Clerodendrum Inerme Research Articles

A virus inhibitory protein isolated from Cyamopsis tetragonoloba (L.) Taub. upon induction of systemic antiviral resistance shares partial amino acid sequence homology with a lectin.

Two virus inhibitory proteins were purified from Cyamopsis tetragonoloba , induced to resist virus infections by CIP-29, a systemic resistance inducing protein from Clerodendrum inerme, and characterized. One of them shared homology with a lectin. CIP-29, a known 29 kDa systemic antiviral resistance inducing protein isolated from Clerodendrum inerme, has been used to induce systemic resistance in Cyamopsis tetragonoloba against Sunn-hemp rosette virus (SRV). Paper reports the detection of virus inhibitory activity in induced-resistant leaf sap of C. tetragonoloba, and the purification of two virus inhibitory agents (VIAs) thereof. VIA activity was recorded as a reduction in lesion number of SRV, Tobacco mosaic virus, and Papaya ringspot virus, when they were incubated separately with resistant sap and inoculated onto susceptible C. tetragonoloba, Nicotiana tabacum cv. Xanthi-nc, and Chenopodium quinoa, respectively. The two VIAs were isolated from resistant C. tetragonoloba plant leaves using combinations of column chromatography. Both were basic proteins, and since their M r was 32 and 62 kDa, these VIAs were called CT-VIA-32 and CT-VIA-62, respectively, on the basis of their molecular mass and the host. CT-VIA-62 displayed better activity, and was thus studied further. It tested positive for a glycoprotein, and was serologically detected only in leaf tissue post-induction. Tryptic peptides generated in-gel, post SDS-PAGE of CT-VIA-62, were sequenced through LC/MS/MS. All CT-VIA-62 peptides were found to share homologies with proteins from Medicago truncatula that possess a mannose-binding lectin domain.

Acacetin Inhibits Glutamate Release and Prevents Kainic Acid-Induced Neurotoxicity in Rats

An excessive release of glutamate is considered to be a molecular mechanism associated with several neurological diseases that causes neuronal damage. Therefore, searching for compounds that reduce glutamate neurotoxicity is necessary. In this study, the possibility that the natural flavone acacetin derived from the traditional Chinese medicine Clerodendrum inerme (L.) Gaertn is a neuroprotective agent was investigated. The effect of acacetin on endogenous glutamate release in rat hippocampal nerve terminals (synaptosomes) was also investigated. The results indicated that acacetin inhibited depolarization-evoked glutamate release and cytosolic free Ca2+ concentration ([Ca2+]C) in the hippocampal nerve terminals. However, acacetin did not alter synaptosomal membrane potential. Furthermore, the inhibitory effect of acacetin on evoked glutamate release was prevented by the Cav2.2 (N-type) and Cav2.1 (P/Q-type) channel blocker known as ω-conotoxin MVIIC. In a kainic acid (KA) rat model, an animal model used for excitotoxic neurodegeneration experiments, acacetin (10 or 50 mg/kg) was administrated intraperitoneally to the rats 30 min before the KA (15 mg/kg) intraperitoneal injection, and subsequently induced the attenuation of KA-induced neuronal cell death and microglia activation in the CA3 region of the hippocampus. The present study demonstrates that the natural compound, acacetin, inhibits glutamate release from hippocampal synaptosomes by attenuating voltage-dependent Ca2+ entry and effectively prevents KA-induced in vivo excitotoxicity. Collectively, these data suggest that acacetin has the therapeutic potential for treating neurological diseases associated with excitotoxicity.

Acute and sub-acute toxicity study of Clerodendrum inerme, Jasminum mesnyi Hance and Callistemon citrinus

ObjectiveTo study acute and sub-acute toxicity study of Clerodendrum inerme (C. inerme), Jasminum mesnyi (J. mesnyi) Hance and Callistemon citrinus (C. citrinus). MethodsThe acute toxicity test was conducted in Swiss albino mice. The extracts of C. inerme, J. mesnyi Hance and C. citrinus was administered in single dose of 0.5, 1.0, 2.0, 3.0, 4.0 and 5.0 g/kg and observed for behavioral changes and mortality, if any. In sub-acute toxicity study, Wistar rats of either sex were administered 1/5th of the maximum tolerated dose, p.o. for 4 weeks. Rats were observed weekly for any change in their body weight, food and water intake during 28 d of the treatment. At the end of 28 d, blood samples of the rats were collected for hematological and biochemical study. ResultsIn acute toxicity study, all four extracts of three plants were found to be well tolerated up to the dose of 2 000 mg/kg. These produced neither mortality nor any change in the behavior in mice. In sub-acute toxicity study, all four extracts of three plants at the LD50 dose level did not produce any significant alteration in hematological and biochemical parameters in rats. ConclusionsThe results demonstrated that there is a wide margin of safety for the therapeutic use of each of the four extracts of three plants. The findings also corroborated the traditional use of these extracts.

Post-tsunami natural regeneration of coastal vegetation in the Hambantota district in south-eastern Sri Lanka

A qualitative rapid survey was conducted in 45 plots distributed in the gentle seashore vegetation andsand dunes (n = 13), coastal scrublands (n = 19), and mangroves (n = 13) along the coastline ofHambantota District, affected by the Indian Ocean tsunami. The objective of the survey was toinvestigate the natural regeneration of coastal vegetation 20 months after the tsunami disturbance21 plant species belonging to 19 families were recorded as the prominent plants regenerating inaffected mangroves, while 16 species in 15 families and 32 species in 23 families were observed asprom inent plants to regenerate in affected areas of the gentle sea-shore vegetation and coastalscrublands respectively.In tsunami affected mangrove stands Acanthus iltctfolius (in 50% of study plots), Achrosticumaureum (40%) and Lumnitzera racemosa (17%) were the dominant species establishing in openmuddy substrates, while Clerodendrum inerme (57%), Lumnitzera racemosa and Excoecariaaga//ocha (29% each) were regenerating in sand deposited in the mangrove patches.Ipomoea pes-caprae (85%), Scaevola taccada and Calotropis gigantea (23% each) were observedas the dominant species re-establishing in the gentle seashore vegetation, while Spinifex littoreusshows a slow rate of regeneration. Most of the destroyed Pandanus odoratissimus bushes facingthe beach are not regenerating. Instead a new row of Pandanus was observed regenerating immediatelybacking the original stands. Prominent species regenerating in coastal scrublands are Crotonbonplandianus and Gymnema sylvestre (37% each), Clerodendrum inerme (16%), Calotropisgigantea (10%) and Crateva adansonii (10%) and saplings of Azadirachta indica and Limoniaacidissitna .Invasive alien plants, mainly Opuntia dillennii have established well and spreading vigorously inaffected coastal scrublands (58%), some study plots of gentle seashore vegetation (31 %) as wellas on sand depositions in the affected mangroves (15%). This species was observed replacing thespaces occupied by destroyed Pandanus odoratissimus bushes and Spinifex littoreus beds.Invasive alien plants such as Prosopis juliflora and Lantana camara were also spreading intsunami disturbed coastal scrublands.

PRELIMINARY PHYTOCHEMICAL ANALYSIS OF CLERODENDRUM INERME

The aim of the prese nt study was to determine phyto chemical analysis and the total alkaloid and flavonoid contents of the leaf part of Clerodendrum inerme plant . The general techniques of med icinal plant extraction are done by soxlet extraction from polar to non polar i.e. petroleum ether, chloroform, ethyl acetate, methano l & aqueous extraction. The ethyl acetate extract has been shown the presence of most of the active components. These extr acts were tested for alkaloids, terpenoids, flavonoids, Phenol, Steroids, Diterpene test, Flavonoids, Flavanones, Quinones, coumarins and Tannins. The total alkaloid and flavanoid content is 13.96% and 6.2% respectively . This proves its application in wide areas of medicine.

Two New Chalcones from the Flowers of Clerodendrum inerme

Two new chalcones, 3-hydroxy-3',4'-dimethoxychalcone (1) and 3,2'-dihydroxy-3',4'-dimethoxychalcone (2), were isolated from the flowers of Clerodendrum inerme (L.) Gaertn together with two known flavones, 7-O-methylwogonin (3) and eucalyptin (4). The structures of the new compounds 1 and 2 have been established by extensive 2D-NMR and ESI-TOFMS studies.

Comparative study of antimicrobial activities of some mangrove plants from Sundarban Estuarine Regions of India

The study investigated antimicrobial activities in different tissues of four mangrove plants:Avicennia marina (Forsk.) Vierh (Avicenniaceae), Avicennia alba Blume (Avicenniaceae),Clerodendrum inerme Gaertn (Verbenaceae) and Aegiceras corniculatum (L.) Blanco (Myrsinaceae) found in the Sundarban delta regions of West Bengal State, India. Organic solvent extracts obtained from different tissues of these plants were investigated by disc diffusion tests against Gram-positive bacteria (Bacillus subtilis, Bacillus coagulans), Gram-negative bacteria (Escherichia coli, Enterobacter sakazakii and Acinetobacter baumannii) and fungi (Saccharomyces cerevisiae and Termitomyces clypeatus). Results suggested that extracts from various parts of these plants had growth-inhibitory effects on fungus and Gram-positive bacteria. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) values were determined for some of these extracts. The antimicrobial activities were found to be heat stable when the crude extracts were subjected to controlled heat stress. The pH tolerance of the antimicrobial activities was found to be wide ranging with marginal changes in activities at extreme pH. The results suggested that these plants could be exploited in the management of various infectious diseases and their tissue extracts might have roles as pharmaceuticals and preservatives. Key words: Antimicrobial activity, mangrove, Sundarban Estuary, disc diffusion test, heat stability, pH sensitivity.

Study on the Optimization of Extraction Technology of Flavonoids in<i> Clerodendrum inerme</i>(L.) Gaertn. Roots

Abstract. Based on the results of single factor experiments on the extraction of total flavonoids content (TFC) in roots of semi-mangrove plant Clerodendrum inerme(L.) Gaertn., operation conditions were optimized with orthogonal methodology. Four factors were applied to evaluate the extraction yield, including the ethanol concentration, extraction time, temperature and solid to liquid ratio. According to the experimental results, the influence order of different factors on the yield of TFC from the roots: ethanol concentration > solid to liquid ratio > extraction temperature > extraction time. The optimum operating parameters were determined as following, ethanol concentration of 70.0 %, extraction time of 90.0 min, temperature of 60.0 °C and solid to liquid ratio of 1:30. Under the optimized conditions, the maximal yield of TFC was enhanced to 1.279%.

Volatile Constituents and Antioxidant Properties of the Ligarine Extracts of Twig, Leaf and Root of Clerodendrum Inerme

Volatile constituents were isolated from twig, leaf and root parts of Clerodendrum inerme by ligarine extraction and analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). There were 6, 12 and 10 volatile constituents identified from different parts, respectively. All the extracts have in common a great percentage of esters (28.59–69.76%) and a high amount of fatty acids (19.27–41.85%), with the exception of the extract from root, which consisted mainly of meroterpene (37.61%). The antioxidant activity by DPPH test was investigated for all the extracts. The root extract exhibited strong antioxidant property with IC50 value of 67.5 μg/ml, about 26% of the potency of known antioxidantα-tocopherol, which probably due to the presence of 6-methylene-3,3-diphenyl-1,4-cyclohexadiene (2.12%). And the characteristic odor of root extract could be attributed to the presence of ferruginol (37.61%) with strong sensory property. The results present here indicated a potential source suitable for using as antioxidant agent and offered theoretic basis for utilization of the traditional folk herb C. inerme.

Effect of medicinal and aromatic plants on rumen fermentation, protozoa population and methanogenesis in vitro

The potential of tannins from 21 medicinal and aromatic plant leaves as antimethanogenic additives in ruminant feeds was investigated. The effect of tannin from these leaves on rumen fermentation parameters, protozoa population and methanogenesis was studied by incubating the samples [200 mg dry matter (DM)] without and with polyethylene glycol (PEG)-6000 (400 mg DM) as a tannin binder during 24-h incubation in the in vitro Hohenheim gas method. Based on the methane percentage estimated in the total gas produced, methane production in millilitre was calculated [methane volume (ml) = methane % × total gas produced (ml) in 24 h]. In the samples, crude protein and neutral detergent fibre (g/kg DM) ranged from 113 to 172 and from 352 to 444 respectively. The total phenol (TP; g/kg DM) content was highest in Terminalia chebula (274) followed by Hemigraphis colorata (71) and Sapindus laurifolia (51) respectively. In the remaining samples, it was <43 g/kg DM. Activity of tannins, as represented by the increase in gas volume on addition of PEG, ranged from 0 to 133%, with the highest being recorded in T.chebula. The per cent increase in methane on PEG addition was 0 for Ammi majus, Aristolochia indica, Cascabela thevetia, Ipomea nil and Lantana camara, illustrating that tannins present in these samples had no effect on methane concentration. The PEG addition increased the total protozoa count by >50% in A.indica and C. thevetica. One of the important findings of our study was that of the 21 samples screened, Entodinia population increased in 12 with PEG as compared to 7 where Holotricha increased, indicating higher susceptibility of Entodinia to tannin. There was no increase in the protozoa population with PEG when incubating Cardiospermum halicacabum, Clerodendrum inerme, Dioscorea floribunda, Nerium oleander and Selastras paniculatus, which strongly suggested that methane suppression recorded in these samples was not because of a defaunating effect of their tannins per se. The fermentation pattern reflected increased total volatile fatty acid (TVFA) concentration from 0 to 28.3% with PEG addition among the leaves. Our results confirmed further observations that methanogenesis in vitro is not essentially related to density of protozoa population. Secondly, medicinal and aromatic plants such as C.inerme, Gymnema sylvestre and Sapindus laurifolia containing tannins appear to have a potential to suppress in vitro methanogenesis.

In vitro synthesis of antimicrobial silver nanoparticles by mangroves, saltmarshes and plants of coastal origin

A total of 13 leaf extracts of coastal plants were used for the synthesis of silver nanoparticles and to test the antimicrobial potential of nanoparticles. The highest amount of nanoparticles was obtained by Sesuvium portulacastrum followed by Prosopis chilensis, Hibiscus rosasinensis and Clerodendrum inerme. The synthesised nanoparticles were spherical with variable size ranging from 5 to 20 nm, as evident by transmission electron microscopy. The synthesis of silver nanoparticles was confirmed with X-ray diffraction spectrum. Fourier transform infrared spectroscopy identified the presence of amide I, II and III, aromatic rings, geminal methyls and ether linkages indicating the presence of biomolecules responsible for stabilisation of the silver nanoparticles. Protein bands with molecular weight in a range of 35 to 100 kDa were detected. The nanoparticles inhibited clinical strains of bacteria and fungi with more distinct antifungal activity, which was enhanced when polyvinyl alcohol was added as a stabilising agent.

Diversity and useful products in some Verbenaceous member of Melghat and Amravati regions, Maharashtra, India

Ingole SN. 2011. Diversity and useful products in some Verbenaceous member of Melghat and Amravati regions, Maharashtra, India. Biodiversitas 12: 146-163. Verbenaceae is a large family of very diverse habit. The present study deals with detailed characteristics, distribution and economically important products of some verbenaceous members of Melghat and Amravati regions. During the survey twenty members belonging to fourteen genera of Verbenaceae were collected. Some members occur abundantly either in wild or cultivated state like Lantana camara L. var. aculeata Mold., Lantana flava Medik., L. nivea Vent., Glandularia bipinnatifida (Schauer)Nutt., Duranta erecta L., Vitex negundo L., Volkameria inermis L., Clerodendrum phlomidis L. f., Clerodendrum splendens G. Don, Nyctanthes arbor-tristis L. etc. while Petrea volubilis L., Gmelina arborea Roxb., G. philippensis Cham., Stachytarpheta jamaicensis (L.) Vahl., S. mutabilis (Jacq.) Vahl., Rotheca serrata (L.) Steane &amp; Mabb., Holmskioldia sanguinea Retz. are not much common and occur in limited locations. Phyla nodiflora (L.) Greene, a creeping much-branched herb is found typically in wet places. Tectona grandis L. f. occurs very variable in size according to its habitat and is common dominant tree in forest of Melghat and also planted in plains. Clerodendrum infortunatum L., a gregarious tomentose shrub is exclusively found in shades of forest at limited spots in higher elevations of Melghat. The various members are not only beautiful ornamentals but also the source of important medicinal products useful in a broad range of diseases including skin disorders and snake remedies; they contain alkaloids, sterols, saponin, glucosides, dyes etc. and are economically quite important e.g. as high quality timber. On basis of morphological diversity the generic key is provided.

GRAIN PROTECTANT EFFICACY OF CERTAIN PLANT EXTRACTS AGAINST RICE WEEVIL, SITOPHILUS ORYZAE L. (COLEOPTERA: CURCULIONIDAE)

ABSTRACT In the present study, ethanol extract of Clerodendrum inerme L. (Verbenaceae), Withania somnifera L. (Solanaceae), Gliricidia sepia L. (Fabaceae), Cassia tora L. (Caesalpiniaceae) and Eupatorium odoratum L. (Asteraceae) were evaluated for their efficacy on mortality and progeny production of rice weevil, Sitophilus oryzae L. (Coleoptera: Curculionidae). Adult insects were exposed to the 2.5 and 5% extracts treated wheat and mortality was assessed after 1, 2, 7, 14 and 21 days. Subsequently, all adults were removed and the treated grains remained at the same conditions for an additional 45 days. After this interval, the commodity was checked for progeny production. All extracts, the beetles mortality was increased in dose dependent manner. Results indicated that C. inerme and W. somnifera extracts were more effective than G. sepia, C. tora and E. odoratum against adult insects. Interestingly, the progeny production (Fl) was complete by suppressed even in lowest dose. It was concluded that both C. inerme and W. somnifera can be used for the protection of stored wheat from infestations of S. oryzae.

A novel lupene-type triterpenic glucoside from the leaves of Clerodendrum inerme

A new triterpenic glucoside, lup-1,5,20(29)-trien-3-O-β-D-glucopyranoside (4), along with three known phytoconstituents: n-octacosane, friedelin and β-amyrin, has been isolated from the leaves of Clerodendrum inerme (L.) Gaertn. (Verbenaceae). Structure elucidation was carried out on the basis of chemical and physical evidence (IR, 1H-NMR, 13C-NMR, DEPT and MS spectra). The alcoholic and aqueous extracts of the leaves of C. inerme showed significant antinociceptive activity in analgaesiometer tests.

Hepatoprotective Effect of Clerodendrum inerme Linn. Ethanolic Extract

Twenty four rats were divided into four equal groups. The control group received distilled water intraperitoneally. The second group received carbon tetrachloride 0.5 ml/kg for seven days while the third group was injected with carbon tetrachloride 0.5 ml/kg and simultaneously administered with Clerodendrum inerme extract 200 mg/kg orally for seven days. The fourth group received carbon tetrachloride 0.5 ml/kg intraperitoneally together with silymarin 200 mg/kg orally for seven days. At the end of treatment blood samples collected by direct cardiac puncture and serum were used for the marker enzymes aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, as well as cholesterol and triglyceride content. Rats treated with the ethanolic extract of C. inerme showed a significant decrease in the levels of the markers. The results obtained indicate that C. inerme Linn. protects the liver against carbon tetrachloride induced hepatotoxicity.

Intractable Chronic Motor Tics Dramatically Respond to Clerodendrum inerme (L) Gaertn

Tics are characterized by involuntary, sudden, rapid, repetitive, nonrhythmic, stereotyped movements or phonic productions. Those who suffer from either motor or phonic tics, but not both, for more than 1 year are diagnosed with chronic tic disorder. Several pharmacological interventions have been proposed for the treatment of tic disorder. Dopamine D2 receptor blockers and dopamine depletors are thought to be the most effective ones clinically. However, such treatments are suboptimal in terms of effectiveness and side effects, such as body weight gain and extrapyramidal symptoms. We report on a 13-year-old girl, with chronic motor tic disorder refractory to multiple anti-tic therapies, who showed dramatic improvement and remission after taking the crude leaf extract of Clerodendrum inerme (L) Gaertn. No side effects were observed during a follow-up of more than 2 years. To the best of our knowledge, this is the first report on the anti-tic effect of Clerodendrum inerme.

FIELD EFFICACY OF PLANT EXTRACTS ON LARVAL POPULATIONS OF PLUTELLA XYLOSTELLA L. AND HELICOVERPA ARMIGERA HUB. AND THEIR IMPACT ON CABBAGE INFESTATION

ABSTRACT The field efficacy of four plant extracts such as Vitex negundo L. (Verbenaceae), Clerodendrum inerme (L.) Gaertn. (Verbenaceae), Lantana camara L. (Verbenaceae) and Eupatorium odoratum L. (Asteraceae) were evaluated against the diamondback moth, Plutella xylostella L. and the cotton bollworm, Helicoverpa armigera Hub. larvae on cultivated cabbage. Treatments with 1% V. negundo and C. inerme extracts significantly reduced P. xylostella larval density, and the percentage of infested plants, proving to be more effective than a standard insecticide Challenger 10EC (cypermethrin). Treatment with L. camara extract (1%) reduced the percentage of H. armigera infested plants and the intensity of cabbage damage. However, the plant extracts did not effectively reduce H. armigera larval density, and cabbage damage. Phytotoxic effects on cabbage plants were not observed in any extract treatment. Two plant extracts, V. negundo and C. inerme at 1% significantly reduced the P. xylostella larval density and proved more effective than Challenger. The intensity of cabbage damaged caused by P. xylostella was significantly lower in L. camara and C. inerme than control and Challenger.

Medicinal plants of Tamil Nadu (Southern India) are a rich source of antiviral activities

In order to evaluate the potential of medicinal plants of Tamil Nadu as sources of antiviral activities, we used seven different viruses to evaluate the methanol extracts of 30 plants, derived from 22 families and recognized for their local medical applications. Antiviral activity was the minimum concentration of extracts required to completely inhibit viral cytopathic effects (CPE), i.e., MIC100 values. Many extracts showed strong activities against Herpes simplex virus (HSV) and mouse corona virus (MCV, the surrogate for human SARS virus). Some extracts were also active against influenza virus and Sindbis virus (SINV, surrogate for hepatitis C virus), but fewer were active against the non-membrane viruses feline calicivirus (FCV, the surrogate for Norovirus), rhinovirus (common cold virus), and poliovirus. The most potent extracts (low MIC100 and broad spectrum of activity) were obtained from Gymnema sylvestre R. Br. (Asclepiadaceae), Pergularia daemia (Forsskal) Chiov. (Asclepiadaceae), Sphaeranthus indicus L. (Asteraceae), Cassia alata L. (Caesalpiniaceae), Evolvulus alsinoides L. (Convolvulaceae), Clitoria ternatea L. (Fabaceae), Indigofera tinctoria L. (Euphorbiaceae), Abutilon indicum G. Don. (Malvaceae), Vitex trifolia L. (Verbenaceae), Clerodendrum inerme (L.) Gaertn (Verbenaceae), and Leucas aspera Spr. (Lamiaceae), which showed anti-MCV and anti-HSV activities at a concentration as low as 0.4 μg/mL. In some cases the activities were enhanced by light, suggesting the presence of photosensitizers. Some of these antiviral activities could contribute to the medicinal properties of the plants, and also provide more support for the concept of scientific validation of traditional plant medicines in the fight against infectious diseases.

Hepatoprotective activity of Clerodendrum inerme against CCL 4 induced hepatic injury in rats

The ethanolic extract of Clerodendrum inerme leaves were screened for its hepatoprotective activity in CCl 4 (0.5 ml/kg, i.p) induced liver damage in Swiss albino rats at a dose of 200 mg /kg bw. The ethanolic extract of C. inerme significantly ( P < 0.001) decreases the serum enzyme alanine amino transferase (ALT), asparate amino transferase (AST), alkaline phosphates (ALP), triglycerides (TGL), total cholesterol (TC) and significantly increased the glutathione level. Silymarin (25 mg/kg), a known hepatoprotective drug used for comparison exhibited significant activity ( P < 0.001). The extract did not shown any mortality up to a dose of 2000 g/kg bw.

High‐performance thin‐layer chromatographic method for quantitative determination of 4α‐methyl‐24β‐ethyl‐5α‐cholesta‐14,25‐dien‐3β‐ol, 24β‐ethylcholesta‐5,9(11),22E‐trien‐3β‐ol, and betulinic acid in Clerodendrum inerme

A sensitive, selective, precise, and robust high-performance thin-layer chromatography method was developed and validated for analysis of two new recently isolated sterols, 4alpha-methyl-24beta-ethyl-5alpha-cholesta-14,25-dien-3beta-ol (1) and 24beta-ethylcholesta-5,9(11),22E-trien-3beta-ol (2), and a triterpene, betulinic acid (3), in Clerodendrum inerme extract. The method employed HPTLC plates precoated with silica gel 60F(254 )as the stationary phase. To achieve good separation, an optimised mobile phase consisting of toluene-acetone (94:06, v/v) was used (R(f )0.48, 0.34, and 0.22 for compounds 1, 2, and 3, respectively). Densitometric determination of the above compounds was carried out in reflection/absorption mode at 620 nm. Optimised chromatographic conditions provide well separated compact spots for the compounds 1, 2, and 3. The calibration curves were linear in the concentration range of 100-2500 ng/spot. The method was validated for precision, robustness, and recovery. The limits of detection and quantitation were 5, 6, and 10 microg/mL and 14, 18, and 29 microg/mL, respectively, for 1, 2, and 3. The method reported here is reproducible and convenient for quantitative analysis of these compounds in the aerial parts of C. inerme.