Coleus Blumei Research Articles

ANALISIS KADAR KUERSETIN PADA EKSTRAK ETANOL DAUN MIANA (Plectranthus scutellarioides (L.) R.Br.) SECARA HPLC (High Performance Liquid Chromatography)

One of the medicinal plants that people often use as a medicine is the Miana leaves (Plectranthus scutellarioides (L.) R.Br.). The Miana leaves are empirically used to treat coughs, heart disease, worm medicines, antimicrobials, antioxidants, antiseptics, bronchitis, hemorrhoids, tuberculosis, and other pharmacological activities. The miana leaves contain secondary metabolite compounds of flavonoids, steroids, tannins, and saponins. One of the most critical flavonoids is quercetin. This study aimed to determine the quercetin content of ethanol extract of miana leaves by HPLC (High Performance Liquid Cromatography). The extraction method by maceration using 96% ethanol. Determination of quercetin content in miana leaf ethanol extract by HPLC with C 18 column, maximum wavelength of 369.11 nm with mobile phase methanol : aquabides (59:41) flow rate of 1 mL/minute. The results showed retention time for quercetin as a comparison of 4,183 minutes, a sample of 4.563 minutes, and an additional sample of 4,363 minutes. The average content of quercetin in miana leaf ethanol extract was 3.122 mg/g or 0,312%.

Selenium-induced improvements in the ornamental value and salt stress resistance of Plectranthus scutellarioides (L.) R. Br.

Abstract Ornamental plants growing in urban areas are often exposed to salt stress that negatively affects their decorative value. Enhancement of their salt resistance to retain a high decorative value despite salt stress has therefore high practical importance. In our study, the exposure of Plectranthus scutellarioides (L.) R. Br. to NaCl-induced moderate (40 mM) or severe salt stress (80 mM) resulted in a number of stress responses including reduced growth parameters, decreased concentration of photosynthetic pigments, and an increase in the accumulation of anthocyanins and free proline. These changes were partially compensated for by the application of selenium (5 µM as Na2SeO4) to the growth medium. However, the beneficial effect of selenium on the growth and physiological parameters of P. scutellarioides was manifested only at the moderate level of salt stress. Under the severe salt stress, the application of selenium was not able to alleviate the phytotoxic effect of NaCl.

Photosynthetic activity of red and green leaf sectors in Coleus blumei plants as sensed by chlorophyll fluorescence

The changes and responses in the chlorophyll (Chl) fluorescence (ChlF) components were studied on the red and green parts of leaves in three Coleus blumei varieties under different light intensities. Chl a, carotenoids (Car), and SPAD were linearly, positively, and significantly correlated with total Chl [i.e., Chl (a+b)] of red and green leaves. Significant differences in Chl and Car contents were noted between differentially pigmented sectors of the leaf, but no anthocyanin content was found in the green parts compared to red leaves. Values of minimal and maximal fluorescence yields in dark- and light-adapted states in both red and green leaves were all positively and significantly correlated with Chl contents under dark conditions and all PPFD treatments for 20 min. Assessing the ChlF parameters under PPFD variations is an important tool for finding improved photosynthetic productivity of plants.

Effects of Light Quality on Vegetative Cutting and In Vitro Propagation of Coleus (Plectranthus scutellarioides)

Coleus (Plectranthus scutellarioides) is an attractive and popular ornamental plant with propagation mainly achieved through vegetative cuttings. For commercial purposes, it is of interest to enhance the speed of establishment while maintaining high quality. Light quality has been shown to influence adventitious root development, so these experiments examined the effect of narrow-bandwidth light treatments on root growth and overall plant quality for seven coleus cultivars with vegetative cuttings in potting soil and one cultivar with shoot tip in vitro cultures onto Murashige and Skoog (MS) agar medium. During the 28 days of the propagation period, the cuttings grown under narrow-bandwidth red light (R; 663.4 nm at peak) more than doubled in the adventitious root number compared with those under blue light (B; 445.7 nm at peak) and green light (G; 530.0 nm at peak) in five cultivars. R light also increased fresh weight of the cuttings by 55.6% more than G light. In comparison, the cuttings grown under G light yielded significantly lower root and shoot dry mass than other light treatments. R light cuttings showed more dry mass content (9.63%) than those under white light (W; 437.4 nm and 559.5 nm at peak) and G light (7.85% and 5.86%, respectively). A positive correlation (R2 = 0.598, P < 0.001) was found between the formation of adventitious roots and gained fresh weight of cuttings. R light made the reddish color of leaves significantly stronger in most cultivars, whereas the cuttings exposed to G light became less vivid compared with other light conditions. When the shoot tips were propagated in vitro onto MS medium, R light treatment initiated the root development more rapidly than other lights, with significantly greater rooting rate (20.0% and 63.6%, respectively) at day 5 and 10. The shoot tips under R light also formed significantly more roots (12.3 per cutting) than those grown under narrow-bandwidth B light (5.8 per cutting). The shoot tips showed browning at an early stage and newly emerged leaves grew very compactly under B light. The combination of red and green light (R+G) increased more than twice as much roots and dry mass compared with W light. In addition, the R+G light led to morphological changes, including larger leaves and longer petioles and internodes than those in other light treatments. The exposure to R+G+B and B light made the shoots very compact for the 28 days of in vitro culture period and significantly increased the chlorophyll contents resulting in dark green leaves.

Growth, Quality, and Economic Value Responses of Bedding Plants to Reduced Water Usage

This study analyzed the effects of two ranges of drying down of substrate moisture content (SMC) before re-watering on plant growth and development, postproduction quality, and economic value of bedding plants grown in 1.67-L containers during greenhouse production. The two SMC treatments were wide-range (WR) SMC (WR-SMC) for dry-down from container capacity (CC) of 54% SMC dried down to 20% SMC or narrow-range (NR) SMC (NR-SMC) for dry-down from CC of 54% SMC dried down to 40% SMC. Six bedding plant cultivars were used [Solenostemon scutellarioides ‘French Quarter’ (coleus); Petunia ×hybrida ‘Colorworks Pink Radiance’ (petunia); Lantana camara ‘Lucky Flame’ (lantana); Impatiens ×hybrida ‘Sunpatiens Compact Hot Coral’ (SCC); ‘Sunpatiens Spreading Lavender’ (SSL) (impatiens); and Salvia splendens ‘Red Hot Sally II’ (salvia)]. Shoot dry weight was reduced with WR-SMC on petunia, lantana, impatiens SCC, and salvia at the end of production. With WR-SMC, the petunia and impatiens SCC root ball coverage percentages were greater on the bottom of the container, whereas those of impatiens SSL and salvia were reduced. The WR-SMC increased petunia postproduction quality by increasing the flower number. Lantana and impatiens SCC inflorescence/flower and/or bud number were reduced with WR-SMC. The impatiens SSL flower number was unaffected by SMC treatment. Salvia grown with WR-SMC had increased postproduction quality. WR-SMC reduced postproduction water potential in petunia, lantana, and coleus, suggesting that plants with WR-SMC during production were acclimated to reduced irrigation administered during postproduction. WR-SMC saved labor due to less frequent watering and overhead-associated costs due to reduced bench space, with the exception of coleus and impatiens SSL, which used the same bench space as NR-SMC. Considering production and/or postproduction quality, using WR-SMC during greenhouse production is beneficial as an irrigation method for coleus, petunia, impatiens SSL, and salvia, but not for impatiens SCC or lantana grown in 1.67-L containers.

PROFIL GC-MS DAN NILAI TOKSISITAS LC50 FRAKSI NON POLAR DAUN Solenostemon scutellarioides (L) Codd.

ABSTRACT A study has been conducted to determine the chemical content profile of LC50 values from the hexane fraction of ethanolic extract of piladang leaves (Solenostemon scutellarioides (L.) Codd). The chemical content of the hexane fraction was analyzed by using GC-MS while the LC50 value was determined by the Brine Shrimp Lethality Test method. The results of the LC50 value of the hexane fraction of piladang leaf (Solenostemon scutellarioides (L.) Codd) was obtained 96.51 ppm included in the toxic category. GC-MS analysis results showed 25 different compounds of different area and composed of weak base compounds, esters, ketones, fatty acids and hydrocarbons. The main compound based on GC-MS data is 9-octadecenamide.Kata Kunci : Solenostemon scutellarioides, GC-MS, LC50, Brine Shrimp Lethality Test

Diversity, Ethnobotanical Potential and Sustainability Assessment of Plants Used by Traditional Healers to Treat Cancer in Boyo Division, North-West Region, Cameroon

Aims: Cancer is the second leading cause of death globally. Therefore, the knowledge on medicinal plants used to cure human cancer could be of great importance for their widespread use and scientific validation. The present study records information on anticancer plants in Boyo Division, in the western highland of Cameroon.
 Methods: Thirty traditional healers, were interviewed to document their know-how on the type of human cancer allegedly cured, the plant species used as well as their use pattern. Guided field walks were made to the collection sites for plant and its habitat characterization as well as herbarium voucher collection. Ethnobotanical quantitative tools were used to analyze and summarize collected data. Sustainability of harvest was assessed using a vulnerability index based on seven parameters. 
 Results: A total of 25 medicinal plants cited were identified as belonging to 13 families and 23 genera. The most represented families were Asteraceae (28%), Lamiaceae (16%), Fabaceae (12%) and Acanthaceae (8%). Out of the seven categories of cancer diseases reported, the highest number of plants species were reported to treat stomach, pancreas, liver, skin and breast cancers, with informant consensus factor (ICF) ranging from 0.79 to 0.82. Leaves (60%) and bark (20%) were the major plant parts used mostly in form of decoction (45.45%) and concoction (38.18%). The result of Relative frequency citations (RFCs) revealed that 9 of the 25 plants species cited were the most frequently used with fidelity levels ranging from 92% (Geniosporum rotundifolium and Ocimum tenuiflorum Aframomum melegueta and Entada abyssinica) to 100% (Coleus blumei, Ocimum gratissimum, Eremomastax speciosa, and Dichrocephala integrifolia). Six species were assessed as vulnerable (Vi ≥ 2), while two species were rated as highly vulnerable namely G. rotundifolium (Vi = 2.71) and E. abyssinica (Vi = 2.85).
 Conclusion: New traditionally effective anticancer plants were identified in the present study, some of which were already vulnerable for exploitation in their actual habitat. Plants with high ICF, RFCs and FL values should be subjected to further phytochemical and pharmacological investigations for scientific validation while those with high Vulnerability index should be recommended for participatory domestication by the main users.

Study on Micropropagation of Coleus Blumei Benth. through Apical Meristem Culture

Study on Micropropagation of Coleus Blumei Benth. through Apical Meristem Culture

Effectiveness of Mayana Leaves (Solenostemon Scutellarioides (L)) on Physical and Organoleptic Tests of Pork

Effectiveness of Mayana Leaves (Solenostemon Scutellarioides (L)) on Physical and Organoleptic Tests of Pork

Occurrence of Coleus blumei Viroid 6 in Commercial Coleus blumei in Canada: The First Report Outside of China

Occurrence of Coleus blumei Viroid 6 in Commercial <i>Coleus blumei</i> in Canada: The First Report Outside of China

Green Ultrasound Assisted Extraction of trans Rosmarinic Acid from Plectranthus scutellarioides (L.) R.Br. Leaves.

Painted nettle (Plectranthus scutellarioides (L.) R.Br.) is an ornamental plant belonging to Lamiaceae family, native of Asia. Its leaves constitute one of the richest sources of trans-rosmarinic acid, a well-known antioxidant and antimicrobial phenolic compound. These biological activities attract interest from the cosmetic industry and the demand for the development of green sustainable extraction processes. Here, we report on the optimization and validation of an ultrasound-assisted extraction (USAE) method using ethanol as solvent. Following preliminary single factor experiments, the identified limiting extraction parameters (i.e., ultrasound frequency, extraction duration, and ethanol concentration) were further optimized using a full factorial design approach. The method was then validated following the recommendations of the association of analytical communities (AOAC) to ensure the precision and accuracy of the method used to quantify trans-rosmarinic acid. Highest trans-rosmarinic acid content was obtained using pure ethanol as extraction solvent following a 45-minute extraction in an ultrasound bath operating at an ultrasound frequency of 30 kHz. The antioxidant (in vitro radical scavenging activity) and antimicrobial (directed toward Staphylococcus aureus ACTT6538) activities were significantly correlated with the trans-rosmarinic acid concentration of the extract evidencing that these key biological activities were retained following the extraction using this validated method. Under these conditions, 110.8 mg/g DW of trans-rosmarinic acid were extracted from lyophilized P. scutellarioides leaves as starting material evidencing the great potential of this renewable material for cosmetic applications. Comparison to other classical extraction methods evidenced a clear benefit of the present USAE method both in terms of yield and extraction duration.

Preferences and emotion perceptions of ornamental plant species for green space designing among urban park users in Iran

The main objective of the present study was to explore preferences and emotion perceptions of people in Rasht and Ardabil (Iran) to some ornamental plants in order to help designing appropriate urban green landscapes. The study surveyed urban park users in the two cities in 2017 to identify and compare the most important factors underpinning the viability of public open spaces from the perspective of users (mental aspect) and the extent to which these mental factors match the visual features of the selected spaces (visual aspect). A random sample of 232 individuals was taken from students in Ardabil (119 persons) and Rasht (113 persons). A considerable part of the participants (37.2%) reported a feeling of tranquility when they saw flowers, while 28.8% of the participants reported a feeling of love. Flower-bearing plant species were more appealing to the participants than leafy ornamental plants. Red was the most preferred flower color (52.1%) followed by violet (18.6%) and orange (11.4%) among the ornamental species commonly used in the design of green spaces, while tulip (Tulipa gesneriana) (29%), rose (Rosa hybrida) (25%), and Easter lily (Lilium longiflorum) (19%) were in the first top ranks of preferences, respectively. Coleus (Solenostemon scutellarioides) and garden croton (Codiaeum variegatum) were also reported among preferred species. The results support the assumption of similarity in preferences and requirements of citizens of large cities and despite great diversity in geography, culture, and ethics, people in large cities exhibit similar emotion perceptions. The study findings are expected to support public authorities and urban planners to effectively design and manage urban green spaces to meet users' needs.

Effects of feeding tyrosine or phenylalanine on the accumulation of polyphenols in Coleus Blumei in Vivo and in Vitro .

Background: Polyphenols are considered an important compound with a wide range of medical activities. Several plant families are rich with phenolics. Attempts have been carried out to increase them at the intact and cellular levels.&#x0D; Objective: The current study aimed to increase the production of polyphenols in Coleus blumei plant using two precursors at the whole and tissue culture levels. &#x0D; Materials and methods: Two precursors namely Tyrosine and phenylalanine at the concentrations 0.20 , 0.25 or 0.30 g.l-1 were added either by spraying on the vegetative parts or supplemented to the tissue culture medium. Total phenols were estimated in the whole plant and compared with the total phenolic content in callus tissues. They were estimated by Folin-ciocalteu and spectrophotometric methods. Induction and maintenance of Coleus callus cultures were carried out on Gamborg medium (B5) for 21 days supplemented with the growth regulators 2,4 dichlorophenoxy acetic acid (2-4-D) at 0.8 mg.l-1 , 2 mg.l-1 Benzyl adenine (BA) and 0.5 mg.l-1 Naphthalene acetic acid (NAA). Stem explants were used as a source for callus induction. &#x0D; Results: Results showed a significant increase in both fresh and dry weights of the fresh compared with control of intact plants in vivo after treatment with the two precursors. The fresh weight of shoots increased up to 15.9 g at 0.30 g.l-1 of Tyrosine treatment compared with the control which recorded 11.05 g. while the fresh weight of the shoots recorded 13.470 g at 0.30 g.l-1 after treatment with phenylalanine compared with the control which recorded 11.05 g. The dry weight of the shoots increased as well up to 3.127 g at 0.30 g.l-1 of Tyrosine treatment compared with control 1.837 g while the dry weight of shoot recorded 2.880 g at 0.30 g.l-1 phenylalanine treatment compared with the control 1.837g. Despite the slight increase in both root fresh and dry weights at 0.30 g.l-1 due to the treatment with Tyrosine or phenylalanine ,such increments were not significantly different with control. Total poly phenols in callus cultures accumulated more compared with mother plants after treatment with precursors. Phenols were also measured in both callus tissue and liquid medium then compared with the whole plant and control treatment. The highest value was recorded in callus cultures supplied with Tyrosine recording 348.36 µg.l-1 at 0.20 g.l-1 of Tyrosine while phenylalanine treatment recorded the highest weight 293.98 µg.l-1 when applied at 0.30 g.l-1 in both liquid medium and callus tissues. The intact plants however recorded the highest mean in total phenols in plants grown in vivo with mean values 68.58, 66.53 µg.l-1 for Tyrosine and phenylalanine respectively.&#x0D; Conclusion: It is concluded that both precursors are good candidates for increasing total phenols in Coleus blumei. Plant tissue culture techniques can be utilized commercially for this purpose.&#x0D;

Reação de espécies vegetais a Meloidogyne enterolobii e eficiência de seus extratos aquosos no controle do patógeno

The genus Meloidogyne includes species of the most common nematodes to affect crops around the world. The species M. enterolobii is notable for affecting and causing serious losses in the production of guava trees and various other economically important crops in Brazil. The aim of this study was to evaluate the susceptibility of 10 plant species to parasitism by M. enterolobii, and the in vitro and in vivo effects of their leaf extracts on the pathogen. Initially seedlings of Solenostemon scutellarioides, Dieffenbachia amoena, Spigelia anthelmia, Plumbago scandens, Ricinus communis, Chenopodium ambrosioides, Azadirachta indica, Morinda citrifolia, Jatropha curcas and Datura stramonium were inoculated with 5,000 eggs of M. enterolobii to evaluate their susceptibility to nematode infection. For the test in vitro, a 5% concentration of the leaf extracts were added to Petri dishes, and 50 J2 of M. enterolobii were placed in each dish. After 48 hours incubation, the juveniles were evaluated for motility and mortality in the extracts. For the test in vivo, leaf extracts were used at the same concentration, however with only the seven most-promising in vitro species. This assay included the following sequence: inoculation of 5,000 eggs in autoclaved and moist soil contained in 1L pots; application of 30 ml of extract to the soil after 24 hours; transplanting of ‘Santa Clara’ tomato seedlings the following day; and reapplying the extract after 7 and 14 days. The results were evaluated 45 days after nematode inoculation. It was seen that the species D. amoena, R. communis, A. indica, M. citrifolia, J. curcas and D. stramonium displayed highly resistant behaviour; S. anthelmia, P. scandens and C. ambrosioides were very resistant, whereas S. scutelarioides was susceptible to the nematode. With the in vitro test, extracts from seven of the 10 species caused 70.4% to 97.4% J2 mortality. Applying the best leaf extracts to the soil was efficient in reducing M. enterolobii infestation in roots of the tomato.

Effects of Growing Medium Type and Predator:Prey Ratio on Rove Beetle, Dalotia coriaria (Coleoptera: Staphylinidae), Adult Predation on Fungus Gnat, Bradysia sp. nr. coprophila (Diptera: Sciaridae), Larvae

Fungus gnats, Bradysia spp. (Diptera: Sciaridae), are major insect pests because the larvae can directly damage plants grown in greenhouse production systems. In general, insecticides are commonly used to suppress fungus gnat larval populations. However, the rove beetle, Dalotia coriaria (Kraatz) (Coleoptera: Staphylinidae), a commercially available predator of insect pests, including fungus gnat larvae, may be an alternative to using insecticides. Growing medium selection used for growing plants can influence insect pest and predator interactions in greenhouse production systems; however, quantitative information is limited. Therefore, we conducted greenhouse experiments and a laboratory experiment to determine the effects of growing medium type on D. coriaria adult predation and fungus gnat, Bradysia. sp. nr. coprophila (Lintner) (Diptera: Sciaridae), larval survival when feeding on the roots of coleus, Solenostemon scutellarioides (L.) Codd, plants. In addition, experiments were conducted to determine the number of rove beetle adults, based on predator:prey ratios, needed to regulate fungus gnat larval populations. In all three experiments, fewer fungus gnat larvae survived in Pro-Mix BX than the Berger BM1 growing medium, and rove beetles reduced the number of fungus gnat larvae in both growing media in the greenhouse experiments. Although predator:prey ratio was not significant, we found that 10 rove beetle adults per 15.2-cm diameter container (1834.82 cm3 with 2.0 L of growing medium) regulated fungus gnat larval populations at the densities investigated. Therefore, greenhouse producers should consider the effects of production practices, such as growing medium type, when using rove beetles to regulate fungus gnat larval populations.

New diterpenoid glucoside and flavonoids from Plectranthus scutellarioides (L.) R. Br.

Three new compounds, diterpenoid glucoside (13S,15S)-6β,7α,12α-trihydroxy-13β,16-cyclo-8-abietene-11,14-dione 7-O-β-D-glucoside 1, flavonoids apigenin 7-O-(3′′-O-acetyl)-β-D-glucuronide 2 and apigenin 5-O-(3′′-O-acetyl)-β-D-glucuronide 3, together with known compounds caffeic acid 4, luteolin 5-O-β-D-glucoside 5 and rosmarinic acid 6 were isolated from the aerial parts of Plectranthus scutellarioides (L.) R. Br. The structures of the new compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1D- and 2D-NMR. Compound 1 inhibited hyaluronidase by 25% at the concentration of 200 μM, compounds 2 and 3 showed inhibitory activity on butyrylcholinesterase better than standard galanthamine at the concentration of 100 μM, and compound 6 is a potent antioxidant with an ORAC value of 2.15 ± 0.12.

Does the presence of leaves on coleus stem cuttings influence their rooting?

Plectranthus scutellarioides (L.) R. Br. is part of the Lamiaceae family and is commonly known as coleus, due to the genus denomination in the past. This ornamental plant species has been gaining importance in the floristic industry due to the great number of exotic cultivars available in the market. Coleus commercial exploitation by seeds is held up by genetic instability, which makes it usually being propagated through semi-hardwood stem cuttings and leaves have great importance in stem cuttings adventitious rooting. The objective of this study was to evaluate the presence or absence of apical leaves and the use of indolebutyric acid (IBA) in coleus stem cuttings rooting. Semi-hardwood coleus stem cuttings with 6 cm long were prepared with or without apical leaves, and then treated with hydroalcoholic solutions (50% v v-1) of 0, 1000 and 2,000 mg L-1 IBA. The propagules were planted in tubes containing vermiculite and kept for 30 days in a greenhouse under intermittent misting (24 ± 2 °C, Relative Humidity 90%) until final evaluation. The experiment was conducted in a completely randomized design, in a 3x2 factorial scheme (3 IBA concentrations x presence and absence of apical leaves). The rooting was evaluated after 30 days. Stem cuttings with and without apical leaves has 100 and 57% rooting, respectively. Stem cuttings rooting did not vary according to IBA concentrations. Roots length and number were higher in stem cuttings with apical leaves in comparison to leafless ones, regardless IBA concentrations. On average, 97.7% sprouting was observed in cuttings with apical leaves, significantly higher rates when compared to the average of 2.2% on leafless cuttings. The presence of apical leaves is fundamental for coleus stem cuttings rooting and the use of IBA is not required for this species propagation.

Effectivity of ethanol extract of miana leaves (coleus scutellarioides [L] Benth.) toward the incision wound healing process on white rat (rattus norvegicus)

Effectivity of ethanol extract of miana leaves (coleus scutellarioides [L] Benth.) toward the incision wound healing process on white rat (rattus norvegicus)

Anticancer potential of rosmarinic acid and its improved production through biotechnological interventions and functional genomics.

Rosmarinic acid (RA) is a highly valued natural phenolic compound that is very commonly found in plants of the families Lamiaceae and Boraginaceae, including Coleus blumei, Heliotropium foertherianum, Rosmarinus officinalis, Perilla frutescens, and Salvia officinalis. RA is also found in other members of higher plant families and in some fern and horned liverwort species. The biosynthesis of RA is catalyzed by the enzymes phenylalanine ammonia lyase and cytochrome P450-dependent hydroxylase using the amino acids tyrosine and phenylalanine. Chemically, RA can be produced via methods involving the esterification of 3,4-dihydroxyphenyllactic acid and caffeic acid. Some of the derivatives of RA include melitric acid, salvianolic acid, lithospermic acid, and yunnaneic acid. In plants, RA is known to have growth-promoting and defensive roles. Studies have elucidated the varied pharmacological potential of RA and its derived molecules, including anticancer, antiangiogenic, anti-inflammatory, antioxidant, and antimicrobial activities. The demand for RA is therefore, very high in the pharmaceutical industry, but this demand cannot be met by plants alone because RA content in plant organs is very low. Further, many plants that synthesize RA are under threat and near extinction owing to biodiversity loss caused by unscientific harvesting, over-collection, environmental changes, and other inherent features. Moreover, the chemical synthesis of RA is complicated and expensive. Alternative approaches using biotechnological methodologies could overcome these problems. This review provides the state of the art information on the chemistry, sources, and biosynthetic pathways of RA, as well as its anticancer properties against different cancer types. Biotechnological methods are also discussed for producing RA using plant cell, tissue, and organ cultures and hairy-root cultures using flasks and bioreactors. The recent developments and applications of the functional genomics approach and heterologous production of RA in microbes are also highlighted. This chapter will be of benefit to readers aiming to design studies on RA and its applicability as an anticancer agent.

Responses of Chemical Functional Groups in Coleus Blumei Roots under Lead Stress with Exogenous Selenium Treatment Using 2-dimensional Correlation FTIR Spectra

Responses of Chemical Functional Groups in Coleus Blumei Roots under Lead Stress with Exogenous Selenium Treatment Using 2-dimensional Correlation FTIR Spectra