The present study was carried out to evaluate the in vitro antimicrobial and antioxidant activity of Emblica officinalis juice powder. The antimicrobial activity was assessed against gram positive and gram negative bacteria namely E.coli, Pseudomonas fluoresces, Bacillus subtilis, S.aureus, and fungi namely Aspergillus niger, Penicillium chrysogenum, Alternaria alternata, and Fusarium spp by agar well diffusion method. The antioxidant activity of the powder was determined in vitro using hydrogen peroxide scavenging activity method. The amount of total phenolic content was also determined by Folin Ciocalteau method. The result of the study revealed antibacterial and antioxidant activity. The Emblica officinalis powder showed no antifungal activity against the tested strains. The activity was found to be concentration dependent. The results of present study conclude that Emblica officinalis juice powder possess high antibacterial and antioxidant activity and can be further explored for the isolation of its bioactive compound.