Abstract
Objective To establish a method for simultaneous quantitative determination of eight active components in Yiqing Soft capsule.Methods The separation was performed on Xtimate-C 18 chromatographic column with mobile phase consisted of water (containing 0.2％ phosphoric acid)-acetonitrile (gradient elution) at a flow rate of 0.6 ml/min.Detecting wavelength was set at 280 nm and the column temperature was set at 30℃.Results The calibration curve of berberine,coptisine-hydrochloride,baicalein,aloe-emodin,rhein,emodin,chrysophanol and physcion showed good linear regression in test ranges (r ＞ 0.9995); and the overall recoveries were in the range of 97％-103％ (n=3).The overall RSD of precision test were less than 2％.Conclusion The extracting method is convenient and credible,and the quantitative method is accurate and repeatable,and it can be used in simultaneous quantitative determination of eight active components in Yiqing soft capsule. Key words: Yiqing soft capsule; HPLC; Content determination
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
