Colletotrichum Capsici Research Articles

Discovery of Acaricidal, Insecticidal, and Fungicidal Candidates Inspired by Natural Ethyl Cinnamate Compounds Isolated from Polygonum orientale L.

As a continuation of our research on the pesticide development of Polygonum orientale L., the chemical constituents of the seeds of P. orientale were systematically investigated. Eleven natural compounds (PO-1 to PO-11) were isolated from the EtOAc extract of P. orientale. Notably, compound PO-9 and its dimeric compound PO-10 were first isolated from P. orientale and possessed excellent acaricidal activity against Tetranychus cinnabarinus. With PO-9 and PO-10 as the lead compounds, two series of cinnamate derivatives were further synthesized, and their acaricidal, insecticidal, and fungicidal activities were evaluated systematically. The insecticidal activity results showed that dimeric derivative NKY-70 displayed the highest acaricidal activity against T. cinnabarinus and insecticidal activities against Brevicoryne brassicae and Myzus persicae. Furthermore, most of these compounds showed excellent in vitro antifungal activity against plant fungi. Compound NKY-66 displayed the highest and broad spectrum of antifungal activity against 23 fungi, and the respective EC50 values were 0.09, 0.08, 0.12, 0.18, 0.12, and 0.09 mg/mL against Valsa mali, Fusarium oxysporum f. sp. cucumerinum, Fusarium graminearum, Magnaporthe oryzae, Colletotrichum capsici, and Phytophthora infestans, which were more potent than those of chlorothalonil and procymidone. Moreover, the in vivo fungicidal evaluation also demonstrated that compound NKY-66 could effectively control plant fungal diseases in the greenhouse and in the field, such as damping off, powdery mildew, and cucumber downy mildew. Therefore, these findings implied that the cinnamate derivative NKY-66 displayed superior in vitro and in vivo fungicidal activities and could be a potential candidate against plant fungal diseases.

Endophytic fungi from <i>Dichrocephala integrifolia</i>: Diversity, antifungal properties, enzymatic activities, and plant growth promotion

Dichrocephala integrifolia is a wild medicinal plant utilised in traditional healing and Ayurveda to cope up with several health issues by various groups of people around the world. Medicinal plants are associated with diverse fungal endophytes with potential bioactive properties. In this investigation, 26 fungal endophytes were isolated from D. integrifolia and three sterile forms using the Petri plate culture method. The endophytic isolation rate was highest for inflorescence (35.97%) and lowest for stem (15.61%). The highest colonization frequency was shown by F. solani (8.37%) and the lowest by Sterile morphotype 3 (1.36%). Out of the four plant parts, inflorescence was found to be highly infected, displaying an infection rate of 93.43% and the least infection occurred in the stem with 45%. The maximum number of isolated endophytic fungi belongs to the class Sordariomycetes, with a relative occurrence (%) of 71.72%. The Simpson’s diversity index reveals that the leaf endophytes were more diverse (0.94). Qualitative antifungal activity of the sporulating isolates against Curvularia lunata has shown that the maximum number of endophytes possessed Class 3 antagonism. Four isolates were selected based on screening of their antagonistic activity and their antifungal inhibition was calculated against nine fungal phytopathogens. Maximum inhibition (100%) was shown by Trichoderma sp. 2 (S2B2) against Alternaria alternata, A. brassicicola, Colletotrichum capsici, C. lunata, and Ustilaginoidea virens and least inhibition by Gliocladium sp. 1 (19.78%) against C. lunata. The four isolates were found to produce protease, lipase, amylase and cellulase enzymes. The isolates produced ammonia and hydrogen cyanide, but none of the isolates could solubilize phosphate. Potent biocontrol agents are much needed to replace synthetic chemicals and restore soil microflora.

Invitro efficacy of fungicides against seed borne Colletotrichum capsici in chilli

Invitro efficacy of fungicides against seed borne Colletotrichum capsici in chilli

Corrigendum: Antifungal evaluation of turmeric rhizome extract against Colletotrichum capsici, the causal agent of anthracnose on red-chili peppers (Capsicum annuum L.)

Antifungal evaluation of turmeric rhizome extract against Colletotrichum capsici, the causal agent of anthracnose on red-chili peppers (Capsicum annuum L.) by Akin, HM, Aggraini D, Wibowo L, Prasetyo J & Suharjo R. 2024. J. Trop Plant Pests Dis. 24(1): 75–81. https://doi.org/10.23960/jhptt.12475-81 In the original article, on page 77, the formula for disease intensity has an incomplete legend. The authors apologize for this mistake and clarify that it does not affect the scientific conclusions of the article. The original version has been corrected

Potential Antagonists Trichoderma viride as Biofungicide, Plant Spacing, and Agricultural Lime Application to Suppress Anthracnose on Chili

Anthracnose caused by Colletotrichum capsici and C. gloesporium on chili is a disease that can reduce chili yields up to 80%. Control with fungicide has not been able to provide maximum results, because Colletotrichum can spread due to splashing of water, especially in the rainy season. The use of antagonistic fungi against Colletotrichum spp. has been widely published but is still limited to the laboratory and greenhouse scale, while field conditions are unpredictable. This study aims to identify the potency of Trichoderma viride that can be used as a biofungicide to control anthracnose in chili and to determine aspects of agronomic that can reduce the risk of anthracnose in chili. Samples of infected plants of stems, leaves, and fruits were collected from experimental farm of Bogor Agricultural Development Polytechnic. The experiment was conducted using Randomized Complete Design and Randomized Complete Block Design. The percentage of disease intensity of the fungus C capsici and the intensity of anthracnose in chili both under screen house and open fields. While testing the effect of cultivation aspects using different types of fertilizer on plant height, fruit weight, number of fruits, and the percentage of disease intensity of Colletotrichum spp. were analyzed. The results of this study shown that four isolates of fungi have been identified such as Penicillium sp., Aspergillus flavus, T. viride, and C. capsici. In vitro analysis shown the ability of T. viride to suppress the growth of the C. capsici up to 71%. The fungus T. viride with a density of 7×106 CFU/mL can suppress the development of anthracnose by 59 to 87% under screen house conditions. However, under field conditions, the fungus T. viride was not able to suppress the development of anthracnose. Agronomic aspects such as plant height, number of fruits and production, and productivity of chili were not significantly effect on anthracnose.

Potensi Cendawan Antagonis Trichoderma Viride Isolat Bogor sebagai Agensi Pengendalian Hayati Penyakit Antraknosa Tanaman Cabai Merah

Anthracnose disease caused by Colletottrichum capsici on chili plants that can damage yields by up to 80%. Control with fungicides has not been able to provide maximum results, because the fungus can move due to water splashes, especially in the rainy season. The use of antagonistic fungi against Colletotrichum capsici. has been widely published but is still limited to laboratory and greenhouse scales. This study aims to analyze the potential of Bogor isolate Trichoderma viride antagonist fungus against the fungus C. capsici through in-vitro study in the laboratory, screen houses, and field conditions. The experiment was structured using Complete Randomized Design (CRD) with parameters that measured respectively the antagonistic ability, and the percentage of attack by C. capsici and the severity of anthracnose disease on chili plants were both tested in screen house condition. Bogor Isolate T. viride has potential as a biological control agent for chili plants. This is proven by the ability to suppress the growth of C. capsici by 71% in vitro. The use of the fungus T. viride with a concentration of 7x106 conidia/ml (full strength) can suppress the development of anthracnose by 59 to 87% under screen house conditions. In other words, the pathogenicity of capsici in red chili plants shows a severity level of 13 to 41%.

Trichoderma-Mediated Synthesis of ZnONPs: Trend Efficient for Repressing Two Seed- and Soil-Borne Phytopathogens Phomopsis vexans and Colletotrichum capsici

Trichoderma-Mediated Synthesis of ZnONPs: Trend Efficient for Repressing Two Seed- and Soil-Borne Phytopathogens Phomopsis vexans and Colletotrichum capsici

Cultural, Morphological and Pathogenic Variability in the Colletotrichum capsici Isolates, Inciting Anthracnose and Fruit rot of Chilli (Capsicum annuum L.) in different Agro-Climatic Zones of Kerala, India

Aims: To study the cultural, morphological and pathogenic variability existing among the pathogen isolates of Colletotrichum capsici, inciting anthracnose and fruit rot of chilli in Kerala. Study Design: Completely randomized design (CRD). Place and Duration of Study: Department of Plant Pathology, College of Agriculture (Kerala Agricultural University), Vellayani, Thiruvananthapuram during 2018-2019. Methodology: A survey was conducted in the five agro-climatic zones (ACZs) of Kerala viz., Northern zone, Central zone, Special problem zone, High range zone and Southern zone to assess the incidence and severity of anthracnose disease. The various symptoms in the surveyed locations were also noted. Pathogen isolates were grown in PDA medium in the laboratory and colony characters (colour and mycelial growth rate of pathogen colonies) were observed. Also, the morphological characters viz., mycelial width, shape and size of conidia, acervular diameter, number and size of setae, appressorial size were recorded. Results: Maximum anthracnose incidence of 90 per cent and severity of 52.60 per cent were noticed at Regional Agricultural Research Station (RARS), Pilicode (Northern zone). Characteristic leaf spot, fruit rot and die-back symptoms were observed in the survey locations. Pathogen colonies showed different shades of white, off-white to grey turning brown or black with regular or irregular margins and concentric rings of black acervuli in the potato dextrose agar (PDA) medium. The diameter of growth of C. capsici isolates ranged from 7.20 to 8.60 cm at 7 days after incubation. All the C. capsici isolates initiated hyaline mycelium having a width of 1.73 - 2.36 µm and produced numerous sickle shaped conidia with a size range of 19.42 - 20.46 µm × 2.16 - 3.09 µm. Black coloured, circular or ellipsoidal acervuli (122.14 - 189.08 µm dia.) had 20 – 46 setae of length 74.13 to 107.30 µm. Also, brown or black appressoria of size 8.64 - 12.64 µm × 5.54 - 7.84 µm were formed in slide culture. Cc3 from College of Agriculture, Vellanikkara was obtained as the most virulent isolate producing a lesion size of 1.13 cm and PDI of 45.33 in the artificially inoculated chilli fruits (var. Vellayani Athulya) at 5 days after inoculation. Conclusion: All the nine C. capsici isolates exhibited variability in their cultural, morphological and pathogenic characteristics. Variability in phytopathogenic fungi helps in identifying novel and sustainable control methods to tackle the diseases incited by them. Understanding pathogen variability provides new insights for developing effective disease management methods. This will help in promoting sustainable agriculture and preventing severe crop losses.

Green synthesis of silver nanoparticles for their antifungal activity against anthracnose disease causing Colletotrichumcapsici

Anthracnose disease caused by Colletotrichum capsici, is a major fungal issue in chilies (Capsicum annuum L.) worldwide, leading to a reduction in global production. It can be controlled by using synthetic fungicides, but these fungicides can hamper the environmental and ecological balance. As a result, alternative approaches are needed to control this important fungal disease. In this study, economically feasible silver nanoparticles (AgNPs) were manufactured and demonstrated antifungal activity against anthracnose disease causing Colletotrichum capsici. AgNPs were synthesized from Colchicum luteum leaf extract. Physico-chemical characterization confirmed the synthesis, average size, and shape of AgNPs. The AgNPs were spherical in shape, with an average size of 11.75 nm. The isolated fungal pathogen causing anthracnose in chili was identified as C. capsici. The antifungal activity of AgNPs, AgNO3, and a fungicide was evaluated, with AgNPs showing the strongest inhibitory impact on the mycelial growth of C. capsici. In an in vitro leaflet assay, AgNPs demonstrated significant efficacy in inhibiting fungal proliferation on chili leaves. The results highlight the ability of AgNPs as antifungal agents against C. capsici, surpassing the effectiveness of both AgNO3 and conventional fungicide treatments. These findings advocate for continued investigation into the practical application of AgNPs as a promising alternative strategy for managing fungal diseases in agricultural settings.

Screening of Germplasms for Disease Resistance Against Anthracnose of Chilli Caused by Colletotrichum capsici

Chilli (Capsicum annuum Linn.) is an important extensively grown spice crop. It belongs to the family Solanaceae. Approximately 20-27 species of chilli are found, out of which five are domesticated viz., C. annuum, C. baccatum, C. chinense, C. frutescens, and C. pubescens in different parts of the world. Chilli crop suffers from various diseases in which anthracnose caused by Colletotrichum capsici is a very serious one, causes huge yield losses in India. Severely infected fruits look straw colour and bear numerous acervuli in concentric rings. Around 27℃ temperature, 80% relative humidity and soil pH 5-6 promote infection and disease progress. The average disease incidence level ranges between 66 and 84% which results in yield loss upto 12-50% This investigation was carried out at the Main Experiment Station Vegetable Science and Department of Plant Pathology, Acharya Narendra Deva University of Agriculture & Technology Kumarganj, Ayodhya (U.P) during the year 2021 -2022. Twenty genotypes of chilli were screened and none one genotypes are found resistant, nine genotypes namely, Mahycojalna, Arkalohit, Phulemukta, Arkaharita, Madhurima, Classica 152, Arkameghna, Sonakshi 44 and Divyajyoti were found moderately resistant and rest of the genotype were susceptible and highly susceptible.

Evaulation of Effective Antagonistic Yeasts in Kalasin Province, a Biocontrol Agent against the Fungal Colletotrichum capsici Caused Anthracnose Diseases in Chili Fruits

Primary screening of epiphytic and endophytic yeasts, the majority yeast was found from rhizosphere soil (21 isolates, 31.81%), and 5 antagonistic yeasts can have inhibited the growth of mycelia of C. capsici up to 60%. Efficacy of five antagonistic yeasts were screened under plant nurseries conditions. The result found all isolates were tested and compared with control and chemical plots. The result of plant height showed that, yeast isolate KS34 had highest of plant height at 45.80 cm. For the result of branch per plant of chili plant found isolate KS37 showed highest of branch per plant at 16.60 branches and the flower of chili per plant presented that isolate KS37 had highest of flower at 18.60 flowers. The result of anthracnose disease control by antagonistic yeasts in disease incidence and category mean as plant resistance. These results found that, Isolates KS46 had a great disease incidence at 7.80% that mean this isolate prove chili plant to resistance to C. capsisci. Addition, isolate KS46 had highest of activity of chitinase enzyme at 170.72 U/ml. Isolate KS46 identified by D1/D2 domain of 26S ribosomal DNA sequencing. The sequence analysis showed maximum identity of 100% with Candida haemulonii. This assumed C. haemulonii was showed the best to control C. capsici. For confirmed this result, all isolates were determined the mode of action by studied in relationship between antagonist isolates. The action of yeast isolate could competition the nutrient with mycelia of pathogen. This mode effect to C. capsici loss nutrient caused the pathogen get stuck to braked and died later.

Fungitoxicants and bio-formulations for the management of Chilli anthracnose caused by Colletotrichum capsici (Syd.) Butler & Bisby

Fungitoxicants and bio-formulations for the management of Chilli anthracnose caused by Colletotrichum capsici (Syd.) Butler & Bisby

Antifungal evaluation of turmeric rhizome extract against Colletotrichum capsici, the causal agent of anthracnose on red-chili peppers (Capsicum annum L.)

This study was conducted to evaluate the antifungal activity of the ethanolic extract of turmeric rhizome against Colletotrichum capsici Syd. and its efficacy in reducing anthracnose disease development on red chili peppers. The in vitro experiment aimed to determine the effect of the extract on inhibiting C. capsici growth in potato sucrose agar (PSA) medium incorporating the turmeric rhizome extract. The variables examined were mycelial growth, fungal sporulation, and spore germination. The in vivo experiment aimed to evaluate the effectiveness of the extract in reducing disease development on red chili peppers. The treatments were conducted using a completely randomized design with five concentration treatments and three replicates. The variables examined were radial mycelial growth zone diameter, fungal sporulation, spore germination, and disease intensity. Data were analyzed by analysis of variance, and the least significant difference was used for comparing means between treatments. The results of these experiments showed that ethanolic turmeric rhizome extract exhibited antifungal activity against C. capsici both in vitro and in vivo conditions. Turmeric rhizome extracts with concentrations of 1%, 2%, 3%, and 4% significantly inhibited mycelial growth, and even at a 4% concentration, C. capsici fungal colonies did not grow. Evaluation of the effect of the extract on fungal sporulation showed that inhibition occurred at a concentration of 3%, while inhibition of spore germination occurred at concentrations of 1%, 2%, and 3%. Application of turmeric extract at concentrations of 1%, 2%, 3%, and 4% effectively reduced the intensity of anthracnose on red chili.

Comparative Transcriptomics Analysis Reveals the Differences in Transcription between Resistant and Susceptible Pepper (Capsicum annuum L.) Varieties in Response to Anthracnose.

Pepper (Capsicum annuum L.) is a herbaceous plant species in the family Solanaceae. Capsicum anthracnose is caused by the genus Colletotrichum. spp., which decreases pepper production by about 50% each year due to anthracnose. In this study, we evaluated the resistance of red ripe fruits from 17 pepper varieties against anthracnose fungus Colletotrichum capsici. We assessed the size of the lesion diameter and conducted significance analysis to identify the resistant variety of B158 and susceptible variety of B161. We selected a resistant cultivar B158 and a susceptible cultivar B161 of pepper and used a transcription to investigate the molecular mechanisms underlying the plant's resistance to C. capsici, of which little is known. The inoculated fruit from these two varieties were used for the comparative transcription analysis, which revealed the anthracnose-induced differential transcription in the resistant and susceptible pepper samples. In the environment of an anthrax infection, we found that there were more differentially expressed genes in resistant varieties compared to susceptible varieties. Moreover, the response to stimulus and stress ability was stronger in the KANG. The transcription analysis revealed the activation of plant hormone signaling pathways, phenylpropanoid synthesis, and metabolic processes in the defense response of peppers against anthracnose. In addition, ARR-B, AP2-EREBP, bHLH, WRKY, and NAC are associated with disease resistance to anthracnose. Notably, WRKY and NAC were found to have a potentially positive regulatory role in the defense response against anthracnose. These findings contribute to a more comprehensive understanding of the resistance mechanisms of red pepper fruit to anthracnose infection, providing valuable molecular insights for further research on the resistance mechanisms and genetic regulations during this developmental stage of pepper.

Influence of Different Culture Media on Growth and Sporulation of Colletotrichum capsici

Aim: To know the best culture media for growth and development of Colletotrichum capsici causing chilli anthracnose.
 Study Design: Twelve different culture media were selected and three replications were maintained.
 Place and Duration of Study: Department of Plant Pathology, University of Agricultural Sciences, Raichur, Karnatka, which was carried out in the year 2021-2023.
 Methodology: The laboratory study was conducted to know the best nutrient media for the growth and sporulation of the pathogen. The pathogen was isolated from infected chilli fruit using tissue isolation technique and pure culture was maintained. Twelve different nutrient media were selected and tested for the growth and sporulation of the pathogen in three replications.
 Results: Among twelve media tested, Red chilli dextrose agar have promoted the maximum growth of C. capsici (90.0 mm), which was followed by carrot dextrose agar and potato carrot agar (89.50 mm). Green chilli extract agar significantly promoted minimum growth of C. capsici (33.50 mm), which was followed by red chilli extract agar (48.00 mm) and Sarbouraud’s agar (48.50 mm). The cultural characters were varied among the media.
 Conclusion: Potato dextrose agar and carrot dextrose agar media were proved to be best media for growth and sporulation of Colletotrichum capsica.

Conidia deposition in liquid culture of Trichoderma using starch flour and antifungal activity of the precipitate against Colletotrichum capsici

Colletotrichum capsici is a highly destructive anthracnose pathogen, causing significant losses in chili plants. Trichoderma, an antagonistic fungus commonly mass-cultured, is employed to control anthracnose disease. This research aims to evaluate the efficacy of adding starch for conidia deposition in liquid Trichoderma cultures and to assess the antifungal activity of the sediment on the colony growth of C. capsici. The study utilized a factorial experiment with three types of liquid culture medium (5% tannin, 5% yeast, and 5% yeast + 5% tannin), three types of starch (rice, corn, and tapioca), and three concentrations (5%, 10%, and 20%), each with five repetitions and one control. Liquid culture, composed of a mixture of 2% sucrose with either yeast or tannin from the gambir plant (Uncaria gambir), was inoculated with a blend of three Trichoderma isolates and incubated for 14 days at 150 rpm. Starch was added according to the treatment and then centrifuged at 3000 rpm. The results indicated a significant 8.9–17.8 times log10 increase in conidia concentration after the settling treatment. The type of liquid culture medium and starch concentration significantly influenced the conidia concentration after deposition. The yeast liquid culture exhibited the highest conidia density at 4.96×1010/mL. The starch concentration that produced the highest conidia density after settling was 20%, yielding 4.51×1010/mL. The type of starch did not significantly influence the conidia concentration in the sediment. The methanol extract of sedimented liquid culture, after the addition of 5-20% rice flour from tannin medium, exhibited antifungal activity against C. capsici.

Bioactive metabolites of licorice and thyme as potential inhibitors of Cox1 enzyme of phytopathogens of Capsicum annuum L.: In-silico approaches

Cytochrome c oxidase subunit 1 (Cox1), a key enzyme, has a crucial role in cellular respiration in eukaryotes and prokaryotes. Generally, respiratory inhibitors are considered one of the types of chemical pesticides. Thyme oil and licorice aqueous extract have been reported to have antifungal activities against fungal phytopathogens of Capsicum annuum L., i.e., Colletotrichum capsici, Fusarium oxysporum, and Pythium aphanidermatum. The present study focuses on identifying the key bioactive molecules of thyme and licorice botanicals inhibiting the activity of the Cox1 enzymes of the above mentioned phytopathogens, employing the in-silico approach. From a wide range of bioactive molecules screened, the molecular docking indicated trans-carveol, carvacrol, kaempferol 3-rhamnoside 7-xyloside, kaempferitrin, and astragalin 7-rhamnoside as the potential inhibitors for Cox1 of C. capsici, β-Caryophyllene, Caryophyllene acetate, hispaglabridin A, kaempferol 3-rhamnoside 7-xyloside and licorice glycoside A for Cox1 of F. oxysporum and (+)-Longifolen, Caryophyllene acetate, Hispaglabridin A, Neoliquiritin 2''-apioside and Licorice-saponin A3 for Cox1 of P. aphanidermatum. Most of the top-scoring bioactive molecules exhibited higher binding affinity with the targets than the chemical compound, i.e., carbendazim. Density functional theory (DFT) analysis confirmed the reactivity of the top-docked compounds. Molecular dynamic simulations confirmed the stability of docked complexes when evaluated through multiple descriptors. Additionally, MM/PBSA analysis supported the findings, indicating the spontaneous binding of the enzymes to the screened ligands. ADMET analysis revealed the safety of the selected bioactive compounds. The present findings could be useful in developing biopesticidal formulations as efficient and sustainable alternatives to chemical pesticides. Communicated by Ramaswamy H. Sarma

Test of the Effectiveness of Senduduk Leaf Extract Concentration (Melastoma Malabrhaticum L.) Against Anthracnose Disease in Red Chili Commodities (Capsicum annuum L.)

The disease that causes low chili productivity in Indonesia is anthracnose, anthracnose disease caused by the fungus Colletotrichum spp. Efforts to control anthracnose disease are currently still being made using many synthetic pesticides. The use of synthetic pesticides can have a negative impact on the environment. Vegetable functions can be used as another effort to control anthracnose. Plant extracts can be used in anthracnose disease control are Senduduk leaf extracts. This study aims to obtain the concentration of Senduduk plant leaf extract (Melastoma malabathricum L) in inhibiting the growth of Colletotrichum spp. Research is carried out in the field and laboratory. The design used in this research was a complete randomized design (RAL) with 5 treatments of Senduduk leaf extract, namely K0 = 0%, K1 = 3%, K2 = 6%, K3 = 9%, and K4 = 12%. With 3 repetitions. The results showed that Senduduk leaf extract treatment with a concentration of 12% effective could inhibit the growth of Colletotrichum capsici fungus.

Study on integrated disease managment of Anthracnose in chilli (Capsicum annuum L.) caused by Colletotrichum capsici (Syd.)

Study on integrated disease managment of Anthracnose in chilli (Capsicum annuum L.) caused by Colletotrichum capsici (Syd.)

Inventory of Chili Pests and Diseases in Kabandungan Sub-District, Sukabumi Regency

The decline in chili commodity production is influenced by various factors, including climate anomalies, less than optimal cultivation techniques, decreasing land quality, and pest attacks. Kabandungan District, as one of the chili production centers, can be used as a reference for studying the presence and intensity of chili pest attacks in general. Information about pest attacks can be used as a basis for control that can be applied in any location with similar characteristics. This research was conducted in Kabandungan District, Sukabumi Regency, West Java Province, Indonesia. This research was carried out from November 2021 to March 2022. The number of chili plants observed was 930 plants which were divided into 61 observation points spread across 6 villages, including Cianaga, Cihamerang, Cipeuteuy, Kabandungan, Mekarjaya, and Tugubandung Villages. The chili plants observed consisted of the local variety of cayenne pepper (CMR), cayenne red chili (CMR), curly red chili (CMK) variety TM 999, Darmais F1 large red chili (CMB), curly red chili (CMK), and chili curly red (CMK) OR Twist 42. The results of the study stated that the incidence of pest attacks was dominated by S. Litura attacks 26.40%, and Colletotrichum capsici 25.60% in the vegetative phase, as well as S. Litura 27.21%, and Gemini Virus 47.35% in the generative phase. S. litura attacks are reported to dominate all villages in Kabandungan District, while Mekarjaya Village is dominated by mites.