Clay-rich Sediments Research Articles

Experimental slip distribution in lentils as an analog for scaly clay fabrics

Scaly fabrics are networks of anastomosing shear surfaces that are ubiquitous in deformed clay-rich sediments and fault gouges. Despite common occurrence, little is known about their behavior during deformation. It is not known whether they represent incremental slip on individual wavy shear surfaces, or whether multiple shear surfaces across a network deform simultaneously. We performed experiments on analog materials (oriented lentils) to observe the distribution of slip surfaces using a transparent shear box. We find that slip distributes through a network of anastomosing, transient slip surfaces that continually reorganize during shear. This is accompanied by local compaction and dilation so that volume changes caused by slip are balanced laterally between adjacent volumes. Lentils differ from equant granular materials because the shape inhibits grain rotation and facilitates interlocking. The self-perpetuating delocalization behavior of the system serves as a model for a conceptual understanding of how scaly fabrics deform and persist in natural clay-rich sediments and fault gouges.

Extant coccolithophores from the western equatorial Indian Ocean off Tanzania and coccolith distribution in surface sediments

Living coccolithophores and coccoliths from surface sediments were investigated in the western equatorial Indian Ocean off Tanzania, from the coast and Pemba Channel to the open ocean. In total, 49 plankton samples from 8 stations and 20 surface sediment samples collected in February 2008 were analysed. Coccolithophore standing stock ranged from barren up to almost 23,000 coccospheres/l in the upper water column and the living community comprised 47 different taxa in total. In addition, six more species were only found as detached coccoliths. Coccolithophore numbers were generally low and only Florisphaera profunda, Gephyrocapsa oceanica, and Emiliania huxleyi were numerically important (>5,000 coccospheres/l). Two distinct depth-related assemblages were identified. The upper photic zone (down to about 50m) in the Pemba Channel was dominated by G. oceanica and can be related to slightly increased nutrient conditions. In contrast, the upper photic zone community in the open ocean (down to about 70m) is characterised by high numbers of Umbellosphaera irregularis, typically indicating oligotrophic stratified waters, high sea-surface temperatures and strong water stratification. The lower photic-zone flora in the whole study area is generally dominated by F. profunda and accompanying species. A total of 40 species were identified in the surface sediments and total coccolith numbers vary drastically from <1000 to 18,200 x 106 coccoliths/g of sediment. The absolute numbers are much lower near-shore and strikingly enhanced further offshore mainly due to heavy dilution by fine-grained terrigenous sediments. Nevertheless, the occurrence of species in the plankton communities compares well to those of the surface sediment assemblages although plankton samples are from just one season. This points to an excellent preservation potential in this area, which can be explained by high supply of clay-rich sediments and a therefore rapid burial of the coccoliths in shelf and upper slope sediments.

Modern and past periglacial features in Central Asia and their implication for paleoclimate reconstructions

In the continental areas of Central and High Asia, periglacial landform assemblages, sediment structures and processes are mainly influenced and determined by of soil humidity during freeze–thaw cycles. These cryogenic processes result in periglacial landforms such as solifluction, earth hummocks or patterned ground. The distribution of rock glaciers as clear indicators of permafrost is additionally determined by rock fall or moraine debris composed of large boulders (e.g. of granite). Periglacial features were used to reconstruct past climatic conditions, e.g. relict involutions and ice-wedge casts provide evidence for the distribution of former permafrost, say, for the Last Glacial Maximum (LGM). Past temperatures, e.g. mean annual air temperatures, can be estimated from these periglacial features and can be compared with other proxy data, such as glacier fluctuations. Examples from late Holocene solifluction activity in the Altai, Khangai and north-eastern Tibetan Plateau show a different intensity of solifluction processes during the late Holocene and Little Ice Age due to a decrease in temperature and higher soil humidity. The distribution of past permafrost in some regions is still a matter of debate because of different interpretations of sediment structures: sometimes features described as ice-wedge casts may be caused by roots or desiccation cracks due to drying of clay rich sediments. Seismically deformed unconsolidated deposits (seismites) can also be misinterpreted as periglacial involutions. The lack of certain landform assemblages and sediment structures does not necessarily mean that the area had no permafrost. Moisture conditions can also determine the periglacial landform generation to a large degree. They can be ordered in Central Asia as follows (from highest moisture availability to lowest): solifluction; rock glacier; permafrost involutions; ice-wedge casts; sand-wedge casts.

Propidium monoazide treatment to distinguish between live and dead methanogens in pure cultures and environmental samples

In clinical trials investigating human health and in the analysis of microbial communities in cultures and natural environments, it is a substantial challenge to differentiate between living, potentially active communities and dead cells. The DNA-intercalating dye propidium monoazide (PMA) enables the selective masking of DNA from dead, membrane-compromised cells immediately before DNA extraction. In the present study, we evaluated for the first time a PMA treatment for methanogenic archaea in cultures and particle-rich environmental samples. Using microscopic analyses, we confirmed the applicability of the LIVE/DEAD® BacLight™ kit to methanogenic archaea and demonstrated the maintenance of intact cell membranes of methanogens in the presence of PMA. Although strain-specific differences in the efficiency of PMA treatment to methanogenic archaea were observed, we developed an optimal procedure using 130μM PMA and 5min of photo-activation with blue LED light. The results showed that the effectiveness of the PMA treatment strongly depends on the texture of the sediment/soil: silt and clay-rich sediments represent a challenge at all concentrations, whereas successful suppression of DNA from dead cells with compromised membranes was possible for low particle loads of sandy soil (total suspended solids (TSS)≤200mgmL−1). Conclusively, we present two strategies to overcome the problem of insufficient light activation of PMA caused by the turbidity effect (shielding) in particle-rich environmental samples by (i) dilution of the particle-rich sample and (ii) detachment of the cells and the free DNA from the sediment prior to a PMA treatment. Both strategies promise to be usable options for distinguishing living cells and free DNA in complex environmental samples.

The depositional conditions and the palaeoenvironment of the Achlada xylite-dominated lignite in western Makedonia, Greece

The depositional palaeoenvironment of the Neogene Achlada lignite deposit (western Makedonia, Greece) was deciphered with respect to the evaluation of the macro-petrographic characteristics, and the data resulted from coal petrographic analyses.The total thickness of the studied lignite seam is ~26m and consists of organic and inorganic alternations. The macro-petrographic observations suggest extremely high contributions of woody-originated tissue (xylite) from a single tree type, among the lignite layers, whereas, numerous in situ stems and trunks were observed. The results of the coal petrographic analysis indicate the dominance of huminite (88.3vol.%), whereas liptinite and inertinite show much lower values (11.0vol.% and 0.7vol.% respectively). High contribution of mineral matter is also observed in many cases. Ulminite types A and B are the most abundant macerals, whereas the content of ulminite B is followed by the xylite content. Facies modeling using maceral composition and maceral indices suggested the alternation of forest swamp and reed-moor environments where peat accumulated under highly-saturated and intensely-anoxic conditions. A terrestrial origin with wet forest to piedmont plain depositional conditions was suggested by the Gelification Index (GI) and Tissue Preservation Index (TPI) plot. A cross-plot of the ground water index (GWI) vs. the vegetation index (VI) suggested swamps under mesotrophic and rheotrophic hydrogeological conditions, where the dominant source for the peat was woody vegetation and vegetation high in preservation potential.The overall macro and micro characteristics of the Achlada lignite suggest a depositional palaeoenvironment that includes three independent parts: a) the main channel of a meandering river system; b) the floodplain area; and c) the oxbow lake. The establishment of telmatic/limnotelmatic conditions in this palaeoenvironment resulted in the formation of the entire Achlada lignite deposit, whereas numerous flood episodes interrupted the peat accumulation and resulted in the deposition of clay-rich sediments.

Quantified abundance of magnetofossils at the Paleocene–Eocene boundary from synchrotron-based transmission X-ray microscopy

The Paleocene-Eocene boundary (∼55.8 million years ago) is marked by an abrupt negative carbon isotope excursion (CIE) that coincides with an oxygen isotope decrease interpreted as the Paleocene-Eocene thermal maximum. Biogenic magnetite (Fe3O4) in the form of giant (micron-sized) spearhead-like and spindle-like magnetofossils, as well as nano-sized magnetotactic bacteria magnetosome chains, have been reported in clay-rich sediments in the New Jersey Atlantic Coastal Plain and were thought to account for the distinctive single-domain magnetic properties of these sediments. Uncalibrated strong field magnet extraction techniques have been typically used to provide material for scanning and transmission electron microscopic imaging of these magnetic particles, whose concentration in the natural sediment is thus difficult to quantify. In this study, we use a recently developed ultrahigh-resolution, synchrotron-based, full-field transmission X-ray microscope to study the iron-rich minerals within the clay sediment in their bulk state. We are able to estimate the total magnetization concentration of the giant biogenic magnetofossils to be only ∼10% of whole sediment. Along with previous rock magnetic studies on the CIE clay, we suggest that most of the magnetite in the clay occurs as isolated, near-equidimensional nanoparticles, a suggestion that points to a nonbiogenic origin, such as comet impact plume condensates in what may be very rapidly deposited CIE clays.

Geological and taphonomic context for the new hominin species Homo naledi from the Dinaledi Chamber, South Africa

We describe the physical context of the Dinaledi Chamber within the Rising Star cave, South Africa, which contains the fossils of Homo naledi. Approximately 1550 specimens of hominin remains have been recovered from at least 15 individuals, representing a small portion of the total fossil content. Macro-vertebrate fossils are exclusively H. naledi, and occur within clay-rich sediments derived from in situ weathering, and exogenous clay and silt, which entered the chamber through fractures that prevented passage of coarser-grained material. The chamber was always in the dark zone, and not accessible to non-hominins. Bone taphonomy indicates that hominin individuals reached the chamber complete, with disarticulation occurring during/after deposition. Hominins accumulated over time as older laminated mudstone units and sediment along the cave floor were eroded. Preliminary evidence is consistent with deliberate body disposal in a single location, by a hominin species other than Homo sapiens, at an as-yet unknown date.

A 94–10ka pollen record of vegetation change in Qaidam Basin, northeastern Tibetan Plateau

Drill core (ISL1A) was obtained from the Qarhan Salt Lake in central eastern Qaidam Basin, northeastern Tibetan Plateau (NE TP). Fossil pollen and the lithology of the core sediment were analyzed in conjunction with AMS C-14 and Th-230 dating. The results indicated that Artemisia and Chenopodiaceae dominated the steppe/desert steppe vegetation developed around the lake between 94 and 51.2 ka, corresponding with the organic-rich silty clay deposited in the core sediments. Pediastrum continuously appeared in the core sediments between 94 and 51.2 ka, indicating freshwater to oligohaline conditions of the paleo-Qarhan Lake during the late marine isotope stage (MIS) 5, MIS 4, and early MIS 3. During the 51.2 to 32.5 ka period, Ephedra dominated shrub-desert vegetation expanded in the basin, while, Pediastrum disappeared in the core sediments. The core sediments consisted of interbedded layers of halite silt and clay-rich halite between 51.2 and 32.5 ka, signifying a shift toward drier hydrologic conditions. Thus, the paleo-Qarhan Lake experienced periods of desiccation and shallow water levels. Artemisia and Chenopodiaceae dominated steppe/desert steppe vegetation appeared again from 32.5 to 253 ka, with clay-rich sediments in the core, thereby suggesting an increase of runoff from the lake under relatively wetter climatic conditions. Since 253 ka, however, pollen concentrations declined, corresponding with the amount of halite deposited in the core sediment and, suggesting a cold, dry climatic condition during MIS2. Our findings have important implications for understanding complex regional vegetation and climatic responses to large-scale forcings in the Qaidam Basin. (C) 2015 Elsevier B.V. All rights reserved.

Strength characteristics of Japan Trench borehole samples in the high-slip region of the 2011 Tohoku-Oki earthquake

The 2011 Tohoku-Oki earthquake demonstrated that the shallowest reaches of plate boundary subduction megathrusts can host substantial coseismic slip that generates large and destructive tsunamis, contrary to the common assumption that the frictional properties of unconsolidated clay-rich sediments at depths less than ∼5km should inhibit rupture. We report on laboratory shearing experiments at low sliding velocities (<1mm/s) using borehole samples recovered during IODP Expedition 343 (JFAST), spanning the plate-boundary décollement within the region of large coseismic slip during the Tohoku earthquake. We show that at sub-seismic slip rates the fault is weak (sliding friction μs=0.2–0.26), in contrast to the much stronger wall rocks (μs>∼0.5). The fault is weak due to elevated smectite clay content and is frictionally similar to a pelagic clay layer of similar composition. The higher cohesion of intact wall rock samples coupled with their higher amorphous silica content suggests that the wall rock is stronger due to diagenetic cementation and low clay content. Our measurements also show that the strongly developed in-situ fabric in the fault zone does not contribute to its frictional weakness, but does lead to a near-cohesionless fault zone, which may facilitate rupture propagation by reducing shear strength and surface energy at the tip of the rupture front. We suggest that the shallow rupture and large coseismic slip during the 2011 Tohoku earthquake was facilitated by a weak and cohesionless fault combined with strong wall rocks that drive localized deformation within a narrow zone.

Mineralogical, chemical composition and distribution of rare earth elements in clay-rich sediments from Southeastern Nigeria

Cretaceous claystone sediments from Enugu, Southeastern, Nigeria were analyzed for their mineralogy and chemistry. Major minerals are quartz and kaolinite while montmorillonite is in minor quantity. The sediments are silica-rich, but showed low values of Al, Fe, Sc and Cr. The values of the chemical index of alteration (CIA) ranged from 89.9 to 94.5 and the values of chemical index of weathering (CIW) ranged from 95.1 to 98.9. Low contents of the alkali and alkali earth elements (Na, K, Mg, Al, Ca) of the clay-rich sediments suggest a relatively more intense weathering of source area. Depleted Ba, Rb, Ca, and Mg suggest that they were probably flushed out by water during sedimentation.The mineralogical composition, REE contents, and elemental ratios in the sediments suggest a provenance from mainly felsic rocks, with only minor contributions from basic sources. Despite intense weathering the REE, Th, and Sc remained in the clays suggesting that they were immobile.

Clastic dikes in the Dead Sea basin as indicators of local site amplification

Early Holocene seismic activity triggered fluidization and clastic-dike emplacement within Late Pleistocene lacustrine Lisan Formation sediments in the Dead Sea basin (DSB). Hundreds of opening-mode clastic dikes were documented cross-cutting the Lisan Formation in four distinct sites in relatively small ( 6.5). The injection of clastic dikes in distinct and relatively small areas in the Lisan Formation along the DSB may serve as evidence for a unique combination of three factors: (a) clay-rich sediments and high water table as favorable conditions for fluidization on the exposed surface following the Lake Lisan regression; (b) the occurrence of moderate-to-strong earthquakes (M w > 6) in the early Holocene after the deposition of the Lisan Formation; and (c) the local geological structure characterized by edge and basin effects, which can amplify seismic waves. The present study indicates that injection due to fluidization of clay-rich strata during earthquakes was dominant throughout the DSB. We suggest that the clastic dikes can serve as evidence for local amplification conditions following seismic processes that occur close to active faults.

The Recognition of Hailstone Impressions In Clay-Rich Sediment: Experimental Results and Relation To the Neoproterozoic Case

Abstract Based on experimental impacts of hailstone and rain droplets on clayey sediment, distinct differences between their imprints are shown. The key difference that unequivocally differentiates these impact structures is that raindrop imprints are always part of a sphere, even after different degrees of compaction, whereas hailstone imprints never have such a shape, forming a more or less regular funnel, terminated by a “nipple-like” structure that usually retains the original shape of the hailstone. We show and discuss the reasons why hailstone imprints have never been reported in the sedimentary record, albeit their fossilization potential seems to be much higher than that of raindrop imprints, which are commonly reported from various types of sedimentary rocks of widely differing ages. Inferred hailstone imprints are illustrated from the fossil record for the first time, from the Neoproterozoic–Cambrian transition of western Africa. The origin of paleohailstones required specific dynamic conditions of the atmosphere similar to those of the Recent atmosphere. Present-day hailstorms are generally restricted to specific geographic regions, e.g., mid-latitude, along mountain ranges, and they are largely absent from circum-polar and circum-equatorial areas. Correspondingly, hailstone impact structures preserved in the sedimentary record may possess interpretational value, regarding fundamental questions concerning the dynamics of the paleoatmosphere and perhaps paleogeography of ancient continents.

Method for estimating ductile horizontal strain from magnetic fabrics in poorly consolidated clay-rich sediments

We propose a simple method based on the normalized eigenvalues of the tensor of anisotropy of magnetic susceptibility (AMS) to estimate horizontal ductile strain in detrital clay-rich sedimentary rocks subjected to layer parallel shortening (LPS). This method is mainly based on two assumptions: (1) The magnetic carriers are contacting clay-mineral platelets, and (2) The platelets respond to LPS by crenulation and formation of a zone axis of unchanged magnetic susceptibility perpendicular to the plane of deformation. We further simplify the problem by considering, as the exact distribution of particles orientations is unknown, that the AMS tensor arises from a single equivalent clay platelet, of which the angle with the bedding plane directly reflects the amount of horizontal strain. The proposed method is applied to data from samples retrieved during Integrated Ocean Drilling Program (IODP) Expeditions 315 and 316 of the Nankai Trough Seismogenic Zone Experiment (NanTroSEIZE) complex drilling project. Sample-wise finite strain values assist AMS data interpretation, which is traditionally based on the AMS ellipsoid shape parameter, and allow comparison with strain estimates obtained by other means. In the Nankai Accretionary Prism, AMS-derived strain values range from 0 to 25% and suggest substantial mechanical compartmentalization with inter-unit strain contrasts larger than 5 percentage points, including the penetrated shallow portion of a megasplay fault. At a local scale, AMS strain also appears to be affected by thrusting, what can be interpreted as a record of a ductile strain component away for the brittle zone or as an ‘interseismic’ ductile strain within otherwise brecciated material.

Frictional properties of incoming pelagic sediments at the Japan Trench: implications for large slip at a shallow plate boundary during the 2011 Tohoku earthquake

The 2011 Tohoku earthquake (Mw 9.0) produced a very large slip on the shallow part of a megathrust fault that resulted in destructive tsunamis. Although multiple causes of such large slip at shallow depths are to be expected, the frictional property of sediments around the fault, particularly at coseismic slip velocities, may significantly contribute to large slip along such faults. We have thus investigated the frictional properties of incoming pelagic sediments that will subduct along the plate boundary fault at the Tohoku subduction zone, in order to understand the rupture processes that can cause large slip in the shallow parts of subduction zones. Our experimental results on clayey sediment at the base of the sedimentary section on the Pacific Plate yield a low friction coefficient of <0.2 over a wide range of slip velocities (0.25 mm/s to 1.3 m/s), and extremely low fracture energy during slip weakening, as compared with previous experiments of disaggregated sediments under coseismic slip conditions. Integrated Ocean Drilling Program (IODP) Expedition 343 confirmed that the clay-rich sediment investigated here is identical to those in the plate boundary fault zone, which ruptured and generated the Tohoku earthquake. The present results suggest that smectite-rich pelagic sediment not only accommodates cumulative plate motion over interseismic periods but also energetically facilitates the propagation of earthquake rupture towards the shallow part of the Tohoku subduction zone.

Erratum to: An integrated magnetic susceptibility anisotropy (AMS) and structural geological study on Cenozoic clay-rich sediments from the Transdanubian Range

"Erratum to: An integrated magnetic susceptibility anisotropy (AMS) and structural geological study on Cenozoic clay-rich sediments from the Transdanubian Range" published on 27 Dec 2014 by Akadémiai Kiadó.

Reduction and immobilization of hexavalent chromium by microbially reduced Fe-bearing clay minerals

Hexavalent chromium (Cr6+) is a major contaminant in the environment. As a redox-sensitive element, the fate and toxicity of chromium is controlled by reduction-oxidation (redox) reactions. Previous research has shown the ability of structural Fe(II) in naturally present and chemically reduced clay minerals to reduce Cr6+ to Cr(III) as a way of immobilization and detoxification. However, it is still poorly known whether or not structural Fe(II) in biologically reduced clay minerals exhibits a similar reactivity and if so, what the kinetics and mechanisms of Cr6+ reduction are. The objective of this study was to determine the kinetics and possible mechanisms of Cr6+ reduction by structural Fe(II) in microbially reduced clay minerals and the nature of reduced Cr(III). Structural Fe(III) in nontronite (NAu-2), montmorillonite (SWy-2), chlorite (CCa-2), and clay-rich sediments from the Ringold Formation of the Hanford site of Washington State, USA was first bioreduced to Fe(II) by an iron-reducing bacterium Geobacter sulfurreducens with acetate as the sole electron donor and anthraquinone-2,6-disulfonate (AQDS) as electron shuttle in synthetic groundwater (pH 7). Biogenic Fe(II) was then used to reduce aqueous Cr6+ at three different temperatures, 10, 20, and 30°C, in order to determine the temperature dependence of the redox reaction between Cr6+ and clay-Fe(II). The results showed that nontronite and montmorillonite were most effective in reducing aqueous Cr6+ at all three temperatures. In contrast, most Fe(II) in chlorite was not reactive towards Cr6+ reduction at 10°C, though at 30°C there was some reduction. For all the clay minerals, the ratio of total Fe(II) oxidized to Cr6+ reduced was close to the expected stoichiometric value of 3. Characterization of the Cr-clay reaction product with scanning electron microscopy with focused ion beam and transmission electron microscopy with electron energy loss spectroscopy revealed that reduced chromium was possibly in the form of sub-nanometer Cr2O3 in association with residual clay minerals as micro-aggregates. This textural association was expected to minimize the chance of Cr(III) reoxidation upon exposure to oxidants. These results are important for our understanding of how various clay minerals may be used to reductively immobilize the heavy metal contaminant Cr in the environment.

An integrated magnetic susceptibility anisotropy (AMS) and structural geological study on Cenozoic clay-rich sediments from the Transdanubian Range

Systematic structural and anisotropy of magnetic susceptibility (AMS) measurements were carried out on Cenozoic clay-rich deposits from the Transdanubian Range, central part of the Alcapa Unit. The aim was to improve the knowledge of the Neogene tectonic evolution of the area and of the connection of the stress field and the magnetic fabric of the sediments. The measurements of AMS revealed dominant foliation with weak lineation for Middle Eocene-Lower Miocene sediments. The directions of AMS lineation are aligned either with the direction of NNE-SSW extension of a strike slip phase (30–19 Ma) or with the direction of NE-SW extension of the main rifting phase of the Pannonian Basin (19–14 Ma).The studied Late Miocene sediments have foliated AMS fabric, maximum and intermediate AMS directions are intermixed, and the AMS fabrics do not show any sign of tectonic deformation. In contrast, joints and faults were observed in the same rocks. Detailed structural analysis shows two extensional phases between ca. 1...

Geochemical characteristics and genetic types of crude oils from Qinjiatun and Qikeshu oilfields in the Lishu Fault Depression, Songliao Basin, northeastern China

The Qinjiatun and Qikeshu oilfields are new Mesozoic petroleum exploration targets in Lishu Fault Depression of Songliao Basin, northeastern China. Currently, researches on geochemistry of crude oils from Qinjiatun and Qikeshu oilfields have not been performed and the genesis of oils is still uncertain. Based on bulk analyses, the crude oils in the Qinjiatun and Qikeshu oilfields of Lishu Fault Depression from the Lower Cretaceous can be classified as three types. Type oils I, from Quantou and Denglouku formations of Qikeshu oilfield, are characterized by high C24tetracyclic terpane/C26tricyclic terpanes ratios, low gammacerance/C30hopane ratios, tricyclic terpanes/ hopanes ratios, C29Ts/C29norhopane ratios and 17α(H)-diahopane/17α(H)-hopane ratios, indicating a brackish lacustrine facies. Type oils II, from Shahezi Formation of Qikeshu oilfield show low C24tetracyclic terpane/C26tricyclic terpanes, high gammacerance/C30hopane ratios, tricyclic terpanes/hopanes ratios, C29Ts/C29 norhopane and C30diahopane/C30hopane ratios, thus suggesting that they originated from source rocks deposited in a weak reducing brackish lacustrine environment, or clay-rich sediments. Type oils III, from some wells of Qikeshu oilfield have geochemical characteristics intermediate between those two types and may be mixture of type I and II oils.

Lack of &amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C-label incorporation suggests low turnover rates of thaumarchaeal intact polar tetraether lipids in sediments from the Iceland shelf

Abstract. Thaumarchaeota are amongst the most abundant microorganisms in aquatic environments, however, their metabolism in marine sediments is still debated. Labeling studies in marine sediments have previously been undertaken, but focused on complex organic carbon substrates which Thaumarchaeota have not yet been shown to take up. In this study, we investigated the activity of Thaumarchaeota in sediments by supplying different 13C-labeled substrates which have previously been shown to be incorporated into archaeal cells in water incubations and/or enrichment cultures. We determined the incorporation of 13C-label from bicarbonate, pyruvate, glucose and amino acids into thaumarchaeal intact polar lipid-glycerol dibiphytanyl glycerol tetraethers (IPL-GDGTs) during 4–6 day incubations of marine sediment cores from three sites on the Iceland shelf. Thaumarchaeal intact polar lipids, in particular crenarchaeol, were detected at all stations and concentrations remained constant or decreased slightly upon incubation. No 13C incorporation in any IPL-GDGT was observed at stations 2 (clay-rich sediment) and 3 (organic-rich sediment). In bacterial/eukaryotic IPL-derived fatty acids at station 3, contrastingly, a large uptake of 13C label (up to + 80‰ ) was found. 13C was also respired during the experiment as shown by a substantial increase in the 13C content of the dissolved inorganic carbon. In IPL-GDGTs recovered from the sandy sediments at station 1, however, some enrichment in δ13C (1–4‰ ) was detected after incubation with bicarbonate and pyruvate. The low incorporation rates suggest a low activity of Thaumarchaeota in marine sediments and/or a low turnover rate of thaumarchaeal IPL-GDGTs due to their low degradation rates. Cell numbers and activity of sedimentary Thaumarchaeota based on IPL-GDGT measurements may thus have previously been overestimated.

Investigating the behaviour of Mg isotopes during the formation of clay minerals

We present elemental and isotopic data detailing how the Mg isotope system behaves in natural and experimentally synthesized clay minerals. We show that the bulk Mg isotopic composition (δ26Mg) of a set of natural illite, montmorillonite and kaolinite spans a 2‰ range, and that their isotopic composition depends strongly on a balance between the relative proportions of structural and exchangeable Mg. After acid leaching, these natural clays become relatively enriched in isotopically heavy Mg by between 0.2‰ and 1.6‰. Results of exchange experiments indicate that the Mg that has adsorbed to interlayer spaces and surface charged sites is relatively enriched in isotopically light Mg compared to the residual clay. The isotopic composition of this exchangeable Mg (−1.49‰ to −2.03‰) is characteristic of the isotopic composition of Mg found in many natural waters. Further experiments with an isotopically characterized MgCl2 solution shows that the clay minerals adsorb this exchangeable Mg with little or no isotopic fractionation, although we cannot discount the possibility that the uptake of exchangeable Mg does so with a slight preference for 24Mg. To characterize the behaviour of Mg isotopes during clay mineral formation we synthesized brucite (Mg(OH)2), which we consider to be a good analogue for the incorporation of Mg into the octahedral sheet of Mg-rich clay minerals or into the brucitic layer of clays such as chlorite. In our experiment the brucite mineral becomes enriched in the heavy isotopes of Mg while the corresponding solution is always relatively enriched in isotopically light Mg. The system reaches a steady state after 10days with a final fractionation factor (αsolid-solution) of 1.0005 at near-neutral pH. This result is consistent with the general consensus that secondary clay minerals preferentially take up isotopically heavy Mg during their formation. However our results also show that exchangeable Mg is an important component within bulk clay minerals and can have an important influence over the bulk clay δ26Mg value. Modeling shows that in certain soils or sediments where the percentage of exchangeable Mg is >30% and the isotopic composition of the exchangeable Mg is around −2‰, the generation of bulk δ26Mg values of <−0.5‰ is likely. On a broader scale, Mg-rich minerals such as smectite and illite are likely to impart a stronger control over the Mg budget in clay rich sediments, and their high structural Mg component is likely to result in bulk sediment δ26Mg values that are closer in composition to the UCC. Despite this, results of modeling, together with experimental observation suggests that the uptake of exchangeable Mg into these clay rich sediments could cause a decrease in the bulk δ26Mg value by up to ∼0.3–0.4‰. This should be accounted for when assessing the δ26Mg value of sediments on a crustal scale.