Chryseobacterium Indologenes Research Articles

Whole genome sequencing for deciphering the resistome of Chryseobacterium indologenes, an emerging multidrug-resistant bacterium isolated from a cystic fibrosis patient in Marseille, France.

We decipher the resistome of Chryseobacterium indologenes MARS15, an emerging multidrug-resistant clinical strain, using the whole genome sequencing strategy. The bacterium was isolated from the sputum of a hospitalized patient with cystic fibrosis in the Timone Hospital in Marseille, France. Genome sequencing was done with Illumina MiSeq using a paired-end strategy. The in silico analysis was done by RAST, the resistome by the ARG-ANNOT database and detection of polyketide synthase (PKS) by ANTISMAH. The genome size of C. indologenes MARS15 is 4 972 580 bp with 36.4% GC content. This multidrug-resistant bacterium was resistant to all β-lactams, including imipenem, and also to colistin. The resistome of C. indologenes MARS15 includes Ambler class A and B β-lactams encoding blaCIA and blaIND-2 genes and MBL (metallo-β-lactamase) genes, the CAT (chloramphenicol acetyltransferase) gene and the multidrug efflux pump AcrB. Specific features include the presence of an urease operon, an intact prophage and a carotenoid biosynthesis pathway. Interestingly, we report for the first time in C. indologenes a PKS cluster that might be responsible for secondary metabolite biosynthesis, similar to erythromycin. The whole genome sequence analysis provides insight into the resistome and the discovery of new details, such as the PKS cluster.

Biodegradation of flubendiamide by a newly isolated Chryseobacterium sp. strain SSJ1.

Flubendiamide, as a new class (Phthalic acid diamide) of pesticide with a wide spectrum of activity against lepidopteran pests extensively used alone or in combination with other insecticides in agriculture system to get protection from insect pests. Due to high specificity and limited approach towards non-target organism, the extensive use of this pesticide as an alternate for organophosphate and organochlorine pesticides, causing an eventual increase in environmental pollution. Five flubendiamide-resistant bacterial strains were isolated during the present study from agriculture soil considering previous history of pesticide application. Minimal inhibitory concentration of all the isolates showed strain SSJ1 was most efficient flubendiamide resistant organism. Biochemical tests and molecular sequencing of 16s rRNA was carried out which confirmed the isolate as Chryseobacterium indologenes strain SSJ1. UV–visible spectrophotometer study revealed that 89.06 % initial pesticide was removed by the isolate at optimum temperature of 35 °C and pH 7.0 with 5 days incubation period and is further confirmed by high-performance liquid chromatography (HPLC) analysis. Results of the present study however, suggest strain SSJ1 is most resistant to flubendiamide and can possibly be applied in the bioremediation of flubendiamide contaminated soils.

Bacterial and parasitic factors diagnosed in eel farmed in Poland in the years 2010-2014

European eel Anguilla anguilla L. is a fish species highly valued in European fisheries, currently reared under controlled conditions (Aquatic Recirculation System - RAS). In order to protect the health of fish, regular check-ups are carried out in specialized veterinary laboratories. Health hazards are recognized on the basis of clinical, pathological, microbiological, and parasitological surveys. The aim of the study was to analyze the results of health assessment of eels reared under controlled conditions, which was performed in the Diagnostics Laboratory of Fish and Crayfish Diseases, Department of Veterinary Hygiene, Voivodeship Veterinary Inspectorate in Olsztyn, in the period from 2010 to 2014. The results are presented according to the etiologic agents, divided into bacterial and parasitic diseases. In this period, 73 samples (100%) were examined, including 5 samples (6.85%) from glass eels and 68 (93.15%) from elvers. Microorganisms isolated from the collected material were mainly conditionally pathogenic, such as Pseudomonas fluorescens, Aeromonas hydrophila, Shewanella putrefaciens, Aeromonas sobria, Flavobacterium spp., and Chryseobacterium indologenes. The parasitological examination revealed infection with Trichodina spp., Ichthyophtirius multiphilliis, and Pseudodactylogyrus spp. The most frequently diagnosed were gill monogeneans, detected in 34 cases (75.55% of all positive parasitological test results). In 21 cases (61.8%) the infestation manifested itself as a disease. The remaining 13 cases were asymptomatic carriers (38.2%). The analysis shows that the main problem in the controlled rearing of eel are parasitic infestations, which may be endemic and pose a constant threat. Systematic monitoring and preventive measures are necessary throughout the rearing and fattening of fry to maintain a good health status of eels.

Draft genome sequence of a multidrug-resistant Chryseobacterium indologenes isolate from Malaysia.

Chryseobacterium indologenes is an emerging pathogen which poses a threat in clinical healthcare setting due to its multidrug-resistant phenotype and its common association with nosocomial infections. Here, we report the draft genome of a multidrug-resistant C. indologenes CI_885 isolated in 2014 from Malaysia. The 908,704-kb genome harbors a repertoire of putative antibiotic resistance determinants which may elucidate the molecular basis and underlying mechanisms of its resistant to various classes of antibiotics. The genome sequence has been deposited in DDBJ/EMBL/GenBank under the accession number LJOD00000000.

Prevalence of Resistant Gram-Negative Bacilli in Bloodstream Infection in Febrile Neutropenia Patients Undergoing Hematopoietic Stem Cell Transplantation: A Single Center Retrospective Cohort Study.

Bloodstream infection (BSI) is an important cause of morbidity and mortality in patients undergoing hematopoietic stem cell transplantation (HSCT). To evaluate the causative bacteria and identify risk factors for BSI associated mortality in febrile neutropenia patients undergoing HSCT, we collected the clinical and microbiological data from patients underwent HSCT between 2008 and 2014 and performed a retrospective analysis. Throughout the study period, among 348 episodes of neutropenic fever in patients underwent HSCT, 89 episodes in 85 patients had microbiological defined BSI with a total of 108 isolates. Gram-negative bacteria (GNB) were the most common isolates (76, 70.3%) followed by gram-positive bacteria (GPB, 29, 26.9%) and fungus (3, 2.8%). As to the drug resistance, 26 multiple drug resistance (MDR) isolates were identified. Resistant isolates (n = 23) were more common documented in GNB, mostly Escherichia coli (9/36, 25%) and Klebsiella pneumonia (6/24, 25%). A total of 12 isolated were resistant to carbapenem including 4 K pneumoniae (4/24, 16.7%), 3 Stenotrophomonas maltophilia, and 1 Pseudomonas aeruginosa and other 4 GNB isolates (Citrobacter freumdii, Pseudomonas stutzeri, Acinetobacter baumanii, and Chryseobacterium indologenes). As to the GPB, only 3 resistant isolates were documented including 2 methicillin-resistant isolates (Staphylococcus hominis and Arcanobacterium hemolysis) and 1 vancomycin-resistant Enterococcus faecium. Among these 85 patients with documented BSI, 11 patients died of BSI as primary or associated cause with a BSI-related mortality of 13.1 ± 3.7% and 90-day overall survival after transplantation at 80.0 ± 4.3%. Patients with high-risk disease undergoing allo-HSCT, prolonged neutropenia (≥15 days) and infection with carbapenem-resistant GNB were associated with BSI associated mortality in univariate and multivariate analyses. Our report revealed a prevalence of GNB in BSI of neutropenic patients undergoing HSCT. Patients with high-risk diseases with prolonged neutropenia and carbapenem-resistant GNB were independent risk factors for BSI-related mortality.

Optimization of operating parameters for anti-corrosive biopolymer production by Chryseobacterium Indologenes MUT.2 using central composite design methodology

This study describes the optimized conditions for using the mesophile bacterial strain Chryseobacterium Indologenes MUT.2 to obtain the maximum amount of biomass and biopolymer. The operating conditions in this study were pH = 5–9, temperature = 26–45 °C, and agitation = 70–210 rpm in a shaker flask. Furthermore, response surface methodology was applied to navigate the experimental data in accordance with central composite design. The optimal operating conditions were found to be pH = 7.7, temperature = 33 °C and agitation = 170 rpm. Under these conditions, the maximum biopolymer and biomass concentrations were obtained, i.e. 14.50 g/L and 2.00 g/L, respectively. Statistical results showed an adjusted-R2 of 0.9839 and 0.9313 for biopolymer and biomass concentrations, respectively. Moreover, analysis of variance demonstrated that pH–pH and temperature–temperature effects were found to be the most influential factors for biomass and biopolymer concentrations.

Changes in bacterial populations in refrigerated raw milk collected from a semi-arid area of Algeria

Most of the studies on milk microbiota have been performed on cows’ milk from animals reared in temperate and humid areas. In this work, changes in the bacterial consortium of refrigerated raw milk collected from cows grazed in a semi-arid area of Algeria were studied during 21 days of refrigerated storage. Twenty bacterial morpho-physiotypes were selected among 150 isolates from milk at different times over storage and identified by partial 16S rRNA gene sequencing. The dominant bacterial populations were characterized by a few species. Stenotrophomonas rhizophila, S. maltophilia and Chryseobacterium indologenes were predominant during the first 7 days, Lactobacillus pentosus and L. plantarum were isolated only after the 10th day, while Acinetobacter spp. was isolated at the end of storage. Compared to the current literature on milk from temperate zones, sluggish and incomplete microbial growth was observed with a long incubation phase ranging from 6.7 to 10.5 days and a maximum growth not exceeding 5.3 log colony-forming units (CFU) · mL−1. The composition of milk microbiota and its evolution over refrigeration suggest a bio-geographical characterization due to environmental factors. In particular, the possible presence of antimicrobial molecules coming from plants grazed in the semi-arid zone around the farm may account for the presence of selected microbial species and the extended milk shelf-life. Despite this being a preliminary work, these results encourage the use of arid herbs in animal feed and motivate scientists to focus their efforts on the study of biochemical composition of plants from arid areas and their antimicrobial activity.

Diversity, antimicrobial and antioxidant activities of culturable bacterial endophyte communities in Aloe vera.

Twenty-nine culturable bacterial endophytes were isolated from surface-sterilized tissues (root, stem and leaf) of Aloe vera and molecularly characterized to 13 genera: Pseudomonas, Bacillus, Enterobacter, Pantoea, Chryseobacterium, Sphingobacterium, Aeromonas, Providencia, Cedecea, Klebsiella, Cronobacter, Macrococcus and Shigella. The dominant genera include Bacillus (20.7%), Pseudomonas (20.7%) and Enterobacter (13.8%). The crude and ethyl acetate fractions of the metabolites of six isolates, species of Pseudomonas, Bacillus, Chryseobacterium and Shigella, have broad spectral antimicrobial activities against pathogenic Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Salmonella Typhimurium, Proteus vulgaris, Klebsiella pneumoniae, Escherichia coli, Streptococcus pyogenes and Candida albicans, with inhibition zones ranging from 6.0 ± 0.57 to 16.6 ± 0.57 mm. In addition, 80% of the bacterial endophytes produced 1,1-diphenyl-2-picrylhydrazyl (DPPH) with scavenging properties of over 75% when their crude metabolites were compared with ascorbic acid (92%). In conclusion, this study revealed for the first time the endophytic bacteria communities from A. vera (Pseudomonas hibiscicola, Macrococcus caseolyticus, Enterobacter ludwigii, Bacillus anthracis) that produce bioactive compounds with high DPPH scavenging properties (75-88%) and (Bacillus tequilensis, Pseudomonas entomophila, Chryseobacterium indologenes, Bacillus aerophilus) that produce bioactive compounds with antimicrobial activities against bacterial pathogens. Hence, we suggest further investigation and characterization of their bioactive compounds.

Plant-based culture media: Efficiently support culturing rhizobacteria and correctly mirror their in-situ diversity

Our previous publications and the data presented here provide evidences on the ability of plant-based culture media to optimize the cultivability of rhizobacteria and to support their recovery from plant-soil environments. Compared to the tested chemically-synthetic culture media (e.g. nutrient agar and N-deficient combined-carbon sources media), slurry homogenates, crude saps, juices and powders of cactus (Opuntia ficus-indica) and succulent plants (Aloe vera and Aloe arborescens) were rich enough to support growth of rhizobacteria. Representative isolates of Enterobacter spp., Klebsiella spp., Bacillus spp. and Azospirillum spp. exhibited good growth on agar plates of such plant-based culture media. Cell growth and biomass production in liquid batch cultures were comparable to those reported with the synthetic culture media. In addition, the tested plant-based culture media efficiently recovered populations of rhizobacteria associated to plant roots. Culturable populations of >106–108cfug−1 were recovered from the ecto- and endo-rhizospheres of tested host plants. More than 100 endophytic culture-dependent isolates were secured and subjected to morphophysiological identification. Factor and cluster analyses indicated the unique community structure, on species, genera, class and phyla levels, of the culturable population recovered with plant-based culture media, being distinct from that obtained with the chemically-synthetic culture media. Proteobacteria were the dominant (78.8%) on plant-based agar culture medium compared to only 31% on nutrient agar, while Firmicutes prevailed on nutrient agar (69%) compared to the plant-based agar culture media (18.2%). Bacteroidetes, represented by Chryseobacterium indologenes, was only reported (3%) among the culturable rhizobacteria community of the plant-based agar culture medium.

Chryseobacterium sediminis sp. nov., isolated from a river sediment.

A slightly yellow-pigmented strain (IMT-174T) isolated from a river sediment in Guyana was studied in detail for its taxonomic position. Cells of the isolate appeared rod-shaped and stained Gram-negative. Comparative 16S rRNA gene sequence analysis showed that the isolate had the highest sequence similarities to type strains of Chryseobacterium rhizoplanae (99.0 %), Chryseobacterium viscerum (98.9 %), Chryseobacterium lactis (98.8 %) and Chryseobacterium indologenes (98.6 %). The 16S rRNA gene sequence similarities to all other species of the genus Chryseobacterium were below 98.5 %. Fatty acid analysis of whole-cell hydrolysates of the strain resulted in a pattern typical of members of the genus Chryseobacterium, with fatty acids iso-C15 : 0, iso-C15 : 0 2-OH, iso-C17 : 1ω9c and iso-C17 : 0 3-OH as major compounds. The polyamine pattern contained predominantly sym-homospermidine. The major quinone was menaquinone MK-6 and the only lipid identified in the polar lipid profile was phosphatidylethanolamine. In addition, 13 unidentified lipids were detected in moderate to major amounts. DNA-DNA hybridizations with type strains of C. rhizoplanae, C. viscerum, C. lactis and C. indologenes resulted in values below 70 %. In addition to the genotypic differences, differentiating biochemical and chemotaxonomic properties confirmed that isolate IMT-174T represents a novel species, for which the name Chryseobacterium sediminis sp. nov. (type strain IMT-174T = LMG 28695T = CIP 110895T) is proposed.

Chryseobacterium indologenes: Distinguishing pathogen from contaminant in a neonate

Chryseobacterium indologenes (C. indologenes) is a rarely described clinical isolate, especially in pediatric patients. We report the second case of C. indologenes isolated from a neonatal endotracheal tube in an infant with Escherichia coli sep- ticemia. The challenge of distinguishing colonization, contamination and infection for this microorganism is discussed. Addi- tionally, the resistance of C. indologenes to antibiotics used in pediatric medicine is raised as a significant challenge for thera- peutic measures. We review nine cases of pediatric C. indologenes infection published in the English medical literature. C. indologenes is likely of low pathogenicity in the pediatric population.

Chryseobacterium indologenes carrying bla IND-1 isolated from blood obtained from a patient with adenocarcinoma

Chryseobacterium indologenes carrying bla IND-1 isolated from blood obtained from a patient with adenocarcinoma

Biodegradation of α and β-keratins by Gram-negative bacteria

The increasing amount of recalcitrant keratinous wastes generated as byproducts of agroindustrial processing has attracted attention to keratinolytic enzymes able to convert these proteins into valuable products through non-polluting processes. This work aimed to evaluate the biodegradation of α and β-keratins by three Gram-negative bacteria. All bacteria degraded feathers and feather meal and the production of keratinolytic proteases was confirmed through the azokeratin assay. An increase of thiol concentration was observed in the keratinous media cultivated with these strains, probably due to the reduction of disulfide bonds of the keratin substrate. In addition, the strains Chryseobacterium indologenes A22 and Aeromonas hydrophila K12 could also degrade hair and wool, showing a broad spectrum of keratinous wastes degradation. The influence of pH and feather meal concentration on C. indologenes A22 enzyme production was also investigated. This strain presented an optimal keratinase production on neutral pH and 15 g L−1 of substrate concentration.

Outbreak of Chryseobacterium indologenes infection related to a contaminated water pipe

Outbreak of Chryseobacterium indologenes infection related to a contaminated water pipe

An outbreak of chryseobacterium indologenes bacteremia: Pseudobacteremia of unrecognized sources

An outbreak of chryseobacterium indologenes bacteremia: Pseudobacteremia of unrecognized sources

Comment on "A Case of Healthcare Associated Pneumonia Caused by Chryseobacterium indologenes in an Immunocompetent Patient".

I am writing regarding the publication of S. A. Nemil et al., “A Case of Healthcare Associated Pneumonia Caused by Chryseobacterium indologenes in an Immunocompetent Patient” [1]. In the published case report, the authors state that the patient acquired a Healthcare Associated Pneumonia (HAP) caused by Chryseobacterium indologenes. Notwithstanding that the patient had a HAP, the evidence that the infection was caused by C. indologenes is insufficient and could possibly be questionable. (a) According to the authors both E. coli and C. indologenes were isolated from a transtracheal aspirate (TTA) sample; however it is suggested that the infection was caused only by C. indologenes. (b) The transtracheal aspirate (TTA) samples are ideal for bacteriology investigation; however an organism growth on a single TTA sample is not proof of the pathogen that causes the infection. The interpretation of the relevance of bacterial growth depends on the pathogen and it is high if the isolate is a typical pathogenic organism that causes respiratory tract infection/chest infection and significantly low if the isolated organism is an uncommon bacterium (Chryseobacterium indologenes). Chryseobacterium indologenes is an opportunistic pathogen, not normally present in the human microflora, although it is widely distributed in nature. The patient was not immunocompromised and therefore is more susceptible to an organism with low pathogenicity. Important information regarding the microbiology culture growth (quantitative or semiquantitative) and presence of inflammation were not reported in the document in order to estimate the significance of the culture. The presence of two organisms on the same culture reduces further the clinical significance of the culture result. In addition the diagnostic utility of TTA is likely decreased in a patient with a chronic airway disease, such as bronchiectasis, in which case a false-positive culture result should be expected due to common colonization below the level of the larynx [2]. (c) According to the authors the antimicrobial susceptibility test revealed that the “C. indologenes was resistant to carbapenems and sensitive to levofloxacin. Levofloxacin was added to antimicrobial therapy on the 6th day of admission. On follow-up, after the second day of therapy modification, improvement on blood gas parameters was observed and the patient was extubated.” The authors speculate that the patient had an infection caused by C. indologenes due to the patient not responding to the previous antibiotic treatment. The patient's antimicrobial therapy was modified to ertapenem on the 3rd day of admission (when urine culture yielded ESBL and after 3 days treatment with piperacillin-tazobactam), possibly indicating that the patient was possibly close to or less than 72 hours on ertapenem treatment when levofloxacin was added. It would be interesting to know if the 82-year-old patient had hypoalbuminaemia. Hypoalbuminaemia is rarely considered in clinical decisions but ertapenem is highly albumin bound (85–95%) and data from multiple studies showed that hypoalbuminaemia may have a profound effect on pharmacokinetics (failure to attain pharmacodynamic targets) and therefore could possibly lead to treatment failure or a delayed clinical response [3, 4]. In addition a large proportion of C. indologenes isolates are susceptible to piperacillin-tazobactam (the patient was initially treated with piperacillin-tazobactam) [5, 6]; however the C. indologenes susceptibility to piperacillin-tazobactam is not documented. On conclusion, the interpretation of the relevance of bacterial growth of uncommon bacterium with low pathogenicity (C. indologenes) in transtracheal aspirates needs to be carefully interpreted, including clinical cases when there is a clear clinical improvement after targeted antimicrobial therapy to the uncommon bacterium has been applied.

Identification and pathogenicity of bacteria in the Mediterranean corn borerSesamia nonagrioides Lefebvre (Lepidoptera: Noctuidae)

Sesamia nonagrioides (Lep.: Noctuidae) is one of the most serious pests of corn in Turkey and other Mediterranean countries. Although various cultural, chemical, and biological methods are used to control this pest, its damage still continues in all Mediterranean countries. In this study, to find an effective bacterium that can be used as a biocontrol agent against S. nonagrioides, we isolated 15 bacteria from S. nonagrioides larvae and evaluated the larvicidal potency of all isolates on the pest. According to their morphological, physiological, biochemical, and molecular properties, the isolates were identified as Achromobacter insolitus (Sn1), Morganella morganii (Sn2), Klebsiella pneumoniae (Sn3), Citrobacter freundii (Sn4), Arthrobacter protophormiae (Sn5), Chryseobacterium indologenes (Sn6), Bacillus thuringiensis (Sn7), Bacillus safensis (Sn8), Bacillus thuringiensis (Sn9), Bacillus thuringiensis (Sn10), Klebsiella pneumoniae (Sn11), Staphylococcus sciuri (Sn12), Enterobacter kobei (Sn13), Serratia marcescens (Sn14), and Microbacterium arborescens (Sn15). The results of the larvicidal activities of these isolates indicated that the mortality value obtained from all treatments varied from 25% to 93%, reaching 93% with B. thuringiensis (Sn10) for the third-instar larvae within 10 days of the application of 1.89 × 109 cfu/mL bacterial concentration at 25 °C in laboratory conditions. However, the dose-response experiments showed that increasing the concentration of bacteria gradually increased larval mortality, which reached 100% with a 2-fold concentration of Sn10. The findings of this study indicate that this isolate appears to be a promising biocontrol agent for use against S. nonagrioides.

Isolation and identification of nine bacteria strains producing Protein- glutaminase

[Objective]Isolating and screening the strains producing Protein- glutaminase and then making Identification of them.[Methods]726 soil samples from all over the country were collected,from which the strains producing Protein- glutaminase were enriched with Cbz- Gln- Gly as the sole nitrogen source by preliminary screening in plates.Then the isolates were identified in morphology,physiology and molecular biology.The enzyme activity of deamidation was also been detected.[Results]Totally 9 strains that could produce PG were isolated,of which 7 bacteria were identified as Chryseobacterium indologenes,one was identified as Chryseobacterium proteolyticum and another was identified as Chryseobacterium.gleum.[Conclusions]The 9 strains were fermented in aerobic,and the PG activity in fermentation supernatant was tested. The results shown that Chryseobacterium indologenes ZYF120413- 7 had the highest enzyme activity with 0.7168 U / m L. Chryseobacterium. gleum came the lowest with only 0.1029 U / m L.This study enlarged the source of the PG producing strains,lying a good foundation for the following research.

Chryseobacterium rhizoplanae sp. nov., isolated from the rhizoplane environment.

A slightly yellow pigmented strain (JM-534(T)) isolated from the rhizoplane of a field-grown Zea mays plant was investigated using a polyphasic approach for its taxonomic allocation. Cells of the isolate were observed to be rod-shaped and to stain Gram-negative. Comparative 16S rRNA gene sequence analysis showed that the isolate had the highest sequence similarities to Chryseobacterium lactis (98.9 %), Chryseobacterium joostei and Chryseobacterium indologenes (both 98.7 %), and Chryseobacterium viscerum (98.6 %). Sequence similarities to all other Chryseobacterium species were 98.5 % or below. The fatty acid analysis of the strain resulted in a Chryseobacterium typical pattern consisting mainly of the fatty acids C15:0 iso, C15:0 iso 2-OH, C17:1 iso ω9c, and C17:0 iso 3-OH. DNA-DNA hybridizations with the type strains of C. lactis, C. joostei, C. viscerum and C. indologenes resulted in values below 70 %. Genomic fingerprinting showed that the isolate was very different to the type strains of these species. Differentiating biochemical and chemotaxonomic properties showed that the isolate JM-534(T) represents a novel species, for which the name Chryseobacterium rhizoplanae sp. nov. (type strain JM-534(T) = LMG 28481(T) = CCM 8544(T) = CIP 110828(T)) is proposed.

In vitro and in vivo antibacterial activity of Pogostone

Background Our pervious antibacterial studies on several traditional Chinese medicines have found that Patchouli oil from Pogostemon cablin had significant antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA), which has spread worldwide and infected innumerable people. In order to find the more active natural substances in Patchouli oil, one of the major components, Pogostone, was isolated and its antibacterial activity was evaluated in vitro and in vivo in this study. Methods In vitro test, Pogostone was screened for antimicrobial properties against 83 bacteria comprising 35 gram positive and 48 gram negative bacteria strains via the agar double dilution method. In vivo test, specific pathogen free (SPF) strain of both male and female white Kunming mice, weighing 18-22 g, were used to test the protective ability of Pogostone after being injected with the median lethal doses (MLDs) of the tested strains. Results In vitro test, Pogostone could inhibit both gram negative bacteria (0.098-1 600 μg/ml) and gram positive bacteria(0.098-800 μg/ml). For Corynebacterium xerosis and some Chryseobacterium indologenes, the minimum inhibitory concentration (MIC) values of Pogostone were extremely low (<0.098 μg/ml). It was significant that Pogostone was also active against some drug-resistant bacteria like MRSA. Furthermore, Pogostone showed antibacterial activity in vivo against Escherichia coli (E. coli) and MRSA via intraperitoneal injection. Ninety percent of the mice infected with E. coil could be protected at the concentrations of 50 and 100 mg/kg, and 60% of the mice at 25 mg/kg, while the rate of protection for the mice infected with MRSA was 60% and 50% at doses of 100 and 50 mg/kg, respectively. Conclusion Pogostone could be developed as a potential antibacterial agent for clinical therapy.