The use of the Pressure Sensitive Paint (PSP) measurement method allows for obtaining dense, completely adiabatic film cooling effectiveness (η) results. In order to adapt to the increasing trend of high-speed and complexity in the application scenarios of film cooling, the pressure deviation-induced film cooling effectiveness deviation in PSP measurements needs to be quantified and corrected. Initially, two different jets of specific materials were injected into a dual-hole flat plate test piece. The pressure difference at corresponding positions of the two jet flows was measured to demonstrate that, compared to air, a ternary oxygen mixed gas better simulated the pressure field of the foreign gas. This illustrates the feasibility of the Test3-OM correction method. Building upon this, the influence of pressure deviation on the accuracy of measurement results was quantitatively evaluated in various scenarios, including a flat circular hole, film holes with a compound angle, a converging slot hole, and film holes at three positions on the blade. The results indicate that the pressure deviation-induced η deviation is non-negligible under conditions of high blowing ratios in most positions. Particularly in the low η region, there is a higher sensitivity to pressure deviation. Generally, there is a negative deviation region near the hole downstream, and a positive deviation region slightly downstream. The generation of deviations stems from variations in density, resulting in different flow patterns of the jet near the wall surface.