CO2 Extraction Research Articles

Chemical composition, antioxidant, and antimicrobial activities of P. roxburghii oleoresin essential oils extracted by steam distillation, superheated steam, and supercritical fluid CO2 extraction.

Pinus roxburghii is a rich source of high-quality oleoresin that is composed of resin acids and essential oil (EO). The present research work was planned to study and compare the yield, biological activities, and chemical profiling of P. roxburghii oleoresin EOs extracted through various green extraction methods. Steam distillation (SD), supercritical fluid extraction, and superheated SD (SHSD) at different temperatures (120, 140, and 160°C) were employed to extract EOs from P. roxburghii oleoresin. Antioxidant potential of EOs was determined by total antioxidant content/ferric-reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH)-free radical scavenging activity (DPPH-FRSA), hydrogen peroxide scavenging assays, and percentage inhibition in linoleic acid. Antimicrobial activity of EOs was determined by resazurin microtiter-plate, disc diffusion, and micro-dilution broth susceptibility assays. Gas chromatography-mass spectrometry was used to determine the chemical composition of EOs. It was observed that extraction methods significantly affected the yield, biological activities, and chemical composition of EOs. The maximum yield (19.92%) was found in EO extracted by SHSD at 160°C. EO extracted by SHSD at 120°C showed the highest DPPH-FRSA (63.33%±0.47%), linoleic acid oxidation inhibition (96.55%±1.71%), hydrogen peroxide scavenging activity (59.42%±0.32%), and total antioxidant contents/FRAP (134.49%±1.34mg/L of gallic acid equivalent). The antimicrobial activity results showed that superheated steam-extracted EO of 120°C revealed the highest antifungal and antibacterial activity. It is concluded that SHSD is an alternative and effective technique for the extraction of oleoresins EO that improves the EO yield and biological activities. Further research on optimization and experimental parameters for the extraction of P. roxburghii oleoresin EO by SHSD is required.

A Technological Review of Direct Air Carbon Capture and Storage (DACCS): Global Standing and Potential Application in Australia

There is mounting evidence that, unless greenhouse gas (GHG) emissions fall back quickly, the goals outlined by the 2015 Paris Agreement to keep the global temperature rise well below 2 °C and preferably 1.5 °C will not be met. In response to these concerns, direct air carbon capture and storage (DACCS) technologies are gaining research and development attention. This article provides a thorough comparison of the two leading DACCS variants and reports on their status among major research and policy institutions worldwide. By translating the operating and capital costs to the Australian context, we assess the viability of DACCS implementation using either cheap renewable or legacy fossil energy to power CO2 extraction plants.

Correlation analysis of injection parameters for low-medium pressure injection of liquid CO2 for CH4 displacement in coal seams

The injection parameters for liquid CO2 to displace CH4 in a coal seam depend on engineering analogy and construction experience. To explore the relationship between key pressure injection parameters, such as liquid injection pressure and liquid injection volume, a combination of theoretical analysis and a downhole pilot test was used. The effective displacement radii of the CO2 and CH4 extraction under different liquid injection pressures were compared. The correlations between the volume, flow, time, and pressure of the injection liquid were analyzed. The results showed that the influence radius of CO2 effective displacement and influence period of CO2 pressure injection were proportional to the injection pressure. Low-pressure tank injection effectively promoted the extraction of coal seam CH4, whereas medium-pressure pump injection greatly enhanced the extraction of CH4. Therefore, in a low-to-medium pressure injection range, the higher the injection pressure of the liquid CO2, the better the displacement effect. A correlation analysis of the liquid injection parameters reveals that the liquid injection volume shows a concave nonlinear increasing trend with an increase in the liquid injection pressure, the liquid injection flow shows a parabolic trend of the upper opening with the increase of the liquid injection pressureand the liquid injection time shows a parabolic trend of the lower opening with an increase in liquid injection pressure. A comprehensive analysis shows that the optimal injection pressure and volume for the low-medium pressure injection of liquid CO2 to displace coal seam CH4 are 7.5 MPa and 7.4 m3, respectively.

Numerical Simulation of CO2 Extraction from the Cement Pre-Calciner Kiln System

The cement industry is one of the primary sources producing anthropogenic CO2 emissions. The significant increase in the demand for cement in years has significantly contributed to the increase in carbon emissions. Among numerous CO2 treatment technologies, calcium looping (CaL) is a practical approach to mitigating CO2 emissions. This paper used calcium looping (CaL) to capture CO2 from flue gas in a cement pre-calciner kiln system. The raw material exiting the lowest stage of the preheater is used as a calcium-based adsorbent, and the carbonation reactor is built between the tertiary and secondary preheaters, using the high-temperature flue gas exiting the tertiary preheater to provide heat for the reaction. The CFD (Computational Fluid Dynamics) simulation technology was used to evaluate the rationality of the carbonation reactor and the key factors affecting the carbon removal efficiency of the carbonation reactor. The results indicate that the velocity and pressure fields of the carbonation reactor conform to the general operating rules and are reasonable. The optimal operating speed of particles in the carbonation reactor is 15 m/s, with a separation efficiency of particles of 92.5%, ensuring the smooth discharge of reaction products. The factor analysis of the carbonation reactor shows that when the temperature is 911 K, the mass flow rate of CaO is 2.07 kg/s, and the volume fraction of CO2 is 0.28, the carbonation reaction reaches a chemical equilibrium state, and the carbon removal efficiency is 90%. It should be noted that this carbon removal efficiency is the optimal carbon removal efficiency based on a combination of economic factors. In addition, the influencing factors show a precise sequence: CO2 volume fraction > CaO addition amount > temperature. Finally, we investigated the impact of the addition of the carbonation reactor on the preheater system. The results show that adding the carbonation reactor causes an increase in the flue gas velocity at the outlet of the preheater and a decrease in pressure, reducing the separation efficiency. Although the separation efficiency decreases slightly, the impact on the pre-calciner system is minimal.

Comparative Analysis of the CO2 Extraction from Biogas Absorption Processes Effectiveness

Currently, the developed EU countries have implemented biomethane production projects from biogas, supply it to natural gas distribution grid with subsequent production of electricity or (and) heat, and use biomethane as motor fuel or supply to the gas network. It is also extremely relevant for Ukraine, supposing the problems with gas import due to Russian aggression and the presence of a large agricultural potential. The concern that arises is the rational choice of the technology for producing biomethane from biogas.The Gas Institute of the National Academy of Sciences of Ukraine has extensive experience in the development of technologies for the biogas collection, its direct usage, and CO2 extraction by the amine absorption method. Some of the technologies have been implemented at landfills in Ukraine. Data on other methods of CO2 extraction are widely available in world publications, so the authors compared the technologies from the point of view of their practical use possibility. Using computer modeling, the energy costs during the production of biomethane from biogas using the most advanced amine and water absorption processes for cleaning biogas from carbon dioxide were analysed. The combined water-amine absorption method of biogas purification from CO2 was included in the comparative analysis in which carbon dioxide was previously removed by water absorption at a pressure up to 0.3 MPa and output finally purified by amine absorption. Calculations for amine technology are verified in a pilot study.For a range of the CO2 concentration in biogas 32– 42 % vol., the specific energy consumption when using water absorption, the extraction of carbon dioxide from biogas is on average two times less compared to amine absorption, but at the same time the loss of CH4 due to its solubility during water absorption amounted to 7.1–7.6 %, with practically no losses of CH4 in amine absorption, and minor losses (0.17–2.8 %) in combined water-amine technology.The energy consumption of combined water-amine absorption is comparable to that of water absorption due to: a) reduction of heat losses for regeneration process of saturated amine absorbent, as part of CO2 has already been removed with water technology; b) using the CH4 excess to compensate power consumption of the biogas compressor during the preliminary water absorption of CO2 and/ or to compensate heat costs of the saturated amine absorbent regeneration.The results of extracting carbon dioxide from biogas processes modeling can be used to optimize technological absorption schemes for the production of biomethane, an analogue of natural gas.

Extraction of Apple Pomace from Juice Production Using Supercritical CO2 Extraction

Apple pomace, a by-product of apple juice and cider production, is a sustainable raw material from which valuable products such as nutritional supplements and pectin can be obtained. It contains significant amounts of antioxidant compounds that have been linked to several health benefits. Both traditional and new technologies can be used to extract valuable components from apple pomace, with an emphasis on new and environmentally friendly methods. One such technique is the use of supercritical CO2 extraction. This method is considered environmentally friendly, and it can be used to extract valuable compounds such as antioxidants and pectin from apple pomace. This article examines the extraction parameters of apple pomace and analyses the valuable substances in the extract samples. Apple pomace is a promising source of carbohydrates, proteins, amino acids, fatty acids, phenolic compounds, vitamins, and other compounds with a vast range of food applications.

Experimental study on microstructural modification of coal by liquid CO2 extraction

Liquid CO2 injection into coal seam for CH4 displacement is one of the important means to strengthen gas extraction and carbon sequestration in recent years. After liquid CO2 is injected into coal seam, physical and chemical interactions occur with coal through the process of “liquid phase seepage-phase transformation supercharging-gas–liquid mass transfer”, and microscopic characteristics of coal such as pore distribution, surface chemical properties and adsorption characteristics change. Three kinds of coal samples with different degrees of metamorphism were selected as research objects, theoretical analysis, numerical simulation and experimental testing were combined to carry out research on the modification of coal body by liquid CO2 extraction. The results show that with the increase of extraction pressure, the diffusion rate of small organic molecules in coal increases, which is conducive to the dissolution of small organic molecules; In larger pores, the contact area between liquid CO2 and pore surface increases, and small molecular organic matter is easy to precipitate; The infrared spectra of raw coal and residual coal after extraction are basically the same, and the absorption peak intensity is significantly different, liquid CO2 extraction does not significantly damage the macromolecular structure of coal. The research results have important reference value for liquid CO2 anti-reflection coal seam and geological storage.

From Coffee Waste to Active Ingredient for Cosmetic Applications.

Coffee silverskin (CS) is the thin epidermis covering and protecting the coffee bean and it represents the main by-product of the coffee roasting process. CS has recently gained attention due to its high content in bioactive molecules and the growing interest in valuable reutilization of waste products. Drawing inspiration from its biological function, here its potential in cosmetic applications was investigated. CS was recovered from one of the largest coffee roasters located in Switzerland and processed through supercritical CO2 extraction, thereby generating coffee silverskin extract. Chemical profiling of this extract revealed the presence of potent molecules, among which cafestol and kahweol fatty acid esters, as well as acylglycerols, β-sitosterol and caffeine. The CS extract was then dissolved in organic shea butter, yielding the cosmetic active ingredient SLVR'Coffee™. In vitro gene expression studies performed on keratinocytes showed an upregulation of genes involved in oxidative stress responses and skin-barrier functionality upon treatment with the coffee silverskin extract. In vivo, our active protected the skin against Sodium Lauryl Sulfate (SLS)-induced irritation and accelerated its recovery. Furthermore, this active extract improved measured as well as perceived skin hydration in female volunteers, making it an innovative, bioinspired ingredient that comforts the skin and benefits the environment.

Fractionation of Marigold Waxy Extract Using Supercritical CO2

Marigold oil is a product of great industrial interest thanks to its wide range of medicinal and wound-healing properties. In this work, supercritical carbon dioxide was used to recover marigold essential oil from the hexane solvent extract of marigold flowers, the floral “concrete”. This starting material was mixed with synthetic paraffinic waxes to heighten its melting point and viscosity, thus, improving material processability. Supercritical fluid extraction and fractionation of the modified marigold “concrete” was carried out, and the effect of pressure and CO2 mass flow rate was studied. The pressure was varied from 80 to 180 bar, keeping the temperature constant at 40 °C: the higher the pressure, the larger the CO2 solvent power and extraction yield (up to 9.40% w/w). Nevertheless, the optimum between productivity and process selectivity was found at 100 bar. By changing the CO2 mass flow rate (from 1.20 to 1.50 kg/h), we noted that mass transfer resistance was located externally. GC-MS analysis showed that the most abundant compounds in the oil were δ-cadinene (25%), γ-cadinene (16%), τ-muurolol (6.5%), and α-muurolene (6%). Moreover, the traces of oil and waxes showed no mutual contamination between lighter species and waxes, meaning that the fractionation step was successful.

Influence of Liquid CO2 Extraction and Dissolution on Coal Adsorption Characteristics

Liquid CO2 is a non-polar fluid, and the injection of CO2 fluid into a coal seam causes a strong water–rock interaction between the inorganic minerals and organic matter in the coal and acidic fluid. The minerals in the coal are thereby corroded and precipitated to different degrees, and the organic matter is dissolved and extracted, which further changes the physical and chemical properties of the coal and rock. Three kinds of coal samples with different metamorphic degrees were selected as the research objects, and the research methods of theoretical analysis and experimental testing were used to carry out the related research on the modification of coal by liquid CO2 extraction and dissolution. After the three kinds of coal samples were extracted by liquid CO2, the pore specific surface area decreased and the CO2 adsorption decreased with the increase in extraction pressure. The reduction in anthracite adsorption was greater than that of bituminous coal and lignite; after being corroded by different CO2 pressures, the adsorption capacity of bituminous coal gradually increased with the increase in corrosion pressure, and the increase in adsorption capacity of bituminous coal was larger than that of anthracite and lignite. After corrosion, bituminous coal was suitable for CO2 geological storage.

Insights into the Oxidative Degradation Mechanism of Solid Amine Sorbents for CO2 Capture from Air: Roles of Atmospheric Water

AbstractDirect air capture (DAC) processes for extraction of CO2 from ambient air are unique among chemical processes in that they operate outdoors with minimal feed pretreatments. Here, the impact of humidity on the oxidative degradation of a prototypical solid supported amine sorbent, poly(ethylenimine) (PEI) supported on Al2O3, is explored in detail. By combining CO2 adsorption measurements, oxidative degradation rates, elemental analyses, solid‐state NMR and in situ IR spectroscopic analysis in conjunction with 18O labeling of water, a comprehensive picture of sorbent oxidation is achieved under accelerated conditions. We demonstrated that the presence of water vapor can play an important role in accelerating the degradation reactions. From the study we inferred the identity and kinetics of formation of the major oxidative products, and the role(s) of humidity. Our data are consistent with a radical mediated autooxidative degradation mechanism.

Insights into the Oxidative Degradation Mechanism of Solid Amine Sorbents for CO2 Capture from Air: Roles of Atmospheric Water.

Direct air capture (DAC) processes for extraction of CO2 from ambient air are unique among chemical processes in that they operate outdoors conditions with minimal feed pretreatments. Here, the impact of humidity on the oxidative degradation of a prototypical solid supported amine sorbent, poly(ethylenimine) (PEI) supported on Al2O3, is explored in detail. By combining CO2 adsorption measurements, oxidative degradation rates, elemental analyses, solid-state NMR and in situ IR spectroscopic analysis in conjunction with 18O labeling of water, a comprehensive picture of sorbent oxidation is achieved under accelerated conditions. We demonstrated that the presence of water vapor can play an important role in accelerating the degradation reactions. From the study we inferred the identity and kinetics of formation of the major oxidative products, and the role(s) of humidity. Our data are consistent with a radical mediated autooxidative degradation mechanism.

Investigation of Conversion of Sunflower Oil Production Wastes to High Value Compounds by Supercritical CO2

The evaluation of wastes from edible oil production industry has increasing importance because of resources deficiency and growing population day by day. It was aimed to investigate the recovery potential of oil and valuable components such as total phenolic contents (TPC), total flavonoid content (TFC) and antioxidant activities with conventional and green extraction methods from edible sunflower oil production wastes. Firstly, Soxhlet extraction was applied on the waste samples from filtration of the oil and then supercritical CO2 (SC- CO2) extraction was performed to compare extraction yield. The statistical evaluation of the SC- CO2 extraction experiments was conducted with the Minitab Software program. It was concluded that 37% extraction yield was obtained with the mixture of perlite and waste sample, while the highest recovery was observed as 27% for the perlite-free sample. The interaction of independent variables (temperature and pressure) on the recovery yields were determined as significant (p=0.002). Pressure was detected more effective on the valuable substance contents of the extracts than temperature. The most efficient conditions were 50 oC and 22.1 MPa for oil recovery, while 60 oC and 20 MPa for valuable substance recovery.

Safety of yellow/orange tomato extract as a novel food pursuant to Regulation (EU) 2015/2283.

Following a request from the European Commission, the EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA) was asked to deliver an opinion on yellow/orange tomato extract used as a novel food (NF) pursuant to Regulation (EU) 2283/2015. The NF which is the subject of the application is a carotenoid-rich extract from the yellow/orange tomato containing predominantly phytoene and phytofluene, as well as a lesser amount of beta-carotene, zeta-carotene and lycopene. The NF is produced from the tomato pulp using supercritical CO2 extraction. The applicant proposes the use of the NF in cereal bars, functional drinks and as a food supplement in individuals above 15 years of age. For the use of the NF in cereal bars and functional drinks, the Panel considers, the target population is the general population. According to EFSA's latest exposure assessment for lycopene as a food additive (EFSA ANS Panel, 2017), the highest P95 intakes for children (< 10 and 10-17 years) and adults when combined to the use of lycopene as a food colour from natural occurrence would exceed the established acceptable daily intake (ADI) for lycopene (0.5 mg/kg body weight (bw) day). The estimated intakes of the NF would lead to an exceedance of the ADI when considering natural occurrence and exposure to lycopene when used as a food additive. Due to the absence of safety data regarding phytoene and phytofluene intake from the NF, and the contribution of the NF to the estimated high daily intakes of lycopene, the Panel considers that it cannot be established whether or not the consumption of the NF is nutritionally disadvantageous. The Panel concludes that the safety of the NF has not been established under the proposed conditions of use.

Component composition and antimicrobial activity of subcritical CO2 extract of Ferula asafoetida L., growing in the territory of Kazakhstan

Ferula asafoetida L. has been studied for centuries by many scientists as food products and in traditional medicine, which are relevant for Kazakhstan as well. Recent studies have shown that Ferula asafoetida L. has neuroprotective, memory-enhancing, digestive enzymatic, antioxidant, antispasmodic, hypotensive, hepatoprotective, antimicrobial, antitumor, cytotoxic, and anthelmintic effects. The aim of this study is to determine the component composition by GC-MS method and to study the antimicrobial properties of Ferula asafoetida L. extract obtained by CO2 extraction, which grows in Kazakhstan. Materials and methods. To determine the possibility of using Ferula asafoetida L., we carried out the component composition of the extract obtained by CO2 extraction in subcritical conditions of the underground part of Ferula asafoetida L. by a certain GC-MS method and also investigated the antimicrobial effect of this extract. Results. The plant raw materials were collected in accordance with GACP requirements. Conducted subcritical CO2 extraction of plant raw materials showed a 2.5% extraction yield. The study of the component composition by GC-MS revealed 46.3% of sulfate compounds. The determination of antimicrobial activities showed high efficacy against gram-positive (Staphylococcus aureus subsp. Aureus, Bacillus subtilis subsp. Spizizenii), gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae subsp. pneumoniae, Salmonella enterica subsp. enterica) and fungi (Candida albicans, Aspergillus niger). Conclusions. The possibility of using the obtained CO2 extract of Ferula asafoetida L. in the field of pharmaceutical products as a substance and a drug that has a huge antimicrobial effect

Simulation of a flexible multi-vessel extraction plant with counter-current contacting between a solid substrate and supercritical CO2

This work develops a program (MVSEPS) to simulate a multi-vessel SuperCritical Fluid Extraction (SCFE) plant operating in a counter-current fashion. MVSEPS was applied to an SCFE to extract hemp seed oil using the shrinking core mass transfer model. A microstructural factor was best-fitted to experimental data from literature, to describe the inner mass transfer of the oil within the substrate, that was used as a parameter in subsequent simulations. The oil concentration in the CO2 stream exiting an extraction vessel, and the productivity, and efficiency of the SCFE plant were studied by changing selected parameters of the process and extraction conditions. The number of extraction vessels operating in series and the cycle time were relevant simulation parameters, because they cannot be studied experimentally at a laboratory scale. Using a larger number of extraction vessels and shorter cycle times increase the plant productivity but decrease the extraction efficiency. Other parameters studied, such as the particle size of the milled substrate, superficial CO2 velocity, extraction temperature and pressure, impact the operation of the SCFE plant similarly to at laboratory scales.

Determination of Chemical Composition and Antimicrobial Activity of the CO2 Extract of Eryngium planum L.

The article presents parameters for obtaining a carbon dioxide extract from the subterranean part of Eryngium planum that contains a valuable set of organic substances and has a certain antimicrobial effect. Methods. Raw materials were collected in the Almaty region (Republic of Kazakhstan). The CO2 extract of Eryngium planum herbs was obtained under subcritical conditions. A gas chromatograph with a mass spectrometric detector was used to determine the compositional breakdown of the extract. Antimicrobial activity was determined by two methods: the micromethod of serial dilutions and the disk-diffusion method. Three microbial test strains were used: Staphylococcus aureus ATCC 6538-P, Escherichia coli ATCC 8739, and Candida albicans ATCC 10231. Results. To extract biologically active substances from the subterranean part of Eryngium planum L., we have chosen carbon dioxide extraction technology, a technology for processing carbon dioxide (CO2) raw materials, which allows us to extract various substances in high concentrations. Carbon dioxide extraction technology is an effective and environmentally safe way to isolate various biologically active substances contained in medicinal plant raw materials. In the composition of the CO2 extract of Eryngium planum L. 43 components were identified, the main of which are α-linolenic acid, 8.30%; myristic acid, 6.40%; caryophyllene, 6.92%; spatulous, 6.62%; and other main identified compounds and their percentage. Conclusions. The study showed that the CO2 extract of Eryngium planum L. contains biologically active compounds that have a pronounced antimicrobial effect against clinically significant microorganisms, such as Escherichia coli, Staphylococcus aureus, and Candida albicans.

Comparative Study of E-Nose, GC-MS, and GC-IMS to Distinguish Star Anise Essential Oil Extracted Using Different Extraction Methods

In this study, star anise (Illicium verum) essential oils (SAEOs) were extracted by hydrodistillation (HD), ethanol solvent extraction (ESE), supercritical CO2 (SCD) and subcritical extraction (SE) via electronic nose (E-nose), gas chromatography-mass spectrometry (GC-MS), and GC-ion mobility spectrometry (GC-IMS). GC-MS and GC-IMS were used to identify the volatile compounds, and GC-MS was also used to determine their concentrations. Principal component analysis (PCA) and linear discriminant analysis (LDA) were used to visualise volatile compounds and differentiate samples. The results showed that anethole and limonene were the main volatile compounds in SAEOs extracted using the four methods and their components were similar, albeit in different proportions. In addition, the fingerprints of their volatile components were established via E-nose and GC-IMS analyses. In general, GC-MS, GC-IMS, and E-nose combined with PCA and LDA analysis could accurately distinguish SAEOs extracted using different extraction methods, and GC-IMS was identified as the most suitable method because of its accuracy and rapidity.

Evaluation of the biological activity and chemical profile of supercritical and subcritical extracts of Bursera graveolens from northern Peru

Bursera graveolens is a tree species located in regions between Mexico and Peru. It has been used in folk medicine, but no studies have reported on obtaining extracts of B. graveolens at high pressure. Therefore, this study compared low and high pressure extraction methods, using supercritical extraction with CO2 and subcritical extraction using CO2-EtOH, to evaluate the biological properties and chemical profile of extracts. Global yield, cytotoxic activity, antimicrobial activity and chemical profile using GC/MS were evaluated. The best global yields were obtained under subcritical conditions with %EtOH greater than 20%. Extracts from supercritical conditions showed cytotoxicity to L929 cells from 50 µg mL−1 and antimicrobial effect against dermatophyte fungi from 500 µg mL−1. Chemical profile analysis showed recovery of 17.50% of d-limonene, 28.30% of mint furanone, and 12.36% of α-terpineol in subcritical extracts. Since these compounds are an alternative to synthetic insecticides, they would have industrial value.

Essential Oils of Ginkgo biloba Yellow Leaves Obtained by Hydrodistillation and Supercritical Carbon Dioxide Extraction

Background/Objective: Volatile compounds present in green Ginkgo biloba L. leaves extracts contain compounds that can undertake free radical scavenging. The assumption was that volatile compounds present in yellow Ginkgo biloba leaves extracts have a different composition compared to green Ginkgo biloba leaves extracts. The investigation was conducted in order to determine the compounds in yellow Ginkgo biloba leaves’ extracts that can undertake free radical scavenging. Methods: The volatile compounds present in yellow Ginkgo biloba leaf extracts were obtained by hydrodistillation and supercritical carbon dioxide (CO2) extraction. The obtained essential oils were analyzed by gas chromatography–mass spectrometry. Results: The essential oil obtained by hydrodistillation consisted of monoterpenes (3.1%), oxygenated monoterpenes (15.3%), sesquiterpenes (6.2%), oxygenated sesquiterpenes (51.4%), esters (1.3%), benzopyrans (7.1%), an oxolane (1.5%), and a rose ketone (0.8%). Caryophylene-4(12),8(13)-dien-5α-ol (7.1%) was the most abundant compound, followed by 14-hydroxy-( Z)-caryophyllene (5.8%), caryophyllene oxide (5.8%), and hydroxydihydroedulan (5.6%). The supercritical fluid extraction was done under the pressure of 30 MPa, at the temperature of 40 °C and the CO2 flow rate was 2 kg·h−1. The obtained chromatogram revealed the presence of 12 compounds. The main constituents were squalene (110.5 mg per 100 g of plant material) and α-tocopherol (99.3 mg per 100 g of plant material). Conclusion: The well-known compound that has a free radical scavenging activity α-tocopherol was determined in yellow Ginkgo biloba leaves extract obtained by supercritical CO2 extraction.