Chromatic Sensitivity Research Articles

The Chromatic and Achromatic Contrast Sensitivity Function in the Far Periphery

The Chromatic and Achromatic Contrast Sensitivity Function in the Far Periphery

Consequences of fixational eye movements for chromatic sensitivity

Consequences of fixational eye movements for chromatic sensitivity

ColorVideoVDP: A visual difference predictor for image, video and display distortions

ColorVideoVDP is a video and image quality metric that models spatial and temporal aspects of vision for both luminance and color. The metric is built on novel psychophysical models of chromatic spatiotemporal contrast sensitivity and cross-channel contrast masking. It accounts for the viewing conditions, geometric, and photometric characteristics of the display. It was trained to predict common video-streaming distortions (e.g., video compression, rescaling, and transmission errors) and also 8 new distortion types related to AR/VR displays (e.g., light source and waveguide non-uniformities). To address the latter application, we collected our novel XR-Display-Artifact-Video quality dataset (XR-DAVID), comprised of 336 distorted videos. Extensive testing on XR-DAVID, as well as several datasets from the literature, indicate a significant gain in prediction performance compared to existing metrics. ColorVideoVDP opens the doors to many novel applications that require the joint automated spatiotemporal assessment of luminance and color distortions, including video streaming, display specification, and design, visual comparison of results, and perceptually-guided quality optimization. The code for the metric can be found at https://github.com/gfxdisp/ColorVideoVDP.

Visual function and retinal thickness in children with type 1 diabetes mellitus

ABSTRACT Clinical Relevance The possibility that changes in blue–yellow visual thresholds and some retinal thickness measures in children with diabetes mellitus may be observed before any visible fundus changes points to the possibility of these measures being a useful predictor that the risks of diabetic retinopathy are higher in some children than in others. Introduction Previous studies showed mixed results on chromatic and achromatic contrast sensitivity early in the course of diabetes mellitus, and the findings of these studies may have been influenced by a lack of experimental sensitivity to visual deficits, a bias towards tritan-like errors or the cognitive demands of the tests and variations in sample composition. The purpose of this study was to evaluate colour and contrast thresholds and retinal thickness in children with type 1 diabetes mellitus compared with age-matched controls. Methods A prospective case–control study was carried out on 9–14-year-old children with type 1 diabetes mellitus (49 cases) and age matched controls (49) in which isoluminant red–green and blue–yellow and achromatic luminance contrast thresholds were measured. Fundus photography was used to grade diabetic retinopathy. Retinal thickness parameters were measured using optical coherence tomography. Data on the duration of diabetes mellitus, glycaemic control (HbA1c), blood glucose level, body mass index, blood pressure and blood oxygenation at the time of testing were obtained. Results The cases mostly had poorly controlled diabetes, HbA1c 8.6% (6.4–12.8%), for an average (range) duration of 5 (0.4–12) years. The cases had significantly higher blue–yellow thresholds (p = 0.02) and greater total retinal and inner retinal thickness (p < 0.05) than controls. No cases had diabetic retinopathy. Within the cases, poorer visual function and systemic health measures were associated with thinner retinal structures and greater global loss volume percentage in the ganglion cell complex. Conclusion Blue–yellow thresholds of cases were raised compared to normal. Within the cases, higher luminance contrast thresholds were also associated with, mostly, ganglion cell complex reductions.

Riemannian color difference metric for spatial sinusoidal color variations

Several studies report on the sensitivity of human vision to static spatial sinusoidal achromatic and chromatic contrast variations. However, a Riemannian color difference metric, which includes the spatial and colorimetric properties of sinusoidal gratings, is lacking. Such a metric is important for various applications. Here we report on the development of a new Riemannian metric, for the prediction of detection ellipsoids in color space, for spatial sinusoidal gratings as a function of the grating’s size, spatial frequency, luminance and chromaticity. The metric is based on measurements and models of achromatic and isoluminous chromatic contrast sensitivity functions available in literature, and the Riemannian metric for split fields which we reported earlier. We find adequate agreement with various data sets of experimental achromatic and isoluminous chromatic contrast sensitivity functions and with experimentally determined threshold ellipses of isoluminous chromatic Gabor gratings.

Applying Resampling and Visualization Methods in Factor Analysis to Model Human Spatial Vision.

Studies have reported different numbers of spatial frequency channels for chromatic and achromatic vision. To resolve the difference, we performed factor analysis, a multivariate modeling technique, on population data of achromatic and chromatic sensitivity. In addition, we included resampling and visualization methods to evaluate models from factor analysis. These routines are complex but widely useful. Therefore we have archived our analysis routines by building smCSF, an open-source software package in R (https://smin95.github.io/dataviz/). Data of 103 normally-sighted adults were analyzed. They included blue-yellow, red-green, and achromatic contrast sensitivity. To obtain the confidence interval of relevant statistical parameters, factor analysis was performed using a resampling method. Then exploratory models were developed. We then performed model selections by fitting them against the empirical data and quantifying the quality of the fits. During the exploratory factor analysis, different statistical tests supported different factor models. These could partially be reasons for why there have been conflicting reports. However, after the confirmatory analysis, we found that a model that included two spatial channels was adequate to approximate the chromatic sensitivity data, whereas that with three channels was so for the achromatic sensitivity data. Our findings provide novel insights about the spatial channels for chromatic and achromatic contrast sensitivity from population data. Also, the analysis and visualization routines have been archived in a computational package to boost the transparency and replicability of science.

Association between chromatic sensitivity and peripapillary retinal nerve fibre layer thickness in healthy eyes

Purpose: Knowledge of the retinal structure—chromatic sensitivity relationship of healthy eyes would be important for early detection of retinal neuropathies such as glaucoma. This study investigated the association between colour vision thresholds and the thicknesses of the retina in healthy young eyes.Methods: Measurements were made in 56 healthy eyes of 56 young subjects of mean age 25 ± 2.4 years (range 20–31 years), and distance visual acuity ≤0.1 logMAR. Chromatic sensitivity was measured using the Color Assessment and Diagnosis (CAD) test. The macular thicknesses of the central fovea, parafovea, and macular ganglion cell complex (mGCC), and the thickness of the peripapillary retinal nerve fibre layer (pRNFL) were measured by spectral‐domain optical coherence tomography (SD‐OCT). Relationships between red‐green (RG) and yellow‐blue (YB) thresholds and the retinal thicknesses were examined through Spearman correlation.Results: Significant correlations were detected between pRNFL thickness, in the inferior hemisphere, and the RG and YB‐thresholds (r = −0.30, p = 0.03 and r = −0.31, p = 0.02; respectively). Decreased pRNFL thickness correlated with worse colour thresholds. No significant correlations were found between macular thicknesses and colour thresholds.Conclusions: Greater inferior pRNFL thickness was related to better chromatic sensitivity in healthy eyes of young subjects.

Effect of pre‐receptor filters on chromatic sensitivity and other visual functions in the eye

Spatial and spectral information carried by light emitted from visual displays is filtered as it passes through spectacle lenses in front of the eye as well as by filters within the eye. The image forming and the spectral transmittance properties of the elements involved in image formation in the eye cause changes in both the luminance and colour contrast in retinal images. In addition, the amount of light that reaches the retina is always reduced by pre‐receptor filters and this reduction in retinal illuminance can cause diminished retinal sensitivity to both luminance and colour contrast. Of particular interest are the use of coloured lenses in front of the eye to reduce the short‐wavelength content of the light that reaches the retina and the use of ‘notch’ filters to absorb light selectively in certain wavebands, with inevitable consequences on both the luminance and chromatic contrast of retinal images. The presence of variant cone‐pigments in some subjects or the complete absence of one class of cone pigment in others, can also produce large deviations in both luminance and colour contrast signals that can either enhance or diminish the conspicuity of coloured objects (doi: 10.1364/josaa.22.000017).The purpose of this study was to develop and validate a model of red/green (RG) and yellow/blue (YB) colour discrimination that can also predict how chromatic sensitivity is affected by the use of spectrally‐selective, spectacle lenses or by filters internal to the eye, when viewing visual displays. We examined how RG and YB colour thresholds relate to the corresponding cone contrasts needed for detection of colour differences. The results reveal an invariance of cone contrasts at threshold for different states of chromatic adaptation. In other words, colour contrast signals at threshold follow Weber's law (doi: 10.1111/j.1475‐1313.2010.00773.x). A combination of RG and YB colour signals is needed to predict the colour threshold ellipse. The model relies on these findings and predicts how coloured filters external to the eye as well as pre‐receptor filters within the eye alter the measured RG and YB colour thresholds. Model predictions have been compared against measured colour thresholds using the CAD test (doi: 10.1093/bmb/ldx007). A number of experiments were carried out when the visual display was viewed with no lens in front of the eye or through a number of tinted lenses designed to absorb progressively more blue light.The effects of pre‐receptor filters within the eye such as the lens and the macular pigment have also been investigated. The results show that ‘blue‐blocking’ filters employed in typical spectacle lenses used with daylight illumination cause only small shifts, approximately along the daylight locus, without any significant loss of either RG or YB chromatic sensitivity. ‘Orange’ lenses that absorb greater amounts of short‐wavelength light cause a significant increase in YB thresholds, but have little or no effect on RG thresholds. More surprisingly, the model also predicts that the optical density of the macular pigment has little or no effect on the size of the colour threshold ellipse.Keywords: colour, cone contrasts, blue light, CAD test.

The value of assessing progressive loss of red/green and yellow/blue chromatic sensitivity in glaucoma

*Correspondence: franziska.rauscher@medizin.uni-leipzig.deThe value of assessing progressive loss of red/green and yellow/blue chromatic sensitivity in glaucoma.The study employed visual stimuli designed to isolate and reveal how spatial, temporal and chromatic aspects of visual processing change from normal sensitivity to extreme loss in glaucoma. The experiments carried out examined the fovea and each of four paracentral retinal regions, arranged diagonally away from fixation at an eccentricity of 6°. Spatial vision was investigated by measuring both absolute detection thresholds and functional contrast sensitivity using Landolt ring stimuli (Ophthalmic Physiol Opt 2022;42:1363–1378). These psychophysical tests based on Landolt C optotypes measure both detection thresholds and gap orientation discrimination thresholds in the same experiments using randomly interleaved test locations. First‐order motion perception was examined by using moving stimuli embedded in static luminance contrast noise. Red/green (RG) and yellow/blue (YB) colour thresholds were measured with the Colour Assessment and Diagnosis (CAD) test, which makes use of random, dynamic luminance contrast noise (±45%) to isolate the use of RG and YB colour signals. Subjects were normal controls (n = 65) and glaucoma patients with binocular visual field defects (n = 15) classified based on their Humphrey Field Analyser mean deviation (MD) scores. The impairment of visual function varied depending on the stimulus attribute and location tested. The severity of loss increased monotonically with disease progression. For subjects with mild glaucoma (MD −0.01 to −6.00 dB), significantly more data points exceeded the upper normal threshold limits for the corresponding age for RG colour thresholds than for any other visual test (Br Med Bull 2017;122:51–77), followed by motion thresholds. This was particularly the case for the parafoveal locations when compared with the equivalent thresholds measured at the fovea. The results suggest that a multifaceted measure of binocular visual performance based on foveal and parafoveal tests of RG colour vision and first‐order motion, can best inform on the progression of glaucoma and the overall loss of visual performance for comparison with other measures of changes in the quality of life in patients with glaucoma.Keywords: Glaucoma, Visual function, Colour vision, Motion detection, Contrast, Acuity, Colour Assessment and Diagnosis, CAD.

New methods for detection of early loss of rapid flicker, spatial vision, and chromatic sensitivity in glaucoma

Purpose: To examine whether ocular hypertension and early glaucoma cause measurable changes in spatial vision, rapid flicker sensitivity and red/green and yellow/blue colour vision.Methods: We examined subjects aged 40 to 78 years with ocular hypertension (OHT), primary open angle glaucoma (POAG), normal tension glaucoma (NTG), and age matched controls. Monocular best corrected visual acuity (BCVA) was tested using EDTRS charts.VA and Functional Contrast Sensitivity (FCS) with Landolt ring stimuli of both positive and negative contrast were also measured monocularly with the Acuity‐Plus test. Red/green (RG) and yellow/blue (YB) colour thresholds were measured with the Colour Assessment and Diagnostics (CAD) test. Photopic (cone‐enhanced) and mesopic (rod‐enhanced) rapid flicker thresholds were measured at the fovea (0°) and at an eccentricity of 5° in each of the four quadrants using the Flicker‐Plus test. All subjects had their visual fields measured using the Humphrey visual field analyser (24–2 SITA standard protocol). Peripapillary retinal nerve fibre layer thickness (pRNFLT) and macular retinal layer thicknesses were measured using the Heidelberg Spectralis OCT.Results: The mean, logMAR BCVA for OHT patients was 0.00 and for POAG and NTG patients was −0.10 and 0.10, respectively. For control participants, mean logMAR BCVA was 0.00. Mean pRNFL thickness was significantly reduced for glaucoma patients (59.5 ± 7.7 μm) as compared to control subjects (94.6 ± 5.13 μm) and OHT patients (115.5 ± 3.5 μm). POAG and NTG patients showed significantly higher colour thresholds (mean red/green threshold 4.97, blue/yellow 5.28 CAD units) compared to control subjects (mean red/green threshold 2.07, blue/yellow 1.62 CAD units). One OHT patient showed acquired loss of blue/yellow colour vision (3.23 CAD units), with normal VA. The Acuity‐Plus test which employs briefly presented stimuli, also revealed significant loss of VA (3.78 and 3.64 min of arc) and FCS (141.32% and 100%) in an NTG patient, with results outside normal limits for both positive and negative contrast, respectively. Central FCS was higher and outside the normal age limits for POAG patients (73.41% and 89.27%) as compared to age matched controls (61.27% and 33.63%). OHT patients, on the other hand, produced FCS thresholds within normal limits. Flicker modulation thresholds were higher for both rod and cone enhanced stimulus conditions in glaucoma and OHT patients, when compared to control subjects.Conclusion: The results reveal non‐uniform, losses that can affect selectively each of the visual attributes examined in this study. These preliminary findings suggest that in addition to VA and FCS tests, colour vision and rapid flicker sensitivity should also be examined in patients with optic nerve pathologies like glaucoma.

The use of colour and rapid flicker sensitivity tests for early detection and monitoring of vision changes in diabetes

Aims/Purpose: There is increasing evidence of impaired visual function prior to the presence of diabetic retinopathy (DR), yet current diagnostic criteria and treatment onset rely mostly on retinal vascular changes. In this study we measured changes in chromatic and rapid flicker sensitivity in subjects with prediabetes and diabetes to establish the value of such tests as a potential biomarker in the detecting early diabetes.Methods: Three groups of subjects were recruited: 1) normal controls, 2) subjects with prediabetes (HbA1c between 42‐47 mmol/mol), and 3) diabetics (with or without DR). Monocular cone‐ and rod‐enhanced flicker modulation thresholds (FMTs) were measured at the point of regard and in four parafoveal (5°) locations using the Flicker‐Plus test. Monocular red/green (RG) and yellow/blue (YB) colour thresholds were also measured at the fovea using the Colour Assessment and Diagnosis (CAD) test.Results: Both the RG and YB thresholds, as well as rod‐ and cone‐mediated flicker thresholds were significantly higher in both diabetic and prediabetic groups (when compared with healthy controls). When examined individually, ~50% and ~ 63% of the prediabetics and ~ 58% and ~ 70% of the diabetics revealed abnormal RG and YB thresholds, respectively (when compared with the upper normal limits for the corresponding age). HBA1c levels also revealed a positive trend with increasing RG and YB thresholds in the prediabetic group.Conclusions: In agreement with previous findings, both colour and FMTs are affected in patients diagnosed with diabetes. Unexpectedly, those deemed prediabetic that do not meet the clinical criteria for diabetes exhibit considerable loss of both colour and flicker sensitivity. The results suggest that colour and rapid flicker tests can provide useful information when screening for diabetes.

Effect of macular pigment optical density on Yellow‐Blue and Red‐Green colour discrimination

The macular pigment (MP) absorbs preferentially short‐wavelength light and its narrow spectral absorption curve overlaps mostly the spectral responsivity function of S‐cones, although the M‐ and L‐cones are also affected. The MP is restricted to the centre ~14° disc region of the retina with a peak optical density (OD) at the centre in the range 0 to 1 log units. Although the macular pigment optical density (MPOD) decreases rapidly with increasing eccentricity, carotenoid supplementation studies reveal a large uptake of lutein and zeaxanthin, particularly in the parafoveal region and covering the centre ~6° to 8° of the fovea (doi: 10.1111/j.1475‐1313.2006.00386.x).Since the MP contributes to the reduced excitation of S‐cones, it is of interest to examine the effects MP may have on colour vision, particularly when ‘blue‐blocking’ lenses are also involved. We studied the relationship between MPOD and red/green (RG) and yellow/blue (YB) colour thresholds by measuring the spatial distribution of the MP in normal trichromats using the Macular Assessment Profile (MAP) test (doi: 10.1111/j.1475‐1313.2010.00748.x) and the corresponding RG and YB colour thresholds using the CAD test (doi:10.1093/bmb/ldx007).The results show negligible correlation between peak MPOD and YB thresholds and a small, but significant negative correlation with RG thresholds. This observation is of interest since increased MPOD values result in lower RG thresholds. The same experiment was also carried out in subjects with significantly larger amounts of MP following 3 months of supplementation with carotenoids. When averaged over the centre disc region of 2.5° and then over a parafoveal annulus between 2.5° and 4°, the corresponding, mean MP densities show only negligible correlations with RG and YB colour thresholds. To explain these findings, we calculated the expected cone‐contrasts for S‐, M‐ and L‐cones for chromatic displacements defined by the threshold ellipse. The results show that MP absorption of short‐wavelength light has a negligible effect on S‐cone contrast over the whole range of displacement directions associated with the colour threshold ellipse. Repeated calculations with simulated MPOD values up to 1 log unit, produced the same result. More recently, we modelled the effects ‘blue‐blocking’ lenses and pre‐receptor filters in the eye to explore changes to the orientation and the size of the colour threshold ellipse. The model predicts well the measured RG and YB thresholds and confirms the absence of significant threshold changes for MPOD values in the range 0 to 1 log unit. The model does, however, predict systematic changes in the orientation of the major axis (i.e., the tritan axis) and MPOD. These observations are of interest since the orientation of the major axis of the best‐fit ellipse appears to decrease monotonically from ~75° to 55°, as MPOD increases from very small values up to a peak OD of 1 log unit. More work is needed to investigate how YB and RG chromatic sensitivity is affected by combined absorption of short‐wavelength light by MPOD, the lens of the eye and ‘blue‐blocking’ spectacle lenses.

Poster Session I: Diurnal variations in luminance and chromatic contrast sensitivity.

Sensory motor performance typically peaks in the evening, influenced by observer's diurnal arousal level. The arousal level is regulated by neurons in the brainstem, which form connections extending throughout the brain but the connection is highly heterogeneous. The dorsal visual pathway and superior colliculus receive a dense projections, while the ventral visual pathway and lateral geniculate nucleus receive fewer. The former primarily deal with stimuli determined by luminance contrast, while the latter handle chromatic information. How such heterogeneous projection in the brain impacts on human visual detection performance has yet to be understood. In this study, we measured the diurnal variation in human contrast sensitivity and investigated whether changes differed between luminance and chromatic stimuli. Result revealed that as the time of day progressed, sensitivity to luminance contrast improved, while sensitivity to color contrast deteriorated.

Diurnal Modulation of Pupillary Light Reflex

Pupillary light reflex is mediated by intrinsic photosensitive retinal ganglion cells (ipRGCs) and the midbrain in human brain. The cells ipRGCs are highly sensitive to blue light (~ 480 nm). In this study, we evaluated diurnal changes in chromatic sensitivity of ipRGCs by measuring pupillary light reflex. The data showed that sensitivity to red and green light increased from morning to evening, but that to blue decreased from noon to evening. These results indicate that sensitivity of ipRGCs may decreases in evening.

Melanopsin enhances image persistence

Contributions of the inner retinal photopigment melanopsin to human visual perception are incompletely understood. Here, we use a four-primary display to produce stimuli differing in melanopsin versus cone contrast in psychophysical paradigms in eight subjects with normal color vision. We address two predictions from electrophysiological recordings of the melanopsin system in non-human mammals: melanopsin influences color and/or supports image persistence under visual fixation. We first construct chromatic contrast sensitivity contours for stimuli differing in melanopsin excitation presented as a central annulus (10°) or peripheral (22.5°) spot. We find that although including melanopsin contrast produces modest changes in the average chromatic coordinates in both eccentricities, this occurs equally at low (0.5Hz) and higher (3.75Hz) temporal frequencies, arguing that it reflects divergence in cone spectral sensitivity in our participants from that captured in standardized cone fundamentals rather than a melanopsin contribution to color. We continue to ask whether the established ability of melanopsin to sustain firing of visual neurons under extended light exposure has a visual correlate, using the optical illusion of Troxler fading in which blurred spots in periphery disappear during visual fixation. We find that introducing additional melanopsin contrast (+28% Michelson contrast) to either bright or dark spots increases fading latency by 35%± 8.8% and 41%± 13.6%, respectively. Our data argue that the primary contribution of melanopsin to perception under these conditions isnot to provide a color percept but rather to enhance persistence of low spatial frequency patterns during visual fixation.

A Study of Spatial Chromatic Contrast Sensitivity based on Different Colors, Luminance, and Stimulus Patterns

A Study of Spatial Chromatic Contrast Sensitivity based on Different Colors, Luminance, and Stimulus Patterns

Spatial and chromatic sensitivity of the primary visual cortex at the center-of-gaze

Spatial and chromatic sensitivity of the primary visual cortex at the center-of-gaze

Achromatic and chromatic contrast discrimination in patients with type 2 diabetes

Effects of type 2 diabetes on achromatic and chromatic contrast sensitivity (CS) are still controversial. In this study, we aimed to investigate CS in patients without diabetic retinopathy (no-DR) and in those with non-proliferative DR (NPDR) and proliferative DR (PDR) using psychophysical methods with transient and sustained achromatic stimuli and color patches. Achromatic CS was measured with the pulsed pedestal (PP) paradigm (7, 12, and 19 cd/m2) and pedestal-△-pedestal (P-△-P) paradigm (11.4, 18, and 28.5 cd/m2). A chromatic discrimination paradigm that assesses protan, deutan, and tritan color vision was adopted. Forty-two patients (no-DR n = 24, NPDR n = 12, PDR = 6; male n = 22, mean age = 58.1 y/o) and 38 controls (male n = 18, mean age = 53.4 y/o) participated. In patients, mean thresholds were higher than in controls and linear trends were significant in most conditions. For the PP paradigm, differences were significant in the PDR and NPDR groups in the 7 and 12 cd/m2 condition. For the P-△-P paradigm, differences were only significant in the PDR group in the 11 cd/m2 condition. Chromatic contrast loss was significant in the PDR group along the protan, deutan and tritan axes. The results suggest independent involvements of achromatic and chromatic CS in diabetic patients.

Increasing spatial frequency of S-cone defined gratings reduces their visibility and brain response more than for gratings defined by L-M cone contrast

Chromatic sensitivity reduces as spatial frequency increases. Here, we explore the behavioural and neuronal responses to chromatic stimuli at two spatial frequencies for which the difference in sensitivity will be greater for S-cone than L-M stimuli. Luminance artefacts were removed using the Random Luminance Modulation (RLM) technique. As expected, doubling the spatial frequency increased the detection threshold more for S-cone than for isoluminant L-M gratings. We then used fMRI to measure the cortical BOLD responses to the same two chromatic stimuli (S and L-M) at the same two spatial frequencies. Responses were measured in six visual areas (V1, V2, V3, V3a, hV4, TO1/2). We found a significant interaction between spatial frequency in V1, V2 and V4 suggesting that the behaviourally observed increase in contrast threshold for high spatial frequency S-cone stimuli is reflected in these retinotopic areas. Our measurements show that neural responses consistent with psychophysical behaviour in a colour detection task can be observed as early as primary visual cortex.

Continuous motion tracking to rapidly estimate chromatic contrast sensitivity and identify color deficiency

Continuous motion tracking to rapidly estimate chromatic contrast sensitivity and identify color deficiency