Cold Room Temperature Research Articles

Early Cold Stored Platelet Transfusion Following Traumatic Brain Injury: A Randomized Clinical Trial.

To determine the feasibility, efficacy, and safety of cold stored compared to room temperature platelet transfusion in patients with traumatic brain injury. Data demonstrating the safety and efficacy of cold stored platelet transfusion are lacking following traumatic brain injury. A phase 2, randomized, open label, clinical trial was performed at a single U.S. trauma center. Traumatic brain injured patients with positive brain imaging and a need for platelet transfusion received up to two apheresis units of cold stored platelets stored out to 14 days versus standard care room temperature platelet transfusion. The primary outcome was feasibility and the principal clinical outcome for efficacy and safety was the 6-month Glasgow Coma Scale-Extended score. The 6-month Glasgow Outcome Scale-Extended score distributions were not different across cold stored and room temperature platelet arms (OR-1.58, 95%CI 0.71 to 3.54, P=0.27). A lower rate of neurosurgical craniotomy/craniectomy was found for those receiving cold stored platelets (difference -14.4%, 95%CI -26.5% to -2.3%, P=0.03). Adverse event rates did not differ across groups. The storage age of the cold stored product was not associated with outcome differences. In brain injured patients requiring platelet transfusion, early cold stored platelet transfusion is feasible, and did not result in improved 6-month Glasgow Coma Scale-Extended scores. Early cold stored platelet transfusion was associated with a lower rate of neurosurgical operative intervention without an increase in adverse events. The storage age of the cold stored platelet product was not associated with outcome differences. Future phase 3 clinical trials are required to determine clinical outcome differences and safety attributable to cold stored platelet transfusion following traumatic brain injury.

Determination of red colour and lycopene development of <em>Platinum, Padma</em> and <em>Abhiman</em> varieties of tomatoes stored at different temperatures and preparation of tomato puree with watermelon pulp

Lycopene is responsible for red colour development in tomatoes. Since locally grown tomatoes have less lycopene content, it negatively affects the production of tomato sauce at required quality. The aim of this study was to determine the best post-harvest practices to improve the lycopene content in locally grown tomato (Solanum lycopersicon) varieties namely, Platinum, Abhiman, and Padma. They were harvested at the colour break stage and stored at room temperature (32 °C – 34 °C) and cold room temperature (22 °C) for 14 days. Thereafter, they were subjected to analysis, content and colour development (a*) and the obtained values were compared. Lycopene was extracted using a mixture of hexane, ethanol and acetone (2:1:1 ratio) and lycopene analyzed spectrophotometrically. Watermelon is rich in lycopene and pulp extracted from it was incorporated with tomato purees at different ratios to improve the red colour of tomato purees. Results revealed that the average lycopene content of ripe tomatoes at the harvesting stage ranged from 8.34 ± 1.91 to 9.32 ± 2.21 mg/100 g fresh weights. Tomatoes stored at cold room temperatures contained significantly higher amounts of lycopene (37.05 ± 2.50 to 43.03 ± 1.52 mg/ 100 g fresh weight) compared to that of tomatoes stored at room temperatures (16.50 ± 3.10 to 19.04 ± 2.54 mg/100 g fresh weight). Results revealed that the incorporation of watermelon pulp did not contribute to elevating the colour of tomato puree, even at different ratios.

Growth chamber gas dynamics in Cz silicon single crystal growth process

Mathematical simulation results for inert gas (argon) dynamics in the rarefied atmosphere of Redmet-10 Cz single crystal silicon growth furnace chamber have been reported. Gas flows in cold (room temperature) and hot (growth process) chambers have been analyzed. Convective gas flows have been considered for two growth chamber gas supply options: basic supply, i.e., through the top central inlet hole only, and combined, i.e., with additional gas supply through the lateral inlet hole. Gas outlet is through the growth chamber bottom outlet hole for both options. For the cold chamber option, forced convection has been studied using the incompressible ideal gas model. For hot chamber, both the nonisothermal compressible ideal gas model and the weakly compressible gas model in the Boussinesq approximation have been used for studying the vortex structure produced by a joint action of forced and thermal-gravitational convection. The effect of different gas inlet methods on the convective transport of silicon monoxide evaporating from the free melt surface has been discussed.

Changes in the Objective Indices Related to Meat Quality of Porcine Longissimus Dorsi Induced by Different Thawing Methods.

The quality of frozen pork is adversely affected upon thawing. In this study, the influence of various thawing methods for frozen pork, including cold water (18 °C), room temperature (18 °C), and refrigeration (4 °C) thawing, on physicochemical and nutrient substances were examined. The pork samples (a Chinese local breed: Anqing six-end-white pigs), which were thawed through the above conditions, were compared with controls (fresh porcine longissimus dorsi). Analyses were carried out to determine porcine longissimus dorsi shear force, pH value, crude protein content, antioxidant capacity, amino acid content, and fatty acid content. The results indicated that the shear force, pH value, crude protein content, total antioxidant capacity (T-AOC), and total superoxide dismutase (T-SOD) content of the porcine longissimus dorsi muscle significantly decreased via the three thawing methods compared with the control group (p < 0.05). However, malondialdehyde (MDA) content, intramuscular fat content, inosinate and cholesterol content, essential amino acid content, and umami amino acid proportion in the cold thawing group were not significantly different from the control group (p > 0.05), but there were significant differences between the other two groups. The MDA content of the air thawing and hydrostatic thawing groups significantly increased compared with the control group (p < 0.05), with it being 42.6% and 50.8% higher than the control group, respectively. In addition, the monounsaturated fatty acid content in the pork subjected to the three thawing methods significantly increased compared with the control group (p < 0.05), and the monounsaturated fatty acid content after cold thawing and hydrostatic thawing increased by 18.2% and 21.6%, respectively. In conclusion, refrigeration had less influence on the quality of the Anqing six-end-white pork and was the most suitable thawing method. This study provides a theoretical reference for frozen pork preservation for improving food quality and availing its economic benefits.

Innovation in Making Pia Cake Skin with the Addition of Purple Sweet Potato (Ipomea Batatas L) in the Village of Kimi, Nabire District

Purple sweet potato is one type of tuber. Organoleptic test is a test of a food ingredient based on the level of preference. Shelf life is a time span on a product. Objective: To determine the level of consumer preference and shelf life of pia cake skin with the addition of purple sweet potato (Ipomea Batatas L). Methods: Quantitative research with a completely randomized experimental design (CRD). Panelists in this study were 30 untrained panelists, 15 moderately trained panelists. Results: It is known that the results of research conducted on pia cake skin added purple sweet potato (Ipomea Batatas L) have an effect on the taste p-value 0.01 (&lt;0.05), there is an effect on the aroma p-value 0.02 (&lt;0.05), there is an effect on the color p-value 0.00 (&lt;0.05), there is an effect on the texture p-value 0.00 (&lt;0.05), for the shelf life of pia cake skin in treatments X, Y, V and Z at cold temperature and room temperature there is a relationship p-value &lt;0.05. Conclusion: Based on the results of the study, it can be concluded that there are differences in the variables of taste, aroma, color, texture and shelf life of pia cake skin with the addition of purple sweet potato (Ipomea Batatas L) in Kimi Village, which obtained p-value &lt;0.05. Suggestion: There is a need for skills, knowledge related to purple sweet potatoes to increase creativity and the economy of the community.

Extraction of Sulfated Polysaccharides from Ulva sp. Using Acid and Toxicity Testing with the Brine Shrimp Lethality Test (BSLT)

This study investigates the extraction of sulfated polysaccharides from Ulva sp. at pH 3 and evaluates their toxicity using the Brine Shrimp Lethality Test (BSLT) with Artemia salina. Artemia salina is utilized for its rapid life cycle and sensitivity to various chemical compounds, making it an effective bioassay organism for assessing the toxicity of natural extracts. The extraction process was conducted at both hot (80°C) and cold (room temperature) conditions, with the hot extraction yielding higher amounts of polysaccharides. The results indicated a wet extraction yield of 890 mL at 80°C and 800 mL at room temperature, while the highest dry weight yield was achieved at 80°C (0.57 g). The toxicity assessment revealed an LC50 value of 15,815.85 ppm, classifying the sulfated polysaccharides as non-toxic to Artemia salina. Maintaining optimal water quality parameters, including temperature, salinity, pH, and dissolved oxygen levels, is essential for the successful cultivation of Artemia salina. Furthermore, the acidic extraction pH significantly influences the structure and chemical properties of the resulting polysaccharides, emphasizing the importance of this parameter in future applications. These findings support the potential use of sulfated polysaccharides from Ulva sp. as a safe and effective feed ingredient in aquaculture.

The seed endophytic microbe Microbacterium testaceum M15 enhances the cold tolerance and growth of rice (Oryza sativa L.)

The potential of seed endophytic microbes to enhance plant growth and resilience is well recognized, yet their role in alleviating cold stress in rice remains underexplored due to the complexity of these microbial communities. In this study, we investigated the diversity of seed endophytic microbes in two rice varieties, the cold-sensitive CB9 and the cold-tolerant JG117. Our results revealed significant differences in the abundance of Microbacteriaceae, with JG117 exhibiting a higher abundance under both cold stress and room temperature conditions compared to CB9. Further analysis led to the identification of a specific cold-tolerant microbe, Microbacterium testaceum M15, in JG117 seeds. M15-inoculated CB9 plants showed enhanced growth and cold tolerance, with a germination rate increase from 40 % to 56.67 % at 14℃ and a survival rate under cold stress (4℃) doubling from 22.67 % to 66.67 %. Additionally, M15 significantly boosted chlorophyll content by over 30 %, increased total protein by 16.31 %, reduced malondialdehyde (MDA) levels by 37.76 %, and increased catalase activity by 26.15 %. Overall, our study highlights the potential of beneficial endophytic microbes like M. testaceum M15 in improving cold tolerance in rice, which could have implications for sustainable agricultural practices and increased crop productivity in cold-prone regions.

Performance profile of Cold Storage Using R32 as Refrigerant for Traditional Fishing Boat with Photovoltaic as Energi Source

This paper discusses the performance of cold storage using R32 refrigerant. R32 is one of the recommended refrigerant with the main advantage low ODP and GWP (Global Warming Potential) value of around 0 and 675. However, because of this refrigerant classified as a new refrigerant, the implementation is limited to air conditioning and heat pump. In this paper, R32 will be tested for cold storage applications. The cold storage performance will be studied about the achieved temperature, power consumption, cooling capacity and Coefficient of Performance without load. The testing was carried out in 2 ways, cold storage testing on a lab scale and direct testing on a 5 GT fishing boat. The performance results show that both tests on a lab scale and tests directly on a fishing boat without a load can reach a cold storage room temperature of -18oC. Meanwhile, the compressor power consumption supplied by photovoltaic is 0.653-0.776 kW. Based on the test results, shows that R32 has a positive possibility of being applied to cold storage.

Quality Control of Kinasih Bread Products Using Statistical Quality Control and Failure Mode and Effect Analysis Methods

Roti Kinasih UMKM is a business that focuses on the culinary sector by producing various types of bread. The business process carried out at this UMKM is a Make to Order system where products are made after receiving orders from customers. One problem that often arises is the inability to control product quality. Having defective products has a big impact on MSMEs because the costs incurred increase. Based on the existing problems, the Statistical Quality Control and Failure Mode And Effect Analysis methods were chosen to determine the factors causing product defects in UMKM Roti Kinasih. The aim of this research is to determine the level of defects in Kinasih bread products and carry out quality control measures using statistical methods. After processing and analyzing the data, it was found that the burnt bread defect and the sinking bread defect were said to be in an uncontrolled condition and needed recommendations for improvement. The highest RPN value for the burnt bread defect was 245 with the overtime baking failure mode, while the RPN value for the sinking bread defect was 175 with the too cold room temperature failure mode.

Peroperative Cooling in Rhinoplasty: Does it Differ?

The main causes of ecchymosis and edema are osteotomy (bone manipulation), dissection of subcutaneous tissue, and skin manipulation in the rhinoplasty procedure. Eyelid edema following surgery can potentially affect visual acuity, particularly during the initial twenty-four hours after the procedure. These may also delay the patient’s return to their normal social life therefore hampering their quality of life. Various surgical and medical methods have been reported to address these issues. This study aimed to compare the effects of using cold saline (0–4 °C) versus room temperature saline (20–25 °C) irrigation throughout the surgery on postoperative edema, ecchymosis, and pain. Fifty patients who underwent open-approach primary rhinoplasty between August 2022 and August 2023 at a tertiary academic center were included. Fifty patients were randomly divided into two groups depending on using cold saline (0–4 °C) (group 1) or room temperature saline (20–25 °C) (group 2) during surgical site irrigation. Patients were assessed for pain, edema, and bruising using a VAS (Visual Analog Scale) on the second and seventh postoperative days. Visual analog score (VAS) was used for subjective outcome analyses. Each patient scored the severity of their periorbital ecchymosis on day two and seven. Periorbital ecchymosis was also evaluated on the second and seventh postoperative days using the SPREE (Surgeon Periorbital Rating of Edema and Ecchymosis) scale. On the second postoperative day, the VAS pain score in group 1, where cold water was used, was found to be statistically and significantly different from the control group (group 2) (p < 0.05). However, there was no statistically significant difference between both groups when comparing the VAS pain scores on the seventh postoperative day. Regarding the VAS ecchymosis score on the seventh postoperative day, there was a statistically significant difference favoring group 1 (p < 0.05). The SPREE scale data also indicated that group 1 had significantly lower scores on the seventh day (p < 0.05). While the SPREE scores on the second day were lower in group 1 than in group 2, this difference did not reach statistical significance (p = 0.061). The findings from our study show that cold saline irrigation may contribute to intraoperative hemostasis by inducing local vasoconstriction. We observed that intraoperative bleeding decreased with the use of cold saline. This approach has the potential to improve patient satisfaction and overall quality of life by reducing postoperative ecchymosis without significantly increasing the cost of the surgical procedure.Level of Evidence III This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

The Optimal Storage Condition and Storage Time of Umbilical Cord Blood from Collection to Preparation

To explore the optimal storage condition and time of umbilical cord blood from collection to preparation. Collect cord blood samples from 30 healthy newborns, with each new born's umbilical cord blood was divided into two parts on average. One part was stored in cold storage (4 ℃) and the other was stored at room temperature (20-24 ℃). Samples were taken at 24, 36, 48, 60 and 72 h, respectively, total nucleated cells (TNC) count and TNC viability was analyzed. Flow cytometry was used to detect the ratio of viable CD34+ cells to viable CD45+ cells and viability of CD34+ cells, and colony-forming unit-granulocyte-macrophage (CFU-GM) count was performed by hematopoietic progenitor cell colony culture. The change trend of each index over time was observed, and the differences in each index was compared between cold storage and room temperature storage under the same storage time. The TNC count (r 4 ℃=-0.9588， r 20-24 ℃=-0.9790), TNC viability (r 4 ℃=-0.9941， r 20-24 ℃=-0.9970), CD34+ cells viability (r 4 ℃=-0.9932， r 20-24 ℃=-0.9828) of cord blood stored in cold storage (4 ℃) and room temperature storage (20-24 ℃) showed a consistent downward trend with the prolongation of storage time. The percentage of viable CD34+ cells (r 4 ℃=0.9169， r 20-24 ℃=0.7470) and CFU-GM count (r 4 ℃=-0.2537， r 20-24 ℃=-0.8098) did not show consistent trends. When the storage time was the same, the TNC count, TNC viability, CD34+ cells viability and CFU-GM count of cord blood stored in cold storage were higher than those stored at room temperature. Under the same storage time (24, 36, 48, 60 or 72 h), TNC viability in room temperature storage was significantly lower than that in cold storage (P <0.001), but TNC count, percentage of viable CD34+ cells and CFU-GM count were not significantly different between room temperature storage and cold storage. When stored at room temperature for 24 h and 36 h, the viability of CD34+ cells was significantly lower than that in cold storage (P <0.001, P <0.01), when the storage time for 48, 60 and 72 h, there was no significant difference in the CD34+ cells viability between room temperature storage and cold storage. It is recommended that cord blood be stored in cold storage (4 ℃) from collection to preparation, and processed as soon as possible.

Intestinal dual-specificity phosphatase 6 regulates the cold-induced gut microbiota remodeling to promote white adipose browning

Gut microbiota rearrangement induced by cold temperature is crucial for browning in murine white adipose tissue. This study provides evidence that DUSP6, a host factor, plays a critical role in regulating cold-induced gut microbiota rearrangement. When exposed to cold, the downregulation of intestinal DUSP6 increased the capacity of gut microbiota to produce ursodeoxycholic acid (UDCA). The DUSP6-UDCA axis is essential for driving Lachnospiraceae expansion in the cold microbiota. In mice experiencing cold-room temperature (CR) transitions, prolonged DUSP6 inhibition via the DUSP6 inhibitor (E/Z)-BCI maintained increased cecal UDCA levels and cold-like microbiota networks. By analyzing DUSP6-regulated microbiota dynamics in cold-exposed mice, we identified Marvinbryantia as a genus whose abundance increased in response to cold exposure. When inoculated with human-origin Marvinbryantia formatexigens, germ-free recipient mice exhibited significantly enhanced browning phenotypes in white adipose tissue. Moreover, M. formatexigens secreted the methylated amino acid Nε-methyl-L-lysine, an enriched cecal metabolite in Dusp6 knockout mice that reduces adiposity and ameliorates nonalcoholic steatohepatitis in mice. Our work revealed that host-microbiota coadaptation to cold environments is essential for regulating the browning-promoting gut microbiome.

Hemoperitoneum Related with Peritoneal Dialysis in A Female Adolescent: it is Not as Frightening as it Seems

Although hemoperitoneum is a benign and common complication of chronic peritoneal dialysis the apperance of the effluent will be devastating for the patients. In women of reproductive age group gynecological causes are the common etiological factors. A 17 years old girl who had been on automated peritoneal dialysis for six months was admitted because of bloody effluent lasting for two days. At admission she described mild abdominal pain; physical examination was unremarkable and bleeding time and coagulation profile was normal. Abdominal ultrasound revealed hemorrhagic cysts on left ovary. Peritoneal effluent was cleared by using rapid exchanges with room temperature dialysate. Hemoperitoneum is a well recognized complication of peritoneal dialysis in women of reproductive age.Abdominal ultrasound is the first, easily applicable and reliable diagnostic modality in order to detect underlying causes of hemoperitoneum. Performing rapid exchanges with cold-room temperature dialysate is the mainstay of treatment.

Color Stability and Antioxidant Activity of Red Roselle (Hibiscus Sabdariffa L.) Calyx Infuse

The roselle flower (Hibiscus sabdariffa L.), is known as a potential functional food source because of its antioxidant and antibacterial properties. Apart from that, it is also used as a natural dye because of its red color. Antioxidant compounds in roselle flowers include neo-chlorogenic acid, chlorogenic acid, cryptochlorogenic acid, routine, and isoquercitrin. The high content of these antioxidant compounds often causes color instability. For this reason, this research will analyze the effect of temperature and long storage on color changes and the antioxidant activity of a red roselle flower infusion. Storage conditions are carried out with three temperature variations, namely cold temperature (2–8 oC), cool temperature (8–15 oC), and room temperature (15–30oC), with a long storage of 8 days. The stability parameters used are color changes and activity as an antioxidant. Color changes were measured using a chromameter, while the antioxidant activity was measured by the IC50 value using the DPPH method. The principle of this method is that a purple color change reaction based, which fades, and then the inhibitory intensity against DPPH is measured using UV-Vis spectrophotometer. It was found that the color values have significant differences visible from the first day storage at the room temperature (30oC), cool temperature (11oC), and cold temperature (6 oC). So, there was an influence of temperature and long storage on the color change of boiled red roselle flower calyx. A significant decrease in activity was seen on the first day storage at room temperature (30 oC) and third day storage at cool temperature (11 oC). Meanwhile, at cold temperatures (6 oC), there was no significant effect on the reduction in activity from boiling red roselle flower calyx for up to 8 days of storage

Efficiency Improvement of Double Pipe Heat Exchanger by using TiO2/water Nanofluid

Heat exchangers are commonly utilized to transfer heat between two fluids in a number of industries. However, parameters such as fluid flow velocity, temperature difference, and thermal conductivity limit their efficiency. Researchers have investigated the use of nanofluids - fluids containing nanoparticles that boost thermal characteristics - to improve the performance of heat exchangers. The use of nanofluids can improve the efficiency of double-pipe heat exchangers. However, research on the influence of TiO2/water nanofluid on the performance of double-pipe heat exchangers is insufficient. The purpose of this research is to investigate the impact of TiO2/water nanofluid on the efficiency of a double-pipe copper counter-flow heat exchanger. A double-pipe copper counter-flow heat exchanger using cold (room temperature) and hot (70°C) water as working fluids was used in an experimental investigation. They created nanofluids by adding varying concentrations (0.1%, 0.3%, and 0.5%) of TiO2 nanoparticles to water and measuring their heat conductivity and viscosity. They then calculated the overall heat transfer coefficient and efficacy by measuring the input and outlet temperatures as well as the flow rates of both fluids. It was discovered that adding TiO2 nanoparticles to water enhanced its heat conductivity and viscosity substantially. The overall heat transfer coefficient increased up to 0.3% but declined at 0.5% nanoparticle concentration. At a nanoparticle concentration of 0.3%, the maximum effectiveness was attained, with a corresponding increase in efficiency of up to 23%. The scientists found that using TiO2/water nanofluid to improve the efficiency of double-pipe heat exchangers is a viable option.

Evaluation of High-Alert Drug Storage in The Central Pharmacy of RSUD Ratu Zalecha Martapura

Background: High-alert drugs require special attention when used because they can cause adverse reactions. High-alert drugs are classified into 3, namely, concentrated electrolytes, LASA, and cytostatics. Objectives: This study aimed to determine the evaluation of High-alert drug storage at the central pharmacy of Ratu Zalecha Martapura Hospital. Material and Methods: The research was conducted using a descriptive method, with observational data collection using an observation checklist sheet. The population and study sample took data on all High-alert medicines at the central pharmacy of Ratu Zalecha Martapura Hospital. Results: High alert drug storage was based on SOP, placed in a central pharmacy using the FIFO and FEFO methods, was placed in a separate place based on cytotoxic drug groups, electrolyte concentrates, and others. High alert drug storage according to storage temperature requirements for each drug (cold temperature and room temperature), was labeled "high alert" and electrolyte concentrates were only available in pharmaceutical installations. Conclusions: The conclusion of this research was High-alert drug storage in the Pharmaceutical installation of Ratu Zalecha Martapura Hospital have been qualified seven (7) criteria based on Minister of Health Decision Nu. 1128 of 2022 and Minister of Health Regulation Nu. 72 of 2016. Good and correct drug storage is very important in order to maintain the quality and quality of drugs.

Can Chitosan Applications in Pre- and Post-Harvest Affect the Quality and Antioxidant Contents of Red Raspberries?

Red raspberry fruit production is increasing worldwide due to the growing consumer interest in foods with high antioxidant properties. However, raspberries are very perishable fruit with a short shelf life. Chitosan applications have shown promising results in promoting the storage of different berry fruit. This work aimed at analysing the effects of chitosan applied in pre- and/or post-harvest on the quality and antioxidant properties of raspberry fruit during cold storage and room temperature conditions (i.e., 6 d at 4 °C and 3 d at 20 °C, respectively). Pre-harvest chitosan applications reduced fruit weight loss during cold storage and room temperature conditions but also reduced fruit decay at room temperature conditions. At the end of the whole storage conditions, chitosan-coated raspberries at pre-harvest were brighter than those of the uncoated control fruit. Furthermore, pre-harvest treatments with chitosan increased the total phenol and anthocyanin contents and promoted the highest total antioxidant activity compared with other treatments. After cold storage, post-harvest chitosan application drastically reduced the development of fungi that cause fruit decay, thus minimizing the potential risk of mycotoxin production. Overall, this study demonstrates that applications of chitosan in pre-harvest are sufficient to ensure the goal of maintaining and/or increasing fruit quality and antioxidant properties during cold storage and room temperature conditions.

Effects of Commercial Natural Compounds on Postharvest Decay of Strawberry Fruit

Gray mold and Rhizopus rot, which is caused by Botrytis cinerea and Rhizopus stolonifer, respectively, are the most destructive forms of postharvest decay of the strawberry fruit. In this work, we tested the effectiveness of the control on the postharvest decay of the strawberry fruit (Fragaria × ananassa Duch cv. ‘Monterey’) following postharvest applications of six commercial natural compounds: chitosan-based coating compound (1% of ‘ChitP’, ‘ChitS’, ‘ChitK’, ‘ChitO’), commercial essential oil (EOs) products based on grapefruit seed extract (0.5% of ‘GraFr’), sweet orange (0.5% of ‘SwOr’), a product that included eugenol, geraniol, and thymol EO, (0.4% of ‘EuGeTh’), an organic compound as humic acid (0.5% w/v of ‘HuAc’), and, lastly, methyl jasmonate plant growth regulator (1% v/v ‘MeJA’). Strawberries were dipped in solution for 30 s and incubated at room temperature (20 ± 0.5 °C) or at cold storage conditions (4 ± 0.5 °C) following 4 days of shelf life at 20 °C. The treatments with ‘ChitP’, ‘ChitS’, and ‘ChitO’ provided ~30%–40% reduction of gray mold in cold storage conditions, while the ‘MeJA’, ‘SwOr’, and ‘GraFr’ with high activities of volatile substances were more effective at controlling gray mold at room temperature. ‘HuAc’, ‘ChitK’, and ‘ChitO’ were more effective at controlling Rhizopus rot in both cold storage (~50%) and room temperature conditions.

Energetic macroscopic representation of a mechanical compression-assisted hybrid thermochemical cycle exploiting low grade heat for cold applications

This paper describes an original model based on The Energetic Macroscopic Representation (EMR) to develop a control structure of an innovative compression-assisted hybrid thermochemical cooler driven by low-grade thermal energy. Hybrid thermochemical cooler with mechanical compression have a wider operating temperature range than conventional thermochemical processes. This makes it possible to exploit lower grade heat sources and/or produce cold at lower temperatures. In addition, the ability to control compressor speed increases thermochemical process controllability to respond to load or source variations. Nodal modeling of each process component is developed according to the EMR formalism. A parametric identification and validation of this process model is then carried out using experimental data. The deviation from the experimental data is lower than 1bar for the reactor, condenser and evaporator pressure, lower than 0.1 for the reaction advancement and lower than 2°C for the reactor wall temperature. The EMR model is then inverted to obtain a process control law for maintaining a cold room at -18°C. Despite the variation in cooling demand from a minimum of 100W to a maximum of 700W, the compressor's control structure was able to regulate its speed of rotation to maintain the cold room temperature at -18±0.5°C for 24h.

Formulasi Obat Kumur Minyak Atsiri Sereh Wangi (Cymbopogon nardus (L.) Rendle) dan Uji Aktivitas Antibakteri terhadap Streptococcus mutans

Mouthwash is a liquid, aqueous composition that is primarily used to treat oral conditions, as well as maintain oral health. The purpose of this study was to prove the antibacterial efficacy of citronella essential oil against Streptococcus mutans bacteria made in mouthwash formulations. Citronella essential oil was analyzed using GC-MS and then formulated into a mouthwash with concentrations of 1.5%, 3% and 4.5% at cold temperatures (2-8°C) and room temperature (20-25°C).The test results were evaluated, and analyzed using One Way ANOVA and Post Hoc Tukey. The GC-MS results showed that 3 main compounds were detected, namely Citronellol (11.69%), Geraniol (15.59%), and Citronellal (24.57%). The mouthwash formulation did not show any organoleptic change in physical preparation, was homogeneous, had a pH range of 4.91-5.18 at cold temperature and pH 5.02-5.23 at room temperature, had a viscosity consistency that met the standard of 0.8 -1 at cold temperature and room temperature, and no separation was found, such as the presence of precipitate in each treatment. Formula 3 is considered to have medium inhibition compared to formulas 1 and 2 with an average inhibition power at cold temperature formula 6.73 while at room temperature formula 7.35. Briefly, the formulation is stable both at cold temperature (2-8°C) and room temperature (20-25°C) with moderate inhibition at a concentration of 4.5%.