Isolation and identification of nine bacteria strains producing Protein- glutaminase

Abstract

[Objective]Isolating and screening the strains producing Protein- glutaminase and then making Identification of them.[Methods]726 soil samples from all over the country were collected,from which the strains producing Protein- glutaminase were enriched with Cbz- Gln- Gly as the sole nitrogen source by preliminary screening in plates.Then the isolates were identified in morphology,physiology and molecular biology.The enzyme activity of deamidation was also been detected.[Results]Totally 9 strains that could produce PG were isolated,of which 7 bacteria were identified as Chryseobacterium indologenes,one was identified as Chryseobacterium proteolyticum and another was identified as Chryseobacterium.gleum.[Conclusions]The 9 strains were fermented in aerobic,and the PG activity in fermentation supernatant was tested. The results shown that Chryseobacterium indologenes ZYF120413- 7 had the highest enzyme activity with 0.7168 U / m L. Chryseobacterium. gleum came the lowest with only 0.1029 U / m L.This study enlarged the source of the PG producing strains,lying a good foundation for the following research.