Cicer Arientinum Research Articles

Path analysis studies for yield and yield attributing characters in chickpea (Cicer arientinum L.) germplasm

The path co-efficient analysis in chickpea indicated that the characters viz., 100 seed weight, number of pods per plant, number of secondary branches per plant, days to 50% flowering, harvest index and number of primary branches per plant on seed yield were the major yield contributing traits in chickpea. Therefore, the selection of genotypes based on these characters as a selection criterion would help to improve the seed yield potential of chickpea.

Genetic diversity studies for yield attributing characters in chickpea (Cicer arientinum L.) germplasm and their clusters

Genetic diversity is a prerequisite for undertaking any crop breeding programe for the development of high yielding crop varieties to enhance the national food production for the food security. Protein is an important component in the human diet, which is obtained for the vegetarian people from the pulses. Chickpea is an important source of protein for the Indian and African population. Therefore, breeding for high yielding chickpea varieties is a continuous process whose success is dependent on the availability of genetic diversity in the chickpea germplasm. In the present studies forty one genotypes of chickpeas were grouped into twelve clusters. The cluster I was with the highest number of genotypes (23) followed by cluster IV (09), II , III, V, VI, VII, VIII, IX, X, XI and XII which had only 1 genotype in each cluster, respectively.The intra cluster distance (D2) ranges from 68.72 to 90.06 while the inter cluster distance (D2) ranges from 49.00 to 1217.31. The maximum inter-cluster distance (D2 = 1217.31) was observed between cluster VI and cluster XII followed by cluster XII and VIII (D2 = 1069.94), cluster VI and cluster X (D2 = 962.24) and cluster V and cluster VI (D2 = 919.90). It was observed that, seed yield per plant (42.20%) contributed highest for divergence followed by plant height (16.83%), 100 seed weight (16.34%), days to maturity (6.83%), number of pods per plant (5.37%), number of primary branches (4.51%), number of secondary branches per plant (3.05%), harvest index (2.20%), number of seeds per pod (2.07%) and days to 50% flowering (0.61%).

Correlation studies for seed yield and its components in chickpea (Cicer arientinum L.) germplasm

The association between seed yield and yield contributing characters was studied by using 41 chickpea genotypes. The characters viz. number of pods per plant, 100 seed weight, harvest index, number of secondary branches per plant and plant height recorded a highly positive significant correlation with seed yield at both genotypic and phenotypic levels. This indicates the simultaneous improvement of these characters through selection.

Studies on the genotypic co-efficient of variation and phenotypic co-efficient of variation in chickpea (Cicer arientinum L.) germplasm for yield-attributing characters

The magnitude of the genotypic co-efficient of variation (GCV) and phenotypic co-efficientof variation (PCV) in chickpea germplasm was observed high for characters like seed yield per plant followed by number of pods per plant, 100 seed weight, plant height, number of secondary branches per plant and number of primary branches per plant. The characteristics viz., seed yield per plant showed high GCV and PCV, moderate heritability and high genetic advance as a per cent of the mean. The characters viz. number of pods per plant, 100 seed weight, harvest index, number of secondary branches per plant and plant height recorded a highly positive significant correlation with seed yield at both genotypic and phenotypic levels. This indicates the simultaneous improvement of these characters through the selection of chickpeas.

Genome-wide association analysis in chickpea landraces and cultivars

Chickpea (Cicer arientinum) is an important leguminous crop, which is widely grown especially in the Near East. In wet weather conditions, the susceptibility of chickpeas to fungal diseases such as Ascochyta blight and Fusarium blight increases. Thus, selection of disease-resistant and early-ripening varieties is critically needed. The present study was conducted to investigate genome associations in 171 samples of chickpea plants, grown in two experimental stations in Krasnodar (Kuban experimental station) and Astrakhan (Astrakhan experimental station), examine relationship between genes and 12 phenotypic traits as well as explore the association between genes and 3 hallmarks of resistance to pathogenes: Fusarium blight, Ascochyta blight and Noctuidae. Variants associated with different phenotypic traits were identified using a genome-wide association study (GWAS).

Silica supported biosynthesized silver nanoparticles as effective adsorbent and photocatalyst for removal of methylene blue from water

The silica supported silver nanoparticles (Ag@SG) were prepared by wet impregnation of silica gel (SG) using different concentrations of biogenic silver nanoparticles (AgNPs; 0.25–5 mM) solution, prepared from Cicer arientinum pod extract. Among them, the Ag@SG composite prepared by using 1.25 mM AgNPs solution (Ag1.25@SG) was found to contain only Ag0 species as nanoparticles, whereas the samples prepared by higher concentrations contained Ag0 as well as Ag+ (silver oxide) species. All the Ag@SG composites including SG, exhibited good adsorption capacity for a cationic dye (methylene blue; MB) in water. However, Ag1.25@SG having only Ag0 species exhibited higher photocatalytic activity for degradation of MB under visible light irradiation as compared to other composites. The study reveals that the plasmonic Ag0 species supported on silica are catalytically more active than Ag+ species. Furthermore, the AgNPs (Ag0) in supported form exhibits much higher (∼5-fold) photocatalytic activity than unsupported AgNPs. The synergistic effect of adsorption property of silica surface and plasmonic photocatalytic activity of AgNPs in the synthesized composites was found to be effective for the removal of MB. In addition, the fine dispersion of AgNPs on internal surface of silica provides better activity for photocatalysis. The study shows that this material is a promising adsorbent as well as photocatalyst for the removal of dyes from water.

Removal of Mercury (II) ions from aqueous solution by adsorption on Cicer arientinum husk: Equilibrium, Kinetic and Mechanism Studies

In the present work, Bengal gram husk (BGH) of low cost, minimally pretreated and locally available abundant agricultural waste material was used as an adsorbent for removal of Hg2+ from aqueous media. The influence of several parameters like effect of pH, adsorbent dosage, initial metal concentration, contact time and presence of interfering ions were studied through batch adsorption studies. The adsorption models of Langmuir and Freundlich were used for for the calculation of isotherm constants. According to the Langmuir model, the maximum adsorption capacity of BGH for Hg (II) ions was found to be 51.85 mg/g. The presence of interfering ions like Mg, Ca, K and Na did not play a role in the removal of Hg (II) ions. The Hg (II) removal by BGH followed pseudo first order rate kinetics. The FTIR spectra and CHN analysis of BGH showed the presence of CH and CO groups on the surface of BGH, which played a significant role in removal of Hg (II). Desorption studies revealed that about 83% of the dye was desorbed at pH 2. The present work showed that the BGH can be applied to remove Hg (II) from wastewater.

Mitigation of hydrocarbon toxicity using bacterial consortium in microcosm environment for agrarian fecundity

Petroleum contamination in the soil has been well emphasized as a toxic and hazardous soil pollution contributing to a significant portion of soil infertility worldwide. In the present study, bacterial consortium CHM1 composed of 5 strains belonging to genera Klebsiella, Pantoea, and Enterobacter was evaluated for hydrocarbon degradation ability in the soil environment, as well as their performance in remediating ecotoxicity and phytotoxicity. Initially, the degradation efficiency (1.98%/day) in the soil environment was evaluated. Scanning Electron Microscopy combined with Energy Dispersive X-ray spectroscopy revealed an increase in nitrogen content by 24.98% and a decrease in carbon content by 22.76% implying an improvement in soil fertility. The Fourier Transform InfraRed spectroscopy and Gas Chromatographic analysis revealed significant depletion of aromatic, cyclic, long aliphatic, and complex acid and ester content of the test soil. Moreover, the quantitative PCR analysis exhibited the non-competitive coexistence of each component of the CHM1 consortium. Different enzymatic assays revealed elevated dehydrogenase and superoxide dismutase activity in the degradation system due to the introduction of CHM1 in the soil microcosm. Vibrio fischeri-assisted ecotoxicity analysis had established the potential of CHM1 to efficiently minimize the ecotoxicity of hydrocarbon contamination. The phytotoxicity analysis was performed using four different plant models viz. Chickpeas (Cicer arientinum), Coriander (Coriandrum sativum), Fenugreek (Trigonella foenum-graecum), and Spinach (Spinacia oleracea) exhibiting CHM1 amendment helped to restore plant germination and growth in hydrocarbon-contaminated soil system efficiently. The promising results from this study indicated the possible application of the bacterial consortium in hydrocarbon-contaminated land management and soil restoration for cultivation or other plantation purposes.

Evaluation of Seed Priming on Growth of Chickpea (Cicer arientinum L.)

The present experiment was conducted during Rabi 2019 at experimental field of Pulses Research unit, Dr. PDKV, Akola to assess the suitable priming treatment for chickpea crop (variety: JAKI-9218). The experiment was laid out in randomize block design in three replication. The morpho-physiological traits viz. germination %, plant height, number branches, dry matter content, number of day required to 50% flowering were taken.. The morpho-physiological i.e. plant height, number of branches, dry matter content, germination % showed significant increased in chickpea crop when seed priming was carried out with potassium nitrate for 4 hours. The numbers of days required to 50% flowering were recorded least in seed priming GA3 for 4 hours. Protein content also recorded significantly higher in seed priming with potassium nitrate for 4 hours. Yield and yield attributes were also significantly increased in seed priming with potassium nitrate for 4 hours. It is concluded that seed priming were with potassium nitrate for 4 hours recorded higher morpho-physiological characters over priming treatment and control.

Isolation and Characterization of Plant Growth Promoting Bacteria from Root Nodules of Cicer arientinum L.

Aims: The aim of the present study is to isolate and characterize root nodulating bacteria from Cicer arientinum L cv. GNG 1958 and assess its plant growth promoting properties. The study is based on previous reports that native symbiotic bacteria may be replaced by better performing strains by corss-inoculation to increase productivity and tolarance to Biotic and abiotic stresses.
 Study Design: Isolation of symbiotic bacteria, its biochemical and molecular characterization and assessment of plant growth promoting property through ammonia production, phosphate solubilization and HCN production.
 Place and Duration of Study: Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan, during 2019-2022.
 Methodology: Root nodulating bacterial strain was isolated from C. arientinum L. cv. GNG 1958 grown at the agricultural fields' located at the Krishi Vigyan Kendra, Banasthali Vidypapith, Rajasthan. Morphological characteristics of the isolates were noted following Bergey’s Manual of Systemic Bacteriology and biochemical tests performed as per standard methods. Molecular characterization of the potential symbiont was performed by PCR of 16s rRNA genes and the PCR product was sequenced. Plant growth promoting property was determined through ammonia production, phosphate solubilization and HCN production.
 Results: Root nodulating bacterial strain was isolated from C. arientinum L.cv. GNG 1958. Out of eighteen isolates, BVCA-58 grew fast and changed the color of BTB dye in media from green to yellow and deep yellow within 2 days of incubation. White colonies were observed when BVCA-58 was inoculated on YEMA media supplemented with Congo red. BVCA-58 has the ability to produce hydrogen cyanide, ammonia and solubilize phosphate. Molecular study of BVCA-58 using 16f-16r and 63f-1244r primers showed 99.89% and 99.62% of similarity with Mesorhizobium jarvisii ATCC-33669 and Mesorhizobium erdmanii USDA-3471 respectively on performing NCBI Blast. BVCA-58 was submitted to NCBI portal as Mesorhizobium sp. strain BVCA-58 with accession nos. OP646813 and OP646810.
 Conclusion: The present study revealed that isolated strain from Cicer arientinum GNG 1958 variety was Mesorhizobium sp. which has potent plant growth promoting traits.

Efffects of Rhizobium Inoculation, Sulphur and Zinc Nutrients on Yield and Yield Componets of Chickpea (Cicer aritienum L.) at Gewane, Northeastern Ethiopia


 The goal of this study was to see how a combination of Rhizobium inoculant, S, and Zn nutrients affected chickpea nodulation and yield (Cicer arientinum L.) at Gewane, northeastern Ethiopia. The experiment included 12 treatments arranged in factorial combination of levels of inoculation (Rhizobium inoculated, un-inoculated), three rates of S (0, 15, 30 kg Sulphur ha-1) and rates of Zn (0, 1.5 kg Zinc ha-1). The treatment becomes laid out in randomized complete block design with 3 replications. Analysis of variance showed that yield and yield associated parameters (main branches, number of pod, quantity of seed, hundred seed weight, grain and straw yield), crop phenology (days to 50% flowering and days to physiological maturity), nodulation scores (nodule amount, nodule volume, nodule dry weight, effective nodule and nodulation score), N and P uptake at both locations had been significantly affected by the treatments. The best (1775.5 kg ha-1) suggest rate of seed yield over locations changed into acquired from blended costs of rhizobium and 30 kg S ha-1 which led to 28 % (389 kg ha-1) growth over the control. Consequently, rhizobium inoculation with rate of 30 kg S ha-1is recommended for chickpea production on the experimental site.

Effect of Pseudomonas fluorescens in manganese uptake by chickpea (Cicer arietinum L.) cultivars infected by root-knot nematodes (Meloidogyne incognita)

Chickpea (Cicer arientinum L.) is one of the most dominant pulse crops in India, which contributes 38 percent of the area and 50 percent production of pulses compare to the total pulse production of India. Chickpea contains protein-2.1%, carbohydrates-61.5%, and fat-4.5% and more iron, calcium and niacin content. The main constrain of chickpea production due to parasitic nematodes (Meloidogyne incognita) is about 14% of total global production in annual yield loss. Pseudomonas fluorescens is a bacterial bio-agent that can help in nematode suppression in chickpea plants. This experiment was conducted to experience the differences, if any, in manganese content concerning chickpea inoculated with M. incognita with a combination of Pseudomonas fluorescens as a bioagent, where different treatments of nematode, bacteria, and chemicals are used sustaining the enhancement of disease resistance in chickpea cultivars RSG 974, GG 5, GNG 2144. The total manganese content of chickpea variety GNG 2144 was found highest in treatment, where only bacteria (P. fluorescens) was inoculated, i.e., 6.44 mg/100g of a root, followed by GG 5, i.e., 5.63 mg/100g of root and RSG 974 was, i.e., 4.14 mg/100g of root respectively. Application of Pseudomonas fluorescence combined or alone gradually increased the manganese concentration in roots of chickpea plants i.e., RSG 974 (4.14 mg/100g), GG 5(5.63 mg/100g), GNG 2144 (6.44 mg/100g) compared to the health check.

Efficacy of Selected Insecticides and Botanicals against the Infestation of Gram Pod Borer, Helicoverpa armigera Hub. on Chickpea, Cicer arientinum L.

The present research was conducted at the Central Research Field of Department of Agricultural Entomology, Sam Higginbottom University of Agriculture Technology and Sciences, Prayagraj during the Rabi season of 2021-22. The treatments used during the study were Metarhizium anisopliae, Beauveria bassiana, Karanj Oil, Neem Oil, Spinosad, Emamectin benzoate + Indoxacarb and Indoxacarb. The results revealed that highest percent reduction in larval population was observed in Emamectin benzoate 5% SG + Indoxacarb 14.5% SC (73.57%) and was most successful in bringing down the larval population. It is then followed by Spinosad 45% SC (65.03%), Indoxacarb 14.5% SC (59.02%), Beauveria bassiana (1x108 cfu) (54.87%), Metarhizium anisopliae (1x108 cfu) (50.92%), Neem Oil 2% (45.81%). While, Karanj Oil 2% prove to be the least effective treatment in reducing the larval population (37.53%).

Bioremediation potential of newly isolated Bacillus albus strain VKDS9 for decolourization and detoxification of biomethanated distillery effluent and its metabolites characterization for environmental sustainability

In India, the environment friendly and sustainable management of colored effluents emanating from alcohol-producing distilleries has become a serious environmental concern. The current study aimed to examine the efficiency of ligninolytic enzyme-producing bacterial strain isolated from ameliorated distillery sludge by enrichment method for its bioremediation and detoxification potential of biomethanated distillery effluent (BMDE). Out of nineteen bacterial isolates isolated from distillery sludge, one strain VKDS9, having higher ability towards the removal of effluent’s color, was identified as Bacillus albus by 16S rRNA analysis. The bacterium showed significant decolourization of effluent up to 83% with subsequent reduction of biochemical oxygen demand (3261 mg/L), chemical oxygen demand (7870.5 mg/L), and other physico-chemical parameters including metallic and non-metallic elements in the presence of glucose (1% w/v) and peptone (0.5% w/v) at optimal temperature 37 °C, pH 8.0, and 180 rpm within 144 h. Further, biodegradation of effluent was confirmed by high performance liquid chromatography analysis that showed appearance/disappearance and reduction of several peaks. Moreover, GC–MS​ analysis of bacterial treated effluent displayed the disappearance of several chemical contaminants and formation of new metabolites which were formed as end products of biodegraded effluent. Furthermore, phytotoxicity of treated and untreated BMDE was evaluated using Cicer arientinum L. seeds revealed the biodetoxification of BMDE after treatment by B. albus strain VKDS9. This study concluded that B. albus strain VKDS9 can be used as an efficient biotechnological tool for remediation of distillery effluent for its harmless dumping into the environment towards sustainable development. • Novel ligninolytic enzyme producing Bacillus albus strain VKDS9 was isolated. • Significant pollutant load along with color removal was achieved by strain VKDS9. • Phytotoxicity​ assessment showed toxicity reduction in treated distillery effluent. • Strain VKDS9 can be used as a promising tool for remediation of distillery effluent.

REMOVAL OF HEAVY METALS AND AGROCHEMICALS RESIDUES THROUGH PLANTS

The present paper presents about removal of heavy metals, agrochemicals residues through natural plants. The removal of heavy metals residues and agrochemical residues with the interception of natural plants is called phytoremediation. The advancement of applied research produced various phytoremediation plants like weed plant, aquatic plant, tree and crops. The natural plant eradicates heavy metals and agrochemicals residues through phytostabilization, rhizodegradation, rhizofilteration, phytodegradation and phytovoltalization process. The terrestrial and aquatic plant eradicates heavy metals and agrochemicals residues from soil and water. Indian mustard removes 3 fold Cd, Pb, Se and Cs radioactive metals and diesel fuel spillage. Oryza sativa L. is cereal crop, removes Cu, Cd, from contaminated soil. Spinacia oleracea (Spinach) is vegetable crop, removes Cd, Cu, Fe, Ni, Pb, Zn and Cr from contaminated soil. Cicer arientinum is pulse crop, eradicates Pb and Cr from the contaminated soil. Pea is vegetable crop plant, removes Cd from the contaminated soil. Brassica napus L. oilseed crop, removes Cd, Cu, Zn, Pb from the contaminated soil. Cajanus cajan L. is pulse crop, removes As, Cd from the contaminated soil. Cucumis sativus L. is vegetable crop, removes Pb from the contaminated water. Annual grass and broad leaf weed removes atrazine, simazine, propazine, prometryn from the soil. Chenopodium album L., Hordeum juhatum L., Panicum capillare L., Nepeta cataria L., Caedus nutans L., Poa annua an Fostuca sp. removes atrazine, phosphorus, nitrate, ammonium. The phytoremediation plant recovers natural resources and restricts outbreak of diseases in the nature. Further, the scientific studies and research may build products of phytoremediation controlled plants for removing heavy metal and agrochemicals residue from the soil.

Genetic Studies for Detection of Most Diverse and High Yielding Genotypes among Chickpea (Cicer arientinum L.) Germplasm

Genetic Studies for Detection of Most Diverse and High Yielding Genotypes among Chickpea (Cicer arientinum L.) Germplasm

Use of in-situ field chambers to quantify the influence of heat stress in chickpea (Cicer arientinum)

Chickpeas (Cicer arientinum) are commonly grown in parts of the world limited by heat stress, and this is likely to increase under climate change. In-field heat chambers were used to understand the response of chickpea pollen and yield to stress in-situ, and their utility in screening breeding material for heat tolerance. Replicated plots of a putative heat tolerant and heat susceptible cultivars were sown under commercial agronomic conditions in two seasons. Clear perspex air-conditioned chambers set at 4 °C above ambient temperature were placed over yield plots for 2 weeks at flowering. Chambers set to mimic ambient conditions, open-sided perspex shelters, and un-chambered plots of both cultivars were also randomised throughout the experiment to validate the method. Heat chamber treatment reduced yield between 9 % and 41 % compared to ambient chambers. The ambient conditions during the 2-week trial in both seasons were in the non-stressful range for heat, however both chilling stress and frost were observed. The heated chambers reached temperatures above 35 °C, but not for enough time to cause significant pollen sterility in either season. This was suggested by > 78 % pollen viability found at the conclusion of the heat chamber period. However, a significant chamber effect was observed, with plants under ambient chambers significantly taller than non-chamber and shelter plots. A yield reduction of between 11 % and 24 % was observed in the open-sided perspex shelter compared to the non-chamber treatment. The method will be refined by including set maximum daytime and minimum night temperatures to guarantee the targeted stress during the experimental period. The length of time of chamber application necessary to discriminate heat treatments, but minimise the chamber effects, will also be investigated. Despite this, heat-induced yield differences between genotypes suggests these chambers are a useful tool to screen for heat tolerance and research plant physiological responses.

Effect of Different Watering Regimes in Summer Season on Water Intake, Feed Intake, and Milk Production of Marecha She-camel (Camelus dromedarius).

Simple SummaryCamel livestock is a significant sector of agriculture in Pakistan. Punjab Marecha is the camel breed more diffuse for their production characteristics. In fact, this camel breed is well adapted to the desert ecosystem, tolerating elevated temperatures and dehydration. This study aimed to evaluate the effect of water deprivation on milk production performance in Marecha camels during the summer. Twelve she-camels in the early lactation stage were included. After seven days, camels were divided into three groups: Group 1 (G1) having access to water once a day, Group 2 (G2) having access once every 4 days, and Group 3 (G3) having access once every 6 days. Milk production and daily dry matter were decreased in camels deprived of water for 6 days. The results of the current study suggest that the camel is a productive animal even when subjected to water deprivation; however, its feed intake and milk production are decreased.Twelve lactating healthy Marecha she-camels in the early lactation stage during the summer at Camel Breeding and Research Station Rakh-Mahni (Pakistan) were included. All animals were fed with Medicago sativa and Cicer arientinum ad libitum and divided into three groups in relation to the access to water, after a period of seven days of adaptation to experimental conditions. Group 1 (G1) was considered as control having access to water once every day; Group 2 (G2) had access once every 4 days, while Group 3 (G3) had access once every 6 days. The duration of the study was 60 days with an adaptation period to experimental conditions of 7 days before the onset of the study. Dry matter intake (DMI) was calculated on a dry matter basis. On average the ambient temperature and relative humidity during the trial were 39–41 °C and 55–63%, respectively. The DMI, water intake, milk production, and body weight changes were affected (p < 0.001) during various watering regimes. The mean values of water intake were found to be 82.94 ± 1.34 L higher in G3 than G1 and G2.

Impact of various watering regimes on physiological and hematological parameters in intensively kept Marecha (Camelus dromedarius) she-camels in summer season.

Background:Camel is vital to daily life as a source of food and means of transportation, and just as importantly, its milk and urine have been used as medicine for diverse ailments since ancient times for people living in the desert region.Aim:The current study was planned to investigate the effect of different watering regimes on physiological and hematological parameters of lactating Marecha camels during the summer season. Methods:For this purpose, 12 lactating she-camels of almost similar age (8–13 years) and parity (2–5) were selected in a completely randomized design with four animals per treatment at the Camel Breeding and Research Station Rakh Mahni District Bhakkar. The trial was completed in 60 days, while 7 days were given as an acclimatization period for adaptation. The treatments were as follows: Group-1 (G-1) considered as the control had access to water once in a day; Group-2 (G-2) had access to water once in 4 days, and Group-3 (G-3) had access to water once in 6 days. Animals were housed individually for feeding and watering in an intensive management system. The same feed consisting of green fodder (Lucerne) and gram straw (Cicer arientinum) was offered to all animals. The average ambient temperature and relative humidity were 39°C–41°C and 55%–63%, respectively. Result:The mean values (p < 0.001) of water intake were found to be 82.94 ± 1.34 l in G-3, which was higher than G-1 and G-2. Blood analysis showed that packed cell volume, white blood cells, and hemoglobin were affected significantly (p < 0.001, p < 0.01, and p < 0.05, respectively). Conclusion:No serious changes in complete blood count and physiological parameters were reported in this study. So, this shows that the camel is unique in its physiological adaptation in hot and hostile environments.

APPLICATION OF MORINGA OLEIFERA LAM. SEEDS IN WASTEWATER TREATMENT

Coagulants play a vital role in treatment of raw water for both human and animal consumption. Aluminium sulphate is the most common and effective chemical coagulant for water treatment. However, chemical coagulants are costineffective, toxic, are not eco-friendly and may also cause severe health issues like cancers and neurologic disorders including Alzheimer’s disease. Therefore, natural and greener methods of water purification are crucial for safe and effective water treatment. Therefore, alternative natural and greener method of water purification is required. Seeds of Vicia faba, Cicer arientinum, Jatropha curcas, Vigna unguiculata, Zea mays, Vigna mungo etc. have also been used in water purification regimes. Moringa oleifera seeds contain anti-microbial properties and cationic water-soluble proteins (polyelectrolytes) which possess active coagulative properties that can remove the turbidity and heavy metals like Cu, Pb, Cr, Zn, etc from raw water, thus can treat impure water efficiently. Therefore, natural coagulants are preferred over chemical coagulants as they are feasible to use, affordable, eco-friendly and less toxic. This review provides explicit information on efficiency and use of M. oleifera seeds in water treatment and their relative comparison with that of routine chemical coagulants. Thus, further collaborative research (industry and academia) on the alternative greener techniques of water purification are still required to gain sustainability in water purification regimes in developing countries.