Coherence Tomography System Research Articles

In Vivo Identification of Skin Photodamage Induced by Fractional CO2 and Picosecond Nd:YAG Lasers with Optical Coherence Tomography

Fractional laser treatment is commonly used for dermatological applications, enabling effective induction of collagen regeneration and significantly reducing recovery time. However, it is challenging to observe laser-induced photodamage beneath the tissue surface in vivo, making the non-invasive evaluation of treatment outcomes difficult. For in vivo real-time study of the photodamage induced by fractional pulsed CO2 and Nd:YAG lasers commonly utilized for clinical therapy, a portable spectral-domain optical coherence tomography (SD-OCT) system was implemented for clinical studies. The photodamage caused by two lasers, including photothermal and photoacoustic effects, was investigated using OCT, together with the correlation between photodamage and exposure energy. Additionally, to investigate the change in the optical properties of tissue due to photodamage, the attenuation coefficients and damaged areas of normal skin and laser-treated skin were estimated for comparison. Finally, the recovery of the exposed skin with both lasers was also compared using OCT. The results show that OCT can be a potential solution for in vivo investigation of laser-induced tissue damage and quantitative evaluation.

Image Reconstruction Using Autofocus in Single-Lens System

To reconstruct the wavefront in a single-lens coherent diffraction imaging (CDI) system, we propose a closed-loop cascaded iterative engine (CIE) algorithm based on the known information of the imaging planes. The precision of diffraction distance is an important prerequisite for a perfect reconstruction of samples. For coherent diffraction imaging with a lens, autofocus is investigated to accurately determine the object distance and image distance. For the case of only the object distance being unknown, a diffuser is used to scatter the coherent beam for speckle illumination to improve the performance of autofocus. The optimal object distance is obtained stably and robustly by combing speckle imaging with clarity evaluation functions. SSIM and MSE, using the average pixel value of the reconstructed data set as a reference, are applied on two-unknown-distance autofocus. Simulation and experiment results are presented to prove the feasibility of the CIE and proposed auto-focusing method.

Self-witnessing coherent imaging for artifact removal and noise filtering

In situ coherent imaging provides on-the-fly measurement of morphology changes during high-power laser processing (such as welding and additive manufacturing) at high speeds (exceeding 300 kHz line rates) but suffers from speckle and image artifacts particular to interferometric imaging. We present a self-witnessing inline coherent imaging system that incorporates a second imaging channel to allow complete removal of autocorrelation artifacts, and an increase in signal to noise by a factor of two, with no loss of imaging rate or spatial resolution and no need for balanced detection or specialized light sources. When applied to laser welding of 316L stainless steel, the keyhole tracking rate is increased by as much as 67%.

Polarimetric SAR Despeckling With Convolutional Neural Networks

Coherent imaging systems such as synthetic aperture radar (SAR) are subject to speckle, the reduction of which is an active area of study. Methods based on deep convolutional neural networks (CNNs) have recently demonstrated state-of-the-art performance in the removal of additive noise from natural images and speckle from single-channel SAR images. The application of deep learning to multichannel SAR modalities such as polarimetric SAR (PolSAR) is complicated in part by the nature of the data as images of complex-valued covariance matrices. In this article, we propose a CNN-based PolSAR despeckling approach that uses an invertible transformation involving a matrix logarithm to facilitate CNN processing of the PolSAR data. A residual learning strategy is adopted, in which the CNN is trained to identify the speckle component which is then removed from the corrupted image. The experimental results on simulated and measured PolSAR data show the proposed approach to markedly reduce speckle and preserve scene features.

Statistical Analysis of CyGNSS Speckle and Its Applications to Surface Water Mapping

The Global Navigation Satellite System reflectometry (GNSS-R) technique has demonstrated its potential for terrestrial applications. Over inland water bodies, the dominance of coherent components in GNSS-R has been widely recognized. Nevertheless, little attention is given to GNSS-R speckle, which is inherent to coherent imaging systems. In this study, taking the multiplicative speckle into account, we regard GNSS-R coherent scattering as a statistical distribution. First, the expression of the statistical distribution is identified and parameterized using observations from the Cyclone Global Navigation Satellite System (CyGNSS). The results suggest that the power tends to obey a three-degrees-of-freedom distribution model. Second, the multilook statistics of CyGNSS, such as the mean value and the coefficient of variation (CV), are analyzed on different spatial–temporal scales. Finally, we realize surface water mapping using multilook statistics. Comparison with the state-of-the-art algorithms shows that the proposed method can effectively improve the goodness of water mapping, with higher overall accuracies (~0.97) and F1 scores (~0.60). This study provides new insights into future GNSS-R land observations.

Speckle noise reduction in coherent imaging systems via hybrid median–mean filter

Images recorded by coherent imaging systems, including laser-based photography, digital holography (DH), and digital holographic microscopy (DHM), are severely distorted by speckle noise. We present a single-shot image processing method to reduce the speckle noise, coined hybrid median–mean filter (HM2F), which is based on the average of conventional median-filtered images with different kernel sizes. The synergic combination of the median filter and mean approach provides a denoised image with reduced speckle contrast while the spatial resolution is kept at up to 97% of the original value. The HM2F method is compared with the conventional median filter approach, the 3D block matching filter, the nonlocal means filter, the 2D windowed Fourier transform filter, and the Wiener filter using different speckle-distorted images to benchmark its performance. Based on the experimental results and the simplicity of the technique, HM2F is proposed as an effective denoising tool for reducing the speckle noise in laser-based photography, DH, and DHM.

In-Vivo Measurement of Ocular Deformation in Response to Ambient Pressure Modulation.

A novel approach is presented for the non-invasive quantification of axial displacement and strain in corneal and anterior crystalline lens tissue in response to a homogenous ambient pressure change. A spectral domain optical coherence tomography (OCT) system was combined with a custom-built set of swimming goggles and a pressure control unit to acquire repetitive cross-sectional scans of the anterior ocular segment before, during and after ambient pressure modulation. The potential of the technique is demonstrated in vivo in a healthy human subject. The quantification of the dynamic deformation response, consisting of axial displacement and strain, demonstrated an initial retraction of the eye globe (−0.43 to −1.22 nm) and a subsequent forward motion (1.99 nm) in response to the pressure change, which went along with a compressive strain induced in the anterior crystalline lens (−0.009) and a tensile strain induced in the cornea (0.014). These mechanical responses appear to be the result of a combination of whole eye motion and eye globe expansion. The latter simulates a close-to-physiologic variation of the intraocular pressure and makes the detected mechanical responses potentially relevant for clinical follow-up and pre-surgical screening. The presented measurements are a proof-of-concept that non-contact low-amplitude ambient pressure modulation induces tissue displacement and strain that is detectable in vivo with OCT. To take full advantage of the high spatial resolution this imaging technique could offer, further software and hardware optimization will be necessary to overcome the current limitation of involuntary eye motions.

Development and assessment of an x-ray tube-based multi-beam x-ray scatter projection imaging system.

Coherent scatter x-ray imaging systems are sensitive to material structure and chemical composition, and generate soft-material images with contrast superior to conventional transmission x-ray imaging. For practicality in medical or security applications, the image data acquisition time should be <10 min. Our approach is a multi-beam projection imaging design. Previously, as a development stage, we implemented a synchrotron-based system with five coplanar pencil beams and continuous motion of the object. In the work reported here, we developed a more practical coherent scatter projection imaging system using a conventional x-ray tube source. The object is irradiated by an array of up to three rows by five columns of pencil beams, and motorized stages translate the object through the beams for step-and-shoot acquisition. For the same tube loading, broad spectrum beams, such as 110 kVp filtered with 2.25 mm Al, were found to provide a higher signal-difference-to-noise ratio between soft materials in scatter images than lower kVp, more heavily filtered beams that have a narrower, lower intensity spectrum. The shortest acquisition time for a 6.0 × 10.0 cm2 object with 6000 pixels was 8.8 min. The width of a sharp edge in the scatter image was consistent with the pencil beam diameter. Contrast-detail performance was similar to our synchrotron-based system. In this first x-ray tube-based system, for simplicity, the transmitted x rays are measured through attenuators using the same flat-panel detector that measures scattered x rays. As a result, the primary image quality was reduced.

Ultrafast third-order nonlinear optical properties of self-assembled PCPDTPhSO3Na/C70-(EDA)8 periodically overlapping film

A set of ultrathin multilayer films with alternated layers containing Poly[4,4-bis(4-sulfobutyl)-4H-cyclopenta[2,1-b:3,4-b′]dithiophene-2,6-diyl]-1,4-phenylene sodium sa (PCPDTPhSO3Na) as donor and amine group modified fullerene derivative C70-(ethylenediamine)8 (C70-(EDA)8) as acceptor were prepared using layer-by-layer assembly technique on a quartz substrate. Driven by the electrostatic force, C70-(EDA)8 and PCPDTPhSO3Na were alternately adsorbed on the substrate to form donor–acceptor periodically overlapping structure. The growth of the film was monitored by UV-Vis absorption spectra, showing regularly consecutive deposition process. The ultrafast third-order nonlinear optical properties of the films were investigated via a 4f coherent imaging system with phase object (NIT-PO) with 190 fs laser pulses at 400 nm. Experimental results demonstrate that the ultrathin films exhibit excellent self-focusing effect (n2∼4.02 × 10−16 m2/W) and reverse saturable absorption properties (β∼7.75 × 10−10 m/W). Our studies indicate that the PCPDTPhSO3Na/C70-(EDA)8 film has significant potential for applications in future nonlinear optical devices.

In vivo human retinal swept source optical coherence tomography and angiography at 830\xa0nm with a CMOS compatible photonic integrated circuit

Photonic integrated circuits (PIC) provide promising functionalities to significantly reduce the size and costs of optical coherence tomography (OCT) systems. This paper presents an imaging platform operating at a center wavelength of 830 nm for ophthalmic application using PIC-based swept source OCT. An on-chip Mach–Zehnder interferometer (MZI) configuration, which comprises an input power splitter, polarization beam splitters in the sample and the reference arm, and a 50/50 coupler for signal interference represents the core element of the system with a footprint of only (12 times 5);{text {mm}}^2. The system achieves 94 dB imaging sensitivity with 750 upmu W on the sample, 50 kHz imaging speed and 5.5 upmu m axial resolution (in soft tissue). With this setup, in vivo human retinal imaging of healthy subjects was performed producing B-scans, three-dimensional renderings as well as OCT angiography. These promising results are significant prerequisites for further integration of optical and electronic building blocks on a single swept source-OCT PIC.

Reconfigurable Metasurface for Image Processing.

Optical Fourier transform-based processing is an attractive technique due to the fast processing times and large-data rates. Furthermore, it has recently been demonstrated that certain Fourier-based processors can be realized in compact form factors using flat optics. The flat optics, however, have been demonstrated as static filters where the operator is fixed, limiting the applicability of the approach. Here, we demonstrate a reconfigurable metasurface that can be dynamically tuned to provide a range of processing modalities including bright-field imaging, low-pass and high-pass filtering, and second-order differentiation. The dynamically tunable metasurface can be directly combined with standard coherent imaging systems and operates with a numerical aperture up to 0.25 and over a 60 nm bandwidth. The ability to dynamically control light in the wave vector domain, while doing so in a compact form factor, may open new doors to applications in microscopy, machine vision, and sensing.

Physics-Based Modelling and Simulation of Multibeam Echosounder Perception for Autonomous Underwater Manipulation

One of the key distinguishing aspects of underwater manipulation tasks is the perception challenges of the ocean environment, including turbidity, backscatter, and lighting effects. Consequently, underwater perception often relies on sonar-based measurements to estimate the vehicle’s state and surroundings, either standalone or in concert with other sensing modalities, to support the perception necessary to plan and control manipulation tasks. Simulation of the multibeam echosounder, while not a substitute for in-water testing, is a critical capability for developing manipulation strategies in the complex and variable ocean environment. Although several approaches exist in the literature to simulate synthetic sonar images, the methods in the robotics community typically use image processing and video rendering software to comply with real-time execution requirements. In addition to a lack of physics-based interaction model between sound and the scene of interest, several basic properties are absent in these rendered sonar images–notably the coherent imaging system and coherent speckle that cause distortion of the object geometry in the sonar image. To address this deficiency, we present a physics-based multibeam echosounder simulation method to capture these fundamental aspects of sonar perception. A point-based scattering model is implemented to calculate the acoustic interaction between the target and the environment. This is a simplified representation of target scattering but can produce realistic coherent image speckle and the correct point spread function. The results demonstrate that this multibeam echosounder simulator generates qualitatively realistic images with high efficiency to provide the sonar image and the physical time series signal data. This synthetic sonar data is a key enabler for developing, testing, and evaluating autonomous underwater manipulation strategies that use sonar as a component of perception.

Resolution Enhancement in Coherent Diffraction Imaging Using High Dynamic Range Image

In a coherent diffraction imaging (CDI) system, the information of the sample is retrieved from the diffraction patterns recorded by the image sensor via multiple iterations. The limited dynamic range of the image sensor restricts the resolution of the reconstructed sample information. To alleviate this problem, the high dynamic range imaging technology is adopted to increase the signal-to-noise ratio of the diffraction patterns. A sequence of raw diffraction images with differently exposure time are recorded by the image sensor. Then, they are fused to generate a high quality diffraction pattern based on the response function of the image sensor. With the fused diffraction patterns, the resolution of the coherent diffraction imaging can be effectively improved. The experiments on USAF resolution card is carried out to verify the effectiveness of our proposed method, in which the spatial resolution is improved by 1.8 times using the high dynamic range imaging technology.

Ultrafast third order nonlinear optical properties of self-assembled indium phthalocyanine multilayer films

An ultrathin composite films with alternated layers containing anionic lithium tetracarboxyl indium phthalocyanine chloride (ClInPc(COOLi)4) and cationic poly(diallydimethyl ammonium chloride) (PDDA) were prepared using layer-by-layer assembly technique. The resulting films were characterized by Ultraviolet-visible and Fourier transform infrared spectroscopy, showing regularly consecutive adsorption. The ultrafast third-order nonlinear optical properties of the films were investigated via a 4f coherent imaging system with phase object with 190 fs laser pulses at 532nm. The results demonstrate that the ultrathin films exhibited excellent self-focusing effect (n2 ∼ 8.86 × 10−17 m2/W) and reverse saturable absorption properties (β ∼ 5.19 × 10−10 m/W), while the nonlinear optical response of the same composite in aqueous solution were found to be negligible. Our results indicate that the ClInPc(COOLi)4/PDDA film is a promising candidate for future nonlinear optical devices.

Development and application of a tender X-ray ptychographic coherent diffraction imaging system on BL27SU at SPring-8.

Ptychographic coherent diffraction imaging (CDI) allows the visualization of both the structure and chemical state of materials on the nanoscale, and has been developed for use in the soft and hard X-ray regions. In this study, a ptychographic CDI system with pinhole or Fresnel zone-plate optics for use in the tender X-ray region (2-5 keV) was developed on beamline BL27SU at SPring-8, in which high-precision pinholes optimized for the tender energy range were used to obtain diffraction intensity patterns with a low background, and a temperature stabilization system was developed to reduce the drift of the sample position. A ptychography measurement of a 200 nm thick tantalum test chart was performed at an incident X-ray energy of 2.500 keV, and the phase image of the test chart was successfully reconstructed with approximately 50 nm resolution. As an application to practical materials, a sulfur polymer material was measured in the range of 2.465 to 2.500 keV including the sulfur K absorption edge, and the phase and absorption images were successfully reconstructed and the nanoscale absorption/phase spectra were derived from images at multiple energies. In 3 GeV synchrotron radiation facilities with a low-emittance storage ring, the use of the present system will allow the visualization on the nanoscale of the chemical states of various light elements that play important roles in materials science, biology and environmental science.

Anterior Chamber Characteristics, Endothelial Parameters, and Corneal Densitometry After Descemet Stripping Automated Endothelial Keratoplasty in Patients With Fuchs Dystrophy.

PurposeTo compare anterior segment parameters in patients with Fuchs endothelial dystrophy (FED) who underwent Descemet stripping automated endothelial keratoplasty (DSAEK) in one eye and no corneal surgery in the fellow eye.MethodsThis prospective study was conducted on 28 eyes of 14 patients with FED who underwent DSAEK in one eye at least one year prior (DSAEK group) and no corneal surgery in the fellow eye (control group). Each eye was analyzed with the anterior segment optical coherence tomography, specular microscopy, and Scheimpflug imaging systems. Data were compared between the two groups.ResultsThe mean age of the patients was 76.9 7.0 years. There were no statistically significant differences in the mean central corneal thickness (CCT), central anterior chamber depth, anterior chamber angle parameters, cylinder and keratometry values between two groups (all P-values 0.05). The paracentral corneal thickness, corneal volume, endothelial cell density, and hexagonal cell ratio measurements were statistically significantly higher in the DSAEK group than the control (all P-values < 0.05), and anterior chamber volume in the DSAEK group was significantly less than the control (P = 0.046). While posterior and total corneal densitometry values in the DSAEK group were statistically significantly lower than the control (P < 0.001 and P = 0.011, respectively), there were no statistically significant differences in the anterior or middle corneal densities (P = 0.108 and P = 0.134, respectively).ConclusionWe found that total corneal densitometry value decreased in DSAEK group. Although DSAEK surgery did not affect the anterior chamber angle parameters, it reduced the anterior chamber volume and increased the corneal volume and paracentral corneal thickness due to the addition of the DSAEK graft.

Speckle Reduction Using Adaptive Receive-Side Compounding.

A typical approach to reduce speckle in coherent imaging systems is to average same-target images with different speckle realizations. We study settings where such realizations come from applying different transducer-array element weights at reception, referred to here as receive compounding. An effect of such compounding is reduced spatial resolution, causing smearing of point-like image structures, filling of cysts, and expansion of hyperechoic regions. In this article, we study how these unwanted effects can be mitigated by combining the compounding with a small, phase-based, adaptive steering of the array at reception. The adaptivity is based on a criterion akin to that of the Capon beamformer; a minimum-output distortionless response. Here, the distortionless part ensures that however we steer, we have a uniform at-focus response. We have applied this adaptive steering in combination with several receive compounding techniques on simulated Field II, phantom, and in vivo data. The results show that all the studied compounding techniques respond to this positively in light of the mentioned unwanted effects. The technique based on Thomson's multitaper method even surpassed the noncompounded equivalent in reproducing the geometry of structures. The speckle reduction, as measured by the change in the pixel mean to standard deviation ratio, is indeed lower, and there are subtle changes in the spatial speckle patterns when applying steering; however, we believe that in most cases, the negative effects are tolerable in light of the benefits gained. The suggested approach is intuitive and easily implemented.

Unsupervised Ship Detection for Single-Channel SAR Images Based on Multiscale Saliency and Complex Signal Kurtosis

Traditional ship detection methods for synthetic aperture radar (SAR) mainly utilize the amplitude information to distinguish ship targets from sea clutter, including constant false alarm rate (CFAR), visual attention model, and deep learning methods. The CFAR algorithms adopt the dense sliding window strategy, which is very time-consuming and may generate numerous false alarms. The deep learning methods are supervised and difficult to obtain satisfactory performance when the number of labeled samples is insufficient. The visual attention models can quickly focus on the potential target area, and however, it is still difficult to eliminate the strong clutter, such as radio frequency interference and azimuth ambiguity. In fact, as a coherent imaging system, SAR data itself are complex-valued. Compared with the amplitude information, complex information can essentially reflect the difference between ship target and sea clutter. To improve the accuracy and efficiency of ship detection, in this letter, a novel unsupervised ship detection method based on multiscale saliency and complex signal kurtosis (MSS-CSK) for single-channel SAR images is proposed, which contains the proposal extraction stage and the target discrimination stage. The experimental results based on the Radarsat-2 real SAR data show that the proposed method has high detection accuracy and efficiency.

Subtle changes of the crystalline lens after cycloplegia: a retrospective study

BackgroundThe purpose of this study was to evaluate the shape of the crystalline lens in terms of biometry and diopters before and after cycloplegia using the CASIA2 swept-source (SS) optical coherence tomography (OCT) system on the anterior segment.MethodsThis was a retrospective study. Children and adolescents (26 males and 29 females, aged 4–21 years) with simple ametropia were selected for optometry and CASIA2 imaging at 2 separate visits before and after cycloplegia. Diopter values were derived from the spherical power (S) obtained by optometry. Biometric parameters of the crystalline lens, including the anterior chamber depth (ACD), anterior and posterior curvature of the lens (ACL and PCL), lens thickness (LTH), lens decentration (LD), lens tilt (LT), and equivalent diameter of the lens (LED), were measured by the CASIA2 system. The differences in these parameters after compared with before cycloplegia were determined, and their relationships were analyzed.ResultsFifty-five participants (106 eyes) were initially enrolled. There was a significant difference (P < 0.05) in the S (t=-7.026, P < 0.001), ACD (t=-8.796, P < 0.001), ACL (t=-13.263, P < 0.001) and LTH (t = 7.363, P < 0.001) after compared with before cycloplegia. The change in the PCL (t = 1.557, P = 0.122), LD (t = 0.876, P = 0.383), LT (t = 0.440, P = 0.661) and LED (t=-0.351, P = 0.726) was not statistically significant (P > 0.05). There was a significant (P < 0.05) correlation of the change in the S with that in the ACL (r = 0.466, P < 0.001), LTH (r=-0.592, P < 0.001), and LED (r = 0.223, P = 0.021) but not the PCL (r = 0.19, P = 0.051), LD (r=-0.048, P = 0.0628) or LT (r=-0.022, P = 0.822). Furthermore, the change in the ACD was closely related to the change in crystalline morphology. However, in children and adolescents, we found that the change in crystalline morphology was unrelated to age.ConclusionsChanges in lens morphology after compared with before cycloplegia are mainly related to the ACL and LTH, but there is no difference in the PCL, LD, LT, or LED. In the adolescent population, change in the S is related to change in the ACL, LED and LTH. However, age is unrelated to the shape and tendency of the crystalline lens. Further research is required to determine whether the same conclusion applies to different age groups and different refractive states (myopia, hyperopia, emmetropia) .

Indentation probe with optical fibre array‐based optical coherence tomography for material deformation

We present a new optomechanical probe for mechanical testing of soft matter. The probe consists of a micromachined cantilever equipped with an indenting sphere, and an array of 16 single‐mode optical fibres, which are connected to an optical coherence tomography (OCT) system that allows subsurface analysis of the sample during the indentation stroke. To test our device and its capability, we performed indentation on a PDMS‐based phantom. Our findings demonstrate that Common Path (CP)‐OCT via lensed optical fibres can be successfully combined with a microindentation sensor to visualise the phantom's deformation profile at different indentation depths and locations in real time.Lay DescriptionThis work presents a new approach to simultaneously perform micro‐indentation experiments and OCT imaging. An optical fiber array‐based sensor is used to develop a new hybrid tool where micro‐indentation is combined with optical coherence tomography. The sensor is therefore capable of compressing a sample with a small force and simultaneously collecting OCT depth profiles underneath and around the indentation point. This method offers the opportunity to characterize the mechanical properties of soft materials and simultaneously visualize their deformation profile. The ability to integrate OCT imaging with indentation technology is promising for the non‐invasive and precise characterization of different soft materials.