Column Chromatographic Method Research Articles

Evaluation of Bioactive Metabolites Isolated from Endophytic Fungus Chaetomium cupreum of the Plant Mussaenda luteola

Abstract: Background: Endophytic fungi are one of the most promising resources of natural bioactive products. In the present study, secondary metabolites were isolated from the endophytic fungus Chaetomium cupreum and evaluated in vitro for pharmaceutical potentials. Objective: To characterize the secondary metabolites obtained from the endophytic fungus Chaetomium cupreum from the plant Mussaenda luteola and to determine its antioxidant, cytotoxicity and anti-mycobacterial activities. Methods: Three secondary metabolites were isolated by column chromatography and solvent-solvent fractionation method from the ethyl acetate and methanol extract of C. cupreum. The structures were elucidated by spectroscopic studies includes FT-IR, NMR, MS analysis. Anti-mycobacterial activity of metabolites was determined by Microtitre Plate Alamar Blue Assay (MABA) against Mycobacterium tuberculosis H37Rv (ATCC27294) and also evaluated the antioxidant potentials using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. The cytotoxicity test was performed against breast cancer cell line MCF-7 using MTT assay. Results: Three secondary metabolites were characterized as 6- (heptacosa- 18ˈ Z enyl)- 2- (- 18ˈˈ hydroxyl- 1ˈˈ enyl- 19ˈˈ oxy)- 3 hydroxy benzoquinone (1), (3β –5α– dihydroxy –6β– phenyl acetyloxy – ergosta –7, 22 – diene) (2) and 2- dodecanol (3). The results of bio-assays revealed that the compound (1) and (2) exhibit significant inhibition of mycobacterium with MIC of 6.25μg/ml and 25μg/ml respectively, which is similar to the standard streptomycin drug. It also exhibit good DPPH scavenging potential of 72.07±1.95 and 71.63±1.40 % respectively. The cytotoxicity was tested against breast cancer cell line MCF-7 and found compound 1 and 2 had cytotoxicity of 52% and 49% respectively at 100μg/mL. Conclusion: The present study indicated that the isolated secondary metabolites from the endophytic fungus C. cupreum could be a potential lead molecule of antimicrobial agents. Key words: Endophytic fungi, Chaetomium cupreum, Secondary metabolites, Antimycobacterial, Antioxidant.

Senyawa Triterpenoid dari Ekstrak N-heksana Daun Kelor (Moringa oleifera Lamk.) dan Uji Aktivitas Antibakteri Terhadap Staphylococcus aureus dan Escherichia coli

Kelor plant (Moringa oleifera Lamk) is a plant that grows naturally in a tropical climate. During this decoction of pirdot leaves plants are used by the people as antimicroba. The purpose of this study was to isolate triterpenoid compound from ethyl acetate extract of kelor leaves. The sample extraction was performed by maceration method using methanol as a solvent. Fractionation used n-hexana and etil asetat as a solvent. The separation and purification of the compound was carried out by column chromatography method followed by stain pattern analysis with thin layer chromatography (TLC). Characterization and elucidation of pure compound structures using UV, IR, 1H-NMR, 12C-NMR, HSQC, HMBC, and 1H-1H COSY, and compared with various literatures. Based on the result of structural elusidation, the pure isolate obtained is a pentacyclic triterpenoid group compound with the molecular formula C30H50O and the name of 3-hydroxy, 20 (29) -en, lupenol. The triterpenoid compound of 3-hidroxy, 20 (29) -en, lupenol is the first isolated and reported from leaves of kelor plant.

Facile one-pot synthesis of novel highly functionalized dihydro-1H-pyrrole derivatives catalyzed by molecular iodine

An efficient, environmentally benign and molecular iodine promoted protocol was described for cascade synthesis of two kinds of novel dihydro-1H-pyrrole derivatives, 5 and 7. Compounds 5 were prepared via four-component reaction between diethyl acetylenedicarboxylate (DEAD), aromatic amines and phenylglyoxal monohydrate catalyzed by 10 mol% of iodine in ethanol at room temperature. In addition, using same reaction conditions, a three-component reaction between (E)-N-methyl-1-(methylthio)-2-nitroethenamine (NMSM), aromatic amines and arylglyoxal monohydrate was performed to access product 7. These transformations apparently proceed through an imine intermediate followed by iodine induced Mannich reaction and subsequent intra-molecular cyclization sequences. The protocol involves the formation of CN, CC and OH bonds leading to the preparation of a hexa substituted five membered ring with two stereo centres. The syntheses avoid the use of traditional column chromatography and recrystallization purification methods and, hence, follow the group-assisted purification (GAP) chemistry process.

Flavonoids from Artemisia rupestris and their synergistic antibacterial effects on drug-resistant Staphylococcus aureus

This study seeks to discover flavonoids from a traditional Chinese herb, Artemisia rupestris L., with synergistic antibacterial effects against multidrug-resistant Staphylococcus aureus. Five flavonoids, artemetin (1), chrysosplenetin (2), pachypodol (3), penduletin (4) and chrysoeriol (5) were obtained by various column chromatographic methods. Their chemical structures were determined on the basis of comprehensive spectroscopic analysis and comparison with literature data. Three of the compounds (2, 4 and 5) exhibited synergistic activity when combined with norfloxacin against SA1199B, an effluxing fluoroquinolone-resistant strain. The fractional inhibitory concentration indices (FICIs) of 2, 4 and 5 in combination with norfloxacin were 0.375, 0.079 and 0.266 respectively, suggesting synergy. Compound 5 also showed synergistic effects against EMRSA-15 and EMRSA-16 when combined with ciprofloxacin and oxacillin exhibiting FICIs of 0.024 and 0.375 respectively. Real time ethidium bromide (EtBr) efflux assay, qRT-PCR and molecular docking were employed to explore the mechanisms of the synergistic effects.

Zerumbone and Kaempferol Derivatives from the Rhizomes of Zingiber montanum (J. Koenig) Link ex A. Dietr. from Bangladesh

Zingiber montanum (J. Koenig) Link ex A. Dietr. (Zingiberaceae) is known as “Banada” in Bangladesh, and the rhizomes are frequently used in traditional medicines for the treatment of constipation, dyspepsia, flatulence, stomach bloating, and as mosquito repellant. In this study, dried rhizomes were extracted successively with 95% and 50% ethanol and the combined extract was then subjected to various column chromatographic methods to isolate one sesquiterpenoid derivative, zerumbone (1) and five kaempferol derivatives, i.e., kaempferol 3-O-methyl ether (2), kaempferol 3-O-α-rhamnopyranoside (3), kaempferol 3-O-α-(4”-O-acetyl)rhamnopyranoside (4), kaempferol 3-O-α-(3”-O-acetyl)rhamnopyranoside (5), and kaempferol 3-O-α-(3”,4”-di-O-acetyl)rhamnopyranoside (6). All compounds except 1 were isolated for the first time from the title plant.

Antioxidant and antimicrobial activities of (6E, 10E)-2, 6, 24-trimethyl pentacosa-2, 6, 10-triene from Euclea crispa leaves

The purpose of the present study attempt to investigate the antioxidant and antimicrobial activities of isolated bioactive compound from ethanolic extract of Euclea crispa (E. crispa) leaves. Column chromatography method was used to isolate the bioactive compound from the plant leaves followed by UV-Spectroscopy. FT-IR, 1H and 13C NMR analyses were used to identify the structure of the isolated compound. Subsequently, the antioxidant activity was demonstrated by 2, 2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging assay, reducing power assay, ferric reducing antioxidant power (FRAP) assay, hydroxyl scavenging assay and nitric oxide scavenging assay while disc-diffusion assay was used to analyze the antimicrobial activity. The NMR and other spectral data confirmed that the isolated compound was (6E, 10E)-2, 6, 24-trimethylpentacosa-2, 6, 10-triene (compound-1). The DPPH radical scavenging (116.1 ± 0.9 μg/ml), hydroxyl scavenging (118.9 ± 1.0 μg/ml) and nitric oxide scavenging (124.3 ± 0.2 μg/ml) showed the significant half maximal inhibitory concentration (IC50) values on compound-1 suggesting good antioxidative potential. Additionally, it exhibited considerable activity in reducing power and FRAP (the maximum absorption of 0.41 ± 0.02 and 0.54 ± 0.02 at 500 μg/ml, respectively) when compared with standard drug. Furthermore, compound-1 showed maximum antimicrobial activity with inhibition zone against the tested microorganism of Staphylococcus aureus, Streptococcus aureus, Escherichia coli, Klebsiella pneumonia, Aspergillus niger and Aspergillus terreus. Conclusively, the isolated bioactive compound-1 from E. crispa leaves could be a new source of natural antioxidative and antimicrobial agents with potential value.

Modified Magnetic Nanoparticle-Based Solid-Phase Extraction for the Determination of Trace Amounts of Aflatoxins B1 and B₂ in Chinese Patent Medicines: The Use of Fupuganmao Granules as a Case Study.

Background: Aflatoxins (AFs) have attracted increasing amounts of attention in recent years for their high toxicity. Previously, AF-polluted Chinese Patent Medicines (CPMs) were ignored, but their quality, safety, and efficacy might be influenced by the toxic compounds. Objective: The objective is to develop a simple, low-cost, and efficient method for quantitative analysis of aflatoxins B1 (AFB1) and B₂ (AFB₂) in CPMs to ensure their safe use. Methods: A simple, modified, magnetic nanoparticle-based solid-phase extraction (SPE) combined with HPLC-fluorescence detection for the determination of trace amounts of AFB1 and AFB₂ was established. The main parameters affecting the efficiency of modified magnetic nanoparticle-based SPE, such as pH of sample solution, adsorbent amount, adsorption time, and desorption condition, were investigated. Results: Under the optimum conditions, AFB1 and AFB₂ were linear in the ranges of 0.3-10 and 0.04-3 ng/mL with the correlation coefficient (R) of 0.9998 and 0.9999, respectively. Their intraday precisions were 1.16 and 2.30% and the interday precisions were 1.28 and 1.87% for AFB1 and AFB₂, respectively. The developed SPE was applied for AFB1 and AFB₂ extraction in three commercially available Fupuganmao granule samples, and the results were compared with the official immunoaffinity column chromatography method. Conclusions: The method provided a preferable candidate for the determination approach of AF measurement in CPMs. Highlights: Amine-functionalized magnetic nanoparticles were successfully applied to SPE for adsorbing AFB1 and AFB₂ in CPMs for the first time.

ISOLASI SENYAWA TRITERPENOID DAN UJI ANTIBAKTERI EKSTRAK n-HEKSAN DAUN KAYU ARA (Ficus aurata (Miq.) Miq)

Triterpenoid isolation from n-hexane extract of kayu ara leaves (Ficus aurata (Miq.) Miq) has been performed. The Isolation has been done by column chromatography method using silica gel as a stationary phase and n-hexane and ethyl acetate as mobile phase with isocratic method and it is continued by trituration method. Isolated compound has white amorphous shape, decomposed at 86-87ºC and gives single spot with reddish brown violet in LB reagent and in H2SO4 reagent on TLC test. UV spectra indicate the presence of double bonds in the isolated compound at 204 nm λmax and IR spectra showed -OH functional group at 3290 cm-1, -CH stretching at 2912,90 cm-1, C=C at 1676 cm-1, C-O stretching at 1054,93 cm-1, and at 1374 cm-1 is spectra of geminal dimethyl compound that are characteristic of triterpenoids which supports ultraviolet spectroscopic data. Antibacterial activity test showed that isolated compound is active in inhibiting the growth of gram-positive bacteria Staphyloccocus aureus and gram-negative bacteria Escherichia coli.

Phenolic constituents from Wikstroemia chamaedaphne

The phenolic constituents of Wikstroemia chamaedaphne were investigated by various column chromatographic methods including silica gel,Sephadex LH-20,ODS and preparative HPLC,and their chemical structures were identified by physico-chemical properties and spectral analyses. Thirteen phenolic compounds were isolated and elucidated,including five flavonoids: luteolin 7-O-β-D-glucopyranoside(1),luteolin 4'-O-β-D-glucopyranoside(2),kaempferol 3-O-β-D-glucopyranoside(3),chrysoeriol 4'-O-β-D-glucopyranoside(4),chrysoeriol(5); and eight lignans:(-)-secoisolariciresinol(6),acanthosessilin A(7),(-)-nortrachelogenin(8),(+)-isolariciresinol(9),sesamin(10),syringaresinol(11),(+)-epipinoresinol(12),and [3,3',4,4'-tetrahydro-6,6'-dimethoxy-3,3'-bi-2 H-benzopyran]-4,4'-diol(13). Compounds 1, 3, 5-8, 10, 11 and 13 were obtained from the plants of W. chamaedaphne for the first time,and compounds 1,5,7,10 and 13 were obtained from the Wikstroemia genus for the first time.

Antibacterial Activity of Acacia nilotica Stem-Bark Fractions against Staphylococcus aureus and Escherichia coli

Aims: This research was carried out to determine the antibacterial activity of Acacia nilotica stem bark extract and bioactive fractions against the test bacteria (Staphylococcus aureus and Escherichia coli).
 Place and Duration of Study: Acacia nilotica was collected within Aliero town, Kebbi State, Nigeria between April and September, 2017.
 Methodology: The crude and bioactive fractions were obtained using soxhlet extraction and column chromatographic method respectively. The qualitative phytochemical screening was conducted to detect the presence of some phytochemical constituents in the crude extract and fractions. The antibacterial activity was determined at various concentrations (10, 50, 100, 150 and 200 mg/ml) using disc diffusion method.
 Results: The crude antibacterial activity indicated that ethanol extract showed higher activity than the n-hexane extract with 14.0±0.00 and 12.0±0.00 mm zones of inhibition compared with the control drug (10 µg Ciprofloxacin drug), which showed 14.0±0.00 and 13.0±0.00 mm zone of inhibition against the test bacteria. The MIC and MBC values determined for ethanol extracts against the test bacteria was 12.5 mg/ml and 25 mg/ml, while the MIC and MBC values obtained for n-hexane extracts were 25 and 50 mg/ml against the test bacteria. The bioactive fractions (Yellow, Purple and Blue Black Fractions) tested against the test bacteria showed higher activity compared with the crude extract. The phytochemical properties of the plant crude extract and the bioactive fractions indicated the presence of phenol, tannins, alkaloids, saponins, flavonoids, terpenoids, steroids and glycosides and this attributed to the high antibacterial activities of 17.0±0.00 and 16.0±0.00 mm showed by the fractions against Staphylococcus aureus and 15.67± and 14.0±0.00 mm against Escherichia coli respectively.
 Conclusion: Acacia nilotica crude extract and fractions exhibited antibacterial activity which was comparable to the standard drug ciprofloxacin. This validates the folkloric medicinal use of this plant by the indigenous people of Aliero, Kebbi State.

Anti-neuroinflammatory activities of extract and polymethoxyflavonoids from immature fruit peels of Citrus 'Hebesu'.

Chronic neuroinflammation is reported to be associated in the progression of many neurodegenerative diseases and there is an increasing interest for the natural products as neuroprotective and anti-neuroinflammatory agents. In present research, we evaluated the potential inhibitory effects of extract, fractions, and isolated compounds from Citrus 'Hebesu' on lipopolysaccharide (LPS)-induced inflammatory responses using BV-2 murine microglial cells. The dried methanol extract (CH) was suspended in water and partitioned with hexane and ethyl acetate to give hexane soluble (CHH), ethyl acetate soluble (CHE), and water soluble (CHW) fractions. The extract (CH) and fractions CHH and CHE inhibited the expression of mRNA encoding pro-inflammatory cytokine interleukin (IL)-1β. CHE and CHH were further purified by various column chromatographic methods to obtain hesperidin (1), tangeretin (2), 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone (3), 3,5,6,7,8,3',4'-heptamethoxyflavone (4), nobiletin (5), 3,4,5-trimethoxy-trans-cinnamic alcohol (6), and meranzin hydrate (7). Among them, three polymethoxyflavonoids, 3, 4, and 5 significantly inhibited the expression of IL-1β mRNA. PRACTICAL APPLICATIONS: Citrus 'Hebesu' is a local cultivar in Hyuga City, Miyazaki prefecture, Japan and its immature fruits are consumed with different food recipes. Till now, there is no detailed study on the chemical constituents and anti-neuroinflammatory activity of this cultivar. In this study, seven compounds were isolated from the peels of immature fruits. Methanol extract, hexane, and ethyl acetate fractions and three polymethoxyflavonoids showed a significant inhibitory activity against expression of IL-1β mRNA. Consumption of peels of Citrus 'Hebesu' might play important role in the prevention and treatment of neurodegenerative diseases, however, detailed mechanism based in vivo studies are necessary in future for providing more scientific evidences.

Extraction and Isolation of Kaempferol Glycosides from the Leaves and Twigs of Lindera neesiana

The hydroalcoholic extract of leaves and twigs of Lindera neesiana (Wall. ex Nees) Kurz (Lauraceae) was subjected to various column chromatographic methods that isolated five kaempferol glycosides: kaempferol 3-O-β-glucopyranosyl(1→2)-[α-rhamnopyranosyl-(1→6)]-β- glucopyranoside-7-O-α-rhamnopyranoside (1); kaempferol 3-O-β-glucopyranosyl-(1→2)-[α-rhamnopyranosyl (1→6)]-β-glucopyranoside (2); kaempferol 3-O-β-glucopyranosyl(1→2)- α-rhamnopyranoside-7-O-α-rhamnopyranoside (3); kaempferol 3-O-sophoroside (4); and kaempferol 3-O-α-rhamnopyranoside (5). The extract showed moderate free radical scavenging activity and potent pancreatic lipase inhibitory activity.

Potential in vitro anti-allergic, anti-inflammatory and cytotoxic activities of ethanolic extract of Baliospermum montanum root, its major components and a validated HPLC method

BackgroundThe root of Baliospermum montanum has been used as an ingredient of traditional Thai medicines for the treatments of several diseases including itching eczema, muscle and joint inflammation, and cancer. Few studies have been done on phytochemical components of this root. In this study, we isolated major compounds of the crude ethanolic extract of B. montanum root and developed and validated a high performance liquid chromatographic (HPLC) method for the determination of its major components. We then investigated anti-allergic, anti-inflammatory and cytotoxic activities of the extract.MethodsThe aims of this study were to investigate in vitro activities including inhibitory effect of β-hexosaminidase released from RBL-2H3 cells, inhibition of nitric oxide (NO) production from RAW 264.7 cells and cytotoxic activity against cancerous liver cell lines (HepG2 and KKU M156) by using sulforhodamine B (SRB) assay. Isolation of major components was conducted by using column chromatographic method. Isolated major compounds were analyzed by using high performance liquid chromatography (HPLC).ResultsThe crude extract exhibited the highest cytotoxic activity, with IC50 less than 1 μg/mL, while its anti-allergy and anti-inflammation were also potent with IC50 less than 6 μg/mL. Three propiophenones isolated from B. montanum root exhibited moderate cytotoxic activities (IC50 > 20 μg/mL). Two of the propiophenones found were major components that can be detected by HPLC. The developed and validated HPLC method showed good accuracy, precision, and linearity.ConclusionThe results of this study suggested that ethanolic extract of of B.montanum root can be a potential source of anti-allergy, anti-inflammation, and anti-cancer compounds. The isolated compounds can serve as markers when B. montanum is used in herbal remedies but not as overall responsive markers. The HPLC method developed may be useful for quality control in the production of the extract and for further formulation developments. However, investigation of several associated biological activities is necessary before the development can proceed further. Minor active compounds should be isolated and a more sensitive analytical method should be developed to detail the key responsive components of the ethanolic extract of B. montanum root.

Chemical constituents from rhizome of Menispermum dauricum and their anti-hypoxic activities

To study the chemical constituents from the rhizome of Menispermum dauricum,fifteen compounds,N-methylcorydaldine( 1),thalifoline( 2),stepholidine( 3),acutumine( 4),daurisoline( 5),acutumidine( 6),dauricicoline( 7),bianfugecine( 8),6-O-demethylmenisporphine( 9),bianfugedine( 10),dauricoside( 11),eleutheroside D( 12),aristolactone( 13),aristoloterpenateⅠ( 14) and aristolochic acid( 15) were isolated from 75% ethanol extract of Menispermi Rhizoma by using thin layer chromatography and column chromatography methods. Their structures were identified based on their physicochemical properties and spectral data. Among them,compounds 12-15 were obtained from the genus Menispermum for the first time. Six alkaloids with higher contents were subjected to evaluate the anti-hypoxic activities by using MTT method. As a result,six alkaloids exhibited significant anti-hypoxia activities.

Study on chemical constituents from fruiting bodies of Ganoderma australe

Chemical constituents were isolated from the fruiting bodies of Ganoderma australe by various column chromatographic techniques and HPLC method, and their chemical structures were identified through the combined analysis of physicochemical properties and spectral data. Meanwhile, their α-glucosidase inhibitory activity and anti-oxidative ability were evaluated. Seven compounds were isolated from G. australe and were identified as 6-methoxyl-cyclo-(Phe-Ile)(1), applanoxidic acid A methyl ester(2), ergosta-7,22 E-dien-3β-ol(3), cinnamic acid(4), 5α,8α-epidioxy-(20S,22E,24R)-ergosta-6,22-diene-3β-ol(5), 1-(3, 4-dihydroxyphenyl) ethanone(6), salicylic acid(7) and benzoic acid(8). Among the compounds, compound 1 was a new cyclic dipeptide. Compound 2 was a new lanosta natural product, and compounds 4, 6, 7 and 8 were obtained from G. australe for the first time. Moreover, compounds 4 and 8 exhibited α-glucosidase inhibitory activity with inhibition rates of 36.8% and 34.7%, and compounds 4, 7 and 8 had a certain activity in DPPH free radical scavenging activity with IC_(50) values of 0.168, 0.458 and 0.170 g·L~(-1), respectively. The DPPH radical scavenging rate of compound 1 was 41.1%.

Features of the determination of amlodipine in a biological material

The purpose of the work is to determine the optimal conditions for isolating amlodipine, to purify it by the method of column chromatography and to develop a method for detecting it in biological material. TLC, GC-MS, low pressure column chromatography, and HPLC were used for analysis. We studied the comparative isolation of amlodipine from biological material using 13 isolating agents of organic nature, water, and aqueous solutions. The use of acetone as an insulating agent for the extraction of amlodipine from tissues of cadaver organs has been substantiated. The possibility of purification of the analyzed compound from the endogenous substances of the biomaterial is shown by the method of reversed phase chromatography in a column of the Silasorp S-18 sorbent of 30 μm. A technique has been developed for detecting amlodipine in the tissues of cadaveric organs (liver), which corresponds to the necessary parameters of linearity, selectivity, accuracy, precision and stability. The limits of detection and quantification of amlodipine by the proposed method are 0.25·10-6 and 4.0·10-6 g, respectively, in 1 g of the biomaterial.

Isolation of Scopoletin from Paederia foetida and its Antidiabetic Potential Using In silico Model

Event Abstract Back to Event Isolation of Scopoletin from Paederia foetida and its Antidiabetic Potential Using In silico Model Dai Chuan Tan1*, Ku Idayu Idris1, Nur Kartinee Kassim1*, Pei Cee Lim1, Intan Safinar Ismail1, Muhajir Hamid1 and Rou Chian Ng1 1 Putra Malaysia University, Malaysia Background Paederia foetida L. (Rubiaceae) is an edible plant widely distributed in India, Japan, Malaysia, and other Asian countries. This study investigates the enzyme inhibition and of P. foetida twig extracts obtained from two different locations namely Johor (PFJ) and Pahang (PFP) and its chemical constituents. Methods The isolation of phytoconstituents using column chromatography methods and in silico study of isolated compound with receptor (α-glucosidase and α-amylase) was carried out using molecular docking. Results The IC50 of α-glucosidase and α-amylase inhibition activity of the PFJ chloroform extract were 245.6 ± 0.01 and 9.595 ± 0.01 µg/mL, respectively. The IC50 of α-glucosidase and α-alpha amylase inhibition activity of PFP chloroform extract were 2457.2 ± 0.01 and 14.83 ± 0.05 µg/mL, respectively. Separation of PFP chloroform extract afforded ergost-5-en-3-ol (1), stigmasterol (2), γ-sitosterol (3) and scopoletin (4) meanwhile PFJ chloroform extract gave (2) and (4). Scopoletin was isolated for the first time from this species. In silico molecular interaction study between scopoletin and α-glucosidase and α-amylase showed that α-glucosidase-scopoletin inhibitor complex had better binding affinity with less energy value (-5.4 kcal/mol) than α-amylase-scopoletin inhibitor complex (-4.9 kcal/mol). Conclusion PFJ chloroform extract showed potent inhibitory activity toward α-glucosidase. The in silico assessment of scopoletin as antidiabetic agent indicated that α-glucosidase-scopoletin complex has good stability with binding affinity of -5.4 kcal/mol. P. foetida can be considered as potential resources for natural herbal antidiabetic drugs which can serve as alternative to synthetic oral hypoglycemic drugs with minimum side effects. Keywords: Paederia foetida, Scopoletin, alpha-Amylase, Alpha-glucosidase, molecular docking Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Oral Presentation Topic: Metabolic diseases Citation: Tan D, Idris K, Kassim N, Lim P, Ismail I, Hamid M and Ng R (2019). Isolation of Scopoletin from Paederia foetida and its Antidiabetic Potential Using In silico Model. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2019.63.00025 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Nov 2018; Published Online: 17 Jan 2019. * Correspondence: Mr. Dai Chuan Tan, Putra Malaysia University, Seri Kembangan, Malaysia, dctan@chhs.edu.my Dr. Nur Kartinee Kassim, Putra Malaysia University, Seri Kembangan, Malaysia, kartinee@upm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Dai Chuan Tan Ku Idayu Idris Nur Kartinee Kassim Pei Cee Lim Intan Safinar Ismail Muhajir Hamid Rou Chian Ng Google Dai Chuan Tan Ku Idayu Idris Nur Kartinee Kassim Pei Cee Lim Intan Safinar Ismail Muhajir Hamid Rou Chian Ng Google Scholar Dai Chuan Tan Ku Idayu Idris Nur Kartinee Kassim Pei Cee Lim Intan Safinar Ismail Muhajir Hamid Rou Chian Ng PubMed Dai Chuan Tan Ku Idayu Idris Nur Kartinee Kassim Pei Cee Lim Intan Safinar Ismail Muhajir Hamid Rou Chian Ng Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Sesquiterpene lactones in the aerial part of Artemisia maritima L.

Three substances are separated from the above-ground part of Artemisia maritima L. by column chromatography method in an individual state: C15H18O3 with a m.p. of 171-172°C (substance 1), C15H18O3 with a melting temperature of 130-131°C (substance 2), C15H18O4 with a melting point of 201- 202°С (substance 3), which are identified as α-santonin, glabellin and artemisin on the basis of 1H NMR spectra and direct comparison of IR spectra.

Progress in separation and purification of nattokinase and its enzyme activity determination

In this paper, the separation and purification of nattokinase have been reviewed by primarily focusing on solvent precipitation, adsorption column chromatography, magnetic microspheres, expanded bed, reverse micelle, and three-phase separation methods. The different methods for determination of the enzyme activity have been discussed and compared. Finally, the feasibility that the nucleic acid-based identification techniques can be used for nattokinase purification and enzyme activity assay has been proposed.

Separation Of Radioisotope 113mIn Using Column Chromatography Based on Silica Gel Matrix

Radioisotope indium-113m (113mIn) with half-life, T1/2 = 1.7 hours and gamma energy, Eg = 391 keV is suitable and meets the criteria as radiotracer in industry. 113mIn radioisotope was obtained from tin-113 decay (113Sn, T1 / 2 = 115 days) of 112Sn (n,g) 113Sn neutron activation in nuclear reactor. The process of separation of radioisotope 113mIn using column chromatography method based on silica gel matrix using 0.05 M HCI solution. Radionuclide and radiochemical purity tests were performed using the gamma-spectrometry method and paper chromatography. The final product specification in the form of 113mInCl3 is clear solution, pH 2, obtained yield of 81.83%, radionuclide purity of 90.22%, radiochemical purity of 91.61 ± 0,29% and stable for 3 days at room temperature.