Eight wild plant species namely Tragia involucrata L., Cleistanthus collinus (Roxb.)Benth. Ex Hook.f., Sphaeranthus indicus L., Vicoa indica (L.) Dc., Allmania nodiflora (L.) R.Br. ex wight., Habenaria elliptica Wight., Eriocaulon thwaitesii Koern. and Evolvulus alsinoides L. were used for phytochemical extraction with four different solvents. Antibacterial activity of these plants was studied against Escherichia coli NCIM 2065 using Kirby Bauer agar disc diffusion assay. Effective antibacterial activity was shown by T. involucrata acetone extract (27.3mm), compared to standard medicinal drug amoxicillin (28.3mm). Minimum inhibitory concentration (MIC) of T. involucrata extract was 15mg/mL and hence, it could be pursued further for obtaining phytomedicine. Biochemical constituents of T. involucrata fresh leaf were: sugars (55mg/g), starch (0.7182mg/g), proteins (0.0166mg/g) and lipids (170mg/g). Alkaloids, tannins, phenolic compounds, flavonoids and steroids were also observed qualitatively.