Chromatographic Fingerprint Research Articles

α-Glucosidase inhibitors screening from Cyclocarya paliurus based on spectrum-effect relationship and UPLC-MS/MS.

Cyclocarya paliurus is an edible and medicinal plant exhibiting significant hypoglycemic effect. However, its active components are still unclear and need further elucidation. In this research, the active components of the leaves of C. paliurus responsible for the α-glucosidase inhibitory activity were screened and identified based on a spectrum-effect relationship study in combination with ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) analysis. The 70% ethanol eluate fraction of the leaves of C. paliurus with the strongest α-glucosidase inhibitory activity was obtained after extraction and purification with macroporous resin. Their chromatographic fingerprints (15 batches) were established by UPLC analysis and 32 common peaks were specified by similarity analysis. Their IC50 values for α-glucosidase inhibition were measured by an enzymatic reaction. Several multivariate statistical analysis methods including hierarchical cluster analysis, principal component analysis, partial least square analysis and gray relational analysis were applied to explore the spectrum-effect relationship between common peaks and IC50 values, and the chromatographic peaks making a large contribution to efficacy were screened out. To further elucidate the active components of leaves of C. paliurus, the 70% ethanol eluate fraction was characterized by UPLC-MS/MS analysis, and 10 compounds were identified. This study provides a valuable reference for further research and development of hypoglycemic active components of C. paliurus.

Discrimination of Adulterated Ginkgo Biloba Products Based on 2T2D Correlation Spectroscopy in UV-Vis Range.

Ginkgo biloba is a popular medicinal plant widely used in numerous herbal products, including food supplements. Due to its popularity and growing economic value, G. biloba leaf extract has become the target of economically motivated adulterations. There are many reports about the poor quality of ginkgo products and their adulteration, mainly by adding flavonols, flavonol glycosides, or extracts from other plants. In this work, we developed an approach using two-trace two-dimensional correlation spectroscopy (2T2D COS) in UV-Vis range combined with multilinear principal component analysis (MPCA) to detect potential adulteration of twenty G. biloba food supplements. UV-Vis spectral data are obtained for 80% methanol and aqueous extracts in the range of 245–410 nm. Three series of two-dimensional correlation spectra were interpreted by visual inspection and using MPCA. The proposed relatively quick and straightforward approach successfully differentiated supplements adulterated with rutin or those lacking ginkgo leaf extract. Supporting information about adulteration was obtained from the difference between the DPPH radical scavenging capacity of both extracts and from chromatographic (HPLC-DAD) fingerprints of methanolic samples.

Comprehensive evaluation of Loblolly fruit by high performance liquid chromatography four wavelength fusion fingerprint combined with gas chromatography fingerprinting and antioxidant activity analysis

Loblolly fruit (LBF) is mainly used as raw material for beverage, but there are few researches on its quality evaluation or control. The aim of this study was to develop comprehensive evaluation methods for the quality control of Loblolly fruit. firstly, double wavelength coefficient ratio spectrum was used to identify the purity of chromatographic fingerprint peak. It is very important to identify the purity of fingerprint peaks because only the quantitative determination of pure chromatographic peaks is meaningful for its efficient quality control. Then, multi-wavelength fusion fingerprint was established to avoid one-sidedness of a single wavelength for further evaluation by systematically quantified fingerprint method (SQFM). According to the outcome of Pm, 25 batches of LBF were classified into two classifications by hierarchical cluster analysis, which was consistent with the SQFM evaluation results. Two active components, gallic acid (GAC) and ethyl gallate (EGA) in LBF, were quantitatively determined by quantitative analysis of multi-components by single marker (QAMS). In addition, the fingerprint efficacy relationship was established using an off-line antioxidant system and partial least-squares model to explore the connection between chemical components and antioxidant activities. Finally, the evaluation results of high-performance liquid chromatography and gas chromatography were integrated by the mean algorithm, which could reduce the error caused by single method. The results showed that the proposed strategy could provide a method for quality evaluation of LBF and even other traditional Chinese medicines (TCMs).

Chromatographic fingerprint application possibilities in food authentication

The aim of the study was to compare the effectiveness of the use of low-peak chromatographic fingerprints for the differentiation of various food products. Three groups of unprocessed products (mushrooms, hazelnuts and tomatoes), food preparations (bread, dried herbs and tomato juice) and alcoholic beverages (vodka and two types of blended whiskey) were examined. A commercial electronic nose based on ultrafast gas chromatography (acquisition time 90 s) with a flame ionization detector was used for the research. Static headspace was used as a green procedure to extract volatile compounds without modifying the food matrix. Individual extraction conditions were used for each product group. Similarities and differences between profiles were analyzed by simple Principal Components Analysis. The similarity rating was determined using the Euclidean distances. Global model was built for recognition chromatographic fingerprints of food samples. The best recognition results were 100% and 89% for tomato juices, spices, separate champignon elements and hazelnuts. On the other hand, the worst recognition results were 56% and 77% for breads and strong alcoholic beverages.

Shelf-life evaluation of Kasisadya Ghritam prepared by two different methods

BACKGROUND: Kasisadya ghritam (KG) is a herbomineral formulation comprising 32 natural ingredients mentioned in Sharangadhara samhita. Different methods of preparation have been mentioned regarding the same especially in respect to the heating pattern, that is, Agnipaki and Suryatapi. As this medicine has shown promising results in various ailments since the ages, the use of this medicine has become very prevalent in recent clinical practices. The globalization and commercialization of Ayurveda sought for the stability of this medicine is unfortunately not evaluated to date. Therefore, a plan had been made to evaluate the shelf life of this formulation by following parameters prevalent in the respective scenario. The aim of this study was to evaluate the shelf life of both the samples of Kasisadya ghritam Suryatapi (KGS) and Kasisadya ghritam Agnipaki (KGA) through basic analytical parameters and chromatographic fingerprinting. MATERIALS AND METHODS: Stability period of both the samples was determined by the accelerated stability study as per International Council of Harmonization (ICH) Guidelines Q1A (R2). The test drugs were stored at 40°C ± 2°C temperature and 75 ± 5% relative humidity and withdrawn at the intervals of 0, 1st, 3rd, and 6th months of storage. Physicochemical parameters of KGS and KGA were analyzed at the intervals of 0, 1, 3, and 6 months, whereas chromatographic fingerprinting was done initially and after 6 months of the study. Based upon the observations, intercept, slope, 10% degradation of the sample, and the shelf life of both the samples were calculated. RESULTS: Basic analytical parameters showed both the samples deteriorate by time. By calculating the analytical data, 10% degradation of KGS was found in specific gravity (0.83), viscosity (194.4), acid value (6.58), peroxide value (1.97), and total fatty matter (78.83). The data of KGA revealed 10% degradation in parameters, that is, specific gravity (0.8295), viscosity (496.8), acid value (0.864), peroxide value (1.341), and total fatty matter (82.81). Rf values obtained from high-performance thin-layer chromatography (HPTLC) of both samples initially and after 6 months showed minimum deterioration of the product. Microbial count and heavy metals were below the permissible limits in both the samples. Accelerated stability study reveals the shelf life of KGS as 3.8 years, whereas that of KGA as 4.4 years. CONCLUSION: Rule 161 B of Drugs and Cosmetics Rule, 1945 mentioned the shelf life of medicated Ghrita as 2 years. This time period is general, and different formulations may have different shelf lives based upon several factors. The samples tested in the current study, that is, KGS and KGA, are found to have a shelf life of 3.8 and 4.4 years, respectively, which are more than the time specified in the guidelines. The findings suggest that the sample prepared by subjecting to heat has more shelf life than the sample prepared by subjecting to sunlight.

Quality value transmitting of substance benchmarks of Zhuru Decoction

A total of 18 batches of Zhuru Decoction samples were prepared. Chromatographic fingerprints were established for Zhuru Decoction and single decoction pieces, the content of which was then determined. The extraction rate ranges, content, and transfer rate ranges of puerarin, liquiritin, and glycyrrhizic acid, together with the common peaks and the similarity range of the fingerprints, were determined to clarify key quality attributes of Zhuru Decoction. The 18 batches of Zhuru Decoction samples had 25 common peaks and the fingerprint similarity higher than 0.95. Puerariae Lobatae Radix, Glycyrrhizae Radix et Rhizoma, and Zingiberis Rhizoma Recens had 21, 3, and 1 characteristic peaks, respectively. The 18 batches of samples showed the extraction rates within the range of 18.45%-25.29%. Puerarin had the content of 2.20%-3.07% and the transfer rate of 38.5%-45.9%; liquiritin had the content of 0.24%-0.85% and the transfer rate of 15.9%-37.5%; glycyrrhizic acid had the content of 0.39%-1.87% and the transfer rate of 16.2%-32.8%. In this paper, the quality value transmitting of substance benchmarks of Zhuru Decoction was analyzed based on chromatographic fingerprints, extraction rate, and the content of index components. A scientific and stable method was preliminarily established, which provided a scientific basis for the quality control and formulation development of Zhuru Decoction.

High-performance liquid chromatography fingerprint of marine traditional Chinese medicine haliotidis

Objective: Haliotidis, the dried muscle of abalone, has long been used as a marine traditional Chinese medicine (TCM) due to its high medicinal and nutritive values, whereas few reports contributed to its quality control. This study aimed to establish the high-performance liquid chromatography (HPLC) fingerprints of Haliotidis. Materials and Methods: The HPLC fingerprints of Haliotidis, the manufacturing products of dried muscle of abalone, were analyzed by reverse-phase HPLC, using a YMC HPLC C18 column (250 mm × 4.6 mm, 5 μm). The gradient elution mode was applied with mobile phase starting from 5% acetonitrile and 0.1% phosphoric acid aqueous solution to 100% acetonitrile in 70 min at a flow rate of 1.0 mL/min. The detection wavelength was set at 190 nm. Results: Through compared the HPLC fingerprint chromatograms of 14 batches of Haliotidis, 25 peaks were assigned as the characteristic common peaks, of which one index component L-phenylalanine was identified by comparison with its standard substance. The established HPLC fingerprint analysis method of Haliotidis was validated with good precision, repeatability, and stability. Based on the above study, a reference HPLC fingerprint chromatogram of Haliotidis was proposed. Conclusions: It is the first time to establish the HPLC fingerprint chromatogram of TCM Haliotidis, which could contribute to the establishment of its quality control system.

Pharmacognostic study of Calotropis procera (Aiton) W.T. Aiton root and stem

Calotropis procera (Aiton) W.T. Aiton, belonging to the family Apocynaceae is an important medicinal shrub. It is widely used in folklore and Ayurvedic system of medicine. Various parts of this plant were used to treat human diseases such as Pama (Scabies), Vicharchika (Eczema), Kasa (Cough), Mukhakrishnatwa (Hyperpigmentation of the face), Pleehodara (Splenomegaly) and preparation of Ayurvedic as well as traditional medicines like Arkalavana and Arkataila. The present communication deals with a detailed account of the pharmacognostic study of C. procera root and stem. The study includes macroscopy, microscopy, powder microscopy studies, physicochemical, phytochemical analysis, heavy metal tests, screening of microbiological parameters and development of High-Performance Thin Layer Chromatography (HPTLC) fingerprints profile of C. procera root and stem. HPTLC fingerprints profile of ethanolic extract was done by using mobile phase toluene: ethyl acetate: formic acid for root and stem.

Phytochemical characterization, and antioxidant and antibacterial activities of the hydroethanolic extract of Anadenanthera peregrina stem bark.

The Brazilian Cerrado biome consists of a great variety of endemic species with several bioactive compounds, and Anadenanthera peregrina (L.) Speg is a promising species. In this study, we aimed to perform phytochemical characterization and evaluate the antioxidant and antibacterial activities against Staphylococcus aureus and Escherichia coli of the hydroethanolic extract of A. peregrina stem bark. The barks were collected in the Botanical Garden of Goiânia, Brazil. The hydroethanolic extract was obtained by percolation and subjected to physicochemical screening, total phenolic content estimation, high-performance liquid chromatography (HPLC) fingerprinting, and antioxidant (IC50 values were calculated for the 2,2-diphenyl-1-picrylhydrazyl assay - DPPH) and antibacterial activity determination. The pH of the extract was 5.21 and density was 0.956 g/cm3. The phytochemical screening indicated the presence of cardiac glycosides, organic acids, reducing sugars, hemolytic saponins, phenols, coumarins, condensed tannins, flavonoids, catechins, depsides, and depsidones derived from benzoquinones. The extract showed intense hemolytic activity. The total phenolic content was 6.40 g GAE 100 g-1. The HPLC fingerprinting analysis revealed the presence of gallic acid, catechin, and epicatechin. We confirmed the antioxidant activity of the extract. Furthermore, the extract did not inhibit the growth of E. coli colonies at any volume tested, but there were halos around S. aureus colonies at all three volumes tested. These results contribute to a better understanding of the chemical composition of A. peregrina stem bark and further support the medicinal applications of this species.

Identification of Cordyceps cicadae and Tolypocladium dujiaolongae based on ITS sequences and chemical pattern recognition method

Based on ITS sequences, the molecular identification of Cordyceps cicadae and Tolypocladium dujiaolongae was carried out, and high-performance liquid chromatography(HPLC) fingerprint combined with chemical pattern recognition method was established to differentiate C. cicadae from its adulterant T. dujiaolongae. The genomic DNA from 10 batches of C. cicadae and five batches of T. dujiaolongae was extracted, and ITS sequences were amplified by PCR and sequenced. The stable differential sites of these two species were compared and the phylogenetic tree was constructed via MEGA 7.0. HPLC was used to establish the fingerprints of C. cicadae and T. dujiaolongae, and similarity evaluation, cluster analysis(CA), principal component analysis(PCA), and partial least squares discriminant analysis(PLS-DA) were applied to investigate the chemical pattern recognition. The result showed that the sources of these two species were different, and there were 115 stable differential sites in ITS sequences of C. cicadae and T. dujiao-longae. The phylogenetic tree could distinguish them effectively. HPLC fingerprints of 18 batches of C. cicadae and 5 batches of T. dujiaolongae were established. The results of CA, PCA, and PLS-DA were consistent, which could distinguish them well, indicating that there were great differences in chemical components between C. cicadae and T. dujiaolongae. The results of PLS-DA showed that six components such as uridine, guanosine, adenosine, and N~6-(2-hydroxyethyl) adenosine were the main differential markers of the two species. ITS sequences and HPLC fingerprint combined with the chemical pattern recognition method can serve as the identification and differentiation methods for C. cicadae and T. dujiaolongae.

Assessment of pharmacognostic specification of Cannabis sativa leaves in Thailand.

ABSTRACTLack of quality control can affect the safety, efficacy, and acceptability of herbal products that may lead to health problems. Cannabis sativa L. (Cannabaceae) has been widely used as an ethnomedicinal practice for its medicinal values. This study aims to establish pharmacognostic specifications of C. sativa as per standard procedures. Macroscopic-microscopic characteristics, physicochemical parameters, thin-layer chromatography (TLC) fingerprinting, and phytochemical screening of C. sativa leaves collected from various locations throughout Thailand were investigated. Leaves are palmate consists of seven leaflets with green color, margin is serrate with acuminate apex. Anomocytic stomata were found in the upper epidermis while unicellular and glandular trichomes with cystolith were found in the lower epidermis and the epidermis layer covered with cuticle. The physicochemical analysis revealed that the loss on drying (4.068 ± 0.084 %w/w) was within acceptable limits, total ash (14.360 ± 0.084%w/w), acid insoluble ash (2.726 ± 0.080%w/w), ethanol-soluble extractive (11.101 ± 0.223%w/w), water-soluble extractive (23.038 ± 0.306%w/w), and water content (7.523 ± 0.524%w/w). TLC fingerprint showed nine spots with Rf value 0.14, 019, 0.23, 0.29, 0.32, 0.45, 0.58, 0.70, and 0.76. Phytochemical screening of Cannabis leaves indicated the presence of phenolic compounds, flavonoids, alkaloids, diterpenes, triterpenes, and steroids. This study provided referential data for the accurate plant identity, and establishment of cannabis leaves monograph in Thailand.

A COMPREHENSIVE ANALYTICAL APPROACH FOR QUALITY EVALUATION OF FLAVONOID-RICH EXTRACT OF Glycyrrhiza glabra (GutGard®)

Quality evaluation of botanical preparations is still evolving globally due to the complex, variable, and unknown phytochemical compositions of herbs. Accordingly, the quality of commercially available products needs to be better defined and controlled to ensure consistent health benefits and safety for consumers. This study aims to develop a comprehensive analytical methodology involving both phytochemical and biological evaluations towards achieving a meaningful quality control of commercial batches of a flavonoid-rich extract (GutGard®) derived from Glycyrrhiza glabra. Nine different commercial batches of the extract were analyzed to establish the chromatographic fingerprint of flavonoids as well as biological consistency using in-vitro assays for evaluating antioxidant and anti-inflammatory activity. A total of 53 peaks were assigned using MS/MS as the “common peaks” and nine peaks as “characteristic peaks” in the fingerprint of all the nine batch samples. Quantitative determination of the latter was achieved with a validated HPLC method. The finding revealed that all the examined samples were enriched with flavonoids, although with varied contents. The biological assays complemented the phytochemical analysis by way of providing a range of IC50 values that represent the overall chemistry of the extract including both the known and unknown constituents.

Chromatographic Fingerprinting of an Ayurvedic Formulation by High-Performance Thin Layer Chromatography Method

In the present study, an attempt has been made to develop a high-performance thin layer chromatography fingerprinting of gallic acid, piperine and curcumin from an Ayurvedic formulation, Dekofcyn tablet. Chromatographic separation was achieved on the plate pre-coated with silica gel 60F254 as the stationary phase. The solvent system consisted of toluene:ethyl acetate:formic acid:glacial acetic acid in the ratio (5:3.5:1:1 v/v/v/v) respectively. The retardation factor value was calculated at 0.35 (±0.02), 0.66 (±0.02) and 0.89 (±0.02) for gallic acid, piperine and curcumin respectively. Densitometric analysis was carried out in the isoabsorptive mode at 295 nm. The regression analysis was carried out in the range of 100-700 ng/spot for gallic acid and piperine, 400-1600 ng/spot for curcumin and the regression coefficient for all three markers was found to be 0.99 respectively. Validation of the method was performed according to international conference on hominization guidelines Q2 (R1). This method can also be used to evaluate other formulations containing these markers as one of the constituents, thus conforming to the need for ensuring the quality of an Ayurvedic formulation.

An Eco-Friendly Proposal by Integrating Chromatographic Fingerprinting and Multivariate Control Chart in a Non-Target Analysis to Evaluate Grape Juices from Different Farming Practices

A potential eco-friendly method without organic solvents is presented by integrating a chromatographic fingerprint and multivariate control chart based on Q residuals to differentiate grape juices from different farming practices. The sample preparation was only water dilution, and the mobile phase was water acidified with sulfuric acid, which can be readily neutralized before its disposal. The proposed method is shown to be a simple way to distinguish between organic and non-organic grape juices in a non-target way, successfully evaluating an external validation data set, where organic and non-organic samples were correctly assigned. Through the chromatographic profile, it is possible to suggest that one of the species responsible for this distinction may be from the anthocyanins class.

Multi-wavelength fusion column fingerprint technology combined with chemometric analysis to evaluate the overall quality of the Gardenia jasminoides root.

Chromatographic fingerprinting provides effective technical means for quality evaluation of traditional Chinese medicine. In this work, a novel multi-wavelength fusion column fingerprint was obtained by intelligent selection of chromatographic peaks from different wavelengths, which displayed the maximum peak area information under the optimal wavelength at the same retention time. Here, the Gardenia jasminoides root was selected as a sample. The multi-wavelength fusion column fingerprint graph of the Gardenia jasminoides root was constructed from five wavelengths (203 nm, 210 nm, 238 nm, 250 nm and 330 nm). The peak capacity, peak resolution, the number of common peaks and similarity were used to evaluate the performance. The 19 batches of Gardenia jasminoides root were classified into three categories with clear distinction between origin categories based on the multi-wavelength fusion column fingerprint combined with chemometrics, including hierarchical cluster analysis and principal component analysis. Nine markers of variation that led to differences between batches were screened by orthogonal partial least squares discriminant analysis. This study demonstrated that the classification model based on the multi-wavelength fusion column fingerprint was better than that on a single-wavelength, and the fusion fingerprint was suitable for the identification and quality control of traditional Chinese medicine with more comprehensive chemical composition information and more accurate prediction ability.

UPLC fingerprint and determination of five components of substance benchmark of classical prescription Shentong Zhuyu Decoction

Fingerprints of 18 batches of substance benchmark of Shentong Zhuyu Decoction(SZD) were established by UPLC under the following conditions: Waters Sun Fire C_(18) column(3.0 mm×150 mm, 3.5 μm), column temperature of 35 ℃, gradient elution with mobile phase of acetonitrile(A)-0.1% phosphoric acid aqueous solution(B) at the flow rate of 0.4 mL·min~(-1), and detection by wavelength switching. A total of 16 common peaks were identified. The similarities among the fingerprints were calculated by Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Edition) and the result showed they were in the range of 0.911-0.988. Based on the 16 common peaks, cluster analysis(CA), principal component analysis(PCA), and partial least square discriminant analysis(PLS-DA) all categorized the 18 batches of samples into two groups(S1, S2, S5-S8, S14, and S17 in one group, and S1, S2, S5-S8, S14, and S17 in another), and 11 most influential components were screened. Five known components with great difference among samples(hydroxysafflor yellow A, ferulic acid, benzoic acid, ecdysone, and ammonium glycyrrhizinate) were determined. The combination of multi-component content determination and fingerprints can reflect the overall cha-racteristics of the primary standards of SZD, which is simple, feasible, reproducible, and stable. This study can serve as a reference for the quality control of the primary standards of SZD.

Chemical Standards and HPTLC Finger Print Profiles of a Siddha Polyherbal Formulation - Kadukkai Legiyam

To study physico-chemical, phytochemical and high performance thin layer chromatography of a Siddha drug “Kadukkai Legiyam” (KL). The prepared Kadukkai Legiyam (KL) was prepared as per the standard operating procedures mentioned in literature. Then the drug was subjected to physicochemical parameters, phytochemical screening, thin layer chromatographic photo documentation (TLC), high performance thin layer chromatographic (HPTLC) finger print profile of hexane, chloroform, ethanol and hydro alcohol (1:1) extracts. Different extracts of the drug showed distinct TLC and HPTLC finger print patterns which will be unique to this drug. This study giving information about physiochemical and phytochemical analysis and HPTLC fingerprint profile of different extracts, the integration spectrum which will useful in standardizing the raw drugs and future comparison studies.

Chemical composition of essential oils from the leaves of Mosiera bullata (Britton & P.Wilson), an unexplored Cuban endemic species

ABSTRACT Mosiera Small is a genus of Myrtaceae family with about forty species identified, including the Cuban endemic plant Mosiera bullata (Britton & P.Wilson) Bisse subsp. bullata. Mosiera species have been little investigated, and this is the first study analysing the composition of Mosiera bullata. Fresh leaf samples of M. bullata were collected from different locations near Santa Clara city, Cuba, and their essential oil was extracted. Using GC/MS, nineteen components (89.68% of the oil) were identified. The major compounds were found to be 1,8-cineole and eugenol, and a GC/FID method was analytically validated for their quantification. Chromatograms (GC/FID) of the sixteen samples studied were proposed as a fingerprint of the volatile composition of M. bullata leaves. Chemometric methods assured the reliability of the chromatographic fingerprinting. Therefore, in the present work is suggested, for the first time, a reliable and consistent chemical profile of M. bullata essential oil.

Establishment of Holistic Quality Control Methods for Nelumbinis Folium Containing Alkaloids and Flavonoids with Simple HPLC Conditions.

In this study, a positive charged C18 column was used to explore its performance in analysis of herbal medicines containing alkaloids and flavonoids with Nelumbinis Folium (NF) as an example. A chromatographic fingerprint analysis method was established by high performance liquid chromatography-diode array detector with commonly used 0.1% formic acid as mobile phase additive and this method could simultaneously detect both alkaloids and flavonoids with good peak shape. It is noted that the HPLC conditions were directly applied in the HPLC-ESI-Orbitrap-MS/MS analysis, and 12 common peaks were identified. In the quantification method of nuciferine, compared with common C18 column, good performance was observed, including sharp and symmetric peak shape of nuciferine, and no obvious retention time shift in chromatogram. The fingerprint method and quantification method of nuciferine and quercetin-3-O-glucuronic acid could be readily utilized as quality control methods for NF and its related preparations.

Assessing the Quality of Calyx of Physalis alkekengi L. var. franchetii Based on Quantitative Analysis of Q-Marker Combined with Chemometrics and Machine Learning Algorithms

Physalis alkekengi L. var. franchetii (PALF) is a traditional Chinese medicine, which is well known for its antimicrobial, anti-inflammatory, antipyretic, and expectorant properties. Its fruits and fruiting calyxes are used as dietary supplements and traditional herbs in China. However, the quality of calyxes is uneven, and it is prone to getting moldy or moth-eaten during storage. High-performance liquid chromatography (HPLC) fingerprints and multivariate chemometric methods were combined to evaluate quality, and three representative compounds were chosen as the quality markers (Q-markers). Hierarchical cluster analysis (HCA) and principal component analysis (PCA) provided a clear discrimination of PALF samples. Through further verification by partial least squares discriminant analysis (PLS-DA), backpropagation artificial neural network (BP-ANN), machine learning, and combination with the determination of the content, biology, and pharmacology effect judgment, galuteolin, rutin, and physalin O could be used as Q-markers that their contents affect the quality of PALF grade evaluation. A simple method was established to rapidly assess the quality of PALF that is important for its clinical application and storage and provide a reference for evaluating the quality of materials used in Chinese medicine.