Objective: To compare the biofilm of Candida albicans found in bases of total dentures. Study Design: Four groups of users of total denture were grouped according to time of use: 1 to 2 months (n = 13), 3 to 9 months (n = 13), 30 to 39 months (n = 13), and more than 39 months (n = 13). After clinical examination and physical evaluation of the prosthesis, samples of the oral mucosa, hard palate as well as the surface total denture were collected with sterilized swabs, and sown in CHROMagar Candida medium to identify species at 37°C for 48 hours. The identification of the species was using the API 20 method. Results: The results revealed the presence of Candida albicans and other types of strains in the biofilm of the total denture with more than 3 months of use, but without a significant difference between the presence of xerostomia and the type of hygiene, with the presence of strains. Conclusion: There is a significant difference between the time of total denture use and the amount of colony-forming units. Objective: To compare the biofilm of Candida albicans found in bases of total dentures. Study Design: Four groups of users of total denture were grouped according to time of use: 1 to 2 months (n = 13), 3 to 9 months (n = 13), 30 to 39 months (n = 13), and more than 39 months (n = 13). After clinical examination and physical evaluation of the prosthesis, samples of the oral mucosa, hard palate as well as the surface total denture were collected with sterilized swabs, and sown in CHROMagar Candida medium to identify species at 37°C for 48 hours. The identification of the species was using the API 20 method. Results: The results revealed the presence of Candida albicans and other types of strains in the biofilm of the total denture with more than 3 months of use, but without a significant difference between the presence of xerostomia and the type of hygiene, with the presence of strains. Conclusion: There is a significant difference between the time of total denture use and the amount of colony-forming units.