Classical Culture Research Articles

The School of Bunesti, Arges, Romania: Hand-made Architecture, A Craft of Self-limitation

Weary of the present-day inflation of possibilities for planning, building, learning, and living, and with an increasingly acute sense of the artificiality of our professional training, we—a historian, an architect, and a small group of students—chose to get away from urban scenery and to put ourselves to the test through a summer. We settled in the middle of a forest, giving ourselves time for reflection and action from a radically different perspective. Inspired by classic authors and classical culture, since 2008 we have been taking the local natural building techniques of a Wallachian valley as a guide to the timeless topography of the ancient world.

A Contrastive Study of Chinese-English Translation of Ancient Poetry from the Perspective of Aesthetics of Reception: A Case Study of the Two Translations of Vain Longing

The Chinese literary classic "Dream of the Red Chamber" features a variety of poems and songs, with "Vain Longing," which serves as its theme song, being particularly popular among readers both domestically and internationally. Among the many translations available, those by Yang Xianyi and Gladys Yang, along with Hawkes' version, are the most prominent and hold significant research value. Acceptance Aesthetics, which emerged from the German critical school in the 20th century, provides a fresh lens through which to view the translation of ancient Chinese poetry. This paper utilizes Aesthetics of reception as its theoretical framework and focuses on the English translations of "Vain Longing" by Yang Xianyi and Gladys Yang as well as Hawkes found in "Dream of the Red Chamber." By examining their translation philosophies and approaches, this study aims to contribute insights for translating ancient poetry within literary contexts while enhancing global appreciation for Chinese poetic beauty and promoting classical Chinese culture.

The Structure of the Late Bronze Age Population of Western Siberia: Craniometric Evidence

To assess the sources of population differentiation in Late Bronze Age Western Siberia, measurements of 68 cranial samples of this and earlier periods were processed with multivariate statistical methods. Results support the idea of at least two post-Afanasyevo migrations to Siberia from the west—pre-Andronovo and Andronovo. The former was represented by Chaa-Khol, Yelunino, and Samus people. Those associated with Karakol culture partly resemble the above and partly both autochthonous populations—that of Baraba (“Northern Eurasian formation”) and that of Okunev culture (“Southern Eurasian formation”), which appear to be two extremes of a single continuum. Differences between the two Andronovo traditions, Fedorovka and Alakul, are likely due to the local substratum in the former rather than to various origins. The Karasuk group arose through admixture between Okunev and Andronovo. People associated with the classic Karasuk culture are closer to the former, while those of the Kamenny Log stage tend toward the latter. People of the Upper Irtysh and the Mongun-Taiga people from Baidag III resemble those of Karasuk. Two pooled groups, Irmen and Mongun-Taiga, and the Pakhomovskaya sample indicate a possible admixture between both autochthonous formations, Northern and Southern, as well as Andronovo and Karasuk. Among the so-called Andronoid groups, only Yelovka and Pakhomovskaya, as well as a sample from Yelovka I, suggest admixture between Andronovans and Western Siberian natives, while Cherkaskul and Korchazhka, like the Late Krotovo groups from Sopka and Cherno-Ozerye and the Begazy-Dandybai group of Baraba, deviate from the Northern Eurasian formation toward Okunev rather than Andronovo. Among the two Eurasian formations, the Southern one (i.e., Okunev) was more affected by admixture between the autochthones and the immigrants.

Utility of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry in Periprosthetic Joint Infection Diagnosis.

One of the most feared complications of arthroplasty surgery is septic loosening. Periprosthetic joint infection (PJI) requires an accurate and fast diagnosis, and identification of pathogen microorganisms is essential for successful treatment. While standard bacteriological cultures can identify bacteria in seven to 14 days with sensitivity ranging from 35% to 70% that could further be increased by sonication of the explanted prosthesis, we would like to review a more novel and faster method of PJI detection and bacterial identification. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/MS) is a technique that identifies bacteria based on peptides and protein ions from the cell surface, comparing the obtained results within a database. While MALDI-TOF/MS is not a novel method, being already successfully used in microbiology, its role in PJI is still being researched. With this paper, we would like to reveal the current state of development in implementing MALDI-TOF/MS as an alternative or auxiliary test to classic bacterial cultures in orthopedic implant infectious pathology to increase the accuracy of detecting and identifying bacteria.

Microfluidic Applications in Prostate Cancer Research.

Prostate cancer is a disease in which cells in the prostate, a gland in the male reproductive system below the bladder, grow out of control and, among men, it is the second-most frequently diagnosed cancer (other than skin cancer). In recent years, prostate cancer death rate has stabilized and, currently, it is the second-most frequent cause of cancer death in men (after lung cancer). Most deaths occur due to metastasis, as cancer cells from the original tumor establish secondary tumors in distant organs. For a long time, classical cell cultures and animal models have been utilized in basic and applied scientific research, including clinical applications for many diseases, such as prostate cancer, since no better alternatives were available. Although helpful in dissecting cellular mechanisms, these models are poor predictors of physiological behavior mainly because of the lack of appropriate microenvironments. Microfluidics has emerged in the last two decades as a technology that could lead to a paradigm shift in life sciences and, in particular, controlling cancer. Microfluidic systems, such as organ-on-chips, have been assembled to mimic the critical functions of human organs. These microphysiological systems enable the long-term maintenance of cellular co-cultures in vitro to reconstitute in vivo tissue-level microenvironments, bridging the gap between traditional cell cultures and animal models. Several reviews on microfluidics for prostate cancer studies have been published focusing on technology advancement and disease progression. As metastatic castration-resistant prostate cancer remains a clinically challenging late-stage cancer, with no curative treatments, we expanded this review to cover recent microfluidic applications related to prostate cancer research. The review includes discussions of the roles of microfluidics in modeling the human prostate, prostate cancer initiation and development, as well as prostate cancer detection and therapy, highlighting potentially major contributions of microfluidics in the continuous march toward eradicating prostate cancer.

Estimating pneumococcal carriage dynamics in adults living with HIV in a mature infant pneumococcal conjugate vaccine programme in Malawi, a modelling study

BackgroundAdults living with human immunodeficiency virus (ALWHIV) receiving antiretroviral therapy (ART) exhibit higher pneumococcal carriage prevalence than adults without HIV (HIV-). To assess factors influencing high pneumococcal carriage in ALWHIV, we estimated pneumococcal carriage acquisition and clearance rates in a high transmission and disease-burdened setting at least 10 years after introducing infant PCV13 in routine immunisation.MethodsWe collected longitudinal nasopharyngeal swabs from individuals aged 18–45 in Blantyre, Malawi. The study group included both HIV- individuals and those living with HIV, categorised based on ART duration as either exceeding 1 year (ART > 1y) or less than 3 months (ART < 3 m). Samples were collected at baseline and then weekly for 16 visits. To detect pneumococcal carriage, we used classical culture microbiology, and to determine pneumococcal serotypes, we used latex agglutination. We modelled trajectories of serotype colonisation using multi-state Markov models to capture pneumococcal carriage dynamics, adjusting for age, sex, number of under 5 year old (< 5y) children, social economic status (SES), and seasonality.ResultsWe enrolled 195 adults, 65 adults in each of the study groups. 51.8% were females, 25.6% lived with more than one child under 5 years old, and 41.6% lived in low socioeconomic areas. The median age was 33 years (IQR 25–37 years). The baseline pneumococcal carriage prevalence of all serotypes was 31.3%, with non-PCV13 serotypes (NVT) at 26.2% and PCV13 serotypes (VT) at 5.1%. In a multivariate longitudinal analysis, pneumococcal carriage acquisition was higher in females than males (hazard ratio [HR], NVT [1.53]; VT [1.96]). It was also higher in low than high SES (NVT [1.38]; VT [2.06]), in adults living with 2 + than 1 child < 5y (VT [1.78]), and in ALWHIV on ART > 1y than HIV- adults (NVT [1.43]). Moreover, ALWHIV on ART > 1y cleared pneumococci slower than HIV- adults ([0.65]). Residual VT 19F and 3 were highly acquired, although NVT remained dominant.ConclusionsThe disproportionately high point prevalence of pneumococcal carriage in ALWHIV on ART > 1y is likely due to impaired nasopharyngeal clearance, which results in prolonged carriage. Our findings provide baseline estimates for comparing pneumococcal carriage dynamics after implementing new PCV strategies in ALWHIV.

Jesuits, Jansenists, and the Culture Wars

Abstract This introductory article explores the relevance of studying the Jesuit-Jansenist conflict in early modern France against the broader background of modernity as such and its inherent problems with fragmentation, isolation, and polarization in all areas of culture. It begins with a reference to the particular use of the study of early modernity and its religious and sociopolitical problems by the two prominent dissidents of the twentieth century, Leszek Kołakowski (1927–2009) and Jan Patočka (1907–77), in order to draw some potential parallels between the contemporary “culture wars” and the conflict between the Jesuits and the Jansenists. The contributions, stemming from a conference held at Adam Mickiewicz University in Poznań in March 2023, celebrating the four-hundredth anniversary of the birth of Blaise Pascal, shed light on various aspects of classical culture, education, tradition, and innovation in the areas of theatre, music, and philosophy in seventeenth-century France.

Life criticism and De Quincey's ethical concern over the aestheticiability of murder

AbstractThe self‐reflexive tendency in De Quincey's seemingly Kantian aestheticization of murder allows some leeway in deciphering his underlying ethical concerns. As “On the knocking at the gate in Macbeth” shows, De Quincey engages in a special kind of life writing that can be termed “life criticism,” which is not naively referential but revelatory, translating the autobiographical records of memories, experiences, and emotions into mediatory possibilities for argumentative assessments of literary and aesthetic questions. James Wood's critiques function as a compelling touchstone of De Quincey's life criticism, exhibiting how self‐referential episodes, as both the starting and end points for literary criticism, can be incorporated into serious and illuminating theoretical interrogations. Through the new prism of De Quincey's habitual mode of life criticism, his aesthetics of murder can be interpreted as a meta‐reflection upon his literary experiences with Westmorland Gazette and Blackwood's Magazine. His awareness of the facile aestheticiability of murder in the periodical press has an obvious moral implication: it shows his self‐reflexive concern over the commercialized public taste that makes him torn between insisting upon classical highbrow culture and contributing sensational magazine reading matter.

The Elegant Inkstones and Artful Calligraphy in Modern Korea: The Catalogue of Three Inkstones Studio (三硯齋硯譜, Samyŏnjae yŏnbo)

This study investigates The Catalogue of Three Inkstones Studio (三硯齋硯譜, &lt;i&gt;Samyŏnjae yŏnbo&lt;/i&gt;), compiled by Pak Yŏngch’ŏl (朴榮喆, 1879~1939), a prominent Korean bureaucrat, businessman, and collector during the Japanese colonial period. The catalogue centers on three inkstones reputedly associated with notable Chinese and Korean scholars. Pak Yŏngch’ŏl, despite his modernist and enlightenment leanings, was deeply rooted in classical Chinese culture and used his collection to assert his cultural and intellectual identity.&lt;/br&gt;The study examines the cultural significance of inkstones, their role in forging social ties, and the strategic utilization of the past by modern intellectuals for self-fashioning. The first inkstone is notable for its inscriptions by Dong Qichang (董其昌), Weng Fanggang (翁方綱), and Kim Chŏnghŭi (金正喜), illustrating the cultural interconnectedness between Chinese and Korean scholars. The second inkstone, crafted by the eighteenth-century scholar Chŏng Ch’ŏlcho (鄭喆祚), is explored in terms of the craftsmanship and social networking practices of the late Chosŏn era. The third inkstone features a poem by Shen Rujin (沈汝瑾) and showcases the transnational cultural exchanges between China, Korea, and Japan.&lt;/br&gt;Pak Yŏngch’ŏl’s collection practices and the subsequent compilation of the catalogue reflect his efforts to align himself with the cultural heritage of both China and Korea, while also embracing modernization under Japanese influence. The catalogue not only serves as a historical compendium but also as a reimagined past that aligns with the cultural aspirations of subsequent generations. Through a detailed analysis of the inkstones and their associated texts, the study provides a micro-historical perspective on East Asian intellectual history and material culture, revealing the intricate interplay between cultural memory, networks, and craftsmanship.

Enlivening Wit

The birth of such innovative newspapers as The Tatler and The Spectator enables the readers to observe and join the public debate through a new intellectual lens. The use of Latin epigrams opening every issue of The Spectator emphasizes not only the close interest in classical literature and culture, but also the communicative strategy focused on the celebration of wit through the moral paradigms of ancient Roman poets, at a time of the rise of modern public opinion. The poetical and rhetorical choices of Joseph Addison and Richard Steele, along with other Latin poetry enthusiasts such as Jonathan Swift and Alexander Pope, bitingly denounce social hypocrisies and contradictions through parody and mockery. Their purpose was to rebut conventional behavior through the permeating force of Enlightenment wit inspired by cleverness, satire and sarcasm.

Mouse vascularized adipose spheroids: an organotypic model for thermogenic adipocytes.

Adipose tissues, particularly beige and brown adipose tissue, play crucial roles in energy metabolism. Brown adipose tissues' thermogenic capacity and the appearance of beige cells within white adipose tissue have spurred interest in their metabolic impact and therapeutic potential. Brown and beige fat cells, activated by environmental factors like cold exposure or by pharmacology, share metabolic mechanisms that drive non-shivering thermogenesis. Understanding these two cell types requires advanced, yet broadly applicable in vitro models that reflect the complex microenvironment and vasculature of adipose tissues. Here we present mouse vascularized adipose spheroids of the stromal vascular microenvironment from inguinal white adipose tissue, a tissue with 'beiging' capacity in mice and humans. We show that adding a scaffold improves vascular sprouting, enhances spheroid growth, and upregulates adipogenic markers, thus reflecting increased adipocyte maturity. Transcriptional profiling via RNA sequencing revealed distinct metabolic pathways upregulated in our vascularized adipose spheroids, with increased expression of genes involved in glucose metabolism, lipid metabolism, and thermogenesis. Functional assessment demonstrated increased oxygen consumption in vascularized adipose spheroids compared to classical 2D cultures, which was enhanced by β-adrenergic receptor stimulation correlating with elevated β-adrenergic receptor expression. Moreover, stimulation with the naturally occurring adipokine, FGF21, induced Ucp1 mRNA expression in the vascularized adipose spheroids. In conclusion, vascularized inguinal white adipose tissue spheroids provide a physiologically relevant platform to study how the stromal vascular microenvironment shapes adipocyte responses and influence activated thermogenesis in beige adipocytes.

Renaissance culture, emblems, and interdisciplinary research: the reception of Alciato in Coimbra☆

AbstractBearing in mind that emblem books were a manifestation of humanistic culture and its natural interdisciplinary, this paper discusses how the early reception of Alciato's Emblemata in Coimbra (Portugal) had an impact on artists, literary authors, jurists, and Jesuit teachers. This emblem book attracted readers from diverse fields of knowledge, and in this essay, special attention is drawn to the role played by Sebastian Stockhamer's commentaries on the Emblemata, published by the Lyonnaise printer, Jean de Tournes, in 1556. From the analysis of textual evidence, this contribution aims to demonstrate that Stockhamer's work was built upon an interdisciplinary method. His example provides a very strong argument for considering Coimbra as pioneering in the early history of the reception of Alciato's work across Europe. This study case embraces therefore a wider range of engagement, involving cultural studies, literature, arts, legal history, educational science, philosophy, and classical culture, among others.

In Search of Nature and Consciousness in Andrei Bialobotskii’s Pentateugum: Classical Echoes and Modern Impulses

Through close reading and reference to the philosophies of the time, this paper demonstrates how the long poem Pentateugum (1690s) by Andrei Bialobotskii (ca. 1640-1720) offers us a dynamic picture of the tensions —between Classical and Orthodox cultures and between religious and secularized literatures—leading to the East Slavic Enlightenment. According to Andrei Bialobotskii, culture is vain because such is human knowledge. Simultaneously, culture triumphs because, through it, the subject acquires and expresses their consciousness in a summa that comprises the whole history of humankind. In this context, Classical culture provides a model for writing techniques and functions as the highest instance of punished vanity, while expressing the harmony between nature and the individual.

A feasibility study of controlled human infection with intradermal Bacillus Calmette-Guérin (BCG) injection: Pilot BCG controlled human infection model.

Tuberculosis (TB) caused 1.5 million deaths in 2020, making it the leading infectious killer after COVID-19. Bacille Calmette-Guerin (BCG) is the only licensed vaccine against TB but has sub-optimal efficacy against pulmonary TB and reduced effectiveness in regions close to the equator with high burden. Efforts to find novel vaccines are hampered due to the need for large-scale, prolonged, and costly clinical trials. Controlled human infection models (CHIMs) for TB may be used to accelerate vaccine development by ensuring only the most promising vaccine candidates are selected for phase 3 trials, but it is not currently possible to give participants Mycobacterium tuberculosis as a challenge agent. This study aims to replicate and refine an established BCG CHIM at the Liverpool School of Tropical Medicine. Participants will receive an intradermal injection with licensed BCG vaccine (Statens Serum Institut strain). In phase A, participants will undergo punch biopsy two weeks after administration, paired with minimally invasive methods of skin sampling (skin swab, microbiopsy, skin scrape). BCG detection by classical culture and molecular methods will be compared between these techniques and gold standard punch biopsy. Techniques meeting our pre-defined sensitivity and specificity criteria will be applied in Phase B to longitudinally assess intradermal BCG growth two, seven and fourteen days after administration. We will also measure compartmental immune responses in skin, blood and respiratory mucosa in Phase B. This feasibility study will transfer and refine an existing and safe model of BCG controlled human infection. Longitudinal BCG quantification has the potential to increase model sensitivity to detect vaccine and therapeutic responses. If successful, we aim to transfer the model to Malawi in future studies, a setting with endemic TB disease, to accelerate development of vaccines and therapeutics relevant for underserved populations who stand to benefit the most. Registration: ISRCTN: ISRCTN94098600 and ClinicalTrials.gov: NCT05820594.

Investigation of Quinolone Residues and Total Aerobic Mesophilic Bacteria in Some Poultry Meat and Chicken Eggs

The presence of quinolones in poultry meat and eggs and the determination of the total aerobic mesophilic bacteria (TAMB) are essential regarding food safety and public health. In this study, quinolone residues and TAMB counts of chicken meat, turkey meat, and chicken eggs sold in supermarkets in Ankara, Turkey were investigated. For this purpose, a total of 84 food samples (40 chicken meats, 14 turkey meats, and 30 chicken eggs) were obtained from different markets in Ankara, and the quinolone residues in the samples were analyzed by enzyme-linked immunosorbent assay (ELISA) technique. The TAMB counts in the samples were investigated with classical culture methods. As a result, the mean level (±S.E) of positive quinolone residue was found to be 56.69±12.41 µg/kg in turkey meat samples. The presence of quinolone was not found in chicken meat samples. The mean levels of TAMB counts of the chicken and turkey meat samples were found 4.87±0.07 and 4.44±0.09 log CFU/g, respectively. Quinolone residues were not detected in chicken egg samples, and TAMB count was also below the detection limit (&lt;102 CFU/g) in the contents of chicken egg samples. Monitoring quinolone residues in poultry meat is important in terms of public health and product quality.

Research progress on the detection of foodborne pathogens based on aptamer recognition.

Foodborne diseases caused by bacterial contamination are a serious threat to food safety and human health. The classical plate culture method has the problems of long detection cycle, low sensitivity and specificity, and complicated operation, which cannot meet the growing demand for rapid quantitative detection of pathogenic bacteria. The frequent outbreak of foodborne diseases has put forward higher requirements for rapid and simple detection technology of foodborne pathogens. Aptamer is a kind of oligonucleotide fragment that can recognize targets with the advantages of high affinity and good specificity. The target can be range from proteins, small molecules, cells bacteria, and even viruses. Herein, the latest advances in sensitive and rapid detection of foodborne pathogens based on aptamer recognition was reviewed. Special attention has been paid to the obtained sequences of aptamers to various foodborne pathogens, the optimization of sequences, and the mechanism of aptamer recognition. Then, the research progress of biosensors for the detection of pathogenic bacteria based on aptamer recognition were summarized. Some challenges and prospects for the detection of foodborne pathogens based on aptamer recognition were prospected. In summary, with the further deepening of aptamer research and improvement of detection technology, aptamer-based recognition can meet the needs of rapid, sensitive, and accurate detection in practical applications.

Transmission of Non-Legacy Classics and Renewal of Culture: A Survey and Analysis of Woodblock Prints in Zhuxian Town

Henan Zhuxianzhen woodblock prints is the originator of Chinese woodblock prints and a treasure of Chinese classic traditional culture, which not only carries the reverence and praise of generations of Kaifeng craftsmen for woodblock prints, but also carries the deep historical memory and rich cultural connotation of the Chinese nation. However, the protection and inheritance of non-heritage work is not an overnight thing, through the publicity and promotion of Zhuxianzhen woodblock prints, can enhance public awareness and understanding of non-heritage culture, and improve the cultural consciousness and cultural self-confidence of the whole society. This paper will mainly focus on the inheritance and protection of Zhuxianzhen woodblock prints and the current situation of the cultural development of the market situation of the two aspects of the analysis and research, and at the same time for the problems existing at this stage of the proposal has a strong focus on the resolution of the measures.

Characterization of 35 novel NR5A1/SF-1 variants identified in individuals with atypical sexual development: The SF1next study.

Steroidogenic factor 1 (NR5A1/SF-1) is a nuclear receptor that regulates sex development, steroidogenesis and reproduction. Genetic variants in NR5A1/SF-1 are common among differences of sex development (DSD) and associate with a wide range of phenotypes, but their pathogenic mechanisms remain unclear. Novel, likely disease-causing NR5A1/SF-1 variants from the SF1next cohort of individuals with DSD were characterized to elucidate their pathogenic effect. Different in silico tools were used to predict the impact of novel NR5A1/SF-1 variants on protein function. An extensive literature review was conducted to compare and select the best functional studies for testing the pathogenic effect of the variants in a classic cell culture model. The missense NR5A1/SF-1 variants were tested on the promoter luciferase reporter vector -152CYP11A1_pGL3 in HEK293T cells and assessed for their cytoplasmic/nuclear localization by Western blot. Thirty-five novel NR5A1/SF-1 variants were identified in the SF1next cohort. Seventeen missense NR5A1/SF-1 variants were functionally tested. Transactivation assays showed reduced activity for 40% of the variants located in the DNA binding domain and variable activity for variants located elsewhere. Translocation assessment revealed three variants (3/17) with affected nuclear translocation. No clear genotype-phenotype, structure-function correlation was found. Genetic analyses and functional assays do not explain the observed wide phenotype of individuals with these novel NR5A1/SF-1 variants. In nine individuals, additional likely disease-causing variants in other genes were found, strengthening the hypothesis that the broad phenotype of DSD associated with NR5A1/SF-1 variants may be caused by an oligogenic mechanism.

Assessment of the Microbiological Quality and Effect of Public Health of Ready-to-Eat Salad Samples in Isparta.

Salmonella spp. and Citrobacter spp. are among the microorganisms causing important foodborne outbreaks. In this study, it was tried to determine the presence and rate of Salmonella spp. and Citrobacter spp. in salad samples collected from certain regions of province of Isparta in Türkiye. A total of 50 salad samples were analyzed. Classical culture technique was used for microbiological analysis of salad samples. Suspected isolates obtained were identified using the VITEK-2 system. Although no negative visual changes were observed in the salad samples used in the study, it was determined that the number of Gram-negative microorganisms was very high and six salad samples were not suitable for public health. In 50 salad samples, 2% Salmonella and 4% Citrobacter freundii were detected. In addition, it was determined that the Salmonella strain isolated from the salad sample was resistant to three different antibiotics and Citrobacter was resistant to two different antibiotics. Salmonella spp. and Citrobacter spp. are considered very dangerous to public health because they are associated with foodborne outbreaks and can develop antibiotic resistance very quickly. Salad producers should try to reduce the possibility of microbial contamination by using different technologies.

A Molecular Approach for Detecting Bacteria and Fungi in Healthcare Environment Aerosols: A Systematic Review.

Molecular methods have become integral to microbiological research for microbial identification. This literature review focuses on the application of molecular methods in examining airborne bacteria and fungi in healthcare facilities. In January 2024, a comprehensive electronic search was carried out in esteemed databases including PubMed, Web of Science, and Scopus, employing carefully selected keywords such as ((bacteria) OR (virus) OR (fungi)) AND (aerosol) AND ((hospital) OR (healthcare) OR (dental office)) AND ((molecular) OR (PCR) OR (NGS) OR (RNA) OR (DNA) OR (metagenomic) OR (microarray)), following the PRISMA protocol. The review specifically targets healthcare environments with elevated concentrations of pathogenic bacteria. A total of 487 articles were initially identified, but only 13 met the inclusion criteria and were included in the review. The study disclosed that the prevalent molecular methodology for appraising aerosol quality encompassed the utilization of the PCR method, incorporating either 16S rRNA (bacteria) or 18S rRNA (fungi) amplification techniques. Notably, five diverse molecular techniques, specifically PFGE, DGGE, SBT, LAMP, and DNA hybridization methods, were implemented in five distinct studies. These molecular tests exhibited superior capabilities compared to traditional bacterial and fungal cultures, providing precise strain identification. Additionally, the molecular methods allowed the detection of gene sequences associated with antibiotic resistance. In conclusion, molecular testing offers significant advantages over classical microbiological culture, providing more comprehensive information.