Objective To establish a method for simultaneous quantitative determination of eight active components in Yiqing Soft capsule.Methods The separation was performed on Xtimate-C 18 chromatographic column with mobile phase consisted of water (containing 0.2% phosphoric acid)-acetonitrile (gradient elution) at a flow rate of 0.6 ml/min.Detecting wavelength was set at 280 nm and the column temperature was set at 30℃.Results The calibration curve of berberine,coptisine-hydrochloride,baicalein,aloe-emodin,rhein,emodin,chrysophanol and physcion showed good linear regression in test ranges (r > 0.9995); and the overall recoveries were in the range of 97%-103% (n=3).The overall RSD of precision test were less than 2%.Conclusion The extracting method is convenient and credible,and the quantitative method is accurate and repeatable,and it can be used in simultaneous quantitative determination of eight active components in Yiqing soft capsule. Key words: Yiqing soft capsule; HPLC; Content determination