapeutic responses was made at baseline, after 4, 8, 12 and 16 weeks. Results: After treatment, in terms of thickness, the keloids and hypertrophic scars responded significantly better to combined cryotherapy and triamcinolone than triamcinolone alone (p < 0.05): 1.3% had excellent improvement, 46.4% had good improvement and 41.2% had moderate improvement and 11.1% had little improvement. Meanwhile, in control group, the percentage for each degree of improvement were 1.4%, 16.7%, 55.6% and 26.4%, respectively. Ultrasound revealed an average reduction of scar height in treated group (2.4 ± 1.3 mm), which was higher than the result in control group (1.8 ± 0.4 mm) (p < 0.05). Side effects in treated group including pain, blister, hypopigmentation, longer healing time, acne, and troublesome with menstrual cycles were sometimes were reported. No significant side effects were reported in both groups. Conclusion: Combining cryotherapy with injection of intralesional steroids appeared to be superior in comparision with intralesional steroids alone in treatment of keloids and hypertrophic scars without severe side effects.

Efficacy of Cryotherapy Combined With Intralesional Purified Protein Derivative (PPD) Versus Intralesional PPD Monotherapy in the Treatment of Multiple Common Warts.

Intralesional antigen immunotherapy represents a promising therapeutic approach for the treatment of different types of warts, particularly if multiple and/or recalcitrant. to investigate the efficacy and safety of combined cryotherapy with intralesional purified protein derivative (PPD) immunotherapy in the treatment of multiple common warts. Fifty patients were randomly divided into two groups (25 patients each): Group A: receiving intralesional PPD immunotherapy for the largest wart, while group B: receiving cryotherapy for all warts plus intralesional PPD for the largest wart. Treatments were performed every 2 weeks for a maximum of four sessions. Photographs were taken at baseline and at each visit and clinical response was evaluated by the reduction in number and size of warts. Adverse effects were recorded. There was a significant reduction in size and number of warts in both groups (P < .001), with no significant difference between the two groups. Complete clearance of the lesions was observed in 48% of patients in group A and 44% in group B (P = .39). Higher rates of near complete/complete response were achieved after fewer sessions (2, 3 sessions) in group B (P = .002). Blistering was common after cryotherapy. Higher rate of hypopigmentation was noticed after combined treatment than after PPD monotherapy (56%, 8% respectively; P < .001), which resolved gradually. Both intralesional PPD alone and combined cryotherapy with PPD are safe and effective in clearing of common warts. Cryotherapy may be a successful adjunct to intralesional PPD immunotherapy that helps in reducing the number of treatment sessions.The study protocol was registered at ClinicalTrials.gov with ID: NCT04288817.

Combination cryotherapy and intralesional corticosteroid versus steroid monotherapy in the treatment of keloids.

Keloids are common and have significant negative effects on quality of life. There is a need for more effective treatment approaches for keloids. We investigated treatment outcomes of intralesional triamcinolone acetonide (IL TAC) compared with combination IL TAC and cryotherapy, including changes in pruritus, pain, and keloid size. We performed a prospective study of patients referred to one provider who treated patients with combination therapy and compared them to a historic control cohort treated with IL TAC alone. All patients were seen at Thomas Jefferson University between 2019 and 2021. Patient demographics, location of keloids, and inciting events were recorded. Pruritus and pain scores were self-reported by patients using a 10-point Likert scale administered as standard of care. Changes in keloid size were denoted as "No change," "up to 50% decrease," "more than 50% decrease," and "completely flattened." While both treatments produced a significant reduction in mean pruritus and pain scores, there was no difference between the two treatment groups (p=0.3933 and p=0.2123, respectively). A greater percentage of keloids in the combination therapy group had a post-treatment size difference greater than 50% compared with those in the IL TAC only treatment group (p=0.0021). In the subgroup of pubic keloids, all lesions treated with combination IL TAC and cryotherapy responded remarkably well to treatment. While both IL TAC and IL TAC with cryotherapy were effective at reducing pruritus and pain, combination therapy was more effective in reducing keloid size, specifically for pubic keloids.

Successful treatment of giant anogenital condyloma accuminata with combined cryotherapy and intralesional tuberculin purified protein derivative: a case report

To show the effectiveness of combined cryotherapy and intralesional tuberculin purified protein derivative (PPD) in the treatment of giant condyloma accuminata. A 57-year-old female was presented with an extensive verrucous, ulcerated, cauliflower-like 10 × 5 cm mass of the perianal area with extension to labia majora. The mass had evolved progressively for 2 years and resulted in perianal discomfort, itching and bleeding. Histopathological examination ruled out the presence of malignancy and confirmed the diagnosis of condyloma accuminata.The lesions were treated by 6 sessions of combined cryotherapy (2 freeze thaw cycles 25 seconds each) and intralesional PPD with a dose of (0.3 ml each session) at two-week interval. The mass resolved completely after 3 months and the quality of life of the patient has improved dramatically. There was no recurrence within 1 year of follow-up. Although most case reports suggest surgical excision as the treatment of choice of giant condyloma accuminata, our case was successfully treated with a highly effective and safe combined modality of cryotherapy and intralesional PPD, with the advantage of preserving the normal anatomy and avoidance of post-surgical disfigurement. No conflict of interest to declare.

Successful treatment of giant anogenital condyloma accuminata with combined cryotherapy and intralesional tuberculin purified protein derivative: a case report

Successful treatment of giant anogenital condyloma accuminata with combined cryotherapy and intralesional tuberculin purified protein derivative: a case report

Efficacy of Cryo-compression on Shin Splints in Female Amateur Marathon Runners

Background: Medial tibial stress syndrome (MTSS) or Shin splints is exercise-induced lower leg pain along the distal posterior-medial aspect of tibia caused by repetitive loading stress during running and jumping. It is one of the most common lower leg sports injury. Cryotherapy is the most commonly used treatment during immediate care of athletic injuries and acute soft tissue injuries and is also included as part of RICE (rest, ice, compression and elevation). Cryotherapy is helpful in reducing swelling as well as it exerts analgesic effect. The external compression is also used during immediate care phase and it helps in decrease tissue temperature and prevent oedema formation. Cryotherapy in combination with intermittent pneumatic compression hypothetically help in reducing the physiologic effects of traumatic tissue damage which is based on the models of cryotherapy and static compression therapy. This study aims to establish the efficacy of cryo-compression on shin splints in female amateur marathon runners. Methods: A total 30 subjects diagnosed with shin splints were selected for study. Subjects received cryo-compression for reducing pain and swelling and exercises to strengthen the muscles. Each session was conducted for 45 minutes duration, 5 days per week for 4 weeks. Outcome measure used was Numerical Pain Rating Scale (NPRS), Goniometry for ROM and Manual muscle testing (MMT). Results: According to statistical analysis, the results of the study demonstrate that there was a significant effect of the use of cryo-compression on numerical pain rating scale (p&lt;0.0001), range of motion (ankle plantarflexion, dorsiflexion, eversion and inversion) (p&lt;0.0001), manual muscle testing – ankle plantarflexors (p=0.0028) and ankle dorsiflexors (p&lt;0.0001) during the pre and post intervention assessment. Conclusion: The conducted study shows that efficacy of cryo-compression on shin splints in female amateur marathon runners is very effective in reducing pain, increasing range of motion (ROM) and strength of muscles along with other treatment program. KEYWORDS: Cryo-compression, shin splints, numerical pain rating scale (NPRS), range of motion, ankle plantarflexion, ankle dorsiflexion, ankle eversion, ankle inversion, manual muscle testing, ankle plantarflexors, ankle dorsiflexors.

ADDITION OF LOCAL CRYOTHERAPY FOR TREATMENT OF POST-MASTECTOMY LYMPHEDEMA

Worldwide, lymphedema can present as a significant health issue. Left untreated, it can have long-term medical and psychological consequences for patients. Cryotherapy is a new physical therapy modality used for many purposes including reduction of pain, inflammation, and edema. It is thought to decrease interstitial fluid volume through many mechanisms. Therefore, it is reasonable to think that cryotherapy might have a positive effect in treatment of lymphedema. The goal of this study was to investigate how local cryotherapy in combination with standard therapy affects patient outcomes. Forty post-mastectomy female patients aged 40-60 years old with lymphedema were referred to the outpatient clinics of the Faculty of Physical Therapy at South Valley University for medical treatment and follow-up by the vascular surgery department. Patients were randomly divided into two groups of equal size. Traditional physical therapy programs (manual lymphatic drainage, pneumatic compression, bandaging, breathing exercises, circulatory exercises, shoulder mobilizations, and ROM exercises) were combined with pulsed local cryotherapy three times per week for 12 weeks in Group (A). For 12 weeks, Group (B) received only traditional physical therapy three times per week. Patients were evaluated using circumferential measurement with tape at the wrist, below the elbow, and above the elbow level, as well as ultrasonography to assess skin thickness before the start of physical therapy, 6 weeks later, and at the end of the treatment (after 12 weeks). Results indicate that cryotherapy is an effective adjunct modality for the treatment of secondary lymphedema and should be added to physical therapy protocols for lymphedema rehabilitation.

Is topical 5-fluorouracil or combination 5-fluorouracil and cryotherapy more effective than cryotherapy alone for the treatment of actinic keratosis?

Is topical 5-fluorouracil or combination 5-fluorouracil and cryotherapy more effective than cryotherapy alone for the treatment of actinic keratosis?

Utility of different modalities in treatment of keloid scar

Background : Keloids constitute an abnormal benign fibro-proliferative wound healing response to any type of skin injury. it has high influence on Patient’s psychology and cosmetic view. It has high prevalence in African-American subjects. Until now there is no definitive treatment. The aim of study : to assess the response in three different groups using three different modalities (intralesional steroid, cryotherapy, and both). Patient and method : This was a randomized controlled clinical method with equivalent intervention assignment study, which was done on 16 patients (105 lesions), who attended Ibn Sina Teaching hospital, Mosul, Iraq, from January 2019 to January 2021. Results : there were significant responses to the three modalities (p-value less than 0.05). and the response assessed for each patient according to Vancouver Score Scale (VSS), Patient and Observer Scar Assessment Scale (POSAS), and 2 diameters assess. Conclusion: The results of this study showed that the combination of cryotherapy with intralesional steroids is more effective than steroid or cryotherapy alone in treating keloids.

Role of total cryotherapy in combination with transcranial magnet therapy in the change of microcirculation and psychological status in patients with menopausal syndrome

To study the effect of total cryotherapy alone and in combination with transcranial magnet therapy on the microcirculation state and psychological status in patients with MS. The study included 62 female patients (mean age was 51±1.9 years). Thirty-five (56%) patients had mild menopausal syndrome and 27 (44%) moderate severity. All patients were divided into two clinically and functionally comparable groups by simple randomization: Group 1: 30 patients who received total cryotherapy (TCT); Group 2: 32 patients who received complex treatment including TCT and transcranial magnet therapy. The blood lipid spectrum was studied in all patients before and after the treatment course. The psychological examination was carried out using the well-being/activity/mood test (WAM). The microcirculation state was assessed by laser Doppler flowmetry (LDF). The majority of the patients had abnormalities in the lipid spectrum, decreased psychological adaptation, and worsened microcirculation indices (according to LDF data). After a treatment course, improvement in the lipid spectrum, LDF, and WAM test scores according to all scales was more pronounced in group 2 patients receiving complex therapy. The combined effect of total cryotherapy and transcranial magnet therapy had a more pronounced positive impact on the lipid spectrum, psychoemotional state, and microcirculatory processes in patients with menopausal syndrome compared with the total cryotherapy alone. It can be assumed that this complex will be effectively used in practical healthcare.

Effects of Cryofrequence on Localized Adiposity: Case Study

Cryofrequence is the combination of cryotherapy with radiofrequency, which promotes a “thermal shock” to the tissue, working simultaneously to skin flaccidity and localized adiposity. The efficacy of dermatological and hypodermic cryofrequency treatment was evaluated. This study reports the case of a 45-year-old woman with complaints of flaccidity and fat located in the infra-umbilical region in the preoperative period of abdominoplasty. The evaluation was performed before and after treatment through evaluation protocols, ultrasound examinations and histological findings through optic microscopy, microfocal and immunohistochemical analysis. The treatment was performed in 4 sessions, with a weekly application, using the BHS 156 FULL®—BODY HEALTH BRASIL® apparatus, the parameters were temperature of -10°C, power in 70%, duration of 8 minutes. After the surgical procedure, parts of treated and untreated abdominal tissues were removed for evaluation. In the tests performed, significant destruction of adipocytes was detected, with consequent reduction of fat and greater expression of caspase-3 in the treated tissue, being constated the macrophage increase by CD68 expression. There was improvement in flaccidity, evidenced by the significant increase in the number of fibroblasts and inflammatory cells positive for Ki67, bringing more quantity and better quality to the collagen.

Efficacy of Gas Combination Cryotherapy in the Management of Odontogenic Keratocyst of the Maxilla and Mandible: A Pilot Study.

The aim of this study was to evaluate outcome of management of odontogenic keratocyst (OKC) using gas combination cryotherapy (GCC). GCC is a treatment modality where cyst enucleation is followed by applying an adjuvant agent, a spray containing propane, butane and isobutene gas onto the bony bed. This was a prospective interventional study which included patients with radiographic and histopathologic evidence of OKC. All patients underwent enucleation of the cysts followed by spraying of the bony cavity with "ENDOFROST ™"(commercially available agent for GCC). Patients were periodically reviewed and the presence of wound dehiscence, infection and neurosensory deficit was noted. After 15months, a CBCT was taken to assess the amount of bone formation, the presence of any pathologic fracture or recurrence. Comparison of the preoperative and the postoperative size of the defect was also assessed. A total of 10 patients were included in the study of which only 2 presented with neurosensory deficit in the post-op period. One patient of the two patients recovered completely within 12months, while the other recovered within 15months. There was no evidence of any patient developing infection, wound dehiscence, recurrence or pathologic fracture. The results of the study show that enucleation followed by GCC is a safe, effective and low-cost therapy for the management of OKC.

COMPARATIVE STUDY OF EFFICACY OF CRYOTHERAPY AND MYOFASCIAL RELEASE TECHNIQUE IN CALF MUSCLE SPASTICITY IN SPASTIC DIPLEGIC CEREBRAL PALSY CHILDREN

The greatest substantial consequence of juvenile impairment is cerebral palsy (CP). Spasticity of numerous muscle groupings is common after Central Nervous System damage. Spasticity causes functioning issues. For individuals suffering from CP ankle anomalies seem strongly tied to workable limits. Spastic CP is highest frequent type of CP, which limits child's responsive status and prohibits them from participating in communal activities. Soft tissue mobilisation procedures would be used in variety ways. Cryotherapy is interventional method for stiffness in neurological patients that has narrow advantage. MFR can potentially utilised against spasticity. The focus of this investigation is to evaluate Cryotherapy and MFR influence over calf muscle stiffness in participants who have Spastic Diplegic CP. The survey's goal is to determine efficacy of Cryotherapy and MFR in calf muscle spasticity in Spastic diplegic CP subjects. Thirty individuals suffering spastic diplegic CP were chosen using randomized sampling procedure. The subjects was categorised onto 3 subgroups, each of ten individuals. Cryotherapy was applied to Group A, MFR was applied to B and C Group was applied with combination of cryotherapy and MFR. Both before and after therapy, MAS and goniometry are used to assess calf muscle spasticity and ankle PROM. Overall validity of MAS and PROM comparing before and after in subgroup was determined using Tukey Test. Spasticity was reduced and range of motion was improved (p&lt;0.05) in intra group comparison. Cumulative influence of cryotherapy and MFR is beneficial in reducing calf muscle stiffness in spastic diplegic CP children, as per this investigation.

Effects of Cryofrequency at Dermal and Hypodermic Level: Case Study

Background: Cryofrequency consists of the combination of cryotherapy and radiofrequency, acting simultaneously in the treatment of flaccidity and localized adiposity. Objective: To investigate the effects of cryofrequency at the dermal and hypodermic levels. Materials and methods: This is a case study, composed of one woman of 39-year-old, with interest to perform abdominoplasty surgery, presenting adiposity and flaccidity located in infra-umbilical region. Its treatment consists of eight cryofrequence applications. Results: There was reduction of 7 cm on supra-umbilical plicometry and 6 cm on infra-umbilical; a decrease of 2.7 kg of body weight. The ultrasound data show a decrease of 0.6 mm, in the thickness of the adipose layer. In the histological analysis: thicker epidermis, with more layers and dermis with greater number of fibroblasts and inflammatory cells, greater quantity of neoformed collagen tissue. Conclusion: The application of cryofrequence promoted reduction of adipose tissue and increased production of collagen. Level of Evidence: Level III, case-control study.