Circular Imaging Research Articles

Probability weighted four-point arc imaging algorithm for time-reversed lamb wave damage detection

Damage imaging based on scattering signals of ultrasonic Lamb waves in plate structure is considered as one of the most effective ways for structural health monitoring area. To improve location accuracy and reduce the impact of artifacts, a probability weighted four-point arc imaging algorithm for time reversal Lamb wave damage detection is proposed in this paper. By taking the defect as a secondary wave source, the four-point arc positioning method is used to calculate the propagation time of the signal from transducer to defect. And the amplitude of damage signal corresponding to the time of flight is used for imaging. In order to eliminate the artifacts, a damage probability weighting is combined with four-point circular arc imaging algorithm. The effectiveness of the proposed method is experimentally verified in aluminum plate. Experimental results indicate that damage location accuracy and imaging quality has been improved in both single-flaw and double-flaw samples compared with conventional delay-and-sum method.

Real-Time Monitoring of Hierarchical Self-Assembly and Induction of Circularly Polarized Luminescence from Achiral Luminogens.

Constructing artificial helical structures through hierarchical self-assembly and exploring the underlying mechanism are important, and they help gain insight from the structures, processes, and functions from the biological helices and facilitate the development of material science and nanotechnology. Herein, the two enantiomers of chiral Au(I) complexes ( S)-1 and ( R)-1 were synthesized, and they exhibited impressive spontaneous hierarchical self-assembly transitions from vesicles to helical fibers. An impressive chirality inversion and amplification was accompanied by the assembly transition, as elucidated by the results of in situ and time-dependent circular dichroism spectroscopy and scanning electron microscope imaging. The two enantiomers could serve as ideal chiral templates to co-assemble with other achiral luminogens to efficiently induce the resulting co-assembly systems to show circularly polarized luminescence (CPL). Our work has provided a simple but efficient way to explore the sophisticated self-assembly process and presented a facile and effective strategy to fabricate architectures with CPL properties.

Electronic circular dichroism imaging (CDi) maps local aggregation modes in thin films of chiral oligothiophenes

A detailed investigation of the circular dichroism imaging (CDi) technique on thin films of a chiral 1,4-dialkoxyphenylene-based oligothiophene with outstanding chiroptical features revealed the primary role of local supramolecular structures.

Resolution-Based Analysis for Optimizing Subaperture Measurements in Circular SAR Imaging

Circular geometries are often encountered in nondestructive testing and evaluation applications. Circular synthetic aperture radar imaging technique, capable of producing high-resolution images, has great utility for this purpose. However, in many such applications, scanning only a portion of the circular geometry (i.e., scanning domain), called the subaperture, may only be possible or sufficient for a particular inspection need. Consequently, in subaperture imaging, for an imaging aspect angle and aspect angle measurement interval, we must determine the expected image resolution. In this paper, for an arbitrary area of interest, we analyze the image spatial resolution obtained from a generally defined aspect angle and aspect angle measurement interval. Using resolution as the criteria, we use this method to determine the boundary between small and large subapertures, which also show to produce markedly different range and cross-range resolutions as a function of aspect angle and aspect angle measurement interval. Moreover, the optimum aspect angle and aspect angle measurement interval for this fixed area of interest are defined based on these analyses. Finally, the results of these analyses and images obtained from simulations are compared and corroborated with relevant experimental results at X-band (8.2–12.4 GHz).

Circular intensity differential scattering (CIDS) scanning microscopy to image chromatin-DNA nuclear organization

Circular dichroism imaging has proved a powerful and simple method for extracting information on chiral molecules without specific fluorescent labels. Numerous mathematical models show that outside the absorption band, the circular dichroism signal comes from the scattering interaction and brings additional information about the organization of biopolymers. With this article, we propose a fast method to control the polarization states without moving parts, by means of a photoelastic modulator. We implemented the technique on a modified commercial confocal microscope realizing a multimodal configuration. We demonstrate its imaging capabilities by studying the organization of chromatin DNA inside isolated cell nuclei.

Group geometric calibration and rectification for circular multi-camera imaging system

Calibration on multi-camera imaging system in circular arrangement is a challenge task, because the preliminary shared feature points are absent among opposite viewpoints in the calibration procedure. In this paper, we propose a scheme of group geometric calibration and ideal rectification for circular multi-camera imaging system. In this scheme, a three-dimensional (3D) calibration object with new patterns is firstly designed to extract the extrinsic parameters. After that, the ideal camera projection matrix is estimated based on these parameters. Finally, we extend our scheme from static to dynamic scenario. In the experiments, our method has a superior performance over state-of-the-art calibration methods. Furthermore, we verify our scheme by application of 3D modeling and improvements can also be found. These results demonstrate that our method performance on such imaging system is applicable for future interactive visual applications.

The sensitive detection of ODAM by using sandwich-type biosensors with a cognate pair of aptamers for the early diagnosis of periodontal disease

This research aims to develop biosensors which could diagnose periodontal diseases in early phases and predict the illness stage of patients, in order to give them adequate treatment timely. Human odontogenic ameloblast-associated protein (ODAM) is considered to be a potential biomarker for periodontal diseases, based on high correlation between the level of ODAM in gingival crevicular fluid (GCF) and the degree of periodontitis. Many aptamers, including a cognate pair of aptamers which can bind to the different sites of ODAM, were successfully screened in a very stringent condition employing saliva as a counter target through the graphene oxide-based systemic evolution of ligands by exponential enrichment (GO-SELEX). For the characterization of the aptamer candidates, GO-based fluorescence resonance energy transfer (GO-FRET) and surface plasmon resonance (SPR) assays were conducted. The sandwich-type binding of a cognate pair of aptamers to ODAM was additionally confirmed by employing circular dichroism (CD) and magnetic beads-based fluorescence imaging methods. The resulting cognate pair of aptamers, OD64 and OD35, were found to have their dissociation constant (Kd), 47.71 nM and 51.36 nM, respectively. The minimum detectable concentrations of a sandwich-type SPR biosensor were found to be 0.24 nM and 1.63 nM, respectively, for both buffered and saliva samples. Finally, using this cognate pair of aptamers, a sandwich-type lateral flow strip biosensor was successfully realized. This research shows the potential for implementation of a cognate pair of aptamers on point-of-care biosensors which enables simple, rapid, and non-invasive saliva-based diagnosis of periodontal-related diseases that can overcome current diagnostic methods and improve health care system.

Circular orientation fluorescence emitter imaging (COFEI) of rotational motion of motor proteins

Single-molecule fluorescence polarization technique has been utilized to detect structural changes in biomolecules and intermolecular interactions. Here we developed a single-molecule fluorescence polarization measurement system, named circular orientation fluorescence emitter imaging (COFEI), in which a ring pattern of an acquired fluorescent image (COFEI image) represents an orientation of a polarization and a polarization factor. Rotation and pattern change of the COFEI image allow us to find changes in the polarization by eye and further values of the parameters of a polarization are determined by simple image analysis with high accuracy. We validated its potential applications of COFEI by three assays: 1) Detection of stepwise rotation of F1-ATPase via single quantum nanorod attached to the rotary shaft γ; 2) Visualization of binding of fluorescent ATP analog to the catalytic subunit in F1-ATPase; and 3) Association and dissociation of one head of dimeric kinesin-1 on the microtubule during its processive movement through single bifunctional fluorescent probes attached to the head. These results indicate that the COFEI provides us the advantages of the user-friendly measurement system and persuasive data presentations.

Reflective scanning imaging based on a fast terahertz photodetector

The fast detection of terahertz radiation is recognized as a key technology of terahertz imaging systems. We realize a terahertz imaging system employing a terahertz quantum-well photodetector (QWP) and a terahertz quantum cascade laser (QCL). The detector can rapidly detect the 4.3THz light generated from a pulsed electrically-pump terahertz QCL, which is used as the terahertz source of the imaging system. The object is placed on a rotary scanning platform to realize fast scanning. A practical detection method of the terahertz pulse signal is employed to extract the amplitude information from the terahertz signal and improve the signal-to-noise ratio (SNR) of the system. The electrical and optical performances of the fast terahertz QWP are characterized, and the results show that the terahertz QWP can completely meet the fast detection requirement of the terahertz imaging system. The terahertz images of some invisible objects are obtained with a high contrast within 5 s. The imaging system owns a resolution of 0.3 mm and a circular imaging region with a diameter of 100 mm. An image processing algorithm is applied in this system to solve the noise problem and improve the quality of the images. The imaging results indicate that terahertz QWP has a good application prospect in nondestructive inspection applications and the research of the fast physical or chemistry process.

Near-infrared Polarimetry of the Outflow Source AFGL 6366S: Detection of Circular Polarization

Abstract We have carried out near-infrared circular and linear imaging polarimetry of the AFGL 6366S region. There is one large infrared reflection nebula associated with the AFGL 6366S cluster and one small infrared reflection nebula associated with AFGL 6366S NE. Prominent and extended polarized nebulosities over the AFGL 6366S cluster field are found to be composed of several components and local nebula peaks, and those nebulosities are illuminated by at least three sources, which is roughly consistent with a previous study. However, the detailed linear polarization patterns and their degrees differ from the earlier study. The brightest regions of the nebulae are illuminated by the IRAS/WISE source. In addition, we report the first detection of circular polarization (CP) in the reflection nebula associated with AFGL 6366S. The CP is as large as approximately 4% in the K s band, and the maximum CP extent is approximately 0.45 pc, which is comparable to that for the largest CP regions known to date, such as Orion and Mon R2, although the CP degrees are much smaller. The CP pattern is mostly quadrupolar, and its morphology resembles the shape of the C18O dense core. Therefore, the CP region is probably illuminated by the IRAS/WISE source and its polarization is amplified by the dichroic absorption of the dense core associated with the cluster. This is the ninth source whose degrees of CPs are measured to be greater than 3%, suggesting that large and extended infrared CP regions are common among mid- to high-mass young stellar objects.

High-efficiency chiral meta-lens

We present here a compact metasurface lens element that enables simultaneous and spatially separated imaging of light of opposite circular polarization states. The design overcomes a limitation of previous chiral lenses reliant on the traditional geometric phase approach by allowing for independent focusing of both circular polarizations without a 50% efficiency trade-off. We demonstrate circular polarization-dependent imaging at visible wavelengths with polarization contrast greater than 20dB and efficiencies as high as 70%.

The Application of an Ultrasound Tomography Algorithm in a Novel Ring 3D Ultrasound Imaging System.

Currently, breast cancer is one of the most common cancers in women all over the world. A novel 3D breast ultrasound imaging ring system using the linear array transducer is proposed to decrease costs, reduce processing difficulties, and improve patient comfort as compared to modern day breast screening systems. The 1 × 128 Piezoelectric Micromachined Ultrasonic Transducer (PMUT) linear array is placed 90 degrees cross-vertically. The transducer surrounds the mammary gland, which allows for non-contact detection. Once the experimental platform is built, the breast model is placed through the electric rotary table opening and into a water tank that is at a constant temperature of 32 °C. The electric rotary table performs a 360° scan either automatically or mechanically. Pulse echo signals are captured through a circular scanning method at discrete angles. Subsequently, an ultrasonic tomography algorithm is designed, and a horizontal slice imaging is realized. The experimental results indicate that the preliminary detection of mass is realized by using this ring system. Circular scanning imaging is obtained by using a rotatable linear array instead of a cylindrical array, which allows the size and location of the mass to be recognized. The resolution of breast imaging is improved through the adjustment of the angle interval (>0.05°) and multiple slices are gained through different transducer array elements (1 × 128). These results validate the feasibility of the system design as well as the algorithm, and encourage us to implement our concept with a clinical study in the future.

Implementation of the inverse circular radon transform-based imaging approach for breast cancer screening

Implementation of the inverse circular radon transform-based imaging approach for breast cancer screening

航天相机环扫成像模式设计

In order to meet the needs of wide area search using agile satellite, the aerospace camera circular scanning imaging model was designed. Based on analyzing the principle of the circular scanning imaging, the optimal ground track was designed and the relationship between the critical spin speed of the satellite and the orbital velocity, the critical circular scanning coefficient, the overlap rate was analyzed, the circular scanning model was established. At the same time, the exposure time, frame rate and other imaging parameters were determined based on the ground track design. The imaging model by STK software was simulated, and the imaging geometric parameters were analyzed. The result shows that when the orbital height H is 500 km, the pixel size a is 4 m, the focal length f is 1 m and the axial pixel number M is 50 000, the ground sample distance and swath width increase gradually with the increase of the camera inclination , when equals to 10, 20, 30, 40, the swath width raises to 1.96, 3.10, 4.58, 6.85 times compared with nadir imaging.

The processes of α-synuclein amyloid protein complexes involved in the pathogenesis of Parkinson's disease

To analyze interactions between α-synuclein (αS) protein and lipids using biophysical methods. Recombinant α-synuclein synthesized in prokaryotic cells was used. To characterize the interaction of αS with negatively charged vesicles of DOPS (1,2-dioleoyl-sn-glycero-3-phospho-L-serine, sodium salt) and DOPG (1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol), sodium salt) and the consequences of such interactions on αS amyloid formation, combined circular dichroism, fluorescence and imaging methods in vitro were applied. Lipid head-group chemistry modulates αS interactions and also affects amyloid fiber formation. Pre-formed αS oligomers, typically present in a small amount in the αS starting material, acted as templates for linear growth of anomalous amyloid fibers in the presence of vesicles. At the same time, the remaining αS monomers were restricted from vesicle-mediated nucleation of amyloid fibers. Although not a dominant process in bulk experiments, this hidden αS aggregation pathway may be of importance in vivo.

Photographic zoom fisheye lens design for DSLR cameras

Photographic fisheye lenses with fixed focal length for cameras with different sensor formats have been well developed for decades. However, photographic fisheye lenses with variable focal length are rare on the market due in part to the greater design difficulty. This paper presents a large aperture zoom fisheye lens for DSLR cameras that produces both circular and diagonal fisheye imaging for 35-mm sensors and diagonal fisheye imaging for APS-C sensors. The history and optical characteristics of fisheye lenses are briefly reviewed. Then, a 9.2- to 16.1-mm F/2.8 to F/3.5 zoom fisheye lens design is presented, including the design approach and aberration control. Image quality and tolerance performance analysis for this lens are also presented.

An improvement method for circular synthetic aperture radar imaging

ABSTRACTCircular synthetic aperture radar (CSAR) is the imaging mode when the radar moves along a circular path and the observed area is always covered by the wave beam. It is different from traditional SAR modes (strip-map SAR and spotlight SAR) and has potential advantages such as 360° observation, target recognition, and three-dimensional reconstruction. According to the imaging processing of CSAR, motion error is an important issue affecting the CSAR image quality, but the motion compensatio n (MOCO) method for CSAR is underdeveloped. Accordingly, with detailed analysis the motion error model is established and a data-driven MOCO flow chart for CSAR is proposed. The real CSAR data are used to verify the proposed method.

Circular Dichroism Imaging: Mapping the Local Supramolecular Order in Thin Films of Chiral Functional Polymers

We introduce the concept of circular dichroism (CD) imaging CDi, which is made possible by the use of the highly collimated light beam of synchrotron radiation (SR) only available at Diamond Light Source B23 beamline for surface scanning of solid samples. At the moment, the space resolution is about 1 mm2, but there are prospects to increase it to at least 0.1 mm2 in the near future. We apply SR-CDi to thin films of conjugated polymers, which give rise to large chiral aggregates. Electronic CD is able to reveal and characterize these supramolecular structures; thus, SR-CDi provides the ultimate complement to microscopy for investigating local domains with different molecular order and to fill a gap between a purely morphological knowledge and an intimate insight into the molecular level. As a corollary of this proof-of-concept work, we showed that such a new technique may provide rational and successful protocols to obtain homogeneous active layers for the fabrication of optoelectronic devices.

Unraveling amyloid formation paths of Parkinson's disease protein α-synuclein triggered by anionic vesicles.

Amyloid formation of the synaptic brain protein α-synuclein (αS) is related to degeneration of dopaminergic neurons in Parkinson's disease patients. αS is thought to function in vesicle transport and fusion and it binds strongly to negatively charged vesicles in vitro. Here we combined circular dichroism, fluorescence and imaging methods in vitro to characterize the interaction of αS with negatively charged vesicles of DOPS (1,2-dioleoyl-sn-glycero-3-phospho-L-serine, sodium salt) and DOPG (1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol), sodium salt) and the consequences of such interactions on αS amyloid formation. We found that lipid head-group chemistry modulates αS interactions and also affects amyloid fiber formation. During the course of the experiments, we made the unexpected discovery that pre-formed αS oligomers, typically present in a small amount in the αS starting material, acted as templates for linear growth of anomalous amyloid fibers in the presence of vesicles. At the same time, the remaining αS monomers were restricted from vesicle-mediated nucleation of amyloid fibers. Although not a dominant process in bulk experiments, this hidden αS aggregation pathway may be of importance in vivo.

Hybrid Helix Metamaterials for Giant and Ultrawide Circular Dichroism

Circular polarization-resolved imaging and spectroscopy have versatile applications ranging from astronomy and biological and biomedical studies to remote sensing. To achieve real-time imagery with high contrast and rich information, integratable circular polarizers with high extinction ratios, broad operation bandwidths, and low insertion loss are urgently desired, while to our knowledge no structures proposed previously can balance these three vital parameters. In this paper, a concept of a hybrid helix metamaterial has been proposed to achieve ultrawide and giant circular dichroism with low insertion loss simultaneously. By coupling the localized and delocalized resonant modes, a broad and strong blocking band for unselected polarization can be realized with a pencil-like configuration. A giant extinction ratio of 5.9 × 105 can be obtained with a six-pitch hybrid single-helix array, which is almost 7 times larger than the best result reported by Behera et al. in 2015. A very high average extinction rat...