In the current study, the diesel engine performance, emission, and combustion have been investigated using tyre pyrolysis oil (TPO) and biodiesel blended with nano-additives. The effect of the blending ratio on fuel combustion and emission was evaluated. The tyre pyrolysis oil was derived from scrap tyres through the pyrolysis process and biodiesel was synthesized from used cooking oil (UCO) through the transesterification process. Moringa oleifera-derived strontium oxide (SrO) nanoparticles were mixed into the fuel to provide extra oxygen for better combustion. Three blended fuels were formulated as: a) 5 % biodiesel and 95 % TPO containing 50 ppm SrO nanoparticles (B5TPO95SrO50), b) 10 % biodiesel and 90 % TPO containing 100 ppm SrO nanoparticles (B10TPO90SrO100), c) 50 % TPO and 50 % biodiesel without nano-additives (B50TPO50). Among the blended fuels, B10TPO90SrO100 showed the best brake thermal efficiency at 31.4 % and a brake-specific fuel consumption of 0.21 kg/kWh at full load. The B5TPO95SrO50 blended fuel showed reduced emission parameters such as unburned hydrocarbon (HC), carbon monoxide (CO), and oxides of nitrogen (NOx) by 2.05 %, 8.30 %, and 18.00 %, respectively, as compared to the conventional diesel engine at an optimum engine load (27.9 Nm). Hence, waste tyre oil and UCO biodiesel blended with biogenic SrO nano additive can be considered a promising fuel for a sustainable environment.