Extraction and Isolation of Kaempferol Glycosides from the Leaves and Twigs of Lindera neesiana

Anjana Adhikari-Devkota,Amina Dirar,Kazuki Tsushiro,Hari Devkota,Ayumi Kurizaki

doi:10.3390/separations6010010

Anjana Adhikari-Devkota, Amina Dirar + Show 3 more

Open Access

https://doi.org/10.3390/separations6010010

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Journal: Separations	Publication Date: Feb 13, 2019
Citations: 9	License type: CC BY 4.0

Affiliation: Kumamoto University

Abstract

The hydroalcoholic extract of leaves and twigs of Lindera neesiana (Wall. ex Nees) Kurz (Lauraceae) was subjected to various column chromatographic methods that isolated five kaempferol glycosides: kaempferol 3-O-β-glucopyranosyl(1→2)-[α-rhamnopyranosyl-(1→6)]-β- glucopyranoside-7-O-α-rhamnopyranoside (1); kaempferol 3-O-β-glucopyranosyl-(1→2)-[α-rhamnopyranosyl (1→6)]-β-glucopyranoside (2); kaempferol 3-O-β-glucopyranosyl(1→2)- α-rhamnopyranoside-7-O-α-rhamnopyranoside (3); kaempferol 3-O-sophoroside (4); and kaempferol 3-O-α-rhamnopyranoside (5). The extract showed moderate free radical scavenging activity and potent pancreatic lipase inhibitory activity.

Highlights

The genus Lindera (Family: Lauraceae) consists of about 100 species widely distributed in tropical, subtropical, and temperate zones of Asia and Midwestern America [1]
In this study, we aimed for the isolation and identification of chemical constituents and the evaluation of free radical scavenging and pancreatic lipase inhibitory activities of the extract and compounds
Chemical shifts are given in ppm with reference to tetramethyl silane (TMS)

Summary

Introduction

The genus Lindera (Family: Lauraceae) consists of about 100 species widely distributed in tropical, subtropical, and temperate zones of Asia and Midwestern America [1]. Lindera plants and isolated compounds have been studied for different biological activities including cytotoxic [9,10], anti-inflammatory [11,12], anti-hypertensive [13], hepatoprotective [14], and antioxidant activities [15,16]. We isolated several flavonoids from the leaves and twigs of Lindera sericea, which is widely used as a herbal tea in Japan, and evaluated its free radical scavenging and enzyme inhibitory activities [20]. In this study, we aimed for the isolation and identification of chemical constituents and the evaluation of free radical scavenging and pancreatic lipase inhibitory activities of the extract and compounds

General Experimental Procedures

Plant Materials and Chemicals

Extraction and Isolation

Results

Conclusions