Separation Column Research Articles

Benchmarking Hydrogen Isotope Separation Efficiency of Pd/k–Packed TCAP Columns

By leveraging the large isotope effect in the palladium hydrogen isotope system, the Thermal Cycling Absorption Process (TCAP) provides an efficient and advantageous means to separate protium, deuterium, and tritium. To meet increased future tritium processing demands, such as those needed for fusion power plants, current designs of the separation columns need to be adapted and optimized using the progress made in understanding hydrogen isotope science. One key to this optimization lies in understanding the baseline performance for currently employed separation packing materials. Pd/k and molecular sieves, as commonly used for the separation of hydrogen isotopes, are herein evaluated to establish a baseline for their separation efficiency. Van Deemter plots are formulated, and the influence of each parameter is evaluated to determine areas for improvement.

Processing algorithm of weekly records of the Roztochia landscape-geophysical station thermograph М-16АН as a response source of air temperature data

Formulation of the problem. During the database processing of the Roztochia landscape-geophysical station (RLGS), located in the village of Bryukhovychi, Lviv, an air temperature data gap for 1990–1991 was found. The task of the research was to find those sources about air temperature at RLGS, which would allow us to fill in the gaps during the night hours when, unfortunately, observers did not make measurements. Problems of further research. In comparison with the method adopted in Ukraine for processing weekly thermograph tapes, in this study, it is proposed to correct the air temperature value during their processing, compensating for accelerated or slowed rotation of the weekly thermograph drum. It is suggested to use only those dry bulb measurements carried out on days with cloudy or rainy weather. The purpose. The main goal was to find an algorithm for processing weekly thermograph tapes under the conditions of a partial absence of temperature measurements using a dry thermometer by an observer to fill in the gaps regarding night air temperature data. Research methods. The air temperature values falling during the measurement period were read from the thermograph tapes, and an electronic table was formed. The temperature values (difference estimation) were compared with the corresponding ones recorded in the "Books" of KM-1. At the same time, it was necessary to make two new corrections. The first correction will be made along the ordinate axis, changing the value of the temperature recorded by the thermograph compared to the values of the temperature measured by the dry thermometer. The second correction was made along the abscissa axis, compensating for the drum's slowed down or accelerated rotation. Presentation of the main research material. A brief description of the proposed algorithm for thermograph tape processing is as follows. In the spreadsheet, in separate columns, we record the temperature values during the observation periods: a) by dry thermometer and b) by thermograph at the points corresponding to the observation periods. Subtracting columns (a) and (b) values, we determine those dry bulb temperature values suitable for calculating corrections. We reject too significant differences that occur during rapid temperature changes. Next, we look for points on the thermograph tape that serve as time benchmarks (the starting and ending points of the temperature curve and the places where the observer draws vertical lines). These temperature values will form column (c). It will additionally include the temperature values obtained by reading the temperature from the tape for rainy and/or overcast days for points whose localization is corrected for time. The difference between column (a) and column (c) will give the temperature correction for several observation periods on each weekly strip taken separately. The last step is the linear interpolation of temperature corrections between neighbouring points of intermediate observation periods. Practical value. The proposed algorithm may help eliminate gaps in temperature data at other observation points, where the thermograph served as a backup device for recording air temperature. Research results. The measurements that fall on rainy and overcast weather are best suited for calculating thermograph corrections when air temperature changes slow down. It is necessary to identify benchmark points of time fixation, to which the observer must add the moments of putting on and removing the tape from the drum.

Environmental and molecular regulation of asexual reproduction in the sea anemone Nematostella vectensis

Cnidarians exhibit incredible reproductive diversity, with most capable of sexual and asexual reproduction. Here, we investigate factors that influence asexual reproduction in the burrowing sea anemone Nematostella vectensis, which can propagate asexually by transverse fission of the body column. By altering culture conditions, we demonstrate that the presence of a burrowing substrate strongly promotes transverse fission. In addition, we show that animal size does not affect fission rates, and that the plane of fission is fixed along the oral–aboral axis of the polyp. Homeobox transcription factors and components of the TGF β , Notch, and FGF signalling pathways are differentially expressed in polyps undergoing physal pinching suggesting they are important regulators of transverse fission. Gene ontology analyses further suggest that during transverse fission the cell cycle is suppressed, and that cell adhesion and patterning mechanisms are downregulated to promote separation of the body column. Finally, we demonstrate that the rate of asexual reproduction is sensitive to population density. Collectively, these experiments provide a foundation for mechanistic studies of asexual reproduction in Nematostella, with implications for understanding the reproductive and regenerative biology of other cnidarian species.

Rhizosphere nutrient availability and nutrient uptake of soybean in response to biochar application

Biochar application in agricultural soils can be highly beneficial to crop productivity in numerous ways. Rhizosphere nutrient dynamics and its relation to nutrient uptake of crops under biochar amendment remain unclear. Therefore, the present study investigated the combined effect of biochar application and incubation on growth, yield, and rhizosphere nutrient availability of soybean (Glycine max L.) plant. A pot experiment was conducted with two biochar applications (with and without biochar) and two incubation conditions (with and without incubation) in a glasshouse. A separate soil column leaching experiment was conducted to test the nutrient retention ability of soil once biochar is amended. Irrespective of the incubation condition, biochar application made a significant increase in pod dry weight. The total nutrient uptake by soybean greatly increased under biochar amendment compared with the plants grown without biochar. When incubation is not done, soybean increased its P and K uptake respectively by 52% and 48% under biochar amendment compared to those without biochar. With incubation, the relevant increases were 42% and 32% respectively. The results from soil column leaching experiment suggest that the application of biochar in planting media could be an effective way to retain soil N by reducing the leaching losses.

Energy analysis and optimization of heat integrated air separation column based on non-equilibrium stage model

A large scale of nitrogen and oxygen production has been through a cryogenic air separation process. This process takes place in a distillation column at excessive energy consumption. The heat-integrated air separation column (HIASC) can save energy when utilizing minimum pressure while ensuring adequate heat distribution along the portion of the column. However, accurate prediction of thermodynamic and fluid properties requirement is critical for process optimization and potential energy saving. The nonequilibrium stage model is employed to gain insight into the thermodynamic properties. While examining the influence of these properties, essential process operating variables are optimized to save energy. The research result shows that more than 17% of energy is preservable in the HIASC.

A magnetic nanoparticles–based method for nucleic acid extraction from specimen collection

Nucleic acid (NA) can be utilized as a bio-marker for the detection of infectious disease. NA often presence in a low concentration in a matrix which is one of the major limitations for the utilization of NA. A preconcentration including column separation or centrifugation was used for the separation NA. Another method is to use surface-improved magnetic beads to bind to NA and then separate nucleic acids from their unused parts under an external magnetic field and using of magnetic beads is considered a fast and safe method. Therefore, we are interested to increase the concentration of NA in sample by using methacrylic acid and polyethyleneimine modified magnetic nanoparticles for nucleic acid extraction from samples to study the capturing efficiency of modified MNPs to nucleic acid.In this work we synthesize iron oxide nanoparticles (Fe3O4 NPs) in 4 sizes (11.56 ± 1.17 nm,9.40 ± 1.18 nm,14.07 ± 1.98 nm and 13.98 ± 3.07 nm) by a simple co-precipitation and coat with methacrylic acid on the largest size (14.07 ± 1.98 nm) by the classical radical before polyethyleneimine modification to increase positively charge on the Fe3O4 NPs surface for NA extraction from samples to reduce the process of measuring the infection.

Separation and identification of different therapeutic pharmaceutical drugs on humic acid-based HPLC column: Method optimization using central composite design.

Multimode chromatographic separations are highly desirable in pharmaceutical and environmental sciences. Current study deals with the application of newly developed mixed-mode end capped-immobilized humic acid onto an aminopropyl silica based chromatographic column for separation and identification of six drugs belonging to different therapeutic groups for its applicability in pharmaceutical industries. For this, central composite design was used to evaluate the separation and resolution by optimization of three most effective parameters (acetonitrile%, flow rate, and pH of mobile phase). Second-order quadratic model was used to evaluate their effect on resolution of peaks; the probability value (<0.05) obtained from analysis of variance suggested the best applicability of the model. Desirability function was applied to calculate optimum conditions (44.8% acetonitrile, 1.75mL/min of flow rate, and 7.5pH) required to achieve maximum separation with good resolution within 11 min. The method was validated for linearity, precision accuracy, selectivity, and sensitivity. The results revealed a highly precise (coefficient of variance>1%), linear (R2 =0.99), and highly selective method. Moreover, the limit of detection/quantification values revealed acceptable sensitivity of the method. The developed column was compared for its efficiency with a commercially available column and found to be highly applicable for industrial applications.

Preparation of β-cyclodextrin covalent organic framework-immobilized poly(glycidyl methacrylate) nanoparticle-coated open tubular capillary electrochromatography column for chiral separation.

A new enantioselective open-tubular capillary electrochromatography was developed employing poly(glycidyl methacrylate) nanoparticles/β-cyclodextrin covalent organic frameworks chemically immobilized on the inner wall of the capillary as a stationary phase. A pretreated silica-fused capillary reacted with 3-aminopropyl-trimethoxysilane followed by poly(glycidyl methacrylate) nanoparticles and β-cyclodextrin covalent organic frameworks through a ring-opening reaction. The resulting coating layer on the capillary was characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. The electroosmotic flow was studied to evaluate the variation of the immobilized columns. The chiral separation performance of the fabricated capillary columns was validated by the analysis of the four racemic proton pump inhibitors including lansoprazole, pantoprazole, tenatoprazole, and omeprazole. The influences of bonding concentration, bonding time, bonding temperature, buffer type and concentration, buffer pH, and applied voltage on the enantioseparation of four proton pump inhibitors were investigated. Good enantioseparation efficiencies were achieved for all enantiomers. In the optimum conditions, the enantiomers of four proton pump inhibitors were fully resolved within 10min with high resolutions of 9.5-13.9. The column-to-column and inter- to intra-day repeatability of the fabricated capillary columns through relative standard deviation were found better than 9.54%, exhibiting satisfactory stability and repeatability of the fabricated capillary columns.

Remote Monitoring the Parameters of Interest in the 18O Isotope Separation Technological Process

This manuscript presents the remote monitoring of the main parameters in the 18O isotope separation technological process. It proposes to monitor the operation of the five cracking reactors in the isotope production system, respectively, the temperature in the preheating furnaces, the converter reactors and the cracking reactors. In addition, it performs the monitoring of the two separation columns from the separation cascade structure, respectively, the concentrations of the produced 18O isotope and the input nitric oxides flows. Even if the production process is continuously monitored by teams of operators, the professionals who designed the technical process and those who can monitor it remotely have the possibility to intervene with the view of making the necessary adjustments. Based on the processing of experimental data, which was gathered from the actual plant, the proposed original model of the separation cascade functioning was developed. The process computer from the monitoring system structure runs the proposed mathematical model in parallel with the real plant and estimates several signal values, which are essential to be known by the operators in order to make the appropriate decisions regarding the plant operation. The separation process associated with the final separation column from the separation cascade structure is modeled as a fractional-order process with variable and adjustable differentiation order, which represents another original aspect. Neural networks have been employed in order to implement the proposed mathematical model. The accuracy, validity and efficiency in the operation of the proposed mathematical model is demonstrated through the simulation results presented in the final part of the manuscript.

Development of a HPLC system using a phase-separation multiphase flow as an eluent coupled to a silica-particle packed column.

The study reports the development of a high-performance liquid chromatography (HPLC) system in which a phase-separation multiphase flow as the eluent and a silica-particle based packed column as the separation column were combined to form the phase separation mode. Twenty-four types of mixed solutions of water/acetonitrile/ethyl acetate and water/acetonitrile were applied as eluents to the system at 20°C. 2,6-Naphthalenedisulfonic acid (NDS) and 1-naphthol (NA) were injected as model analytes into the system. They showed separation tendency in organic solvent-rich eluents in normal-phase mode and NA was detected earlier than NDS. Subsequently, seven types of the ternary mixed solutions were examined as eluents in the HPLC system at 20°C and 0°C. These mixed solutions worked as a two-phase separation mixed solution, providing a phase-separation multiphase flow at 0°C in the separation column. In the organic solvent-rich eluent, the mixture of analytes was separated at both 20°C (normal-phase mode) and 0°C (phase-separation mode), with NA being detected earlier than NDS. The separation at 0°C was more efficient than at 20°C. In the water-rich eluent, the mixture of NDS and NA was not separated at 20°C but was separated at 0°C (phase-separation mode), with NDS being detected earlier than NA. We also discussed the separation mechanism of phase-separation mode in HPLC together with the computer simulation for the multiphase flow in the cylindrical tube having sub-μm inner diameter.

Water column separation under one-after-another load rejection in pumped storage station

A pumped storage power station operates on the principal of multiple pump-turbines sharing one tunnel. However, safety is a concern, particularly under one-after-another load rejection of multiple pump-turbines. In this study, we have implemented a model experiment based on water column separation experiment under the condition of one-after-another load rejection of a pumped storage model experimental platform with two pump-turbines sharing one tunnel. The results show that the unit with water column separation, which is Unit # 2, has the worst moment when it rejects full load 0.8 s after Unit #1. At this condition, the wall pressure of the draft tube of unit # 2 attains the vapor pressure and reverse water hammer pressure attains the maximum value of 6.8 m during water column bridging. However, the reverse water hammer pressure was 290 m in prototype unit. Under one-after-another load rejection, water column separation occurs when the draft tube attains the second extreme value, and the unit located in the turbine braking condition area. It was observed that the pressure pulsation frequency of the draft tube after one-after-another load rejection is composed of two parts. First, pressure pulsation frequency caused by swirling flow with a value of 0.4 f/fn and second, a broadband of 40–200 Hz generated by the reverse water hammer due to water column bridging and structural response of the unit.

Development and validation of an LC-MS/MS method for simultaneous determination of three organic azido impurities in tetrazole-containing sartans

This study aimed to develop a sensitive and simple liquid chromatography-tandem mass spectrometry for the simultaneous determination of organic azido impurities (AZBC, AZBT, and AZTT) in sartan active pharmaceutical ingredients and drug products. The method employed a HALO C8 column for chromatographic separation using gradient elution, with a mobile phase composed of 0.1% formic acid in water and 0.1% formic acid in acetonitrile. Quantification of analytes was achieved using a triple quadrupole mass spectrometer, operating in multiple reaction monitoring mode with positive electrospray ionization. The method was fully validated following the ICH Q2 (R1) guideline, and the validation parameters met the criteria for specificity, carryover, and robustness. The developed method was found to be sufficiently sensitive, with LOD and LOQ below the acceptable limits of azido impurities in pharmaceuticals as per the ICH M7 guideline. The assay had dynamic ranges of 1.00–20.00 ng/mg for AZBC and 0.10–15.00 ng/mg for AZBT and AZTT in sartans. In conclusion, the established method was effectively utilized for determining azido impurities in sartan active pharmaceutical ingredients and drug products, achieving high accuracy and precision.

Evaluation of Kinetic Performance of Reversed-Phase Columns for Protein Separations by Gradient Kinetic Plots

The increasing importance of protein biopharmaceuticals has triggered the development of new, highly efficient stationary phases for reversed-phase liquid chromatography (LC) of proteins. They typically have C4 ligands or phenyl surfaces for weak hydrophobic interactions and are based on various morphologies, such as silica monolith, sub-2-µm fully porous particles (FPPs), or superficially porous particles (SPPs). Selection of the best column based on physical parameters provided by vendors may sometimes be unequivocal. Simple performance evaluation tools, such as the gradient kinetic plot methodology reported by Desmet and co-workers, can help to shed light on this issue and allow for a more differentiated view on column performance. This article compares the performance of wide-pore silica monolithic, sub-2-µm FPP, and SPP columns. It also addresses the question of whether 1000 Å or 400 Å SPP columns are more suitable for reversed-phase LC-type protein separations and presents a kinetic performance comparison of a number of wide-pore core–shell particle columns.

Facilitating method development for reverse fill/flush flow modulation by using a tuneable auxiliary pressure source instead of a fixed bleed capillary.

The conventional reverse fill/flush flow modulation for comprehensive two-dimensional gas chromatography requires a bleed capillary column to be connected to the outlet of the modulator channel. The purpose of this capillary, which does not contain the stationary phase, is to provide pressure resistance to the modulator channel flow. In this way, the desired modulator volumetric flow can be achieved, and channel over-filling can be avoided. Normally, the length and the internal diameter of the bleed capillary are chosen so as to obtain the modulator flow that is close to the flow of the first separation column. Thus, for any chosen set of chromatographic conditions, the required dimensions of the bleed capillary can be completely different, making the two-dimensional gas chromatography method development tedious and generating additional costs in consumables and analyst time. In this work, a tuneable pressure source generating a suitable backpressure was used instead of the fixed bleed capillary which has the advantage of the possibility to freely adapt the pressure resistance and generate the required modulator channel flow for any conditions. This set-up has been evaluated and compared in terms of the impact on the modulation performance to the set-up involving a fixed bleed capillary demonstrating comparable performance.

Cuscuta reflexa Possess Potent Inhibitory Activity Against Human Malaria Parasite: An In Vitro and In Vivo Study

Drug resistance to practicallyallantimalarialdrugs in use necessitate the development of new chemotherapeutics against malaria. In this aspect, traditionally used plants with folklore reputation are the pillar for drug discovery. Cuscuta reflexa being traditionally used in the treatment of malaria in Odisha, India we aimed to experimentally validate its antimalarial potential. Different solvent extracts of C. reflexa or column fractions from a promising solvent extract were evaluated for in vitro anti-plasmodial activity against Plasmodium falciparum strain Pf3D7. Potent fractions were further evaluated for inhibition of parasite growth against different drug resistant strains. Safety of these fractions was determined by in vitro cyto-toxicity, and therapeutic effectiveness was evaluated by suppression of parasitemia and improvement in survival of experimental mice. Besides, their immunomodulatory effect was investigated in Pf-antigen stimulated RAW cells. GCMS fingerprints of active fractions was determined. Column separation of methanol extract which showed the highest in vitro antiplasmodial activity (IC50 = 14.48μg/ml) resulted in eleven fractions, three of which (F2, F3, and F4) had anti-plasmodial IC50 ranging from ≤ 10 to 2.2μg/ml against various P. falciparum strains with no demonstration of in vitro cytotoxicity. F4 displayed the highest in vivo parasite suppression, and had a mean survival time similar to artesunate (19.3 vs. 20.6days). These fractions significantly modulated expression of inflammatory cytokines in Pf-antigen stimulated RAW cells. The findings of the study confirm the antimalarial potential of C. reflexa. Exploration of phyto-molecules in GCMS fingerprints of active fractions is warranted for possible identification of lead anti-malarial phyto-drugs.

A Novel Two-Dimensional Liquid Chromatography Combined with Ultraviolet Detection Method for Quantitative Determination of Pyridoxal 5'-Phosphate, 4-Pyridoxine Acid and Pyridoxal in Animal Plasma.

Vitamin B6 is an indispensable micronutrient in organisms and is widely distributed in blood, tissues, and organs. Changes in the content and ratio of vitamin B6 can affect the entire physiological condition of the body, so it becomes particularly important to reveal the relationship between changes in its content and disease by monitoring vitamin B6 levels in the organism. In this study, a two-dimensional liquid chromatography-UV detector (2D-LC-UV) was used to establish a method for the simultaneous detection of PLP, PA, and PL for the first time. First, PLP, PA, and PL were extracted with plasma: 0.6 M TCA: ultrapure water = 1:2:3 (v/v/v) and then derivatized. Enrichment and preliminary separation were performed on a one-dimensional column and automatically entered into a two-dimensional column for further separation. This method exhibited good selectivity, and the correlation coefficients for the analyte calibration curves were >0.99. The detection limits for PLP, PA, and PL were 0.1, 0.2, and 4 nmol/L, respectively. The results showed that the system has high loading capacity, excellent resolution, and a good peak shape. This method is expected to provide applicability for the determination of PLP, PA, and PL in pharmacological, pharmaceutical, and clinical research.

Startup methods analysis of a cryogenic distillation system with two interlinked columns for hydrogen isotope separation

Effective startup methods are crucial for the successful operation of a distillation facility. In order to design a viable startup method for a cryogenic distillation demonstration system with two interlinked columns for hydrogen isotope separation, which is under construction at Institute of Materials, China Academy of Engineering Physics, Aspen Plus and Aspen Dynamics were employed in combination to generate a dynamic numerical model for the startup analysis. Total four conventional startup methods were analysed. The most time-effective method was identified and the other of the four startup methods were found to be able to lead to successful startup results but with varied operation complexity and length of time to reach the steady state. The effective startup method that involved in charging the two distillation columns with equilibrated hydrogen isotope mixtures respectively, took only 50.36 h to reach the target steady state. And the identified method would be employed into the cryogenic distillation demonstration system to check its startup efficient.

An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure for the quantification of levetiracetam in human serum and plasma.

To develop an isotope dilution-liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based candidate reference measurement procedure (RMP) for levetiracetam quantification in human serum and plasma. Quantitative nuclear magnetic resonance spectroscopy (qNMR) was used to characterize the RMP material to ensure traceability to SI units. To quantify levetiracetam, an LC-MS/MS method was optimized using a C8 column for chromatographic separation following protein-precipitation-based sample preparation. Spiked matrix samples of serum and plasma were used to test selectivity and specificity. Matrix effects were determined by performing a post-column infusion experiment and comparing standard line slopes. Precision and accuracy were evaluated over 5days. Measurement uncertainty was evaluated according to the Guide to the Expression of Uncertainty in Measurement (GUM). The RMP was proven to be highly selective and specific with no evidence of a matrix effect, allowing for quantification of levetiracetam within the range of 1.53-90.0μg/mL. Intermediate precision was <2.2% and repeatability was 1.1-1.7% across all concentrations. The relative mean bias ranged from-2.5% to-0.3% across all levels and matrices within the measuring range. Diluted samples were found with a mean bias ranging from-0.1 to 2.9%. The predefined acceptance criterion for measurement uncertainty was met and determined for individual measurements independently of the concentration level and sample type to be≤4.0% (k=2). We present a novel LC-MS/MS)-based candidate RMP for levetiracetam in human serum and plasma. Its expanded measurement uncertainty of≤4.0% meets the clinical needs in levetiracetam monitoring. Utilizing qNMR to characterize levetiracetam reference materials allowed metrological traceability to SI units.

Preparation of Chiral Porous Organic Cage Clicked Chiral Stationary Phase for HPLC Enantioseparation.

Porous organic cages (POCs) are a new subclass of porous materials, which are constructed from discrete cage molecules with permanent cavities via weak intermolecular forces. In this study, a novel chiral stationary phase (CSP) has been prepared by chemically binding a [4 + 6]-type chiral POC (C120H96N12O4) with thiol-functionalized silica gel using a thiol-ene click reaction and applied to HPLC separations. The column packed with this CSP presented good separation capability for chiral compounds and positional isomers. Thirteen racemates have been enantioseparated on this column, including alcohols, diols, ketones, amines, epoxides, and organic acids. Upon comparison with a previously reported chiral POC NC1-R-based column, commercial Chiralpak AD-H, and Chiralcel OD-H columns, this column is complementary to these three columns in terms of its enantiomeric separation; and can also separate some racemic compounds that cannot be separated by the three columns. In addition, eight positional isomers (iodoaniline, bromoaniline, chloroaniline, dibromobenzene, dichlorobenzene, toluidine, nitrobromobenzene, and nitroaniline) have also been separated. The influences of the injection weight and column temperature on separation have been explored. After the column has undergone multiple injections, the relative standard deviations (RSDs) for the retention time and selectivity were below 1.0 and 1.5%, respectively, indicating the good reproducibility and stability of the column for separation. This work demonstrates that POCs are promising materials for HPLC separation.

Equine Spermatozoa Selection by Magnetic Activation for Use in Assisted Reproduction.

This study aimed to select high-quality spermatozoa by sperm separation by magnetic activation of the fresh equine semen, compared to density gradient centrifugation and evaluating cell quality after selection. The semen of 10 stallions was collected by the artificial vagina technique. The samples analyzed were: (1) fresh semen; (2) density gradient centrifugation (DGC); (3) separation by magnetic activation (MASS) (nonapoptotic portion NAP); (4) separation by MASS (apoptotic portion-APT). Was analyzed: motility (light microscopy), concentration (Neubauer chamber), semen morphology (humid chamber in phase contrast), and supravital test (eosin/nigrosine). In DGC, 20×106 spermatozoa were used in the gradient of Percoll at 90% and 45% (400 μL each), centrifugation at 900 G/5 min, the pellet was diluted in HEPES. In MASS, 10×106 spermatozoa were diluted in 1.5 mL of HEPES, centrifugation at 300 G/10 min, pellet was resuspended in 150 μL of HEPES with 20 μL of nanoparticles bound to annexin V, incubation for 15 minutes and filtered in the magnetic separation column. The nonapoptotic fraction was collected directly and the apoptotic fraction after removal the column from the magnet and adding 300 μL of HEPES. The total abnormalities were 43.2% ± 2.78%, with the DGC and MASS being effective in reducing sperm abnormality by 15.6% ± 2.10% and 24.30% ± 1.63%, respectively, like the observed for the number of cells with intact membranes (50% lower in the APT portion). This nanotechnological method is efficient in producing high-quality semen samples for assisted reproduction procedures.