Cryptosporidiosis is one of the most important zoonosis diseases distributed worldwide causes severe diarrhea in animals and is lethal in quails. This study detected Cryptosporidium spp. in quails in Baghdad province Iraq by flotation and staining methods and compared these results with PCR results from the previous study. This research was carried out from January 2022 to September 2022. Out of 180 quails (Coturnix coturnix) in total were investigated. The overall infection rate of Cryptosporidium spp. in quails was 23.9% (43/180). Young quail had a greater infection rate of 44.2% (19/43) while adult quails had a lower infection rate of 17.2% (24/137). Finding from the research revealed that there were no significant differences between males and females and highly significant differences among the months of study with the highest infection rate being 45% (9/20) in April, and the lowest infection rate being 10% in June and July. This disease was presented in birds and birds act as a mechanical carrier of Cryptosporidium spp. between a conventional acid-fast staining procedure and PCR test, PCR can not only detect Cryptosporidium but is also able to differentiate between what appear to be host-adapted genotypes of the parasite with high sensitivity and specificity.