In the process of eddy current testing (ECT) of surface and subsurface defects of aviation aluminum alloy plates, the setting of parameters is important to the test results. Inappropriate test parameters can cause false detection or even missed detection of defects. To address this problem, the effects of probe type, coil size, and excitation frequency on the accurate identification and quantitative evaluation of surface and subsurface defect detection were studied and analyzed in this study to determine the best testing parameters. The experimental results show that the absolute probe with an outer radius of 3.3 mm has better detection performance for aviation aluminum alloy plate defects. There are different optimal excitation frequency ranges for the surface and subsurface defects. An excitation frequency of 80 kHz to 90 kHz can be used for the detection of unknown defects.